#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9108765|imex:IM-22095-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2b f2c f2d f2e|comment:"High molecular weight crosslinking products were efficiently generated for WT Ube2g2 and the Ube2g2 C75A mutant, but not for the C48A and C89A mutants (Fig 2C–E), suggesting that the crosslinked dimer/oligomers are formed between Cys48 and Cys89 of two neighboring Ube2g2s. Thus, it is likely that in a functional Ube2g2 dimer (see below), Cys48 in one Ube2g2 is in proximity to the catalytic Cys89 of another Ube2g2. This arrangement allows BMH to crosslink multiple Ube2g2 molecules into a higher order oligomer. To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:E0B22E064C89D666|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation disrupting interaction:48-48	mutation disrupting interaction:48-48	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9108802|imex:IM-22095-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2b f2c f2d f2e|comment:"High molecular weight crosslinking products were efficiently generated for WT Ube2g2 and the Ube2g2 C75A mutant, but not for the C48A and C89A mutants (Fig 2C–E), suggesting that the crosslinked dimer/oligomers are formed between Cys48 and Cys89 of two neighboring Ube2g2s. Thus, it is likely that in a functional Ube2g2 dimer (see below), Cys48 in one Ube2g2 is in proximity to the catalytic Cys89 of another Ube2g2. This arrangement allows BMH to crosslink multiple Ube2g2 molecules into a higher order oligomer. To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:6D2E6C17D067A177|rigid:eC053eOvJMII2Lp3oD3HdHb7ZP8	false	mutation disrupting interaction:89-89	mutation disrupting interaction:89-89	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109290|imex:IM-22095-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f4a f4b|comment:"The results revealed that several mutants generated fewer crosslinking products when compared to WT Ube2g2 (Fig 4A and B and data not shown). Among these mutants, two [referred to as M3 (D98R/D99R/M101R) and M4 (E129R/R148E/Y152E), respectively] were selected for further characterization because these mutations reduced Ube2g2 dimerization most significantly (Fig 4B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:02E6ED559AEE0606|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:129-129,148-148,152-152	mutation decreasing interaction:129-129,148-148,152-152	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109249|imex:IM-22095-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f4a f4b|comment:"The results revealed that several mutants generated fewer crosslinking products when compared to WT Ube2g2 (Fig 4A and B and data not shown). Among these mutants, two [referred to as M3 (D98R/D99R/M101R) and M4 (E129R/R148E/Y152E), respectively] were selected for further characterization because these mutations reduced Ube2g2 dimerization most significantly (Fig 4B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:62C585A60F15D424|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:98-98,99-99,101-101	mutation decreasing interaction:98-98,99-99,101-101	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9108786|imex:IM-22095-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2b f2c f2d f2e|comment:"High molecular weight crosslinking products were efficiently generated for WT Ube2g2 and the Ube2g2 C75A mutant, but not for the C48A and C89A mutants (Fig 2C–E), suggesting that the crosslinked dimer/oligomers are formed between Cys48 and Cys89 of two neighboring Ube2g2s. Thus, it is likely that in a functional Ube2g2 dimer (see below), Cys48 in one Ube2g2 is in proximity to the catalytic Cys89 of another Ube2g2. This arrangement allows BMH to crosslink multiple Ube2g2 molecules into a higher order oligomer. To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:163E519559C86D73|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation disrupting interaction:89-89	mutation disrupting interaction:89-89	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9108827|imex:IM-22095-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2b f2c f2d f2e|comment:"High molecular weight crosslinking products were efficiently generated for WT Ube2g2 and the Ube2g2 C75A mutant, but not for the C48A and C89A mutants (Fig 2C–E), suggesting that the crosslinked dimer/oligomers are formed between Cys48 and Cys89 of two neighboring Ube2g2s. Thus, it is likely that in a functional Ube2g2 dimer (see below), Cys48 in one Ube2g2 is in proximity to the catalytic Cys89 of another Ube2g2. This arrangement allows BMH to crosslink multiple Ube2g2 molecules into a higher order oligomer. To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:090F176538A91348|rigid:eC053eOvJMII2Lp3oD3HdHb7ZP8	false	mutation disrupting interaction:48-48	mutation disrupting interaction:48-48	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0969"(bio-layer interferometry)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109137|imex:IM-22095-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2f sf3a sf3b sf3c|comment:"To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity. Consistent with our hypothesis, WT Ube2g2 interacted with each other with a KD of around 8 μM, whereas the C48A mutant dimerized with a significantly reduced affinity (Fig 2F, supplementary Fig S3B and C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:8.1x10^-6(molar)	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:62E4D3F4ACB0FD71|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	-	-	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0969"(bio-layer interferometry)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109104|imex:IM-22095-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2f|comment:"To see if the C48A mutation affected Ube2g2 dimerization, we used Biolayer Interferometry to measure its dimerization activity. Consistent with our hypothesis, WT Ube2g2 interacted with each other with a KD of around 8 μM, whereas the C48A mutant dimerized with a significantly reduced affinity (Fig 2F, supplementary Fig S3B and C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:45.0x10^-6(molar)	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:5F3CF66E90912C98|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:48-48	mutation decreasing interaction:48-48	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0872"(atomic force microscopy)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109315|imex:IM-22095-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f4c sf3d-g|comment:"AFM experiments showed that the most probable rupture forces for the M3 and M4 mutants were significantly reduced comparing with WT Ube2g2 (Fig 4C and supplementary Fig S3D–G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:E1D6330C7DE3E080|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:129-129,148-148,152-152	mutation decreasing interaction:129-129,148-148,152-152	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0872"(atomic force microscopy)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109181|imex:IM-22095-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f2g f2h|comment:"In addition, atomic force microscopy (AFM) was used to directly measure the rupture force required to dissociate the E2 dimer for the Ube2g2 variants (Fig 2G). The results showed that the most probable rupture force between two WT Ube2g2 molecules was 24.08 ± 1.19 pN, while between two C48A mutants, it was 17.63 ± 0.56 pN (Fig 2H)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:3CAF662D4B72DFCD|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:48-48	mutation decreasing interaction:48-48	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P60605	uniprotkb:P60605	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	intact:EBI-9108745|uniprotkb:P56554|ensembl:ENSMUSP00000133515	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:ub2g2_mouse(display_long)|uniprotkb:Ube2g2(gene name)|psi-mi:Ube2g2(display_short)|uniprotkb:Ubiquitin carrier protein G2(gene name synonym)|uniprotkb:Ubiquitin-protein ligase G2(gene name synonym)|uniprotkb:Ubc7(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme G2(gene name synonym)	psi-mi:"MI:0872"(atomic force microscopy)	Liu et al. (2014)	pubmed:24366945|imex:IM-22095	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9109345|imex:IM-22095-12	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	refseq:NP_062777.2|dip:DIP-29061N|ensembl:ENSMUSG00000009293(gene)|ensembl:ENSMUST00000174510(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005811"(lipid droplet)|go:"GO:0005829"(cytosol)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0030433"(ubiquitin-dependent ERAD pathway)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042802"(identical protein binding)|go:"GO:0044257"(cellular protein catabolic process)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070936"(protein K48-linked ubiquitination)|go:"GO:1904153"(negative regulation of retrograde protein transport, ER to cytosol)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|rcsb pdb:3FSH|reactome:R-MMU-8866652|reactome:R-MMU-983168|go:"GO:0000209"(protein polyubiquitination)	-	-	-	figure legend:f4c sf3d-g|comment:"AFM experiments showed that the most probable rupture forces for the M3 and M4 mutants were significantly reduced comparing with WT Ube2g2 (Fig 4C and supplementary Fig S3D–G)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/03	2014/10/16	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	rogid:Q/uslqX/WH/SxKEPYeMmU9CjTBI10090	intact-crc:88A6EF6F8684F813|rigid:2CDLmFs2XiQtHNBy4RX55eUNggA	false	mutation decreasing interaction:99-99,98-98,101-101	mutation decreasing interaction:99-99,98-98,101-101	2	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)