#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:O60674	uniprotkb:P42224	intact:EBI-518647|uniprotkb:O14636|uniprotkb:O75297|intact:EBI-28977715|ensembl:ENSP00000371067	intact:EBI-1057697|uniprotkb:Q53S88|uniprotkb:Q53XW4|uniprotkb:B2RCA0|uniprotkb:Q9UDL5|uniprotkb:A8K989|uniprotkb:D2KFR8|uniprotkb:Q68D00|uniprotkb:D3DPI7|ensembl:ENSP00000354394|ensembl:ENSP00000386244|ensembl:ENSP00000388240|ensembl:ENSP00000438703	psi-mi:jak2_human(display_long)|uniprotkb:Janus kinase 2(gene name synonym)|uniprotkb:JAK2(gene name)|psi-mi:JAK2(display_short)	psi-mi:stat1_human(display_long)|uniprotkb:STAT1(gene name)|psi-mi:STAT1(display_short)|uniprotkb:Transcription factor ISGF-3 components p91/p84(gene name synonym)	psi-mi:"MI:0424"(protein kinase assay)	Menegazzi et al. (2013)	pubmed:24255956|imex:IM-21760	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8843412|imex:IM-21760-1	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001309133.1|ensembl:ENSG00000096968(gene)|ensembl:ENST00000381652(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0000791"(euchromatin)|go:"GO:0001774"(microglial cell activation)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005131"(growth hormone receptor binding)|go:"GO:0005143"(interleukin-12 receptor binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|ensembl:ENSP00000371067|refseq:NP_004963.1|refseq:NP_001309123.1|refseq:NP_001309124.1|refseq:NP_001309125.1|dip:DIP-33880N|go:"GO:0005730"(nucleolus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005901"(caveola)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0006919"(activation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007167"(enzyme linked receptor protein signaling pathway)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0007259"(receptor signaling pathway via JAK-STAT)|go:"GO:0007260"(tyrosine phosphorylation of STAT protein)|go:"GO:0007498"(mesoderm development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0008631"(intrinsic apoptotic signaling pathway in response to oxidative stress)|go:"GO:0010572"(positive regulation of platelet activation)|go:"GO:0010667"(negative regulation of cardiac muscle cell apoptotic process)|go:"GO:0010811"(positive regulation of cell-substrate adhesion)|go:"GO:0014068"(positive regulation of phosphatidylinositol 3-kinase signaling)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0019221"(cytokine-mediated signaling pathway)|go:"GO:0019901"(protein kinase binding)|go:"GO:0020037"(heme binding)|go:"GO:0022408"(negative regulation of cell-cell adhesion)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030154"(cell differentiation)|go:"GO:0030218"(erythrocyte differentiation)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0031103"(axon regeneration)|go:"GO:0031702"(type 1 angiotensin receptor binding)|go:"GO:0031904"(endosome lumen)|go:"GO:0031959"(mineralocorticoid receptor signaling pathway)|go:"GO:0032024"(positive regulation of insulin secretion)|go:"GO:0032496"(response to lipopolysaccharide)|go:"GO:0032516"(positive regulation of phosphoprotein phosphatase activity)|go:"GO:0032731"(positive regulation of interleukin-1 beta production)|go:"GO:0032760"(positive regulation of tumor necrosis factor production)|go:"GO:0033130"(acetylcholine receptor binding)|go:"GO:0033194"(response to hydroperoxide)|go:"GO:0033209"(tumor necrosis factor-mediated signaling pathway)|go:"GO:0034612"(response to tumor necrosis factor)|go:"GO:0035401"("histone kinase activity (H3-Y41 specific)")|go:"GO:0035409"(histone H3-Y41 phosphorylation)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035722"(interleukin-12-mediated signaling pathway)|go:"GO:0038065"(collagen-activated signaling pathway)|go:"GO:0042169"(SH2 domain binding)|go:"GO:0042307"(positive regulation of protein import into nucleus)|go:"GO:0042393"(histone binding)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042802"(identical protein binding)|go:"GO:0042976"(activation of Janus kinase activity)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043388"(positive regulation of DNA binding)|go:"GO:0043392"(negative regulation of DNA binding)|go:"GO:0043524"(negative regulation of neuron apoptotic process)|go:"GO:0043548"(phosphatidylinositol 3-kinase binding)|go:"GO:0043560"(insulin receptor substrate binding)|go:"GO:0045121"(membrane raft)|go:"GO:0045348"(positive regulation of MHC class II biosynthetic process)|go:"GO:0045428"(regulation of nitric oxide biosynthetic process)|go:"GO:0045429"(positive regulation of nitric oxide biosynthetic process)|go:"GO:0045597"(positive regulation of cell differentiation)|go:"GO:0045822"(negative regulation of heart contraction)|go:"GO:0046425"(regulation of receptor signaling pathway via JAK-STAT)|go:"GO:0046677"(response to antibiotic)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0046872"(metal ion binding)|go:"GO:0048008"(platelet-derived growth factor receptor signaling pathway)|go:"GO:0050727"(regulation of inflammatory response)|go:"GO:0050729"(positive regulation of inflammatory response)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0051428"(peptide hormone receptor binding)|go:"GO:0051770"(positive regulation of nitric-oxide synthase biosynthetic process)|go:"GO:0060333"(interferon-gamma-mediated signaling pathway)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0060391"(positive regulation of SMAD protein signal transduction)|go:"GO:0060396"(growth hormone receptor signaling pathway)|go:"GO:0060397"(growth hormone receptor signaling pathway via JAK-STAT)|go:"GO:0060399"(positive regulation of growth hormone receptor signaling pathway)|go:"GO:0061180"(mammary gland epithelium development)|go:"GO:0070102"(interleukin-6-mediated signaling pathway)|go:"GO:0070671"(response to interleukin-12)|go:"GO:0070757"(interleukin-35-mediated signaling pathway)|go:"GO:0097191"(extrinsic apoptotic signaling pathway)|go:"GO:0097296"(activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway)|go:"GO:0098794"(postsynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099527"(postsynapse to nucleus signaling pathway)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1901731"(positive regulation of platelet aggregation)|go:"GO:1902728"(positive regulation of growth factor dependent skeletal muscle satellite cell proliferation)|go:"GO:1904037"(positive regulation of epithelial cell apoptotic process)|go:"GO:1904707"(positive regulation of vascular associated smooth muscle cell proliferation)|go:"GO:2000273"(positive regulation of signaling receptor activity)|interpro:IPR000299(Band 4.1, N-terminal)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR016251(Tyrosine-protein kinase, Jak/Tyk2)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR019749|interpro:IPR020635|interpro:IPR020693|interpro:IPR035588|interpro:IPR035589|interpro:IPR035860|interpro:IPR035963|interpro:IPR036860|interpro:IPR037838|interpro:IPR041046|interpro:IPR041155|interpro:IPR041381|mint:O60674|rcsb pdb:2B7A|rcsb pdb:2W1I|rcsb pdb:2XA4|rcsb pdb:3E62|rcsb pdb:3E63|rcsb pdb:3E64|rcsb pdb:3FUP|rcsb pdb:3IO7|rcsb pdb:3IOK|rcsb pdb:3JY9|rcsb pdb:3KCK|rcsb pdb:3KRR|rcsb pdb:3LPB|rcsb pdb:3Q32|rcsb pdb:3RVG|rcsb pdb:3TJC|rcsb pdb:3TJD|rcsb pdb:3UGC|rcsb pdb:3ZMM|rcsb pdb:4AQC|rcsb pdb:4BBE|rcsb pdb:4BBF|rcsb pdb:4C61|rcsb pdb:4C62|rcsb pdb:4D0W|rcsb pdb:4D0X|rcsb pdb:4D1S|rcsb pdb:4E4M|rcsb pdb:4E6D|rcsb pdb:4E6Q|rcsb pdb:4F08|rcsb pdb:4F09|rcsb pdb:4FVP|rcsb pdb:4FVQ|rcsb pdb:4FVR|rcsb pdb:4GFM|rcsb pdb:4GMY|rcsb pdb:4HGE|rcsb pdb:4IVA|rcsb pdb:4JI9|rcsb pdb:4JIA|rcsb pdb:4P7E|rcsb pdb:4YTC|rcsb pdb:4YTF|rcsb pdb:4YTH|rcsb pdb:4YTI|rcsb pdb:4Z32|rcsb pdb:4ZIM|rcsb pdb:5CF4|rcsb pdb:5CF5|rcsb pdb:5CF6|rcsb pdb:5CF8|rcsb pdb:5HEZ|rcsb pdb:5I4N|rcsb pdb:5L3A|rcsb pdb:5TQ3|rcsb pdb:5TQ4|rcsb pdb:5TQ5|rcsb pdb:5TQ6|rcsb pdb:5TQ7|rcsb pdb:5TQ8|rcsb pdb:5USY|rcsb pdb:5USZ|rcsb pdb:5UT0|rcsb pdb:5UT1|rcsb pdb:5UT2|rcsb pdb:5UT3|rcsb pdb:5UT4|rcsb pdb:5UT5|rcsb pdb:5UT6|rcsb pdb:5WEV|rcsb pdb:5WIJ|rcsb pdb:5WIK|rcsb pdb:5WIL|rcsb pdb:5WIM|rcsb pdb:5WIN|rcsb pdb:6AAJ|rcsb pdb:6BBV|rcsb pdb:6BRW|rcsb pdb:6BS0|rcsb pdb:6BSS|rcsb pdb:6D2I|rcsb pdb:6DRW|rcsb pdb:6E2P|rcsb pdb:6E2Q|rcsb pdb:6G3C|rcsb pdb:6M9H|rcsb pdb:6OAV|rcsb pdb:6OBB|rcsb pdb:6OBF|rcsb pdb:6OBL|rcsb pdb:6OCC|rcsb pdb:6TPD|rcsb pdb:6VGL|rcsb pdb:6VN8|rcsb pdb:6VNB|rcsb pdb:6VNC|rcsb pdb:6VNE|rcsb pdb:6VNF|rcsb pdb:6VNG|rcsb pdb:6VNH|rcsb pdb:6VNI|rcsb pdb:6VNJ|rcsb pdb:6VNK|rcsb pdb:6VNL|rcsb pdb:6VNM|rcsb pdb:6VS3|rcsb pdb:6VSN|rcsb pdb:6WTN|rcsb pdb:6WTO|rcsb pdb:6WTP|rcsb pdb:6WTQ|rcsb pdb:6X8E|rcsb pdb:6XJK|reactome:R-HSA-1059683|reactome:R-HSA-110056|reactome:R-HSA-112411|reactome:R-HSA-1170546|reactome:R-HSA-1433557|reactome:R-HSA-2586552|reactome:R-HSA-3214858|reactome:R-HSA-512988|reactome:R-HSA-5673000|reactome:R-HSA-5673001|reactome:R-HSA-6785807|reactome:R-HSA-6788467|reactome:R-HSA-6802946|rcsb pdb:5AEP|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-69231|reactome:R-HSA-877300|reactome:R-HSA-877312|reactome:R-HSA-8854691|reactome:R-HSA-8984722|reactome:R-HSA-9006335|reactome:R-HSA-9020591|reactome:R-HSA-9020933|reactome:R-HSA-9020956|reactome:R-HSA-9027276|reactome:R-HSA-9027277|reactome:R-HSA-9027283|reactome:R-HSA-9027284|reactome:R-HSA-912526|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9670439|reactome:R-HSA-9674555|reactome:R-HSA-9679191|reactome:R-HSA-9705462|reactome:R-HSA-982772|reactome:R-HSA-983231	refseq:NP_009330.1|refseq:NP_644671.1|rcsb pdb:1YVL|rcsb pdb:2KA6|rcsb pdb:3WWT|reactome:R-HSA-1059683|reactome:R-HSA-1169408|reactome:R-HSA-1433557|reactome:R-HSA-1839117|reactome:R-HSA-186763|reactome:R-HSA-6785807|reactome:R-HSA-877300|reactome:R-HSA-877312|reactome:R-HSA-8854691|reactome:R-HSA-8939902|reactome:R-HSA-8984722|reactome:R-HSA-8985947|reactome:R-HSA-9013508|reactome:R-HSA-9020956|reactome:R-HSA-9020958|reactome:R-HSA-909733|rcsb pdb:1BF5|reactome:R-HSA-9670439|reactome:R-HSA-9673767|reactome:R-HSA-9673770|reactome:R-HSA-9674555|reactome:R-HSA-9705462|reactome:R-HSA-982772|reactome:R-HSA-912694|refseq:XP_006712781.1|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005164"(tumor necrosis factor receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006952"(defense response)|go:"GO:0007259"(receptor signaling pathway via JAK-STAT)|go:"GO:0007584"(response to nutrient)|go:"GO:0008015"(blood circulation)|go:"GO:0009612"(response to mechanical stimulus)|go:"GO:0010742"(macrophage derived foam cell differentiation)|go:"GO:0016525"(negative regulation of angiogenesis)|go:"GO:0016922"(nuclear receptor binding)|ensembl:ENSG00000115415(gene)|ensembl:ENST00000361099(transcript)|ensembl:ENST00000409465(transcript)|ensembl:ENST00000415035(transcript)|ensembl:ENST00000540176(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000785"(chromatin)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000979"(RNA polymerase II core promoter sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001937"(negative regulation of endothelial cell proliferation)|go:"GO:0002053"(positive regulation of mesenchymal cell proliferation)|go:"GO:0002230"(positive regulation of defense response to virus by host)|go:"GO:0003340"(negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis)|go:"GO:0003690"(double-stranded DNA binding)|go:"GO:0042493"|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0042802"(identical protein binding)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043124"(negative regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0043434"(response to peptide hormone)|go:"GO:0044389"(ubiquitin-like protein ligase binding)|go:"GO:0045296"(cadherin binding)|go:"GO:0045648"(positive regulation of erythrocyte differentiation)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0072162"(metanephric mesenchymal cell differentiation)|go:"GO:0072308"(negative regulation of metanephric nephron tubule epithelial cell differentiation)|go:"GO:0140297"(DNA-binding transcription factor binding)|go:"GO:1990841"(promoter-specific chromatin binding)|interpro:IPR000980(SH2 motif)|interpro:IPR001217(STAT transcription factor, core)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|interpro:IPR008967(p53-like transcription factor, DNA-binding)|go:"GO:0046725"(negative regulation by virus of viral protein levels in host cell)|interpro:IPR012345(STAT transcription factor, DNA-binding, subdomain)|interpro:IPR013799(STAT transcription factor, protein interaction)|interpro:IPR013800(STAT transcription factor, all-alpha)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048661"(positive regulation of smooth muscle cell proliferation)|go:"GO:0051591"(response to cAMP)|go:"GO:0051607"(defense response to virus)|go:"GO:0051721"(protein phosphatase 2A binding)|go:"GO:0051770"(positive regulation of nitric-oxide synthase biosynthetic process)|go:"GO:0060333"(interferon-gamma-mediated signaling pathway)|go:"GO:0060337"(type I interferon signaling pathway)|go:"GO:0061326"(renal tubule development)|go:"GO:0070106"(interleukin-27-mediated signaling pathway)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071407"(cellular response to organic cyclic compound)|interpro:IPR013801(STAT transcription factor, DNA-binding)|interpro:IPR015988(STAT transcription factor, coiled coil)|interpro:IPR022752|go:"GO:0072136"(metanephric mesenchymal cell proliferation involved in metanephros development)|interpro:IPR035859|interpro:IPR036535|interpro:IPR036860|interpro:IPR038295|mint:P42224|go:"GO:0019221"(cytokine-mediated signaling pathway)|go:"GO:0019899"(enzyme binding)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0031730"(CCR5 chemokine receptor binding)|go:"GO:0032727"(positive regulation of interferon-alpha production)|go:"GO:0032869"(cellular response to insulin stimulus)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033209"(tumor necrosis factor-mediated signaling pathway)|go:"GO:0034097"(response to cytokine)|go:"GO:0035035"(histone acetyltransferase binding)|go:"GO:0035456"(response to interferon-beta)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0042393"(histone binding)|dip:DIP-46140N	-	-	-	figure legend:f5 sf3|comment:"To demonstrate that the direct interaction occurring between active catechins and STAT1 is one of  the principal molecular mechanisms of STAT1 Tyr701 phosphorylation inhibition, a cell-free  kinase in vitro assay was performed. Recombinant STAT1, pre-incubated with active catechins,  were mixed with recombinant active JAK2. Western blot shows the ability of anti-STAT1 catechins  to reduce, in a dose-dependent manner, in vitro STAT1 phosphorylation (Figure 5). In line with our  hypothesis, we also demonstrated that the inactive compounds were not able to prevent STAT1  phosphorylation (data not shown)."|comment:Finally, cell free JAK2-kinase assays support SPR and docking data, and confirm that the direct  binding of active catechins with STAT1 protein is an important event to affect Tyr701  phosphorylation. Indeed, in vitro kinase assay data indicate that wtSTAT1-catechins pre-treatmentwas able to inhibit, in a dose dependent manner, STAT1 Tyr701 phosphorylation otherwise these  effect was not observed with H568A-STAT1 protein|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/05	2014/10/16	rogid:s2LnvkFxriuFzkRSlP9PI0h/wYk9606	rogid:a2ZnhdFZkctbjaXLpsYNJ3q5Cv49606	intact-crc:297D5F67338423F6|rigid:LjVP2xvsRfM2JxLgPKiWvqRwS30	false	N-phosphorylated residue:1008-1008	phosphorylated residue:701-701	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)