#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P00750	uniprotkb:P00750	intact:EBI-5605520|uniprotkb:A8K022|uniprotkb:B2R8E8|uniprotkb:Q15103|uniprotkb:Q503B0|uniprotkb:Q6PJA5|uniprotkb:Q7Z7N2|uniprotkb:Q86YK8|uniprotkb:Q9BU99|uniprotkb:Q9BZW1|ensembl:ENSP00000220809|ensembl:ENSP00000392045|ensembl:ENSP00000504311	intact:EBI-5605520|uniprotkb:A8K022|uniprotkb:B2R8E8|uniprotkb:Q15103|uniprotkb:Q503B0|uniprotkb:Q6PJA5|uniprotkb:Q7Z7N2|uniprotkb:Q86YK8|uniprotkb:Q9BU99|uniprotkb:Q9BZW1|ensembl:ENSP00000220809|ensembl:ENSP00000392045|ensembl:ENSP00000504311	psi-mi:tpa_human(display_long)|uniprotkb:PLAT(gene name)|psi-mi:PLAT(display_short)	psi-mi:tpa_human(display_long)|uniprotkb:PLAT(gene name)|psi-mi:PLAT(display_short)	psi-mi:"MI:0038"(dynamic light scattering)	Tischer et al. (2014)	imex:IM-22212|pubmed:24506586	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9107376|imex:IM-22212-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001306118.1|refseq:NP_000921.1|refseq:NP_127509.1|ensembl:ENSG00000104368(gene)|ensembl:ENST00000220809(transcript)|ensembl:ENST00000429089(transcript)|ensembl:ENST00000679151(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006508"(proteolysis)|go:"GO:0007596"(blood coagulation)|go:"GO:0009986"(cell surface)|go:"GO:0014909"(smooth muscle cell migration)|go:"GO:0030141"(secretory granule)|go:"GO:0031639"(plasminogen activation)|go:"GO:0042730"(fibrinolysis)|go:"GO:0045177"(apical part of cell)|go:"GO:0045861"(negative regulation of proteolysis)|go:"GO:0048008"(platelet-derived growth factor receptor signaling pathway)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0060468"(prevention of polyspermy)|go:"GO:0070062"(extracellular exosome)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099183"(trans-synaptic signaling by BDNF, modulating synaptic transmission)|interpro:IPR000001(Kringle)|interpro:IPR000083(Fibronectin, type I)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR013806(Kringle-like fold)|interpro:IPR018056|interpro:IPR018114|interpro:IPR026280|interpro:IPR033116|interpro:IPR034811|interpro:IPR038178|interpro:IPR043504|rcsb pdb:1A5H|rcsb pdb:1BDA|rcsb pdb:1PK2|rcsb pdb:1PML|rcsb pdb:1RTF|rcsb pdb:1TPG|rcsb pdb:1TPK|rcsb pdb:1TPM|rcsb pdb:1TPN|rcsb pdb:5BRR|rcsb pdb:5ZLZ|reactome:R-HSA-186797|reactome:R-HSA-75205	refseq:NP_001306118.1|refseq:NP_000921.1|refseq:NP_127509.1|ensembl:ENSG00000104368(gene)|ensembl:ENST00000220809(transcript)|ensembl:ENST00000429089(transcript)|ensembl:ENST00000679151(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005737"(cytoplasm)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006508"(proteolysis)|go:"GO:0007596"(blood coagulation)|go:"GO:0009986"(cell surface)|go:"GO:0014909"(smooth muscle cell migration)|go:"GO:0030141"(secretory granule)|go:"GO:0031639"(plasminogen activation)|go:"GO:0042730"(fibrinolysis)|go:"GO:0045177"(apical part of cell)|go:"GO:0045861"(negative regulation of proteolysis)|go:"GO:0048008"(platelet-derived growth factor receptor signaling pathway)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0060468"(prevention of polyspermy)|go:"GO:0070062"(extracellular exosome)|go:"GO:0098685"(Schaffer collateral - CA1 synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099183"(trans-synaptic signaling by BDNF, modulating synaptic transmission)|interpro:IPR000001(Kringle)|interpro:IPR000083(Fibronectin, type I)|interpro:IPR000742(EGF-like, type 3)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR013806(Kringle-like fold)|interpro:IPR018056|interpro:IPR018114|interpro:IPR026280|interpro:IPR033116|interpro:IPR034811|interpro:IPR038178|interpro:IPR043504|rcsb pdb:1A5H|rcsb pdb:1BDA|rcsb pdb:1PK2|rcsb pdb:1PML|rcsb pdb:1RTF|rcsb pdb:1TPG|rcsb pdb:1TPK|rcsb pdb:1TPM|rcsb pdb:1TPN|rcsb pdb:5BRR|rcsb pdb:5ZLZ|reactome:R-HSA-186797|reactome:R-HSA-75205	-	-	-	figure legend:f5 f2b|comment:"The solubility maximum of CM-rPA in 2 M EMIMCl was also measured as a function  of incubation time (Fig. 2A; inset). The solubility of CM-rPA decreased over several  days and after 67 days of incubation, 1.3 ± 0.1 mg mL-1 CM-rPA was detected in the  soluble fraction. In addition to the solubility measurements, the state of CM-rPA in  solution was analyzed by dynamic light scattering measurements (Fig. 2B). From these  measurements a z-average value of 16.9 ± 0.5 nm was calculated for a sample which  was incubated 19 h in 2 M EMIMCl at 4 °C. As the determined z-average values are a  measure for the intensity weighed hydrodynamic diameter of particles, they indicate that  CM-rPA exists as an ensemble of small aggregated protein species in solution. After an  incubation of 67 days, an increase of the z-average value to 18.3 ± 0.1 was observed,  indicating a shift of the aggregates to larger sizes."|comment:"To test this hypothesis, we used dynamic light scattering to determine the  particle size distributions (PSDs) for 54 mg mL-1 tc-rPA in 5.4 M EMIMCl as a  function of time (Fig. 5). Since it was not possible to determine the exact viscosity of  the EMIMCl-solution containing a certain amount of tc-rPA, the first z-average was  adjusted to a diameter of 5.7 nm, which corresponded to globular, monomeric tc-rPA  with a size 39.6 kDa.  The measurement of the intensity weighed PSD showed a shift towards higher sizes  over time. This was also reflected by the z-average diameters which increased from 5.7  nm to approximately 75 nm within 214 h. Under the assumption of a globular shape for  the 75 nm particles such a z-average correlates to a hydrodynamic diameter of  approximately 12 MDa indicating that tc-rPA formed soluble aggregates after unfolding  in 5.4 M EMIMCl."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:YG0q3fS8FWhr/g9xuRyMM+oYcXo9606	rogid:YG0q3fS8FWhr/g9xuRyMM+oYcXo9606	intact-crc:EF539D1B428311ED|rigid:dUXis5hTlzjvpOU8RT9ZdHPK1aw	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)