#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P00669	uniprotkb:P00669	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Sica et al. (2013)	imex:IM-21651|pubmed:24140346	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8789046|imex:IM-21651-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	-	comment:mint	comment:mint	figure legend:f1|comment:"line). Peak integration revealed that more than 60% of the protein is  eluted as monomer, whilst the remaining part corresponds to aggregated forms of different size, although the  relative amount of each aggregate varied slightly with the elution buffer. From the elution volume it is possible  to calculate that the peak indicated as TrART corresponds to the size of a trimer, and the one indicated as DART  to the size of a dimer. The trimer and dimer amounts are equivalent roughly to the 10% and 20-25% of the total  protein amount respectively."|comment:"A similar experiment was performed on the STAA-mBS mutant, obtaining the chromatographic profile also  reported in Figure 1 (solid line), which closely resembles the one obtained with the parent (wild-type) protein,  except for the slightly higher amount of aggregates. Very similar results were obtained with other BS-RNase  mutants, in which residues having key roles in the swapping phenomenon were substituted with the  corresponding ones of RNase A and which have a different behaviour with respect to the swapping of the N5  terminal ends, i.e.R80S-STAA-mBS [27] and PALQ-mBS [28]. The elution profile of PALQ-mBS is also shown  in Fig. 1 as an example (dotted line)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/07	2014/10/16	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	intact-crc:1E0198ABF770C58B|rigid:FpgFHDcuikcFlyZ+k7jNR8dsNn8	false	-	-	3	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P00669	uniprotkb:P00669	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Sica et al. (2013)	imex:IM-21651|pubmed:24140346	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8788995|imex:IM-21651-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	-	comment:mint	comment:mint	figure legend:f1|comment:"line). Peak integration revealed that more than 60% of the protein is  eluted as monomer, whilst the remaining part corresponds to aggregated forms of different size, although the  relative amount of each aggregate varied slightly with the elution buffer. From the elution volume it is possible  to calculate that the peak indicated as TrART corresponds to the size of a trimer, and the one indicated as DART  to the size of a dimer. The trimer and dimer amounts are equivalent roughly to the 10% and 20-25% of the total  protein amount respectively."|comment:"A similar experiment was performed on the STAA-mBS mutant, obtaining the chromatographic profile also  reported in Figure 1 (solid line), which closely resembles the one obtained with the parent (wild-type) protein,  except for the slightly higher amount of aggregates. Very similar results were obtained with other BS-RNase  mutants, in which residues having key roles in the swapping phenomenon were substituted with the  corresponding ones of RNase A and which have a different behaviour with respect to the swapping of the N5  terminal ends, i.e.R80S-STAA-mBS [27] and PALQ-mBS [28]. The elution profile of PALQ-mBS is also shown  in Fig. 1 as an example (dotted line)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/07	2014/10/16	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	intact-crc:183E25551811C58B|rigid:FpgFHDcuikcFlyZ+k7jNR8dsNn8	false	-	-	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P00669	uniprotkb:P00669	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:"MI:0276"(blue native page)	Sica et al. (2013)	imex:IM-21651|pubmed:24140346	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8789065|imex:IM-21651-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	-	comment:mint	comment:mint	figure legend:f2|comment:"The analysis of the gel indicated clearly  that BS-RNase and NCD-BS (lanes 3 and 4) are characterized by a single band. On the contrary, both the  artificial dimers (lanes 5 and 6) showed the presence of a significant amount of the corresponding monomeric  forms (lanes 1 and 2), which necessarily had to be produced upon dissociation of the dimeric species after the  gel-filtration and even while running the gel. Interestingly, the comparison of lanes 3 and 4 with 5 and 6  indicates that the artificial dimers migrate on the gel with a mobility very close to that of NCD-BS, thus  suggesting the presence of a single dimeric form even in the samples prepared by acetic acid lyophilisation, in  contrast with the results obtained for RNase A. It is indeed well known that the two dimers formed when  RNase A is subjected to the same treatment can be separated by cathodic gel-electrophoresis [36]."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/07	2014/10/16	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	intact-crc:E33D8A596373D492|rigid:FpgFHDcuikcFlyZ+k7jNR8dsNn8	false	-	-	2	0	psi-mi:"MI:0816"(molecular weight estimation by staining)	psi-mi:"MI:0816"(molecular weight estimation by staining)
uniprotkb:P00669	uniprotkb:P00669	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	intact:EBI-8524799|intact:MINT-8050403|ensembl:ENSBTAP00000036091	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:rns_bovin(display_long)|uniprotkb:SRN(gene name)|psi-mi:SRN(display_short)|uniprotkb:Ribonuclease BS-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Sica et al. (2013)	imex:IM-21651|pubmed:24140346	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8789100|imex:IM-21651-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	refseq:NP_861526.1|ensembl:ENSBTAG00000025663(gene)|ensembl:ENSBTAT00000036229(transcript)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0004522"(ribonuclease A activity)|go:"GO:0004540"(ribonuclease activity)|go:"GO:0005576"(extracellular region)|go:"GO:0016829"(lyase activity)|go:"GO:0042802"(identical protein binding)|go:"GO:0090501"(RNA phosphodiester bond hydrolysis)|interpro:IPR001427(Pancreatic ribonuclease)|interpro:IPR023411|interpro:IPR023412|interpro:IPR036816|mint:P00669|rcsb pdb:11BA|rcsb pdb:11BG|rcsb pdb:1BSR|rcsb pdb:1N1X|rcsb pdb:1N3Z|rcsb pdb:1QWQ|rcsb pdb:1R3M|rcsb pdb:1R5C|rcsb pdb:1R5D|rcsb pdb:1TQ9|rcsb pdb:1Y92|rcsb pdb:1Y94|rcsb pdb:2LFJ|rcsb pdb:3BCM|rcsb pdb:3BCO|rcsb pdb:3BCP|rcsb pdb:3DJP|rcsb pdb:3DJQ|rcsb pdb:3DJV|rcsb pdb:3DJX|rcsb pdb:4N4C|rcsb pdb:3DJO	-	comment:mint	comment:mint	figure legend:f3 f4 t1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/07	2014/10/16	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	rogid:9//PWBK3cYNm/GbB26FJzuRWsK09913	intact-crc:E96A7DC0C81A08C9|rigid:FpgFHDcuikcFlyZ+k7jNR8dsNn8	false	-	-	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)