#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q7A2B0	uniprotkb:Q7A2B0	intact:EBI-9005887|ensemblbacteria:BAB75409	intact:EBI-9005887|ensemblbacteria:BAB75409	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:"MI:0071"(molecular sieving)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9005882|imex:IM-22018-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f2a sf3a|comment:"In SEC, DevB appears to be in an oligomeric equilibrium between monomers, dimers, trimers and  hexamers. The majority of reference DevB (Fig. 1D and Tab. 1) was eluted as hexamer or monomer   (Fig. 2A; upper solid line). In contrast, hexamer formation of DevB lacking the entire α-helical  domain (B-αHD in Fig. 1D and Tab. 1) was hardly observable (Fig. 2A; lower solid line). This DevB  variant could not efficiently interact with immobilized reference DevB in SPR (Fig. 2B; B-αHD).  Removing the distal part of DevB´s α-helical domain (B-DαHD in Fig. 1D and Tab. 1) showed no  remarkable effect on hexamer formation as observed in SEC (Fig. 2A; dashed line), and B-DαHD  retained ~80% of interaction with immobilized reference DevB, as compared to reference DevB  itself as prey (Fig. 2B; B and B-DαHD). However, deleting the proximal part of DevB´s α-helical  domain (B-PαHD; Fig. 1D and Tab. 1) decreased this interaction to ~25% (Fig. 2B; B-PαHD). This  variant did not form substantial amounts of hexamers (Fig. 2A; dotted line).  DevB lacking the β-barrel domain (B-βBD; Fig. 1D and Tab. 1) formed ~75% less hexamers than  reference DevB (Fig. 2A; dot-dot-dashed line). Replacing central parts of the lipoyl domain (BxLipD  in Fig. 1D and Tab. 1) led to hardly detectable DevB hexamers (Fig. 2A; dot-dashed line).  Consistent with these data, both constructs had decreased affinities to immobilized reference DevB   (Fig. 2B; B-βBD at ~45% and BxLipD at ~30%).  In summary, all domains of DevB, except the distal part of the α-helical domain, are required for  stabilization of the inter-MFP interactions, and therefore facilitate hexamer formation."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	intact-crc:ABA2D7F6AAFFF404|rigid:Ss4Mi2sX0QOA7PkcDfymf0rdnnA	false	binding-associated region:115-216,249-345	binding-associated region:115-216,249-345	6	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q7A2B0	uniprotkb:Q7A2B0	intact:EBI-9005887|ensemblbacteria:BAB75409	intact:EBI-9005887|ensemblbacteria:BAB75409	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9006509|imex:IM-22018-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f2b|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	intact-crc:AF43F533C742A178|rigid:9pqCJYU6mjNUVQ0r9uAbx35GG+Y	false	binding-associated region:115-216,249-345	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8YT39	uniprotkb:Q7A2B0	intact:EBI-9006622|ensemblbacteria:BAB74586	intact:EBI-9005887|ensemblbacteria:BAB75409	psi-mi:q8yt39_noss1(display_long)|uniprotkb:alr2887(locus name)	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9006639|imex:IM-22018-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003423(Outer membrane efflux protein)|refseq:WP_010997038.1|ensemblbacteria:BAB74586(gene)|ensemblbacteria:BAB74586(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f3b sf5b sf2|comment:"We also investigated structural criteria of TolC required for binding to DevB. We quantified the  interaction of TolC variants lacking the native α-helical tips (replaced by GGS/SSG; Fig. 1C) with   immobilized reference DevB via SPR analysis. In line with the effect of tip-mutations of DevB (Fig.   3A, constructs with *), tip-mutated TolC could not efficiently interact with surface-bound reference  DevB in SPR experiments (Fig. 3B; D*145 )."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:SFrDU59oN6VjJkisblU/qQi1Vzo103690	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	intact-crc:487809359C3D565F|rigid:HiRBPSfGHzDCz/v1SazvSBpt4xg	false	mutation disrupting interaction:688-695|mutation disrupting interaction strength:478-485	his tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8YT39	uniprotkb:Q7A2B0	intact:EBI-9006622|ensemblbacteria:BAB74586	intact:EBI-9005887|ensemblbacteria:BAB75409	psi-mi:q8yt39_noss1(display_long)|uniprotkb:alr2887(locus name)	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9006620|imex:IM-22018-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR003423(Outer membrane efflux protein)|refseq:WP_010997038.1|ensemblbacteria:BAB74586(gene)|ensemblbacteria:BAB74586(transcript)|go:"GO:0015562"(efflux transmembrane transporter activity)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f3a sf5a|comment:"all DevB’s lacking the native α-helical tips (B*, B-DαHD*,  137 B-PαHD*, BxLipD*, and B-βBD*; see Fig. 1D and Tab. 1) could hardly interact with immobilized  138 reference TolC in SPR experiments (Fig. 3A). The affinities of native α-helical tip variants of DevB  139 toward TolC (B-DαHD > B-βBD > B-PαHD, BxLipD > B-αHD; Fig. 3A) correlated well with the  140 ability to hexamerize (B-DαHD > B-βBD > B-PαHD, BxLipD > B-αHD; Fig. 2)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:SFrDU59oN6VjJkisblU/qQi1Vzo103690	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	intact-crc:73DEA732E5E2992F|rigid:HiRBPSfGHzDCz/v1SazvSBpt4xg	false	his tag:?-?	binding-associated region:115-216,249-345	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q7A2A8	uniprotkb:Q7A2A9	intact:EBI-9006693|ensemblbacteria:BAB75411	intact:EBI-9006685|ensemblbacteria:BAB75410	psi-mi:q7a2a8_noss1(display_long)|uniprotkb:devA(gene name)|psi-mi:devA(display_short)	psi-mi:q7a2a9_noss1(display_long)|uniprotkb:devC(gene name)|psi-mi:devC(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9006666|imex:IM-22018-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR017911|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR014324(ABC transporter ATP-binding subunit, DevA type)|interpro:IPR003439(ABC transporter-like)|interpro:IPR017871|interpro:IPR027417|refseq:WP_010997855.1|ensemblbacteria:BAB75411(gene)|ensemblbacteria:BAB75411(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0042626"(ATPase-coupled transmembrane transporter activity)	interpro:IPR005891(DevC protein)|interpro:IPR003838(Protein of unknown function DUF214, permase predicted)|refseq:WP_010997854.1|ensemblbacteria:BAB75410(gene)|ensemblbacteria:BAB75410(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)	-	-	-	figure legend:f4a sf6|comment:"The DevB variants lacking the proximal or the entire α-helical domain (B-αHD and B-PαHD; Fig.  1D and Tab. 1) were impaired in interacting with immobilized DevAC by ~70% (Fig. 4A). Mutating  168 the lipoyl domain or the β-barrel domain (BxLipD and B-βBD; Fig. 1D and Tab. 1) resulted in a  decrease of 90% as compared to reference DevB (Fig. 4A). The SPR-data imply (i) a contact  interface of DevB to DevAC involving both the lipoyl and the β-barrel domain, and it reflects (ii) a  171 stabilizing effect of DevB hexamers on the binding to DevAC (B-DαHD > B-PαHD, B-αHD > B-   βBD, BxLipD; Fig. 4A). This behavior is similar to the binding of DevB to TolC (B-DαHD > B-βBD  > B-PαHD, BxLipD > B-αHD; Fig. 2)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:Csd9zLNPw/jaNfCGW0nn355cfb0103690	rogid:1G74wSpozaOWjtFKCOvGr6uG0zg103690	intact-crc:251C6E92D7695ADF|rigid:zbMdNdQEEn2brf0jqO6rvRvmf0I	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q7A2A8	uniprotkb:Q7A2B0	intact:EBI-9006693|ensemblbacteria:BAB75411	intact:EBI-9005887|ensemblbacteria:BAB75409	psi-mi:q7a2a8_noss1(display_long)|uniprotkb:devA(gene name)|psi-mi:devA(display_short)	psi-mi:q7a2b0_noss1(display_long)|uniprotkb:devB(gene name)|psi-mi:devB(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Staron et al. (2013)	imex:IM-22018|pubmed:24361095	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	taxid:103690(noss1)|taxid:103690("Nostoc sp. (strain PCC 7120 / UTEX 2576)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9006666|imex:IM-22018-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR017911|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR014324(ABC transporter ATP-binding subunit, DevA type)|interpro:IPR003439(ABC transporter-like)|interpro:IPR017871|interpro:IPR027417|refseq:WP_010997855.1|ensemblbacteria:BAB75411(gene)|ensemblbacteria:BAB75411(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0042626"(ATPase-coupled transmembrane transporter activity)	interpro:IPR014315(ABC transporter membrane fusion protein, DevB type)|refseq:WP_010997853.1|interpro:IPR039562|ensemblbacteria:BAB75409(gene)|ensemblbacteria:BAB75409(transcript)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f4a sf6|comment:"The DevB variants lacking the proximal or the entire α-helical domain (B-αHD and B-PαHD; Fig.  1D and Tab. 1) were impaired in interacting with immobilized DevAC by ~70% (Fig. 4A). Mutating  168 the lipoyl domain or the β-barrel domain (BxLipD and B-βBD; Fig. 1D and Tab. 1) resulted in a  decrease of 90% as compared to reference DevB (Fig. 4A). The SPR-data imply (i) a contact  interface of DevB to DevAC involving both the lipoyl and the β-barrel domain, and it reflects (ii) a  171 stabilizing effect of DevB hexamers on the binding to DevAC (B-DαHD > B-PαHD, B-αHD > B-   βBD, BxLipD; Fig. 4A). This behavior is similar to the binding of DevB to TolC (B-DαHD > B-βBD  > B-PαHD, BxLipD > B-αHD; Fig. 2)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/12/03	2014/10/16	rogid:Csd9zLNPw/jaNfCGW0nn355cfb0103690	rogid:Ywr99ER5gPM3EfqyEm3GSGvD2cE103690	intact-crc:251C6E92D7695ADF|rigid:zbMdNdQEEn2brf0jqO6rvRvmf0I	false	-	binding-associated region:115-216,249-345	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)