#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P53220	uniprotkb:Q02776	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202394|imex:IM-22956-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:FC140F3034E5B5BB	crc64:778D54F5C4ED27C8	figure legend:f2b f2c f2f|comment:"Purified proteins were mixed in  equimolar amounts and subjected to crosslinking prior to  SDS–polyacrylamide gel electrophoresis (PAGE) analyses.  Tim21IMS formed a dimer upon Cu2þ-induced crosslinking,  while Tim21IMS-C128S did not, due to the absence of the  cysteine residue (Figure 2B, lanes 4 versus 5). Similarly,  Tim50IMS formed dimers upon crosslinking (Figure 2B,  lane 6),"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:4766A529364D9859|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	binding-associated region:103-225	binding-associated region:132-476	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P32897	uniprotkb:Q02776	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206896|imex:IM-22956-13	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:08E5AB4F400F675B	crc64:778D54F5C4ED27C8	figure legend:sf2b|comment:"Indeed, a crosslinking-adduct was formed between Tim50IMS  and Tim23IMS, but not with Tim23IMS YL70AA in an in vitro  chemical crosslinking experiment (Supplementary Figure  S2B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:7DC631515839F1B8|rigid:5puIeU6SmlwUYZS5th4V8JbuIRs	false	mutation disrupting interaction:70-71	binding-associated region:132-476	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q02776	uniprotkb:P04037	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-5039|uniprotkb:A2TBN6|uniprotkb:D6VTW7|ensemblfungi:YGL187C	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:cox4_yeast(display_long)|uniprotkb:COX4(gene name)|psi-mi:COX4(display_short)|uniprotkb:YGL187C(locus name)|uniprotkb:G1362(orf name)|uniprotkb:Cytochrome c oxidase polypeptide IV(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206867|imex:IM-22956-12	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	refseq:NP_011328.1|sgd:S000003155|dip:DIP-2763N|mint:P04037|ensemblfungi:YGL187C(gene)|ensemblfungi:YGL187C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005751"(mitochondrial respiratory chain complex IV)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0006123"(mitochondrial electron transport, cytochrome c to oxygen)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016491"(oxidoreductase activity)|go:"GO:0033617"(mitochondrial cytochrome c oxidase assembly)|go:"GO:1902600"(proton transmembrane transport)|interpro:IPR002124(Cytochrome c oxidase, subunit Vb)|interpro:IPR020893|interpro:IPR036972|rcsb pdb:1HR8|rcsb pdb:2ODX|rcsb pdb:6GIQ|rcsb pdb:6HU9|rcsb pdb:6T0B|rcsb pdb:6T15|rcsb pdb:6YMX|rcsb pdb:6YMY	-	crc64:778D54F5C4ED27C8	-	figure legend:sf1c|comment:"Similarly, while Tim50IMS could be efficiently crosslinked to  Cox4 presequence peptides with the homo-bifunctional  chemical crosslinker 1,5-Difluoro-2,4-dinitrobenzene  (DFDNB), Tim50DPBD and Tim50C displayed no significant  presequence crosslinks (Supplementary Figure S1C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:BgFxxftKsM1hXTeVpOGK5ILmmvY559292	intact-crc:4419E22CA0CFEDE8|rigid:Epo1lzZjLrVHyKGyRyHS+OCWfis	false	binding-associated region:132-476	binding-associated region:1-25	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0083"(peptide synthesis)
uniprotkb:P53220	uniprotkb:P53220	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202399|imex:IM-22956-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	-	crc64:FC140F3034E5B5BB	crc64:FC140F3034E5B5BB	figure legend:f2b f2c f2f|comment:"Purified proteins were mixed in  equimolar amounts and subjected to crosslinking prior to  SDS–polyacrylamide gel electrophoresis (PAGE) analyses.  Tim21IMS formed a dimer upon Cu2þ-induced crosslinking,  while Tim21IMS-C128S did not, due to the absence of the  cysteine residue (Figure 2B, lanes 4 versus 5). Similarly,  Tim50IMS formed dimers upon crosslinking (Figure 2B,  lane 6),"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:E211D4D742C2D5EE|rigid:9J/CUZtv6H4R3uRFHtNYSvPf5NE	false	binding-associated region:103-225|mutation disrupting interaction:128-128	binding-associated region:103-225|mutation disrupting interaction:128-128	2	0	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P53220	uniprotkb:Q02776	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202437|imex:IM-22956-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:FC140F3034E5B5BB	crc64:778D54F5C4ED27C8	figure legend:f2f sf1a|comment:"Purified proteins were mixed in  equimolar amounts and subjected to crosslinking prior to  SDS–polyacrylamide gel electrophoresis (PAGE) analyses.  Tim21IMS formed a dimer upon Cu2þ-induced crosslinking,  while Tim21IMS-C128S did not, due to the absence of the  cysteine residue (Figure 2B, lanes 4 versus 5). Similarly,  Tim50IMS formed dimers upon crosslinking (Figure 2B,  lane 6),"|comment:"We purified Tim21IMS and Tim50C  (amino acids 164–361), corresponding to the crystallized  portion of Tim50, and addressed protein interactions  in vitro. Similar to Tim50IMS, Tim50C was able to dimerize  and to hetero-oligomerize with Tim21IMS (Supplementary  Figure S1A)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:6C662B9541335E91|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	binding-associated region:103-225	binding-associated region:164-361	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q02776	uniprotkb:Q02776	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0030"(cross-linking study)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202414|imex:IM-22956-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:778D54F5C4ED27C8	crc64:778D54F5C4ED27C8	figure legend:f2b|comment:"Purified proteins were mixed in  equimolar amounts and subjected to crosslinking prior to  SDS–polyacrylamide gel electrophoresis (PAGE) analyses.  Tim21IMS formed a dimer upon Cu2þ-induced crosslinking,  while Tim21IMS-C128S did not, due to the absence of the  cysteine residue (Figure 2B, lanes 4 versus 5). Similarly,  Tim50IMS formed dimers upon crosslinking (Figure 2B,  lane 6),"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:ED65F9D3C3609F86|rigid:I5NplpSL+jEaUJ2e5GzPMaz7KoE	false	binding-associated region:132-476	binding-associated region:132-476	2	0	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P11884	uniprotkb:Q02776	intact:EBI-916402|uniprotkb:Q6Q288|uniprotkb:Q6Q289|uniprotkb:Q6Q290|uniprotkb:Q8K3V8|uniprotkb:Q91ZD7	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:aldh2_rat(display_long)|uniprotkb:ALDH class 2(gene name synonym)|uniprotkb:ALDH1(gene name synonym)|uniprotkb:ALDH-E2(gene name synonym)|uniprotkb:Aldh2(gene name)|psi-mi:Aldh2(display_short)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202491|imex:IM-22956-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032570"(response to progesterone)|go:"GO:0032870"(cellular response to hormone stimulus)|go:"GO:0033574"(response to testosterone)|go:"GO:0035094"(response to nicotine)|go:"GO:0042802"(identical protein binding)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043878"("glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity")|go:"GO:0045471"(response to ethanol)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0050727"(regulation of inflammatory response)|go:"GO:0055093"(response to hyperoxia)|go:"GO:0070404"(NADH binding)|go:"GO:0071398"(cellular response to fatty acid)|go:"GO:0110095"(cellular detoxification of aldehyde)|go:"GO:1902882"(regulation of response to oxidative stress)|go:"GO:1904639"(cellular response to resveratrol)|go:"GO:2000377"(regulation of reactive oxygen species metabolic process)|go:"GO:0001889"(liver development)|go:"GO:0002931"(response to ischemia)|go:"GO:0004029"("aldehyde dehydrogenase (NAD+) activity")|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0006067"(ethanol metabolic process)|go:"GO:0006068"(ethanol catabolic process)|go:"GO:0006117"(acetaldehyde metabolic process)|go:"GO:0008631"(intrinsic apoptotic signaling pathway in response to oxidative stress)|go:"GO:0008637"(apoptotic mitochondrial changes)|go:"GO:0009408"(response to heat)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0032355"(response to estradiol)|rcsb pdb:5AZ8|rgd:69219|refseq:NP_115792.1|interpro:IPR016161(Aldehyde/histidinol dehydrogenase)|interpro:IPR016160(Aldehyde dehydrogenase, conserved site)|interpro:IPR016162(Aldehyde dehydrogenase, N-terminal)|interpro:IPR015590(Aldehyde dehydrogenase)|rcsb pdb:1OM2|rcsb pdb:2V1S|rcsb pdb:2V1T|rcsb pdb:3AWR|rcsb pdb:3AX2|rcsb pdb:3AX3|rcsb pdb:3AX5|interpro:IPR029510|interpro:IPR016163(Aldehyde dehydrogenase, C-terminal)|reactome:R-RNO-71384|reactome:R-RNO-380612	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	-	crc64:778D54F5C4ED27C8	figure legend:t1 f3e|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:3.94x10^-6	2014/02/12	2014/10/16	rogid:ky5VO6/2tobrmxxftl2k8kWifkA10116	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:62D1D0FE21C98E5B|rigid:nHJzcr8IcR6YpZc4ef+D9C/K4EA	false	binding-associated region:1-22	binding-associated region:164-361	-	-	psi-mi:"MI:0083"(peptide synthesis)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P53220	uniprotkb:Q02776	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202458|imex:IM-22956-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:FC140F3034E5B5BB	crc64:778D54F5C4ED27C8	figure legend:f2g t1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:0.093x10^-6	2014/02/12	2014/10/16	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:5733055A3F001F77|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	binding-associated region:103-225	binding-associated region:164-361	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P32897	uniprotkb:P53220	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202557|imex:IM-22956-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	-	crc64:08E5AB4F400F675B	crc64:FC140F3034E5B5BB	figure legend:t1 sf2a sf2c|comment:"Therefore, we analysed the binding affinity of  Tim21IMS to immobilized Tim23IMS in an in vitro system via  SPR (Supplementary Figure S2A). Our results confirmed their  direct interaction with an KD of B1 mM (Table I), suggesting  that Tim23 could exert its coordinating effect on Tim21–  Tim50 complex formation via its direct interaction with  both proteins."|comment:"At the same time, SPR analysis showed that the  mutant’s interaction with Tim21 was not affected (Supplementary  Figure S2C)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.0x10^-6	2014/02/12	2014/10/16	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:96F0D8C58281B8DA|rigid:H49cYaZsQNqJdC1/1Cw5YJWyDOA	false	-	binding-associated region:103-225	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q02776	uniprotkb:P11884	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-916402|uniprotkb:Q6Q288|uniprotkb:Q6Q289|uniprotkb:Q6Q290|uniprotkb:Q8K3V8|uniprotkb:Q91ZD7	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:aldh2_rat(display_long)|uniprotkb:ALDH class 2(gene name synonym)|uniprotkb:ALDH1(gene name synonym)|uniprotkb:ALDH-E2(gene name synonym)|uniprotkb:Aldh2(gene name)|psi-mi:Aldh2(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202541|imex:IM-22956-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032570"(response to progesterone)|go:"GO:0032870"(cellular response to hormone stimulus)|go:"GO:0033574"(response to testosterone)|go:"GO:0035094"(response to nicotine)|go:"GO:0042802"(identical protein binding)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043878"("glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity")|go:"GO:0045471"(response to ethanol)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0050727"(regulation of inflammatory response)|go:"GO:0055093"(response to hyperoxia)|go:"GO:0070404"(NADH binding)|go:"GO:0071398"(cellular response to fatty acid)|go:"GO:0110095"(cellular detoxification of aldehyde)|go:"GO:1902882"(regulation of response to oxidative stress)|go:"GO:1904639"(cellular response to resveratrol)|go:"GO:2000377"(regulation of reactive oxygen species metabolic process)|go:"GO:0001889"(liver development)|go:"GO:0002931"(response to ischemia)|go:"GO:0004029"("aldehyde dehydrogenase (NAD+) activity")|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0006067"(ethanol metabolic process)|go:"GO:0006068"(ethanol catabolic process)|go:"GO:0006117"(acetaldehyde metabolic process)|go:"GO:0008631"(intrinsic apoptotic signaling pathway in response to oxidative stress)|go:"GO:0008637"(apoptotic mitochondrial changes)|go:"GO:0009408"(response to heat)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0032355"(response to estradiol)|rcsb pdb:5AZ8|rgd:69219|refseq:NP_115792.1|interpro:IPR016161(Aldehyde/histidinol dehydrogenase)|interpro:IPR016160(Aldehyde dehydrogenase, conserved site)|interpro:IPR016162(Aldehyde dehydrogenase, N-terminal)|interpro:IPR015590(Aldehyde dehydrogenase)|rcsb pdb:1OM2|rcsb pdb:2V1S|rcsb pdb:2V1T|rcsb pdb:3AWR|rcsb pdb:3AX2|rcsb pdb:3AX3|rcsb pdb:3AX5|interpro:IPR029510|interpro:IPR016163(Aldehyde dehydrogenase, C-terminal)|reactome:R-RNO-71384|reactome:R-RNO-380612	-	crc64:778D54F5C4ED27C8	-	figure legend:t1 F3C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:6.94x10^-6	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:ky5VO6/2tobrmxxftl2k8kWifkA10116	intact-crc:CA2F3FBA499D5059|rigid:nHJzcr8IcR6YpZc4ef+D9C/K4EA	false	binding-associated region:395-476	binding-associated region:1-22	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0083"(peptide synthesis)
uniprotkb:P53220	uniprotkb:Q02776	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202446|imex:IM-22956-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:FC140F3034E5B5BB	crc64:778D54F5C4ED27C8	figure legend:f2d t1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:0.26x10^-6	2014/02/12	2014/10/16	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:616103900333BC59|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	binding-associated region:103-225	binding-associated region:132-476	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P11884	uniprotkb:Q02776	intact:EBI-916402|uniprotkb:Q6Q288|uniprotkb:Q6Q289|uniprotkb:Q6Q290|uniprotkb:Q8K3V8|uniprotkb:Q91ZD7	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:aldh2_rat(display_long)|uniprotkb:ALDH class 2(gene name synonym)|uniprotkb:ALDH1(gene name synonym)|uniprotkb:ALDH-E2(gene name synonym)|uniprotkb:Aldh2(gene name)|psi-mi:Aldh2(display_short)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0107"(surface plasmon resonance)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202507|imex:IM-22956-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032570"(response to progesterone)|go:"GO:0032870"(cellular response to hormone stimulus)|go:"GO:0033574"(response to testosterone)|go:"GO:0035094"(response to nicotine)|go:"GO:0042802"(identical protein binding)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043878"("glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity")|go:"GO:0045471"(response to ethanol)|go:"GO:0048149"(behavioral response to ethanol)|go:"GO:0050727"(regulation of inflammatory response)|go:"GO:0055093"(response to hyperoxia)|go:"GO:0070404"(NADH binding)|go:"GO:0071398"(cellular response to fatty acid)|go:"GO:0110095"(cellular detoxification of aldehyde)|go:"GO:1902882"(regulation of response to oxidative stress)|go:"GO:1904639"(cellular response to resveratrol)|go:"GO:2000377"(regulation of reactive oxygen species metabolic process)|go:"GO:0001889"(liver development)|go:"GO:0002931"(response to ischemia)|go:"GO:0004029"("aldehyde dehydrogenase (NAD+) activity")|go:"GO:0005739"(mitochondrion)|go:"GO:0005759"(mitochondrial matrix)|go:"GO:0006067"(ethanol metabolic process)|go:"GO:0006068"(ethanol catabolic process)|go:"GO:0006117"(acetaldehyde metabolic process)|go:"GO:0008631"(intrinsic apoptotic signaling pathway in response to oxidative stress)|go:"GO:0008637"(apoptotic mitochondrial changes)|go:"GO:0009408"(response to heat)|go:"GO:0014070"(response to organic cyclic compound)|go:"GO:0032355"(response to estradiol)|rcsb pdb:5AZ8|rgd:69219|refseq:NP_115792.1|interpro:IPR016161(Aldehyde/histidinol dehydrogenase)|interpro:IPR016160(Aldehyde dehydrogenase, conserved site)|interpro:IPR016162(Aldehyde dehydrogenase, N-terminal)|interpro:IPR015590(Aldehyde dehydrogenase)|rcsb pdb:1OM2|rcsb pdb:2V1S|rcsb pdb:2V1T|rcsb pdb:3AWR|rcsb pdb:3AX2|rcsb pdb:3AX3|rcsb pdb:3AX5|interpro:IPR029510|interpro:IPR016163(Aldehyde dehydrogenase, C-terminal)|reactome:R-RNO-71384|reactome:R-RNO-380612	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	-	crc64:778D54F5C4ED27C8	figure legend:t1 f3a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:3.79x10^-6	2014/02/12	2014/10/16	rogid:ky5VO6/2tobrmxxftl2k8kWifkA10116	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:548516341F2A2D75|rigid:nHJzcr8IcR6YpZc4ef+D9C/K4EA	false	binding-associated region:1-22	binding-associated region:132-476	-	-	psi-mi:"MI:0083"(peptide synthesis)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q02776	uniprotkb:P53220	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0096"(pull down)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9202643|imex:IM-22956-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	-	crc64:778D54F5C4ED27C8	crc64:FC140F3034E5B5BB	figure legend:f5g|comment:"To address the effect of Tim23IMS on Tim21–Tim50 by an  alternative approach, we performed pull-down experiments.  Therefore, purified Tim50IMS was added to Tim21IMS immobilized  on Sepharose beads in the presence of increasing  concentrations of Tim23IMS. Addition of wild-type Tim23IMS  significantly increased the binding of Tim50IMS to the  Tim21 beads, whereas Tim23IMS YL70AA did not promote this  binding (Figure 5G and H)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:76D2555489FD222B|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	binding-associated region:132-476	binding-associated region:103-225	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q02776	uniprotkb:P53220	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206951|imex:IM-22956-16	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	go:"GO:0005739"(mitochondrion)	crc64:778D54F5C4ED27C8	crc64:FC140F3034E5B5BB	figure legend:sf2e|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:8ADFF35A74664A4E|rigid:B7jsha5t+Y1yV79iYxnWRCcTqj4	false	ha tag:?-?|sufficient binding region:1-361	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02776	uniprotkb:P32897	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206939|imex:IM-22956-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	-	crc64:778D54F5C4ED27C8	crc64:08E5AB4F400F675B	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	intact-crc:ADC9D529769B5343|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02776	uniprotkb:P53220	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206939|imex:IM-22956-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	-	crc64:778D54F5C4ED27C8	crc64:FC140F3034E5B5BB	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:ADC9D529769B5343|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02776	uniprotkb:P36147	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	intact:EBI-26467|uniprotkb:D6VXC6|ensemblfungi:YKR065C	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:pam17_yeast(display_long)|uniprotkb:YKR065C(locus name)|uniprotkb:PAM17(gene name)|psi-mi:PAM17(display_short)|uniprotkb:FMP18(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206939|imex:IM-22956-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	sgd:S000001773|refseq:NP_012991.3|mint:P36147|dip:DIP-2128N|ensemblfungi:YKR065C(gene)|ensemblfungi:YKR065C_mRNA(transcript)|go:"GO:0001405"(PAM complex, Tim23 associated import motor)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013875(Mitochondrial import protein Pam17)	-	crc64:778D54F5C4ED27C8	-	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	rogid:Vgo0cGQ1tciZxf3wV05QG79I4mg559292	intact-crc:ADC9D529769B5343|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P32897	uniprotkb:P53220	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	intact:EBI-23128|uniprotkb:D6VUG9|uniprotkb:Q45U51|ensemblfungi:YGR033C	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:tim21_yeast(display_long)|uniprotkb:TIM21(gene name)|psi-mi:TIM21(display_short)|uniprotkb:YGR033C(locus name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206945|imex:IM-22956-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	sgd:S000003265|refseq:NP_011547.3|dip:DIP-3940N|mint:P53220|ensemblfungi:YGR033C(gene)|ensemblfungi:YGR033C_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013261(Mitochondrial import inner membrane translocase, subunit Tim21)|interpro:IPR038552|rcsb pdb:2CIU|rcsb pdb:2MF7|rcsb pdb:6K7D|rcsb pdb:6K7E|rcsb pdb:6K7F|rcsb pdb:6K8Q	-	crc64:08E5AB4F400F675B	crc64:FC140F3034E5B5BB	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	rogid:abC8uNkog3dd+hn3zP8hlL64p7A559292	intact-crc:5147B3E49B2EC65A|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P32897	uniprotkb:Q02776	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	intact:EBI-30302|uniprotkb:D6W3V2|ensemblfungi:YPL063W	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:tim50_yeast(display_long)|uniprotkb:TIM50(gene name)|psi-mi:TIM50(display_short)|uniprotkb:YPL063W(locus name)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206945|imex:IM-22956-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	refseq:NP_015262.1|sgd:S000005984|dip:DIP-8544N|mint:Q02776|ensemblfungi:YPL063W(gene)|ensemblfungi:YPL063W_mRNA(transcript)|go:"GO:0004721"(phosphoprotein phosphatase activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046902"(regulation of mitochondrial membrane permeability)|interpro:IPR004274(NLI interacting factor)|interpro:IPR023214|interpro:IPR027111|interpro:IPR036412|rcsb pdb:4QQF	-	crc64:08E5AB4F400F675B	crc64:778D54F5C4ED27C8	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	rogid:mNxn2HKrmn98XCcUeDj/OXUbwAM559292	intact-crc:5147B3E49B2EC65A|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P32897	uniprotkb:P36147	intact:EBI-9136|ensemblfungi:YNR017W|uniprotkb:D6W1J2	intact:EBI-26467|uniprotkb:D6VXC6|ensemblfungi:YKR065C	psi-mi:tim23_yeast(display_long)|uniprotkb:MIM23(gene name synonym)|uniprotkb:MPI3(gene name synonym)|uniprotkb:N3180(orf name)|uniprotkb:YNR017W(locus name)|uniprotkb:TIM23(gene name)|psi-mi:TIM23(display_short)|uniprotkb:MAS6(gene name synonym)|uniprotkb:Mitochondrial protein import protein 3(gene name synonym)|uniprotkb:Mitochondrial protein import protein MAS6(gene name synonym)|uniprotkb:Membrane import machinery protein MIM23(gene name synonym)	psi-mi:pam17_yeast(display_long)|uniprotkb:YKR065C(locus name)|uniprotkb:PAM17(gene name)|psi-mi:PAM17(display_short)|uniprotkb:FMP18(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lytovchenko et al. (2013)	imex:IM-22956|pubmed:23403928	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9206945|imex:IM-22956-15	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-SCE-1268020|ensemblfungi:YNR017W(gene)|ensemblfungi:YNR017W_mRNA(transcript)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0005744"(TIM23 mitochondrial import inner membrane translocase complex)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0030150"(protein import into mitochondrial matrix)|go:"GO:0030943"(mitochondrion targeting sequence binding)|go:"GO:0031305"(integral component of mitochondrial inner membrane)|interpro:IPR005681(Mitochondrial import inner membrane translocase, subunit Tim23)|refseq:NP_014414.3|sgd:S000005300|dip:DIP-2451N|mint:P32897	sgd:S000001773|refseq:NP_012991.3|mint:P36147|dip:DIP-2128N|ensemblfungi:YKR065C(gene)|ensemblfungi:YKR065C_mRNA(transcript)|go:"GO:0001405"(PAM complex, Tim23 associated import motor)|go:"GO:0005739"(mitochondrion)|go:"GO:0005743"(mitochondrial inner membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030150"(protein import into mitochondrial matrix)|interpro:IPR013875(Mitochondrial import protein Pam17)	-	crc64:08E5AB4F400F675B	-	figure legend:f6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/02/12	2014/10/16	rogid:nA6mnSq0Ek0I5g8LSJpLjojiRvc559292	rogid:Vgo0cGQ1tciZxf3wV05QG79I4mg559292	intact-crc:5147B3E49B2EC65A|rigid:HOD1SEVLLazFhUPx385ATxUoLT8	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)