#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q12840	uniprotkb:Q9UPV9	intact:EBI-713468|uniprotkb:Q4LE26|ensembl:ENSP00000408979|uniprotkb:A6H8M5	intact:EBI-1105048|ensembl:ENSP00000328998|uniprotkb:Q96B69|uniprotkb:Q63HR0|uniprotkb:E9PDS2|uniprotkb:Q659B5|uniprotkb:J3KNT7	psi-mi:kif5a_human(display_long)|uniprotkb:Neuronal kinesin heavy chain(gene name synonym)|uniprotkb:Kinesin heavy chain neuron-specific 1(gene name synonym)|uniprotkb:KIF5A(gene name)|psi-mi:KIF5A(display_short)|uniprotkb:NKHC1(gene name synonym)	psi-mi:trak1_human(display_long)|uniprotkb:Protein Milton(gene name synonym)|uniprotkb:TRAK1(gene name)|psi-mi:TRAK1(display_short)|uniprotkb:KIAA1042(gene name synonym)|uniprotkb:OIP106(gene name synonym)|uniprotkb:106 kDa O-GlcNAc transferase-interacting protein(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Randall et al. (2013)	pubmed:24161670|imex:IM-21706	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8786097|imex:IM-21706-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENST00000455537(transcript)|go:"GO:0003774"(cytoskeletal motor activity)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0016020"(membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1990049"(retrograde neuronal dense core vesicle transport)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:Q12840|rcsb pdb:4UXT|rcsb pdb:4UXY|rcsb pdb:4UY0|reactome:R-HSA-2132295|reactome:R-HSA-264876|reactome:R-HSA-5625970|reactome:R-HSA-6811434|reactome:R-HSA-983189|ensembl:ENSG00000155980(gene)|refseq:NP_004975.2|dip:DIP-37584N	ensembl:ENSG00000182606(gene)|ensembl:ENST00000327628(transcript)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005829"(cytosol)|go:"GO:0005938"(cell cortex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006493"(protein O-linked glycosylation)|go:"GO:0006605"(protein targeting)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0017022"(myosin binding)|go:"GO:0022008"(neurogenesis)|go:"GO:0030911"(TPR domain binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0044295"(axonal growth cone)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048311"(mitochondrion distribution)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0050811"(GABA receptor binding)|go:"GO:0098957"(anterograde axonal transport of mitochondrion)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR006933(HAP1, N-terminal)|interpro:IPR022154|mint:Q9UPV9|reactome:R-HSA-6802952|reactome:R-HSA-9013419|reactome:R-HSA-9013425|go:"GO:0030425"(dendrite)|refseq:NP_001252537.1|refseq:NP_055780.2|refseq:NP_001252539.1|refseq:NP_001036111.1	-	-	-	figure legend:f5|comment:"Thus using this approach, three regions within the KIF5A Cterminal  region were shown to contribute to the TRAK2 binding site i.e. KIF5A 942-961; KIF5A 877-  885 and KIF5A 861-877. (Note that full immunoblots for Figures 3 – 5 can be viewed in the  Supplementary Information, Figures S1- S3).  The region KIF5A 877-883 was studied in more detail. Three additional truncations were made at  KIF5A 883, KIF5A 881 and KIF5A 879 plus the region 877- 883 was deleted. The constructs  truncated at KIF5A 883, KIF5A 881 and KIF5A 879 co-immunoprecipitated with TRAK2 with a  similar efficiency to KIF5A1-885 as indeed did the KIF5AΔ877-883 deletion. The latter was unexpected  since this region, amino acid sequence GALKEAKE, had been clearly implicated in the efficiency of  KIF5A/TRAK2 association (Figure 3D). It may be explained by an alteration of the KIF5A tertiary  structure such that other amino acids requisite for the binding of TRAK2 are now available to  compensate for the deleted region thus the predicted loss in efficacy of co-immunoprecipitation is  circumvented. In the context of the full length KIF5A therefore, the other sites of interaction are  sufficient to support partial association with TRAK2 even when 877-883 is removed."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293a)|taxid:9606(Homo sapiens)	-	2013/10/02	2014/10/16	rogid:fvhe2vZFe1vN8SS4IPCMA5Nr7j09606	rogid:wO1gttlJMJ/4RvsellzkZ2YHGiE9606	intact-crc:1FEDC5EB93F8E3B9|rigid:cIqyqc2+eXikg0sPylhDmXXTS5s	false	his tag:?-?|sufficient binding region:825-877	myc tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8R2H7	uniprotkb:Q12840	intact:EBI-1396483|uniprotkb:Q8R2H6|uniprotkb:Q8R4G3|ensembl:ENSRNOP00000014939|ensembl:ENSRNOP00000015035	intact:EBI-713468|uniprotkb:Q4LE26|ensembl:ENSP00000408979|uniprotkb:A6H8M5	psi-mi:trak2_rat(display_long)|uniprotkb:Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein homolog(gene name synonym)|uniprotkb:GABA-A receptor-interacting factor 1(gene name synonym)|uniprotkb:O-GlcNAc transferase-interacting protein of 98 kDa(gene name synonym)|uniprotkb:Trak2(gene name)|psi-mi:Trak2(display_short)|uniprotkb:Als2cr3(gene name synonym)|uniprotkb:Grif1(gene name synonym)|uniprotkb:Oip98(gene name synonym)	psi-mi:kif5a_human(display_long)|uniprotkb:Neuronal kinesin heavy chain(gene name synonym)|uniprotkb:Kinesin heavy chain neuron-specific 1(gene name synonym)|uniprotkb:KIF5A(gene name)|psi-mi:KIF5A(display_short)|uniprotkb:NKHC1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Randall et al. (2013)	pubmed:24161670|imex:IM-21706	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8785998|imex:IM-21706-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:620915|refseq:NP_598244.2|ensembl:ENSRNOG00000010881(gene)|ensembl:ENSRNOT00000014939(transcript)|ensembl:ENSRNOT00000015034(transcript)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006493"(protein O-linked glycosylation)|go:"GO:0006605"(protein targeting)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0017022"(myosin binding)|go:"GO:0019894"(kinesin binding)|go:"GO:0019899"(enzyme binding)|go:"GO:0022008"(neurogenesis)|go:"GO:0030425"(dendrite)|go:"GO:0030911"(TPR domain binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044295"(axonal growth cone)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048311"(mitochondrion distribution)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0050771"(negative regulation of axonogenesis)|go:"GO:0050811"(GABA receptor binding)|go:"GO:0098939"(dendritic transport of mitochondrion)|go:"GO:0098957"(anterograde axonal transport of mitochondrion)|go:"GO:0098972"(anterograde dendritic transport of mitochondrion)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR006933(HAP1, N-terminal)|interpro:IPR022154|mint:Q8R2H7|reactome:R-RNO-9013419|reactome:R-RNO-9013425	ensembl:ENST00000455537(transcript)|go:"GO:0003774"(cytoskeletal motor activity)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0016020"(membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1990049"(retrograde neuronal dense core vesicle transport)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:Q12840|rcsb pdb:4UXT|rcsb pdb:4UXY|rcsb pdb:4UY0|reactome:R-HSA-2132295|reactome:R-HSA-264876|reactome:R-HSA-5625970|reactome:R-HSA-6811434|reactome:R-HSA-983189|ensembl:ENSG00000155980(gene)|refseq:NP_004975.2|dip:DIP-37584N	-	-	-	figure legend:f2 f3|comment:"Thus using this approach, three regions within the KIF5A Cterminal  region were shown to contribute to the TRAK2 binding site i.e. KIF5A 942-961; KIF5A 877-  885 and KIF5A 861-877. (Note that full immunoblots for Figures 3 – 5 can be viewed in the  Supplementary Information, Figures S1- S3).  The region KIF5A 877-883 was studied in more detail. Three additional truncations were made at  KIF5A 883, KIF5A 881 and KIF5A 879 plus the region 877- 883 was deleted. The constructs  truncated at KIF5A 883, KIF5A 881 and KIF5A 879 co-immunoprecipitated with TRAK2 with a  similar efficiency to KIF5A1-885 as indeed did the KIF5AΔ877-883 deletion. The latter was unexpected  since this region, amino acid sequence GALKEAKE, had been clearly implicated in the efficiency of  KIF5A/TRAK2 association (Figure 3D). It may be explained by an alteration of the KIF5A tertiary  structure such that other amino acids requisite for the binding of TRAK2 are now available to  compensate for the deleted region thus the predicted loss in efficacy of co-immunoprecipitation is  circumvented. In the context of the full length KIF5A therefore, the other sites of interaction are  sufficient to support partial association with TRAK2 even when 877-883 is removed."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293a)|taxid:9606(Homo sapiens)	-	2013/10/02	2014/10/16	rogid:bE/j/R8zWCiVsk2VUElznceSmB810116	rogid:fvhe2vZFe1vN8SS4IPCMA5Nr7j09606	intact-crc:8186774CCFDF01D2|rigid:hlVBENCn+4W1FTSZ8PY4+W7Ov0E	false	flag tag:?-?	sufficient binding region:942-961|his tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8R2H7	uniprotkb:Q12840	intact:EBI-1396483|uniprotkb:Q8R2H6|uniprotkb:Q8R4G3|ensembl:ENSRNOP00000014939|ensembl:ENSRNOP00000015035	intact:EBI-713468|uniprotkb:Q4LE26|ensembl:ENSP00000408979|uniprotkb:A6H8M5	psi-mi:trak2_rat(display_long)|uniprotkb:Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein homolog(gene name synonym)|uniprotkb:GABA-A receptor-interacting factor 1(gene name synonym)|uniprotkb:O-GlcNAc transferase-interacting protein of 98 kDa(gene name synonym)|uniprotkb:Trak2(gene name)|psi-mi:Trak2(display_short)|uniprotkb:Als2cr3(gene name synonym)|uniprotkb:Grif1(gene name synonym)|uniprotkb:Oip98(gene name synonym)	psi-mi:kif5a_human(display_long)|uniprotkb:Neuronal kinesin heavy chain(gene name synonym)|uniprotkb:Kinesin heavy chain neuron-specific 1(gene name synonym)|uniprotkb:KIF5A(gene name)|psi-mi:KIF5A(display_short)|uniprotkb:NKHC1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Randall et al. (2013)	pubmed:24161670|imex:IM-21706	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8786022|imex:IM-21706-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:620915|refseq:NP_598244.2|ensembl:ENSRNOG00000010881(gene)|ensembl:ENSRNOT00000014939(transcript)|ensembl:ENSRNOT00000015034(transcript)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006493"(protein O-linked glycosylation)|go:"GO:0006605"(protein targeting)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0017022"(myosin binding)|go:"GO:0019894"(kinesin binding)|go:"GO:0019899"(enzyme binding)|go:"GO:0022008"(neurogenesis)|go:"GO:0030425"(dendrite)|go:"GO:0030911"(TPR domain binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044295"(axonal growth cone)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048311"(mitochondrion distribution)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0050771"(negative regulation of axonogenesis)|go:"GO:0050811"(GABA receptor binding)|go:"GO:0098939"(dendritic transport of mitochondrion)|go:"GO:0098957"(anterograde axonal transport of mitochondrion)|go:"GO:0098972"(anterograde dendritic transport of mitochondrion)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR006933(HAP1, N-terminal)|interpro:IPR022154|mint:Q8R2H7|reactome:R-RNO-9013419|reactome:R-RNO-9013425	ensembl:ENST00000455537(transcript)|go:"GO:0003774"(cytoskeletal motor activity)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0016020"(membrane)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0019894"(kinesin binding)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:1904115"(axon cytoplasm)|go:"GO:1990049"(retrograde neuronal dense core vesicle transport)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|interpro:IPR027640|interpro:IPR036961|mint:Q12840|rcsb pdb:4UXT|rcsb pdb:4UXY|rcsb pdb:4UY0|reactome:R-HSA-2132295|reactome:R-HSA-264876|reactome:R-HSA-5625970|reactome:R-HSA-6811434|reactome:R-HSA-983189|ensembl:ENSG00000155980(gene)|refseq:NP_004975.2|dip:DIP-37584N	-	-	-	figure legend:f2 f3|comment:"Thus using this approach, three regions within the KIF5A Cterminal  region were shown to contribute to the TRAK2 binding site i.e. KIF5A 942-961; KIF5A 877-  885 and KIF5A 861-877. (Note that full immunoblots for Figures 3 – 5 can be viewed in the  Supplementary Information, Figures S1- S3).  The region KIF5A 877-883 was studied in more detail. Three additional truncations were made at  KIF5A 883, KIF5A 881 and KIF5A 879 plus the region 877- 883 was deleted. The constructs  truncated at KIF5A 883, KIF5A 881 and KIF5A 879 co-immunoprecipitated with TRAK2 with a  similar efficiency to KIF5A1-885 as indeed did the KIF5AΔ877-883 deletion. The latter was unexpected  since this region, amino acid sequence GALKEAKE, had been clearly implicated in the efficiency of  KIF5A/TRAK2 association (Figure 3D). It may be explained by an alteration of the KIF5A tertiary  structure such that other amino acids requisite for the binding of TRAK2 are now available to  compensate for the deleted region thus the predicted loss in efficacy of co-immunoprecipitation is  circumvented. In the context of the full length KIF5A therefore, the other sites of interaction are  sufficient to support partial association with TRAK2 even when 877-883 is removed."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293a)|taxid:9606(Homo sapiens)	-	2013/10/02	2014/10/16	rogid:bE/j/R8zWCiVsk2VUElznceSmB810116	rogid:fvhe2vZFe1vN8SS4IPCMA5Nr7j09606	intact-crc:0324DF3686DE8ED0|rigid:hlVBENCn+4W1FTSZ8PY4+W7Ov0E	false	flag tag:?-?	sufficient binding region:861-877|his tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q8R2H7	uniprotkb:O60282	intact:EBI-1396483|uniprotkb:Q8R2H6|uniprotkb:Q8R4G3|ensembl:ENSRNOP00000014939|ensembl:ENSRNOP00000015035	intact:EBI-717170|uniprotkb:O95079|uniprotkb:Q2YDC5|ensembl:ENSP00000393379|ensembl:ENSP00000503013	psi-mi:trak2_rat(display_long)|uniprotkb:Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein homolog(gene name synonym)|uniprotkb:GABA-A receptor-interacting factor 1(gene name synonym)|uniprotkb:O-GlcNAc transferase-interacting protein of 98 kDa(gene name synonym)|uniprotkb:Trak2(gene name)|psi-mi:Trak2(display_short)|uniprotkb:Als2cr3(gene name synonym)|uniprotkb:Grif1(gene name synonym)|uniprotkb:Oip98(gene name synonym)	psi-mi:kif5c_human(display_long)|uniprotkb:Kinesin heavy chain neuron-specific 2(gene name synonym)|uniprotkb:KIF5C(gene name)|psi-mi:KIF5C(display_short)|uniprotkb:KIAA0531(gene name synonym)|uniprotkb:NKHC2(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Randall et al. (2013)	pubmed:24161670|imex:IM-21706	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8786036|imex:IM-21706-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:620915|refseq:NP_598244.2|ensembl:ENSRNOG00000010881(gene)|ensembl:ENSRNOT00000014939(transcript)|ensembl:ENSRNOT00000015034(transcript)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005886"(plasma membrane)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006493"(protein O-linked glycosylation)|go:"GO:0006605"(protein targeting)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0008333"(endosome to lysosome transport)|go:"GO:0017022"(myosin binding)|go:"GO:0019894"(kinesin binding)|go:"GO:0019899"(enzyme binding)|go:"GO:0022008"(neurogenesis)|go:"GO:0030425"(dendrite)|go:"GO:0030911"(TPR domain binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0043005"(neuron projection)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044295"(axonal growth cone)|go:"GO:0047496"(vesicle transport along microtubule)|go:"GO:0048311"(mitochondrion distribution)|go:"GO:0048813"(dendrite morphogenesis)|go:"GO:0050771"(negative regulation of axonogenesis)|go:"GO:0050811"(GABA receptor binding)|go:"GO:0098939"(dendritic transport of mitochondrion)|go:"GO:0098957"(anterograde axonal transport of mitochondrion)|go:"GO:0098972"(anterograde dendritic transport of mitochondrion)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR006933(HAP1, N-terminal)|interpro:IPR022154|mint:Q8R2H7|reactome:R-RNO-9013419|reactome:R-RNO-9013425	refseq:NP_004513.1|refseq:XP_011509459.1|refseq:XP_016859551.1|ensembl:ENSG00000168280(gene)|ensembl:ENST00000435030(transcript)|ensembl:ENST00000677891(transcript)|go:"GO:0003777"(microtubule motor activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005871"(kinesin complex)|go:"GO:0005874"(microtubule)|go:"GO:0006996"(organelle organization)|go:"GO:0007018"(microtubule-based movement)|go:"GO:0007411"(axon guidance)|go:"GO:0008017"(microtubule binding)|go:"GO:0008045"(motor neuron axon guidance)|go:"GO:0008574"(plus-end-directed microtubule motor activity)|go:"GO:0030705"(cytoskeleton-dependent intracellular transport)|go:"GO:0032839"(dendrite cytoplasm)|go:"GO:0035253"(ciliary rootlet)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044295"(axonal growth cone)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0051028"(mRNA transport)|go:"GO:0098971"(anterograde dendritic transport of neurotransmitter receptor complex)|go:"GO:0099641"(anterograde axonal protein transport)|go:"GO:0150034"(distal axon)|go:"GO:1904115"(axon cytoplasm)|interpro:IPR001752(Kinesin, motor region)|interpro:IPR019821|interpro:IPR027417|mint:O60282|reactome:R-HSA-264876|interpro:IPR036961	-	-	-	figure legend:f4|comment:"Thus using this approach, three regions within the KIF5A Cterminal  region were shown to contribute to the TRAK2 binding site i.e. KIF5A 942-961; KIF5A 877-  885 and KIF5A 861-877. (Note that full immunoblots for Figures 3 – 5 can be viewed in the  Supplementary Information, Figures S1- S3).  The region KIF5A 877-883 was studied in more detail. Three additional truncations were made at  KIF5A 883, KIF5A 881 and KIF5A 879 plus the region 877- 883 was deleted. The constructs  truncated at KIF5A 883, KIF5A 881 and KIF5A 879 co-immunoprecipitated with TRAK2 with a  similar efficiency to KIF5A1-885 as indeed did the KIF5AΔ877-883 deletion. The latter was unexpected  since this region, amino acid sequence GALKEAKE, had been clearly implicated in the efficiency of  KIF5A/TRAK2 association (Figure 3D). It may be explained by an alteration of the KIF5A tertiary  structure such that other amino acids requisite for the binding of TRAK2 are now available to  compensate for the deleted region thus the predicted loss in efficacy of co-immunoprecipitation is  circumvented. In the context of the full length KIF5A therefore, the other sites of interaction are  sufficient to support partial association with TRAK2 even when 877-883 is removed."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293a)|taxid:9606(Homo sapiens)	-	2013/10/02	2014/10/16	rogid:bE/j/R8zWCiVsk2VUElznceSmB810116	rogid:4TqyBBhm1/nyaCSyz66oisPiwpA9606	intact-crc:676E7DE4ABBB70FE|rigid:Vge7m3aUPJWaglwwF2Oqag7BmAY	false	flag tag:?-?	sufficient binding region:828-881	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)