#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q8BGG7	uniprotkb:P43403	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:"MI:0096"(pull down)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846412|imex:IM-21762-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	-	-	-	figure legend:f1c|comment:"We began by assessing the interaction between a Zap-70-derived  phosphopeptide (GSVYESPpYSDPEEL) and the different Sts-1PGM mutants described above. Pulldown  assays were performed in which phosphopeptide-coupled beads were added to lysates prepared  from cells expressing wild-type or mutant Sts-1PGM, and bound proteins were assessed by immunoblot  analysis. We observed that four Sts-1PGM mutants interacted stably with the bound phosphopeptide, asevidenced by their ability to be isolated from the lysate (Figure 1C). Interestingly, the four mutants  appeared to interact with the phospho-peptide beads with substantially different affinities. Specifically,  Sts-1PGM H380C single mutant exhibited very weak interaction with the phospho-peptide beads, while  the double mutants Sts-1PGM H380C/E490A and Sts-1PGM H380C/E490Q appeared to have the strongest  affinity and the Sts-1PGM H380C/R462A/E490Q triple mutant was precipitated to an intermediate degree.  Full-length Sts-1 variants also displayed similar binding characteristics (data not shown), with double  mutants H380C/E490A and H380C/E490Q (henceforth referred to as CA and CQ respectively)  displaying increased binding capabilities than the single mutant H380C (henceforth referred to as C).  These results suggest that the CA and CQ mutants could serve as significantly better substrate-trapping  enzymes than the C mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/08	2014/10/16	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	intact-crc:26CB20C35712DC86|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	flag tag:?-?|binding-associated region:369-638|trapping mutant:380-380	binding-associated region:312-325|porphyrin modified residue:319-319	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8BGG7	uniprotkb:P43403	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:"MI:0096"(pull down)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846469|imex:IM-21762-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	-	-	-	figure legend:f1c|comment:"We began by assessing the interaction between a Zap-70-derived  phosphopeptide (GSVYESPpYSDPEEL) and the different Sts-1PGM mutants described above. Pulldown  assays were performed in which phosphopeptide-coupled beads were added to lysates prepared  from cells expressing wild-type or mutant Sts-1PGM, and bound proteins were assessed by immunoblot  analysis. We observed that four Sts-1PGM mutants interacted stably with the bound phosphopeptide, asevidenced by their ability to be isolated from the lysate (Figure 1C). Interestingly, the four mutants  appeared to interact with the phospho-peptide beads with substantially different affinities. Specifically,  Sts-1PGM H380C single mutant exhibited very weak interaction with the phospho-peptide beads, while  the double mutants Sts-1PGM H380C/E490A and Sts-1PGM H380C/E490Q appeared to have the strongest  affinity and the Sts-1PGM H380C/R462A/E490Q triple mutant was precipitated to an intermediate degree.  Full-length Sts-1 variants also displayed similar binding characteristics (data not shown), with double  mutants H380C/E490A and H380C/E490Q (henceforth referred to as CA and CQ respectively)  displaying increased binding capabilities than the single mutant H380C (henceforth referred to as C).  These results suggest that the CA and CQ mutants could serve as significantly better substrate-trapping  enzymes than the C mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/08	2014/10/16	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	intact-crc:BB388635B9304509|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	trapping mutant:490-490,380-380|binding-associated region:369-638|flag tag:?-?	binding-associated region:312-325|porphyrin modified residue:319-319	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P43403	uniprotkb:Q8BGG7	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:"MI:0096"(pull down)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846485|imex:IM-21762-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	-	-	-	figure legend:f1c|comment:"We began by assessing the interaction between a Zap-70-derived  phosphopeptide (GSVYESPpYSDPEEL) and the different Sts-1PGM mutants described above. Pulldown  assays were performed in which phosphopeptide-coupled beads were added to lysates prepared  from cells expressing wild-type or mutant Sts-1PGM, and bound proteins were assessed by immunoblot  analysis. We observed that four Sts-1PGM mutants interacted stably with the bound phosphopeptide, asevidenced by their ability to be isolated from the lysate (Figure 1C). Interestingly, the four mutants  appeared to interact with the phospho-peptide beads with substantially different affinities. Specifically,  Sts-1PGM H380C single mutant exhibited very weak interaction with the phospho-peptide beads, while  the double mutants Sts-1PGM H380C/E490A and Sts-1PGM H380C/E490Q appeared to have the strongest  affinity and the Sts-1PGM H380C/R462A/E490Q triple mutant was precipitated to an intermediate degree.  Full-length Sts-1 variants also displayed similar binding characteristics (data not shown), with double  mutants H380C/E490A and H380C/E490Q (henceforth referred to as CA and CQ respectively)  displaying increased binding capabilities than the single mutant H380C (henceforth referred to as C).  These results suggest that the CA and CQ mutants could serve as significantly better substrate-trapping  enzymes than the C mutant."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/08	2014/10/16	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	intact-crc:14071ECDA9B3B506|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	binding-associated region:312-325|porphyrin modified residue:319-319	binding-associated region:369-638|trapping mutant:490-490,380-380|flag tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:P43403	uniprotkb:Q8BGG7	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:"MI:0096"(pull down)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8847821|imex:IM-21762-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	-	-	-	figure legend:f4a f4b f4c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/08	2014/10/16	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	intact-crc:B8D86C46643BEF74|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	phosphorylated residue:319-319	trapping mutant:490-490,380-380|maltose binding protein tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P43403	uniprotkb:Q8BGG7	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:"MI:0096"(pull down)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8847874|imex:IM-21762-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	-	-	-	figure legend:f4b f4c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/08	2014/10/16	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	intact-crc:A905F5A4FFA2CE27|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	phosphorylated residue:319-319	trapping mutant:380-380,490-490|maltose binding protein tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8BGG7	uniprotkb:P43403	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8847889|imex:IM-21762-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	-	-	-	figure legend:f4d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2013/11/08	2014/10/16	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	intact-crc:9A939EE81DC7A884|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	trapping mutant:380-380,490-490|flag tag:?-?	mutation disrupting interaction:319-319|t7 tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:P43403	uniprotkb:Q8BGG7	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846575|imex:IM-21762-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	-	-	-	figure legend:f3c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2013/11/08	2014/10/16	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	intact-crc:9A978AF81DC7A884|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	-	flag tag:?-?|binding-associated region:369-638|trapping mutant:380-380,490-490	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:P43403	uniprotkb:Q8BGG7	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846514|imex:IM-21762-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	-	-	-	figure legend:f3c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2013/11/08	2014/10/16	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	intact-crc:F7431D80775DF978|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	-	flag tag:?-?|binding-associated region:369-638|trapping mutant:380-380,490-490	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8BGG7	uniprotkb:P43403	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846625|imex:IM-21762-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	-	-	-	figure legend:f3c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2013/11/08	2014/10/16	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	intact-crc:76E9B5A925818EDE|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	flag tag:?-?|trapping mutant:380-380,490-490|binding-associated region:369-638	-	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q8BGG7	uniprotkb:P43403	intact:EBI-8846415|uniprotkb:Q3U8Z2|uniprotkb:Q8BMW9|ensembl:ENSMUSP00000043865	intact:EBI-1211276|uniprotkb:A6NFP4|uniprotkb:Q6PIA4|uniprotkb:Q8IXD6|uniprotkb:Q9UBS6|ensembl:ENSP00000264972	psi-mi:ubs3b_mouse(display_long)|uniprotkb:Ubash3b(gene name)|psi-mi:Ubash3b(display_short)|uniprotkb:Sts1(gene name synonym)|uniprotkb:Cbl-interacting protein p70(gene name synonym)|uniprotkb:Suppressor of T-cell receptor signaling 1(gene name synonym)|uniprotkb:T-cell ubiquitin ligand 2(gene name synonym)|uniprotkb:Tyrosine-protein phosphatase STS1/TULA2(gene name synonym)	psi-mi:zap70_human(display_long)|uniprotkb:ZAP70(gene name)|psi-mi:ZAP70(display_short)|uniprotkb:SRK(gene name synonym)|uniprotkb:Syk-related tyrosine kinase(gene name synonym)|uniprotkb:70 kDa zeta-chain associated protein(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Luis et al. (2013)	pubmed:24256567|imex:IM-21762	taxid:10090(mouse)|taxid:10090(Mus musculus)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8846602|imex:IM-21762-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_017169101.1|refseq:NP_789830.1|refseq:XP_006510700.1|ensembl:ENSMUSG00000032020(gene)|ensembl:ENSMUST00000044155(transcript)|go:"GO:0004725"(protein tyrosine phosphatase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006469"(negative regulation of protein kinase activity)|go:"GO:0009968"(negative regulation of signal transduction)|go:"GO:0030168"(platelet activation)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0035335"(peptidyl-tyrosine dephosphorylation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0043393"(regulation of protein binding)|go:"GO:0045670"(regulation of osteoclast differentiation)|go:"GO:0045671"(negative regulation of osteoclast differentiation)|go:"GO:0045779"(negative regulation of bone resorption)|go:"GO:0051219"(phosphoprotein binding)|go:"GO:0051279"(regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070527"(platelet aggregation)|go:"GO:0090331"(negative regulation of platelet aggregation)|interpro:IPR001452(Src homology-3)|interpro:IPR009060(UBA-like)|interpro:IPR013078(Phosphoglycerate mutase)|interpro:IPR015940(Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote)|interpro:IPR029033|interpro:IPR035632|interpro:IPR036028|mint:Q8BGG7|rcsb pdb:2H0Q|rcsb pdb:2IKQ|rcsb pdb:3MBK	refseq:NP_001070.2|refseq:NP_997402.1|dip:DIP-38781N|ensembl:ENSG00000115085(gene)|ensembl:ENST00000264972(transcript)|go:"GO:0001772"(immunological synapse)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006955"(immune response)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030154"(cell differentiation)|go:"GO:0030217"(T cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0043366"(beta selection)|go:"GO:0045059"(positive thymic T cell selection)|go:"GO:0045060"(negative thymic T cell selection)|go:"GO:0045087"(innate immune response)|go:"GO:0045582"(positive regulation of T cell differentiation)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050852"(T cell receptor signaling pathway)|go:"GO:0070489"(T cell aggregation)|go:"GO:0072678"(T cell migration)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P43403|rcsb pdb:1FBV|rcsb pdb:1M61|rcsb pdb:1U59|rcsb pdb:2CBL|rcsb pdb:2OQ1|rcsb pdb:2OZO|rcsb pdb:2Y1N|rcsb pdb:3ZNI|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4K2R|rcsb pdb:4XZ0|rcsb pdb:4XZ1|rcsb pdb:5O76|reactome:R-HSA-202430|reactome:R-HSA-202433|reactome:R-HSA-9013407|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0042110"(T cell activation)	-	-	-	figure legend:f3d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2013/11/08	2014/10/16	rogid:h0Mu6xceMTaoyJgWu+xhMTvNAds10090	rogid:21kVb/nxY2BBZPBNVwWba+qUOgc9606	intact-crc:4E97A006947955E7|rigid:SzFTZwK1PVN33knSCraYj88+ae4	false	flag tag:?-?|binding-associated region:369-638|trapping mutant:380-380,490-490	-	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0113"(western blot)