#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P22888	uniprotkb:P22888	intact:EBI-9084084|uniprotkb:Q14751|uniprotkb:Q15996|uniprotkb:Q9UEW9|ensembl:ENSP00000294954	intact:EBI-9084084|uniprotkb:Q14751|uniprotkb:Q15996|uniprotkb:Q9UEW9|ensembl:ENSP00000294954	psi-mi:lshr_human(display_long)|uniprotkb:LHCGR(gene name)|psi-mi:LHCGR(display_short)|uniprotkb:LCGR(gene name synonym)|uniprotkb:LGR2(gene name synonym)|uniprotkb:LHRHR(gene name synonym)|uniprotkb:Luteinizing hormone receptor(gene name synonym)	psi-mi:lshr_human(display_long)|uniprotkb:LHCGR(gene name)|psi-mi:LHCGR(display_short)|uniprotkb:LCGR(gene name synonym)|uniprotkb:LGR2(gene name synonym)|uniprotkb:LHRHR(gene name synonym)|uniprotkb:Luteinizing hormone receptor(gene name synonym)	psi-mi:"MI:0052"(fluorescence correlation spectroscopy)	Grzesik et al. (2014)	pubmed:24438591|imex:IM-22077	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9084082|imex:IM-22077-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000224.2|ensembl:ENSG00000138039(gene)|ensembl:ENST00000294954(transcript)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0004964"(luteinizing hormone receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007200"(phospholipase C-activating G protein-coupled receptor signaling pathway)|go:"GO:0008528"(G protein-coupled peptide receptor activity)|go:"GO:0008584"(male gonad development)|go:"GO:0009755"(hormone-mediated signaling pathway)|go:"GO:0022602"(ovulation cycle process)|go:"GO:0030539"(male genitalia development)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0035472"(choriogonadotropin hormone receptor activity)|go:"GO:0038106"(choriogonadotropin hormone binding)|go:"GO:0042700"(luteinizing hormone signaling pathway)|go:"GO:0050890"(cognition)|go:"GO:0071371"(cellular response to gonadotropin stimulus)|go:"GO:0071373"(cellular response to luteinizing hormone stimulus)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR002131(Glycoprotein hormone receptor)|interpro:IPR002273(Lutropin-choriogonadotropic hormone receptor)|interpro:IPR026906|interpro:IPR032675|rcsb pdb:1LUT|rcsb pdb:1XUL|reactome:R-HSA-375281|reactome:R-HSA-418555|reactome:R-HSA-9660821|interpro:IPR017452(GPCR, rhodopsin-like superfamily)	refseq:NP_000224.2|ensembl:ENSG00000138039(gene)|ensembl:ENST00000294954(transcript)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0004964"(luteinizing hormone receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007200"(phospholipase C-activating G protein-coupled receptor signaling pathway)|go:"GO:0008528"(G protein-coupled peptide receptor activity)|go:"GO:0008584"(male gonad development)|go:"GO:0009755"(hormone-mediated signaling pathway)|go:"GO:0022602"(ovulation cycle process)|go:"GO:0030539"(male genitalia development)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0035472"(choriogonadotropin hormone receptor activity)|go:"GO:0038106"(choriogonadotropin hormone binding)|go:"GO:0042700"(luteinizing hormone signaling pathway)|go:"GO:0050890"(cognition)|go:"GO:0071371"(cellular response to gonadotropin stimulus)|go:"GO:0071373"(cellular response to luteinizing hormone stimulus)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR002131(Glycoprotein hormone receptor)|interpro:IPR002273(Lutropin-choriogonadotropic hormone receptor)|interpro:IPR026906|interpro:IPR032675|rcsb pdb:1LUT|rcsb pdb:1XUL|reactome:R-HSA-375281|reactome:R-HSA-418555|reactome:R-HSA-9660821|interpro:IPR017452(GPCR, rhodopsin-like superfamily)	go:"GO:0005886"(plasma membrane)	-	-	figure legend:f3|comment:"Using fluorescent cross-correlation spectroscopy (FCCS), we tested each co-expressed pair of  signaling- and binding-deficient mutants for significant cross-correlation. As a positive  control, we used wild type hLHR, and LHR-homodimerization was indeed confirmed in our  FCCS experiments. As a negative control, we used a membrane-attached, A kinase anchor  protein 18 alpha (AKAP18α-mCherry) construct, co-transfected with hLHR-delExon10. A  cross-correlation of 7% was determined, which is consistent with the cross-talk of the systemand indicates that the molecules do not interact. For each pair of deficient mutants, including  the hLHR-delExon10, there was significant cross-correlation at the basal cell membrane.  Figures 3B and 3C show representative, normalized auto- and cross-correlation curves of  GFP, mCherry-tagged receptor constructs hLHR-delExon10, mutant hLHR-C131R, and the  negative control with the AKAP18α construct.  It was observed that neither the introduced point mutations, such as Cys131Arg and  Lys605Glu, nor the complete lack of Exon10 has a significant influence on receptor  oligomerization (figure 3A). These results confirm that oligomerization of the hLHRdelExon10  is not disturbed by the lack of the exon10."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/01/14	2014/10/16	rogid:YoSJGoLeUq4Oj+xCVrb9XhHUcBg9606	rogid:YoSJGoLeUq4Oj+xCVrb9XhHUcBg9606	intact-crc:7F50AAF7E4196A5F|rigid:/FjUdxUC5hJYQ/EWWZCh9xIVWs0	false	mcherry fluorescent protein tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:P22888	uniprotkb:P22888	intact:EBI-9084084|uniprotkb:Q14751|uniprotkb:Q15996|uniprotkb:Q9UEW9|ensembl:ENSP00000294954	intact:EBI-9084084|uniprotkb:Q14751|uniprotkb:Q15996|uniprotkb:Q9UEW9|ensembl:ENSP00000294954	psi-mi:lshr_human(display_long)|uniprotkb:LHCGR(gene name)|psi-mi:LHCGR(display_short)|uniprotkb:LCGR(gene name synonym)|uniprotkb:LGR2(gene name synonym)|uniprotkb:LHRHR(gene name synonym)|uniprotkb:Luteinizing hormone receptor(gene name synonym)	psi-mi:lshr_human(display_long)|uniprotkb:LHCGR(gene name)|psi-mi:LHCGR(display_short)|uniprotkb:LCGR(gene name synonym)|uniprotkb:LGR2(gene name synonym)|uniprotkb:LHRHR(gene name synonym)|uniprotkb:Luteinizing hormone receptor(gene name synonym)	psi-mi:"MI:0663"(confocal microscopy)	Grzesik et al. (2014)	pubmed:24438591|imex:IM-22077	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9084157|imex:IM-22077-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000224.2|ensembl:ENSG00000138039(gene)|ensembl:ENST00000294954(transcript)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0004964"(luteinizing hormone receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007200"(phospholipase C-activating G protein-coupled receptor signaling pathway)|go:"GO:0008528"(G protein-coupled peptide receptor activity)|go:"GO:0008584"(male gonad development)|go:"GO:0009755"(hormone-mediated signaling pathway)|go:"GO:0022602"(ovulation cycle process)|go:"GO:0030539"(male genitalia development)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0035472"(choriogonadotropin hormone receptor activity)|go:"GO:0038106"(choriogonadotropin hormone binding)|go:"GO:0042700"(luteinizing hormone signaling pathway)|go:"GO:0050890"(cognition)|go:"GO:0071371"(cellular response to gonadotropin stimulus)|go:"GO:0071373"(cellular response to luteinizing hormone stimulus)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR002131(Glycoprotein hormone receptor)|interpro:IPR002273(Lutropin-choriogonadotropic hormone receptor)|interpro:IPR026906|interpro:IPR032675|rcsb pdb:1LUT|rcsb pdb:1XUL|reactome:R-HSA-375281|reactome:R-HSA-418555|reactome:R-HSA-9660821|interpro:IPR017452(GPCR, rhodopsin-like superfamily)	refseq:NP_000224.2|ensembl:ENSG00000138039(gene)|ensembl:ENST00000294954(transcript)|go:"GO:0001541"(ovarian follicle development)|go:"GO:0004964"(luteinizing hormone receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007189"(adenylate cyclase-activating G protein-coupled receptor signaling pathway)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007200"(phospholipase C-activating G protein-coupled receptor signaling pathway)|go:"GO:0008528"(G protein-coupled peptide receptor activity)|go:"GO:0008584"(male gonad development)|go:"GO:0009755"(hormone-mediated signaling pathway)|go:"GO:0022602"(ovulation cycle process)|go:"GO:0030539"(male genitalia development)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0035472"(choriogonadotropin hormone receptor activity)|go:"GO:0038106"(choriogonadotropin hormone binding)|go:"GO:0042700"(luteinizing hormone signaling pathway)|go:"GO:0050890"(cognition)|go:"GO:0071371"(cellular response to gonadotropin stimulus)|go:"GO:0071373"(cellular response to luteinizing hormone stimulus)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR002131(Glycoprotein hormone receptor)|interpro:IPR002273(Lutropin-choriogonadotropic hormone receptor)|interpro:IPR026906|interpro:IPR032675|rcsb pdb:1LUT|rcsb pdb:1XUL|reactome:R-HSA-375281|reactome:R-HSA-418555|reactome:R-HSA-9660821|interpro:IPR017452(GPCR, rhodopsin-like superfamily)	go:"GO:0005886"(plasma membrane)	-	-	figure legend:f3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/01/14	2014/10/16	rogid:YoSJGoLeUq4Oj+xCVrb9XhHUcBg9606	rogid:YoSJGoLeUq4Oj+xCVrb9XhHUcBg9606	intact-crc:00ED96DD63D571A8|rigid:/FjUdxUC5hJYQ/EWWZCh9xIVWs0	false	green fluorescent protein tag:?-?	mcherry fluorescent protein tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)