#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P29469	uniprotkb:P24279	intact:EBI-10533|ensemblfungi:YBL023C|uniprotkb:D6VPX7	intact:EBI-10541|ensemblfungi:YEL032W|uniprotkb:D3DLL7	psi-mi:mcm2_yeast(display_long)|uniprotkb:MCM2(gene name)|psi-mi:MCM2(display_short)|uniprotkb:YBL023C(locus name)|uniprotkb:YBL0438(orf name)|uniprotkb:Minichromosome maintenance protein 2(gene name synonym)	psi-mi:mcm3_yeast(display_long)|uniprotkb:MCM3(gene name)|psi-mi:MCM3(display_short)|uniprotkb:YEL032W(locus name)|uniprotkb:SYGP-ORF23(orf name)|uniprotkb:Minichromosome maintenance protein 3(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	On et al. (2014)	pubmed:24566989|imex:IM-22944	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551191|imex:IM-22944-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009530.1|sgd:S000000119|ensemblfungi:YBL023C(gene)|ensemblfungi:YBL023C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1905775"(negative regulation of DNA helicase activity)|interpro:IPR001208(MCM)|interpro:IPR008045(MCM protein 2)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|go:"GO:1902975"(mitotic DNA replication initiation)|rcsb pdb:6EYC|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:6F0L|mint:P29469|dip:DIP-2291N	refseq:NP_010882.1|sgd:S000000758|go:"GO:0005524"(ATP binding)|go:"GO:0003697"(single-stranded DNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006348"|go:"GO:0030466"(silent mating-type cassette heterochromatin assembly)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1902975"(mitotic DNA replication initiation)|go:"GO:1904931"(MCM complex binding)|interpro:IPR001208(MCM)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR008046(MCM protein 3)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:6EYC|rcsb pdb:6F0L|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGC|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:5XF8|ensemblfungi:YEL032W(gene)|ensemblfungi:YEL032W_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0000781"(chromosome, telomeric region)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|mint:P24279|dip:DIP-2407N	-	crc64:F38FF682581B0EC0	crc64:43DD4DACAF4456DC	figure legend:f1d f1e|comment:"DNA-bound  Mcm2-7 complexes were released from the beads by EcoRI digestion  as previously described (Remus et al, 2009) and applied directly  onto carbon-coated copper grids for single particle EM analysis after  negative staining. Fig 1D shows that Mcm2-7 double hexamers were  readily observed in both the treated and untreated samples and,  more significantly, Mcm2-7 single hexamers were not found in  either sample. This indicates that DDK phosphorylation was not  sufficient to cause efficient dissociation of the Mcm2-7 double  hexamers despite the extensive phosphorylation of virtually all the  Mcm4 and 6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:+XEStaQQNnAcfUIf1BVvFLgKG5k559292	rogid:OsFbdaTeE1vPQObriGt5M6mTPzk559292	intact-crc:9C2926B8A1AFDA89|rigid:3fGBygN4HzZBermr8ECUtCxTOoM	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P29469	uniprotkb:P33991	intact:EBI-10533|ensemblfungi:YBL023C|uniprotkb:D6VPX7	intact:EBI-374938|uniprotkb:Q8NEH1|uniprotkb:Q99658|ensembl:ENSP00000262105|ensembl:ENSP00000496964	psi-mi:mcm2_yeast(display_long)|uniprotkb:MCM2(gene name)|psi-mi:MCM2(display_short)|uniprotkb:YBL023C(locus name)|uniprotkb:YBL0438(orf name)|uniprotkb:Minichromosome maintenance protein 2(gene name synonym)	psi-mi:mcm4_human(display_long)|uniprotkb:MCM4(gene name)|psi-mi:MCM4(display_short)|uniprotkb:CDC21(gene name synonym)|uniprotkb:CDC21 homolog(gene name synonym)|uniprotkb:P1-CDC21(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	On et al. (2014)	pubmed:24566989|imex:IM-22944	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551191|imex:IM-22944-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009530.1|sgd:S000000119|ensemblfungi:YBL023C(gene)|ensemblfungi:YBL023C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1905775"(negative regulation of DNA helicase activity)|interpro:IPR001208(MCM)|interpro:IPR008045(MCM protein 2)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|go:"GO:1902975"(mitotic DNA replication initiation)|rcsb pdb:6EYC|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:6F0L|mint:P29469|dip:DIP-2291N	refseq:NP_005905.2|refseq:NP_877423.1|ensembl:ENSG00000104738(gene)|ensembl:ENST00000262105(transcript)|ensembl:ENST00000649973(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0000781"(chromosome, telomeric region)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0003697"(single-stranded DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0006260"(DNA replication)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0016020"(membrane)|go:"GO:0042555"(MCM complex)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1902975"(mitotic DNA replication initiation)|interpro:IPR001208(MCM)|interpro:IPR008047(MCM protein 4)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|mint:P33991|rcsb pdb:6XTX|rcsb pdb:6XTY|reactome:R-HSA-176187|reactome:R-HSA-176974|reactome:R-HSA-68867|reactome:R-HSA-68949|reactome:R-HSA-68962|reactome:R-HSA-69052|dip:DIP-31729N	-	crc64:F38FF682581B0EC0	-	figure legend:f1d f1e|comment:"DNA-bound  Mcm2-7 complexes were released from the beads by EcoRI digestion  as previously described (Remus et al, 2009) and applied directly  onto carbon-coated copper grids for single particle EM analysis after  negative staining. Fig 1D shows that Mcm2-7 double hexamers were  readily observed in both the treated and untreated samples and,  more significantly, Mcm2-7 single hexamers were not found in  either sample. This indicates that DDK phosphorylation was not  sufficient to cause efficient dissociation of the Mcm2-7 double  hexamers despite the extensive phosphorylation of virtually all the  Mcm4 and 6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:+XEStaQQNnAcfUIf1BVvFLgKG5k559292	rogid:emFkamsHmwBPzoJnPcaL4SaqAlU9606	intact-crc:9C2926B8A1AFDA89|rigid:3fGBygN4HzZBermr8ECUtCxTOoM	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P29469	uniprotkb:P29496	intact:EBI-10533|ensemblfungi:YBL023C|uniprotkb:D6VPX7	intact:EBI-10549|ensemblfungi:YLR274W|uniprotkb:D6VYS1	psi-mi:mcm2_yeast(display_long)|uniprotkb:MCM2(gene name)|psi-mi:MCM2(display_short)|uniprotkb:YBL023C(locus name)|uniprotkb:YBL0438(orf name)|uniprotkb:Minichromosome maintenance protein 2(gene name synonym)	psi-mi:mcm5_yeast(display_long)|uniprotkb:MCM5(gene name)|psi-mi:MCM5(display_short)|uniprotkb:CDC46(gene name synonym)|uniprotkb:YLR274W(locus name)|uniprotkb:L9328.1(orf name)|uniprotkb:Cell division control protein 46(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	On et al. (2014)	pubmed:24566989|imex:IM-22944	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551191|imex:IM-22944-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009530.1|sgd:S000000119|ensemblfungi:YBL023C(gene)|ensemblfungi:YBL023C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1905775"(negative regulation of DNA helicase activity)|interpro:IPR001208(MCM)|interpro:IPR008045(MCM protein 2)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|go:"GO:1902975"(mitotic DNA replication initiation)|rcsb pdb:6EYC|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:6F0L|mint:P29469|dip:DIP-2291N	refseq:NP_013376.1|sgd:S000004264|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0031507"(heterochromatin assembly)|go:"GO:0031939"(obsolete negative regulation of chromatin silencing at telomere)|go:"GO:0033260"(nuclear DNA replication)|go:"GO:0042555"(MCM complex)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001208(MCM)|interpro:IPR008048(MCM protein 5)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|go:"GO:0030174"(regulation of DNA-dependent DNA replication initiation)|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|rcsb pdb:6EYC|rcsb pdb:6F0L|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:3JC5|rcsb pdb:6WGG|rcsb pdb:6WGI|ensemblfungi:YLR274W(gene)|ensemblfungi:YLR274W_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0000781"(chromosome, telomeric region)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006348"|reactome:R-SCE-68949|reactome:R-SCE-69052|reactome:R-SCE-68962|dip:DIP-2406N	-	crc64:F38FF682581B0EC0	crc64:01D9DE208A091CF2	figure legend:f1d f1e|comment:"DNA-bound  Mcm2-7 complexes were released from the beads by EcoRI digestion  as previously described (Remus et al, 2009) and applied directly  onto carbon-coated copper grids for single particle EM analysis after  negative staining. Fig 1D shows that Mcm2-7 double hexamers were  readily observed in both the treated and untreated samples and,  more significantly, Mcm2-7 single hexamers were not found in  either sample. This indicates that DDK phosphorylation was not  sufficient to cause efficient dissociation of the Mcm2-7 double  hexamers despite the extensive phosphorylation of virtually all the  Mcm4 and 6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:+XEStaQQNnAcfUIf1BVvFLgKG5k559292	rogid:a8UgYOklJ5vWqhA0hbQTCWNQg0Y559292	intact-crc:9C2926B8A1AFDA89|rigid:3fGBygN4HzZBermr8ECUtCxTOoM	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P29469	uniprotkb:P53091	intact:EBI-10533|ensemblfungi:YBL023C|uniprotkb:D6VPX7	intact:EBI-10556|uniprotkb:Q870M9|ensemblfungi:YGL201C|uniprotkb:D6VTV3	psi-mi:mcm2_yeast(display_long)|uniprotkb:MCM2(gene name)|psi-mi:MCM2(display_short)|uniprotkb:YBL023C(locus name)|uniprotkb:YBL0438(orf name)|uniprotkb:Minichromosome maintenance protein 2(gene name synonym)	psi-mi:mcm6_yeast(display_long)|uniprotkb:YGL201C(locus name)|uniprotkb:MCM6(gene name)|psi-mi:MCM6(display_short)|uniprotkb:Minichromosome maintenance protein 6(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	On et al. (2014)	pubmed:24566989|imex:IM-22944	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551191|imex:IM-22944-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009530.1|sgd:S000000119|ensemblfungi:YBL023C(gene)|ensemblfungi:YBL023C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1905775"(negative regulation of DNA helicase activity)|interpro:IPR001208(MCM)|interpro:IPR008045(MCM protein 2)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|go:"GO:1902975"(mitotic DNA replication initiation)|rcsb pdb:6EYC|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:6F0L|mint:P29469|dip:DIP-2291N	ensemblfungi:YGL201C(gene)|refseq:NP_011314.2|sgd:S000003169|ensemblfungi:YGL201C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0003697"(single-stranded DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0017116"(single-stranded DNA helicase activity)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0071162"(CMG complex)|go:"GO:0097373"(MCM core complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1902969"(mitotic DNA replication)|interpro:IPR001208(MCM)|interpro:IPR008049(MCM protein 6)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041024|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5XF8|rcsb pdb:6EYC|rcsb pdb:6F0L|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:5V8F|mint:P53091|dip:DIP-1294N	-	crc64:F38FF682581B0EC0	crc64:03AB793134E64A50	figure legend:f1d f1e|comment:"DNA-bound  Mcm2-7 complexes were released from the beads by EcoRI digestion  as previously described (Remus et al, 2009) and applied directly  onto carbon-coated copper grids for single particle EM analysis after  negative staining. Fig 1D shows that Mcm2-7 double hexamers were  readily observed in both the treated and untreated samples and,  more significantly, Mcm2-7 single hexamers were not found in  either sample. This indicates that DDK phosphorylation was not  sufficient to cause efficient dissociation of the Mcm2-7 double  hexamers despite the extensive phosphorylation of virtually all the  Mcm4 and 6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:+XEStaQQNnAcfUIf1BVvFLgKG5k559292	rogid:b2Pak0d6eENHIzSFaBge/kPQceU559292	intact-crc:9C2926B8A1AFDA89|rigid:3fGBygN4HzZBermr8ECUtCxTOoM	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P29469	uniprotkb:P38132	intact:EBI-10533|ensemblfungi:YBL023C|uniprotkb:D6VPX7	intact:EBI-4300|uniprotkb:D6VQK0|ensemblfungi:YBR202W	psi-mi:mcm2_yeast(display_long)|uniprotkb:MCM2(gene name)|psi-mi:MCM2(display_short)|uniprotkb:YBL023C(locus name)|uniprotkb:YBL0438(orf name)|uniprotkb:Minichromosome maintenance protein 2(gene name synonym)	psi-mi:mcm7_yeast(display_long)|uniprotkb:MCM7(gene name)|psi-mi:MCM7(display_short)|uniprotkb:YBR202W(locus name)|uniprotkb:YBR1441(orf name)|uniprotkb:CDC47(gene name synonym)|uniprotkb:Cell division control protein 47(gene name synonym)|uniprotkb:Minichromosome maintenance protein 7(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	On et al. (2014)	pubmed:24566989|imex:IM-22944	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9551191|imex:IM-22944-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009530.1|sgd:S000000119|ensemblfungi:YBL023C(gene)|ensemblfungi:YBL023C_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0042555"(MCM complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0071162"(CMG complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1905775"(negative regulation of DNA helicase activity)|interpro:IPR001208(MCM)|interpro:IPR008045(MCM protein 2)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|go:"GO:1902975"(mitotic DNA replication initiation)|rcsb pdb:6EYC|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052|rcsb pdb:6F0L|mint:P29469|dip:DIP-2291N	dip:DIP-2408N|sgd:S000000406|refseq:NP_009761.4|mint:P38132|ensemblfungi:YBR202W(gene)|ensemblfungi:YBR202W_mRNA(transcript)|go:"GO:0000727"(double-strand break repair via break-induced replication)|go:"GO:0000781"(chromosome, telomeric region)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005656"(nuclear pre-replicative complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006267"(pre-replicative complex assembly involved in nuclear cell cycle DNA replication)|go:"GO:0006268"(DNA unwinding involved in DNA replication)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0006271"(DNA strand elongation involved in DNA replication)|go:"GO:0006348"|go:"GO:0017116"(single-stranded DNA helicase activity)|go:"GO:0030466"(silent mating-type cassette heterochromatin assembly)|go:"GO:0031261"(DNA replication preinitiation complex)|go:"GO:0031298"(replication fork protection complex)|go:"GO:0033260"(nuclear DNA replication)|go:"GO:0042555"(MCM complex)|go:"GO:0071162"(CMG complex)|go:"GO:0097373"(MCM core complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1904931"(MCM complex binding)|interpro:IPR001208(MCM)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR008050(MCM protein 7)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR018525|interpro:IPR027417|interpro:IPR027925|interpro:IPR031327|interpro:IPR033762|interpro:IPR041562|rcsb pdb:3JA8|rcsb pdb:3JC5|rcsb pdb:3JC6|rcsb pdb:3JC7|rcsb pdb:5BK4|rcsb pdb:5U8S|rcsb pdb:5U8T|rcsb pdb:5V8F|rcsb pdb:5XF8|rcsb pdb:6EYC|rcsb pdb:6F0L|rcsb pdb:6HV9|rcsb pdb:6PTJ|rcsb pdb:6PTN|rcsb pdb:6PTO|rcsb pdb:6RQC|rcsb pdb:6SKL|rcsb pdb:6SKO|rcsb pdb:6U0M|rcsb pdb:6WGC|rcsb pdb:6WGF|rcsb pdb:6WGG|rcsb pdb:6WGI|reactome:R-SCE-68949|reactome:R-SCE-68962|reactome:R-SCE-69052	-	crc64:F38FF682581B0EC0	crc64:ADA66C719D96DB4A	figure legend:f1d f1e|comment:"DNA-bound  Mcm2-7 complexes were released from the beads by EcoRI digestion  as previously described (Remus et al, 2009) and applied directly  onto carbon-coated copper grids for single particle EM analysis after  negative staining. Fig 1D shows that Mcm2-7 double hexamers were  readily observed in both the treated and untreated samples and,  more significantly, Mcm2-7 single hexamers were not found in  either sample. This indicates that DDK phosphorylation was not  sufficient to cause efficient dissociation of the Mcm2-7 double  hexamers despite the extensive phosphorylation of virtually all the  Mcm4 and 6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/17	2014/10/16	rogid:+XEStaQQNnAcfUIf1BVvFLgKG5k559292	rogid:OJMt3vgMFg5we7rPkQSmkxbL1dA559292	intact-crc:9C2926B8A1AFDA89|rigid:3fGBygN4HzZBermr8ECUtCxTOoM	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)