#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P41542 uniprotkb:Q63714 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7588697|intact:MINT-4599147 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) - - comment:mint figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62931 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35152 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62896 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q4KM74 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62839 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35165 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q5EGY4 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q08851 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62991 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-918383|uniprotkb:Q62843|intact:EBI-11702597|ensembl:ENSRNOP00000039132 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:scfd1_rat(display_long)|uniprotkb:Syntaxin-binding protein 1-like 2(gene name synonym)|uniprotkb:Vesicle transport-related protein Ra410(gene name synonym)|uniprotkb:SLY1 homolog(gene name synonym)|uniprotkb:Scfd1(gene name)|psi-mi:Scfd1(display_short)|uniprotkb:Ra410(gene name synonym)|uniprotkb:Sly1(gene name synonym)|uniprotkb:Stxbp1l2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997628|imex:IM-23550-18 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:619828|refseq:NP_062237.1|ensembl:ENSRNOG00000031203(gene)|ensembl:ENSRNOT00000040548(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005798"(Golgi-associated vesicle)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0009636"(response to toxic substance)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051223"(regulation of protein transport)|go:"GO:0060628"(regulation of ER to Golgi vesicle-mediated transport)|go:"GO:1901998"(toxin transport)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001619(Sec1-like protein)|interpro:IPR027482|interpro:IPR036045|interpro:IPR043127|interpro:IPR043154|rcsb pdb:1Y9J|reactome:R-RNO-204005|reactome:R-RNO-8980692 - - - figure legend:Fig. 1c|comment:Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue.|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Zx6Xz6WSQHh9ZCPoD3YpL7FracI10116 intact-crc:AE65821E8664E257|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false biotin tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62931 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62896 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35165 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q4KM74 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q5EGY4 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35152 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997818|imex:IM-23550-20 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:B24A00DB43C43B62|rigid:r+VhAwDFr+so7mMrIEW2m73A1AE false sufficient binding region:843-930|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62931 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62896 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q5EGY4 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35152 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62991 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-918383|uniprotkb:Q62843|intact:EBI-11702597|ensembl:ENSRNOP00000039132 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:scfd1_rat(display_long)|uniprotkb:Syntaxin-binding protein 1-like 2(gene name synonym)|uniprotkb:Vesicle transport-related protein Ra410(gene name synonym)|uniprotkb:SLY1 homolog(gene name synonym)|uniprotkb:Scfd1(gene name)|psi-mi:Scfd1(display_short)|uniprotkb:Ra410(gene name synonym)|uniprotkb:Sly1(gene name synonym)|uniprotkb:Stxbp1l2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:619828|refseq:NP_062237.1|ensembl:ENSRNOG00000031203(gene)|ensembl:ENSRNOT00000040548(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005798"(Golgi-associated vesicle)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0009636"(response to toxic substance)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051223"(regulation of protein transport)|go:"GO:0060628"(regulation of ER to Golgi vesicle-mediated transport)|go:"GO:1901998"(toxin transport)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001619(Sec1-like protein)|interpro:IPR027482|interpro:IPR036045|interpro:IPR043127|interpro:IPR043154|rcsb pdb:1Y9J|reactome:R-RNO-204005|reactome:R-RNO-8980692 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Zx6Xz6WSQHh9ZCPoD3YpL7FracI10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62839 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q08851 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q4KM74 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35165 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998006|imex:IM-23550-22 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:3ED3B98F7F301D19|rigid:yEuzJ2g6N/6mR9IrQ1nACrwOq5o false his tag:?-?|sufficient binding region:637-930 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62931 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q4KM74 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35165 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q08851 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62896 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q5EGY4 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35152 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62991 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-918383|uniprotkb:Q62843|intact:EBI-11702597|ensembl:ENSRNOP00000039132 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:scfd1_rat(display_long)|uniprotkb:Syntaxin-binding protein 1-like 2(gene name synonym)|uniprotkb:Vesicle transport-related protein Ra410(gene name synonym)|uniprotkb:SLY1 homolog(gene name synonym)|uniprotkb:Scfd1(gene name)|psi-mi:Scfd1(display_short)|uniprotkb:Ra410(gene name synonym)|uniprotkb:Sly1(gene name synonym)|uniprotkb:Stxbp1l2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997789|imex:IM-23550-19 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:619828|refseq:NP_062237.1|ensembl:ENSRNOG00000031203(gene)|ensembl:ENSRNOT00000040548(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005798"(Golgi-associated vesicle)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0009636"(response to toxic substance)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051223"(regulation of protein transport)|go:"GO:0060628"(regulation of ER to Golgi vesicle-mediated transport)|go:"GO:1901998"(toxin transport)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001619(Sec1-like protein)|interpro:IPR027482|interpro:IPR036045|interpro:IPR043127|interpro:IPR043154|rcsb pdb:1Y9J|reactome:R-RNO-204005|reactome:R-RNO-8980692 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were significantly weaker than with the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Zx6Xz6WSQHh9ZCPoD3YpL7FracI10116 intact-crc:89474B61C4C9D31C|rigid:uUiLX9tRZkSEQMZaZim99sgo2xU false sufficient binding region:637-699|biotin tag:n-n - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q63714 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7588697|intact:MINT-4599147 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) - - comment:mint figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62931 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62839 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35165 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q4KM74 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q08851 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:O35152 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62896 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q5EGY4 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q62991 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-918383|uniprotkb:Q62843|intact:EBI-11702597|ensembl:ENSRNOP00000039132 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:scfd1_rat(display_long)|uniprotkb:Syntaxin-binding protein 1-like 2(gene name synonym)|uniprotkb:Vesicle transport-related protein Ra410(gene name synonym)|uniprotkb:SLY1 homolog(gene name synonym)|uniprotkb:Scfd1(gene name)|psi-mi:Scfd1(display_short)|uniprotkb:Ra410(gene name synonym)|uniprotkb:Sly1(gene name synonym)|uniprotkb:Stxbp1l2(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9997964|imex:IM-23550-21 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:619828|refseq:NP_062237.1|ensembl:ENSRNOG00000031203(gene)|ensembl:ENSRNOT00000040548(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000902"(cell morphogenesis)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005798"(Golgi-associated vesicle)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0009636"(response to toxic substance)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051223"(regulation of protein transport)|go:"GO:0060628"(regulation of ER to Golgi vesicle-mediated transport)|go:"GO:1901998"(toxin transport)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001619(Sec1-like protein)|interpro:IPR027482|interpro:IPR036045|interpro:IPR043127|interpro:IPR043154|rcsb pdb:1Y9J|reactome:R-RNO-204005|reactome:R-RNO-8980692 - - - figure legend:Fig. 1c|comment:"Instead of protein rSec22p the mammalian Sec22b was used as the former is the yeast homologue. Bet1 and GS15 (Bet1l) bands were weaker than for the FL p115 (Uso1) pulldown."|comment:"In addition to the Western Blot a silver staining was also performed on another SDS-PAGE gel (Fig. 1b) but only p115, giantin and GM130 were identified by weight (in p115 pulldown)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2015/11/10 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:Zx6Xz6WSQHh9ZCPoD3YpL7FracI10116 intact-crc:C1CC8EC8D2B1CB04|rigid:8SUh9lBXpgdDO/660pcbTzG1pXQ false sufficient binding region:637-959|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q5EGY4 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35165 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q4KM74 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62896 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35152 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020785|imex:IM-23550-9 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:"Fig. 2f (lane 4)"|agonist:"p115 (Uso1, P41542)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:93C031DF2E5E5754|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q63714 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-7588697|intact:MINT-4599147 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport comment:mint figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:O35152 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62839 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q5EGY4 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62896 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:P41542 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998106|imex:IM-23550-24 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lanes 3-6), 2c"|comment:When p115 is included, the SNARE bands are stronger and Giantin only starts to bind at 20 nM p115.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:FB02EB55C5D3E533|rigid:LF0saHNgN35sAT4ukWD7kWIuCR8 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35152 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62896 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q4KM74 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35165 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q5EGY4 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020800|imex:IM-23550-10 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 2f (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:B536926C279D54D7|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:O35152 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020646|imex:IM-23550-8 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 4)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|agonist:"p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:F916EA07648E6A19|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q5EGY4 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020646|imex:IM-23550-8 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 4)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|agonist:"p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:F916EA07648E6A19|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62896 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020646|imex:IM-23550-8 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 4)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|agonist:"p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:F916EA07648E6A19|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020646|imex:IM-23550-8 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 4)"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|agonist:"p115 (Uso1, P41542)"|comment:"The combination of adding full length p115 and its CC1 domain peptide results in weaker bands of all SNAREs and a lack of detection of p115 by WB while p115 on its own enhances the SNARE interactions (see EBI-10020539)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:F916EA07648E6A19|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62896 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020539|imex:IM-23550-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:530B674EA86D00F5|rigid:0lBa58GW4BYBXKTPt8O7yYl6f7Y false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:P41542 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020539|imex:IM-23550-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:530B674EA86D00F5|rigid:0lBa58GW4BYBXKTPt8O7yYl6f7Y false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020539|imex:IM-23550-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:530B674EA86D00F5|rigid:0lBa58GW4BYBXKTPt8O7yYl6f7Y false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:O35152 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020539|imex:IM-23550-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:530B674EA86D00F5|rigid:0lBa58GW4BYBXKTPt8O7yYl6f7Y false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q5EGY4 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10020539|imex:IM-23550-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lanes 3, 5-7)"|comment:"The addition of CC2 domain (728-765) of p115 (Uso1, P41542), C-terminal domain (521-595) of p115 (Uso1, P41542) or N-terminal domain (1-73) of GM130 (Golga2, Q62839) had not effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:530B674EA86D00F5|rigid:0lBa58GW4BYBXKTPt8O7yYl6f7Y false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q5EGY4 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998188|imex:IM-23550-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:FC69B3BE4DB3DC58|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:O35152 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998188|imex:IM-23550-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:FC69B3BE4DB3DC58|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62896 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998188|imex:IM-23550-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:FC69B3BE4DB3DC58|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998188|imex:IM-23550-27 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2e (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:FC69B3BE4DB3DC58|rigid:vRnxuY1xDM4fU5NXbJt0RZvrq2k false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q9JI51 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7573650|intact:MINT-5225883|uniprotkb:Q9JI52 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:vti1a_rat(display_long)|uniprotkb:Vti1a(gene name)|psi-mi:Vti1a(display_short)|uniprotkb:Vti1l2(gene name synonym)|uniprotkb:Vesicle transport v-SNARE protein Vti1-like 2(gene name synonym)|uniprotkb:Vti1-rp2(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 mint:Q9JI51|interpro:IPR027027|interpro:IPR007705(Vesicle transport v-SNARE)|interpro:IPR010989(t-SNARE)|refseq:NP_075589.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:621490|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005776"(autophagosome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006914"(autophagy)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016189"(synaptic vesicle to endosome fusion)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030665"(clathrin-coated vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0044306"(neuron projection terminus)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050882"(voluntary musculoskeletal movement)|go:"GO:0090161"(Golgi ribbon formation)|rcsb pdb:2NPS|reactome:R-RNO-6811438|reactome:R-RNO-6811440|interpro:IPR038407 - - comment:mint|function:V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be required for a fusion step during synaptic vesicle Recycling in the nerve terminal comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:MOylCkFQJsOJ8a/WTTU197k386I10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q63714 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7588697|intact:MINT-4599147 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) - - comment:mint comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35165 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q4KM74 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62896 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35152 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q5EGY4 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62839 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:P41542 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998136|imex:IM-23550-25 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - comment:When p115 is included, the SNARE bands are stronger. Vti1a bands are very faint.|figure legend:"Fig. 2b (lanes 3-6), 2d"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:BC99A14A7B7FD4F1|rigid:4vS+8wzAj5nfOhZOcL6fbRMIbRg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62839 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998096|imex:IM-23550-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lane 2), 2c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:1F0F3EF230F2538C|rigid:hclmfUmivnQg9W2z8Lpp4f6Ghac false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998096|imex:IM-23550-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lane 2), 2c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:1F0F3EF230F2538C|rigid:hclmfUmivnQg9W2z8Lpp4f6Ghac false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q5EGY4 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998096|imex:IM-23550-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lane 2), 2c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:1F0F3EF230F2538C|rigid:hclmfUmivnQg9W2z8Lpp4f6Ghac false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q62896 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998096|imex:IM-23550-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lane 2), 2c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:1F0F3EF230F2538C|rigid:hclmfUmivnQg9W2z8Lpp4f6Ghac false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:O35152 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998096|imex:IM-23550-23 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 2a (lane 2), 2c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:1F0F3EF230F2538C|rigid:hclmfUmivnQg9W2z8Lpp4f6Ghac false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q9JI51 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7573650|intact:MINT-5225883|uniprotkb:Q9JI52 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:vti1a_rat(display_long)|uniprotkb:Vti1a(gene name)|psi-mi:Vti1a(display_short)|uniprotkb:Vti1l2(gene name synonym)|uniprotkb:Vesicle transport v-SNARE protein Vti1-like 2(gene name synonym)|uniprotkb:Vti1-rp2(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 mint:Q9JI51|interpro:IPR027027|interpro:IPR007705(Vesicle transport v-SNARE)|interpro:IPR010989(t-SNARE)|refseq:NP_075589.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:621490|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005776"(autophagosome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0006914"(autophagy)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016189"(synaptic vesicle to endosome fusion)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0030665"(clathrin-coated vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0044306"(neuron projection terminus)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050882"(voluntary musculoskeletal movement)|go:"GO:0090161"(Golgi ribbon formation)|rcsb pdb:2NPS|reactome:R-RNO-6811438|reactome:R-RNO-6811440|interpro:IPR038407 - - comment:mint|function:V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be required for a fusion step during synaptic vesicle Recycling in the nerve terminal figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:MOylCkFQJsOJ8a/WTTU197k386I10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62839 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q4KM74 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35152 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q5EGY4 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62896 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35165 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998176|imex:IM-23550-26 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:"Fig. 2b (lane 2), 2d"|comment:Vti1a bands are very faint.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:8F90F1475ADF1971|rigid:fQtQKKHX/7FcDJEEIX74EAwgduU false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q63714 uniprotkb:Q62839 intact:EBI-7588697|intact:MINT-4599147 intact:EBI-618335|uniprotkb:F1LSS0 psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:goga2_rat(display_long)|uniprotkb:Golga2(gene name)|psi-mi:Golga2(display_short)|uniprotkb:130 kDa cis-Golgi matrix protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10023062|imex:IM-23550-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) interpro:IPR043937|interpro:IPR043976|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000922"(spindle pole)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005874"(microtubule)|go:"GO:0006486"(protein glycosylation)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0007020"(microtubule nucleation)|go:"GO:0007030"(Golgi organization)|go:"GO:0008017"(microtubule binding)|go:"GO:0008356"(asymmetric cell division)|go:"GO:0010507"(negative regulation of autophagy)|go:"GO:0015031"(protein transport)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030134"(COPII-coated ER to Golgi transport vesicle)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0032580"(Golgi cisterna membrane)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051225"(spindle assembly)|go:"GO:0051289"(protein homotetramerization)|go:"GO:0051645"(Golgi localization)|go:"GO:0060050"(positive regulation of protein glycosylation)|go:"GO:0061676"(importin-alpha family protein binding)|go:"GO:0072686"(mitotic spindle)|go:"GO:0090161"(Golgi ribbon formation)|go:"GO:0090166"(Golgi disassembly)|go:"GO:0090306"(meiotic spindle assembly)|go:"GO:0090307"(mitotic spindle assembly)|reactome:R-RNO-162658|reactome:R-RNO-204005|go:"GO:0007098"(centrosome cycle)|reactome:R-RNO-6807878|reactome:R-RNO-9013148|reactome:R-RNO-9013405|rgd:620282|refseq:NP_072118.2|refseq:XP_006234060.1|interpro:IPR024858|mint:Q62839 - comment:mint - figure legend:Fig. 5a|antagonist:"GM130 NT domain (GOLGA2, Q62839)"|agonist:"p115 (Uso1, P41542)"|comment:"Addition of GM130 NT domain (GOLGA2, Q62839) produced only very faint bands for GM130 full length and p115."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 rogid:zW0U11na/96FACXJccRSnFPMte010116 intact-crc:200AE49572239DB1|rigid:8Agg0jyZU9keOV/enJuhYX8cR3c false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q63714 uniprotkb:P41542 intact:EBI-7588697|intact:MINT-4599147 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:q63714_rat(display_long)|uniprotkb:Golgb1(gene name)|psi-mi:Golgb1(display_short) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10023062|imex:IM-23550-13 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_620240.1|interpro:IPR026202|rgd:708429|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0016021"(integral component of membrane)|go:"GO:0035988"(chondrocyte proliferation)|go:"GO:1905793"(protein localization to pericentriolar material) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - comment:mint - figure legend:Fig. 5a|antagonist:"GM130 NT domain (GOLGA2, Q62839)"|agonist:"p115 (Uso1, P41542)"|comment:"Addition of GM130 NT domain (GOLGA2, Q62839) produced only very faint bands for GM130 full length and p115."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2015/11/10 rogid:PTtaLvlEvCWpq6VB9JPmivyE6Mo10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:200AE49572239DB1|rigid:8Agg0jyZU9keOV/enJuhYX8cR3c false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q4KM74 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021741|imex:IM-23550-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 3c (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:4BDAFAC4E254056F|rigid:yTRXm/1x+HUCgzwgu/IYx23MSG0 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q62896 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021741|imex:IM-23550-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 3c (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:4BDAFAC4E254056F|rigid:yTRXm/1x+HUCgzwgu/IYx23MSG0 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q08851 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021741|imex:IM-23550-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:"Fig. 3c (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:4BDAFAC4E254056F|rigid:yTRXm/1x+HUCgzwgu/IYx23MSG0 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:P41542 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021741|imex:IM-23550-12 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 3c (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:4BDAFAC4E254056F|rigid:yTRXm/1x+HUCgzwgu/IYx23MSG0 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q62931 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021012|imex:IM-23550-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 3a (lanes 3, 7-9, 12, 14), 3b, 8e, 8f"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|agonist:"p115 (Uso1, P41542)"|comment:"Combined addition of p115 and its CC1 domain peptide (3a, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroys them completely (3b). Addition of p115 CC2 domain peptide (3a lane 9 & 3b lane 7) had no effect on the interaction."|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:64972A1F0001C535|rigid:hFPTe0i3h/REQfhXOq2gChNsfs4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q5EGY4 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021012|imex:IM-23550-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 3a (lanes 3, 7-9, 12, 14), 3b, 8e, 8f"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|agonist:"p115 (Uso1, P41542)"|comment:"Combined addition of p115 and its CC1 domain peptide (3a, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroys them completely (3b). Addition of p115 CC2 domain peptide (3a lane 9 & 3b lane 7) had no effect on the interaction."|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:64972A1F0001C535|rigid:hFPTe0i3h/REQfhXOq2gChNsfs4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q08851 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10021012|imex:IM-23550-11 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:"Fig. 3a (lanes 3, 7-9, 12, 14), 3b, 8e, 8f"|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|agonist:"p115 (Uso1, P41542)"|comment:"Combined addition of p115 and its CC1 domain peptide (3a, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroys them completely (3b). Addition of p115 CC2 domain peptide (3a lane 9 & 3b lane 7) had no effect on the interaction."|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:64972A1F0001C535|rigid:hFPTe0i3h/REQfhXOq2gChNsfs4 false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q4KM74 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000925|imex:IM-23550-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 3c (lanes 3, 7-9, 12, 14), 3d"|comment:"Combined addition of p115 and its CC1 domain peptide (3c, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroyed them completely (3d). Addition of p115 CC2 domain peptide (3c, lane 9) had no effect on the interaction."|agonist:"p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:8AD3B46375EE1864|rigid:kFCqH4GOAqk5CHjLdJ7YnYuMVdg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q62896 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000925|imex:IM-23550-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 3c (lanes 3, 7-9, 12, 14), 3d"|comment:"Combined addition of p115 and its CC1 domain peptide (3c, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroyed them completely (3d). Addition of p115 CC2 domain peptide (3c, lane 9) had no effect on the interaction."|agonist:"p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:8AD3B46375EE1864|rigid:kFCqH4GOAqk5CHjLdJ7YnYuMVdg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35165 uniprotkb:Q08851 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000925|imex:IM-23550-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:"Fig. 3c (lanes 3, 7-9, 12, 14), 3d"|comment:"Combined addition of p115 and its CC1 domain peptide (3c, lane 8) and a wash with buffer containing the CC1 domain peptide (3a, lane 12) or 1M KCL (3a, lane 14) abolished the binding of p115 to the complex. Boiling of the re-constituted complexes destroyed them completely (3d). Addition of p115 CC2 domain peptide (3c, lane 9) had no effect on the interaction."|agonist:"p115 (Uso1, P41542), either in reconstitution butter or in wash buffer."|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|antagonist:1M KCl wash|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:8AD3B46375EE1864|rigid:kFCqH4GOAqk5CHjLdJ7YnYuMVdg false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q62931 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000796|imex:IM-23550-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 3a (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:8D0F9D9B28DECA1A|rigid:IskdvDuJnxMOSwiK3oWPdzju1Zw false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q5EGY4 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000796|imex:IM-23550-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 3a (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:8D0F9D9B28DECA1A|rigid:IskdvDuJnxMOSwiK3oWPdzju1Zw false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:Q08851 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000796|imex:IM-23550-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 - - - figure legend:"Fig. 3a (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:8D0F9D9B28DECA1A|rigid:IskdvDuJnxMOSwiK3oWPdzju1Zw false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:O35152 uniprotkb:P41542 intact:EBI-9997695|uniprotkb:Q6PCU6 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10000796|imex:IM-23550-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 3a (lanes 4-6, 10-11, 13)"|comment:"Addition of p115 CC2 domain peptide (lanes 10 & 13) had no effect on the interaction."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:8D0F9D9B28DECA1A|rigid:IskdvDuJnxMOSwiK3oWPdzju1Zw false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 - - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35165 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997716|uniprotkb:O08566|uniprotkb:Q6GSW2|ensembl:ENSRNOP00000005046 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr2_rat(display_long)|uniprotkb:Gosr2(gene name)|psi-mi:Gosr2(display_short)|uniprotkb:Gs27(gene name synonym)|uniprotkb:27 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:Membrin(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_113873.1|rgd:62079|ensembl:ENSRNOG00000003506(gene)|ensembl:ENSRNOT00000005046(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006623"(protein targeting to vacuole)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0006896"(Golgi to vacuole transport)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0031201"(SNARE complex)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR027027|interpro:IPR038407|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438 - - - figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:Fspz2ZRLScay81Bfj6nicSM9QCA10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q4KM74 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-920764|ensembl:ENSRNOP00000025327 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:sc22b_rat(display_long)|uniprotkb:Sec22b(gene name)|psi-mi:Sec22b(display_short)|uniprotkb:SEC22 vesicle-trafficking protein homolog B(gene name synonym)|uniprotkb:SEC22 vesicle-trafficking protein-like 1(gene name synonym)|uniprotkb:ER-Golgi SNARE of 24 kDa(gene name synonym)|uniprotkb:Sec22l1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:1309988|refseq:NP_001020857.1|ensembl:ENSRNOG00000018673(gene)|ensembl:ENSRNOT00000025327(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005793"(endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006890"(retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030137"(COPI-coated vesicle)|go:"GO:0031201"(SNARE complex)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0042470"(melanosome)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:1902902"(negative regulation of autophagosome assembly)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|interpro:IPR044565|reactome:R-RNO-1236974|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6811434 - - - figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:DRKtD169T9eYZ2kFufLapidvcKs10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:O35152 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997695|uniprotkb:Q6PCU6 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1l_rat(display_long)|uniprotkb:Bet1l(gene name)|psi-mi:Bet1l(display_short)|uniprotkb:Gs15(gene name synonym)|uniprotkb:Golgi SNARE with a size of 15 kDa(gene name synonym)|uniprotkb:Vesicle transport protein GOS15(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062241.2|refseq:XP_006230636.1|interpro:IPR000727(Target SNARE coiled-coil region)|rgd:71051|reactome:R-RNO-6807878|reactome:R-RNO-6811438|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005768"(endosome)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005795"(Golgi stack)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0015031"(protein transport)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:2000156"(regulation of retrograde vesicle-mediated transport, Golgi to ER)|go:"GO:0005798"(Golgi-associated vesicle)|interpro:IPR039897|interpro:IPR039899 - - - figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:TkC+yWZcu8CCQEnS3Cq1c0O416k10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62896 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-9997738|ensembl:ENSRNOP00000014810 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:bet1_rat(display_long)|uniprotkb:Bet1(gene name)|psi-mi:Bet1(display_short)|uniprotkb:Golgi vesicular membrane-trafficking protein p18(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 refseq:NP_062124.1|rgd:2203|ensembl:ENSRNOG00000011008(gene)|ensembl:ENSRNOT00000014810(transcript)|go:"GO:0000137"(Golgi cis cisterna)|go:"GO:0000138"(Golgi trans cisterna)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030173"(integral component of Golgi membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031985"(Golgi cisterna)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR039897|interpro:IPR039899|reactome:R-RNO-204005|reactome:R-RNO-6807878 - - - figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:ciS6OKcBDKAbbVriifH8li7hiGo10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q5EGY4 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-915507|uniprotkb:O35487|ensembl:ENSRNOP00000020693 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:ykt6_rat(display_long)|uniprotkb:Ykt6(gene name)|psi-mi:Ykt6(display_short) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-9998270|imex:IM-23550-28 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:70897|refseq:NP_113880.2|ensembl:ENSRNOG00000014785(gene)|ensembl:ENSRNOT00000020693(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006903"(vesicle targeting)|go:"GO:0006904"(vesicle docking involved in exocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030659"(cytoplasmic vesicle membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0043025"(neuronal cell body)|go:"GO:0097440"(apical dendrite)|go:"GO:0097441"(basal dendrite)|interpro:IPR001388(Synaptobrevin)|interpro:IPR010908(Longin)|interpro:IPR011012(Longin-like)|interpro:IPR042855|rcsb pdb:3KYQ|reactome:R-RNO-204005|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|go:"GO:0042147"(retrograde transport, endosome to Golgi)|reactome:R-RNO-9013148|reactome:R-RNO-9013149|reactome:R-RNO-9013408|reactome:R-RNO-9013423 - - - figure legend:"Fig. 2f (lane 2)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/09 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:zvwtiPzYkzRfETeKT/2k+q7Uvwk10116 intact-crc:4E7C32CD94446554|rigid:fS3pRvbdZcBOLYvo5CP+B1DCSOI false - - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024964|imex:IM-23550-17 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 7a (top panel, lane 4), 7b (top panel lanes 4-8), 7c, 8a (top panel lanes 4, 9-11, middle panel lanes 4, 9-11), 8b (middle panel), 8c (lanes 4 & 5), 8d"|comment:"Binding of GOS28 and syntaxin-5 is weak without the addition of p115 (7a lane 4, 7b lanes 4-8, 8a all panels, 8d). Addition of p115 CC1, CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 5-8, 8a bottom panel). Addition of p115 CC1 domain peptides (either in binding or wash buffer) with full length p115 disrupted the binding of p115 to the complex (7b, lane 10, 8a middle panel lanes 10-11, 8b middle panel). Aggitation also disrupted the binding of p115 to the complex (8c lane 5)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:402A4ECE3D0DBDE5|rigid:yC0NkdZHzApPyc3H+2fJXnQPIXU false his tag:?-?|sufficient binding region:2..2-229..229 sufficient binding region:1..1-333..333|glutathione s tranferase tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10043662|imex:IM-23550-29 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:Fig. 7e|comment:"Addition of increasing amounts of p115 head, tail (T, 637-930 aa) and tail plus acidic region (TA, 637-959 aa) increase the interaction of GOS28-syntaxin-5 but >1500 nM of T or TA constructs reduce the binding efficacy. Bait/prey relationship is unknown."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:3F87DA69E23F604C|rigid:yC0NkdZHzApPyc3H+2fJXnQPIXU false sufficient binding region:2..2-229..229|his tag:?-? sufficient binding region:1..1-333..333|glutathione s tranferase tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024926|imex:IM-23550-16 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 7a (bottom panel, lane 4), 7b (bottom panel, lanes 4-8)."|comment:"Addition of p115 CC1, CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 5-8)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:277E6E25AB8163F6|rigid:yC0NkdZHzApPyc3H+2fJXnQPIXU false his tag:?-?|sufficient binding region:2..2-229..229 glutathione s tranferase tag:?-?|sufficient binding region:1..1-333..333 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:P41542 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10002132|imex:IM-23550-6 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 go:"GO:0000139"(Golgi membrane) - - figure legend:"Fig. 6b (bottom left), 6c"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) kd:1.8x10^-9(molar) 2014/12/05 2014/12/16 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:3715ED663B326B7E|rigid:A4gLIClNDtwkiCMTf7UiZRp2qZY false sufficient binding region:1..1-333..333|glutathione s tranferase tag:?-? sufficient binding region:637-930 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:Q62931 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024704|imex:IM-23550-14 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 go:"GO:0000139"(Golgi membrane) - comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport figure legend:"Fig. 7a (top panel, lanes 5-10), 7b (top panel lanes 9, 11-13), 7d, 8a (top panel lanes 5 & 6, middle panel lanes 5 & 6, bottom panel lanes 5 & 6), 8b (left & right panels)"|comment:"Increasing amounts of p115 increase the binding of GOS28 (7a, 8a). Addition of p115 CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 11-13, 8a bottom panel, 8b right panel)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 intact-crc:3C6186148F8128FD|rigid:5k9bjVp32Z+Dcmum1uS/820Gk4k false glutathione s tranferase tag:?-?|sufficient binding region:1..1-333..333 his tag:?-?|sufficient binding region:2..2-229..229 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q08851 uniprotkb:P41542 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024704|imex:IM-23550-14 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 go:"GO:0000139"(Golgi membrane) - - figure legend:"Fig. 7a (top panel, lanes 5-10), 7b (top panel lanes 9, 11-13), 7d, 8a (top panel lanes 5 & 6, middle panel lanes 5 & 6, bottom panel lanes 5 & 6), 8b (left & right panels)"|comment:"Increasing amounts of p115 increase the binding of GOS28 (7a, 8a). Addition of p115 CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 11-13, 8a bottom panel, 8b right panel)."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:3C6186148F8128FD|rigid:5k9bjVp32Z+Dcmum1uS/820Gk4k false glutathione s tranferase tag:?-?|sufficient binding region:1..1-333..333 - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:P41542 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10001587|imex:IM-23550-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 6a (bottom), 6d"|comment:Addition of CC1 peptide reduced the p115 band but not the syntaxin-5 band. Addition of CC2 or CC3 domain peptides made no difference to the interaction.|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) kd:1.5x10^-9(molar) 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:7B6B475CE2D5F703|rigid:wUxnzVmvnjGeaujuZmmR2mEGii8 false sufficient binding region:2..2-229..229|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P41542 uniprotkb:Q08851 intact:EBI-4423297|ensembl:ENSRNOP00000003277 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10001059|imex:IM-23550-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 go:"GO:0000139"(Golgi membrane) - - figure legend:"Fig. 6a (top)"|comment:Addition of CC1 peptide reduced the p115 band but not the syntaxin-5 band. Addition of CC2 or CC3 domain peptides made no difference to the interaction.|antagonist:"CC1 domain (637-699) of p115 (Uso1, P41542)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:50BE50D18E84E672|rigid:A4gLIClNDtwkiCMTf7UiZRp2qZY false - sufficient binding region:1..1-333..333|his tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:Q08851 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-2028244|uniprotkb:A0JPL4|uniprotkb:O55200|ensembl:ENSRNOP00000025664 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:stx5_rat(display_long)|uniprotkb:Stx5(gene name)|psi-mi:Stx5(display_short)|uniprotkb:Stx5a(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024852|imex:IM-23550-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:68426|refseq:XP_006231059.1|refseq:NP_113892.2|ensembl:ENSRNOG00000018847(gene)|ensembl:ENSRNOT00000025664(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0000149"(SNARE binding)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0012505"(endomembrane system)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0031982"(vesicle)|go:"GO:0033116"(endoplasmic reticulum-Golgi intermediate compartment membrane)|go:"GO:0034498"(early endosome to Golgi transport)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045732"(positive regulation of protein catabolic process)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048278"(vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0090166"(Golgi disassembly)|go:"GO:1903358"(regulation of Golgi organization)|interpro:IPR000727(Target SNARE coiled-coil region)|interpro:IPR006012(Syntaxin/epimorphin, conserved site)|interpro:IPR010989(t-SNARE)|interpro:IPR021538|reactome:R-RNO-204005|reactome:R-RNO-5694530|reactome:R-RNO-6807878|reactome:R-RNO-6811438|reactome:R-RNO-8980692|reactome:R-RNO-9013106|reactome:R-RNO-9013408|reactome:R-RNO-9609523 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 7a (bottom panel, lanes 5-10), 7b (bottom, panel lanes 9-13)"|comment:"p115 (Uso1) is not required for efficient binding of GOS28 and syntaxin-5 (7a). Addition of p115 CC1, CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 11-13). Addition of p115 CC1 domain reduced the binding of p115 to the complex."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:ulHbbXxIwp4tCaKzXM2/+emqpMQ10116 intact-crc:223B9C33FB65F139|rigid:5k9bjVp32Z+Dcmum1uS/820Gk4k false sufficient binding region:2..2-229..229|his tag:?-? sufficient binding region:1..1-333..333|glutathione s tranferase tag:?-? - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:P41542 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10024852|imex:IM-23550-15 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 7a (bottom panel, lanes 5-10), 7b (bottom, panel lanes 9-13)"|comment:"p115 (Uso1) is not required for efficient binding of GOS28 and syntaxin-5 (7a). Addition of p115 CC1, CC2 or CT domains, or GM130 NT domain had no effect on the interaction (7b, lanes 11-13). Addition of p115 CC1 domain reduced the binding of p115 to the complex."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10116(rat-liver)|taxid:10116(rattus norvegicus liver) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:223B9C33FB65F139|rigid:5k9bjVp32Z+Dcmum1uS/820Gk4k false sufficient binding region:2..2-229..229|his tag:?-? - - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:Q62931 uniprotkb:P41542 intact:EBI-7837133|ensembl:ENSRNOP00000068434|intact:MINT-4569995|uniprotkb:A0JN01 intact:EBI-4423297|ensembl:ENSRNOP00000003277 psi-mi:gosr1_rat(display_long)|uniprotkb:Gosr1(gene name)|psi-mi:Gosr1(display_short)|uniprotkb:Gs28(gene name synonym)|uniprotkb:28 kDa Golgi SNARE protein(gene name synonym)|uniprotkb:28 kDa cis-Golgi SNARE p28(gene name synonym) psi-mi:uso1_rat(display_long)|uniprotkb:Uso1(gene name)|psi-mi:Uso1(display_short)|uniprotkb:Vdp(gene name synonym)|uniprotkb:Protein USO1 homolog(gene name synonym)|uniprotkb:Transcytosis-associated protein(gene name synonym)|uniprotkb:Vesicle-docking protein(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Shorter et al. (2002) pubmed:11927603|imex:IM-23550 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10001755|imex:IM-23550-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSRNOG00000003971(gene)|ensembl:ENSRNOT00000077063(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005484"(SNAP receptor activity)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005797"(Golgi medial cisterna)|go:"GO:0005801"(cis-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006906"(vesicle fusion)|go:"GO:0015031"(protein transport)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031201"(SNARE complex)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0048209"(regulation of vesicle targeting, to, from or within Golgi)|interpro:IPR023601|mint:Q62931|reactome:R-RNO-6807878|reactome:R-RNO-6811438|rgd:71093|refseq:NP_446036.1 rgd:621604|refseq:NP_062252.1|ensembl:ENSRNOG00000002301(gene)|ensembl:ENSRNOT00000003277(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001650"(fibrillar center)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005795"(Golgi stack)|go:"GO:0005829"(cytosol)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006888"(endoplasmic reticulum to Golgi vesicle-mediated transport)|go:"GO:0006891"(intra-Golgi vesicle-mediated transport)|go:"GO:0007030"(Golgi organization)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0012507"(ER to Golgi transport vesicle membrane)|go:"GO:0032252"(secretory granule localization)|go:"GO:0045056"(transcytosis)|go:"GO:0048211"(Golgi vesicle docking)|go:"GO:0048280"(vesicle fusion with Golgi apparatus)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0061025"(membrane fusion)|go:"GO:1900076"(regulation of cellular response to insulin stimulus)|interpro:IPR000225(Armadillo)|interpro:IPR006953(Uso1/p115 like vesicle tethering protein, head region)|interpro:IPR006955(Uso1/p115 like vesicle tethering protein, C-terminal)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR041209|reactome:R-RNO-204005|reactome:R-RNO-6807878 go:"GO:0000139"(Golgi membrane) comment:mint|function:Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport - figure legend:"Fig. 6b (top left)"|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2014/12/05 2014/12/16 rogid:A1I17p7sB3hZGW3y7AjPQI0TPSg10116 rogid:lw2vLmxkQMppysDgr/JQoDwzAFE10116 intact-crc:1EED52CCB1D80975|rigid:wUxnzVmvnjGeaujuZmmR2mEGii8 false his tag:?-?|sufficient binding region:2..2-229..229 sufficient binding region:637-930 - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)