#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P02699	uniprotkb:P04695	intact:EBI-8592832|ensembl:ENSBTAP00000001730|intact:MINT-1507462	intact:EBI-7052221|ensembl:ENSBTAP00000023990|intact:UNK-6021598	psi-mi:opsd_bovin(display_long)|uniprotkb:RHO(gene name)|psi-mi:RHO(display_short)	psi-mi:gnat1_bovin(display_long)|uniprotkb:GNAT1(gene name)|psi-mi:GNAT1(display_short)|uniprotkb:Transducin alpha-1 chain(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Singhal et al. (2013)	pubmed:23579341|imex:IM-21797	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8871547|imex:IM-21797-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSBTAG00000001310(gene)|ensembl:ENSBTAT00000001730(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0001965"(G-protein alpha-subunit binding)|go:"GO:0002046"(opsin binding)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005502"(11-cis retinal binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007601"(visual perception)|go:"GO:0007602"(phototransduction)|go:"GO:0007603"(phototransduction, visible light)|go:"GO:0008020"(G protein-coupled photoreceptor activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009416"(response to light stimulus)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016038"(absorption of visible light)|go:"GO:0016056"(rhodopsin mediated signaling pathway)|go:"GO:0018298"(protein-chromophore linkage)|go:"GO:0019867"(outer membrane)|go:"GO:0042622"(photoreceptor outer segment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043052"(thermotaxis)|go:"GO:0045494"(photoreceptor cell maintenance)|go:"GO:0050960"(detection of temperature stimulus involved in thermoception)|go:"GO:0060041"(retina development in camera-type eye)|go:"GO:0060342"(photoreceptor inner segment membrane)|go:"GO:0071482"(cellular response to light stimulus)|go:"GO:0097225"(sperm midpiece)|go:"GO:0097381"(photoreceptor disc membrane)|go:"GO:0097648"(G protein-coupled receptor complex)|go:"GO:1990763"(arrestin family protein binding)|go:"GO:1990913"(sperm head plasma membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000732(Rhodopsin)|interpro:IPR001760(Opsin)|rcsb pdb:1NZS|rcsb pdb:1OV0|rcsb pdb:1OV1|rcsb pdb:1U19|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1VQX|rcsb pdb:2G87|rcsb pdb:2HPY|interpro:IPR019477|rcsb pdb:2I35|interpro:IPR027430|mint:P02699|rcsb pdb:1BOJ|rcsb pdb:1BOK|rcsb pdb:1EDS|rcsb pdb:1EDV|rcsb pdb:1EDW|rcsb pdb:2I36|rcsb pdb:2I37|rcsb pdb:2IQK|rcsb pdb:2J4Y|rcsb pdb:1EDX|rcsb pdb:2PED|rcsb pdb:2X72|rcsb pdb:3C9L|rcsb pdb:3C9M|rcsb pdb:3CAP|rcsb pdb:3DQB|rcsb pdb:3OAX|rcsb pdb:3PQR|rcsb pdb:3PXO|rcsb pdb:4A4M|rcsb pdb:4BEY|rcsb pdb:1F88|rcsb pdb:4BEZ|rcsb pdb:1FDF|rcsb pdb:1GZM|rcsb pdb:4J4Q|rcsb pdb:4PXF|rcsb pdb:4X1H|rcsb pdb:5DYS|rcsb pdb:5EN0|rcsb pdb:5TE3|rcsb pdb:5TE5|rcsb pdb:5WKT|rcsb pdb:6FK6|rcsb pdb:6FK7|rcsb pdb:6FK8|rcsb pdb:6FK9|rcsb pdb:6FKA|rcsb pdb:6FKB|rcsb pdb:6FKC|rcsb pdb:6FKD|rcsb pdb:6FUF|rcsb pdb:6NWE|rcsb pdb:6OFJ|rcsb pdb:6OY9|rcsb pdb:6OYA|rcsb pdb:6PEL|rcsb pdb:6PGS|rcsb pdb:6PH7|rcsb pdb:6QNO|reactome:R-BTA-2453902|reactome:R-BTA-2485179|reactome:R-BTA-2514859|reactome:R-BTA-418594|reactome:R-BTA-419771|reactome:R-BTA-5620916|rcsb pdb:1HZX|rcsb pdb:1JFP|rcsb pdb:1L9H|rcsb pdb:1LN6|rcsb pdb:1N3M|refseq:NP_001014890.1|dip:DIP-29225N	ensembl:ENSBTAG00000018020(gene)|ensembl:ENSBTAT00000023990(transcript)|go:"GO:0000035"(acyl binding)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0001917"(photoreceptor inner segment)|go:"GO:0001973"(G protein-coupled adenosine receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0007193"(adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway)|go:"GO:0007602"(phototransduction)|go:"GO:0007603"(phototransduction, visible light)|go:"GO:0008283"(cell population proliferation)|go:"GO:0009416"(response to light stimulus)|go:"GO:0019003"(GDP binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0032391"(photoreceptor connecting cilium)|go:"GO:0042462"(eye photoreceptor cell development)|go:"GO:0042622"(photoreceptor outer segment membrane)|go:"GO:0046872"(metal ion binding)|go:"GO:0050908"(detection of light stimulus involved in visual perception)|go:"GO:0050917"(sensory perception of umami taste)|go:"GO:0051344"(negative regulation of cyclic-nucleotide phosphodiesterase activity)|go:"GO:0060041"(retina development in camera-type eye)|go:"GO:0071257"(cellular response to electrical stimulus)|go:"GO:0097381"(photoreceptor disc membrane)|go:"GO:0097648"(G protein-coupled receptor complex)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR001408(G-protein alpha subunit, group I)|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:3V00|rcsb pdb:4BEY|mint:P04695|rcsb pdb:4J4Q|rcsb pdb:6OY9|rcsb pdb:1AQG|rcsb pdb:6OYA|rcsb pdb:7JSN|reactome:R-BTA-2514859|reactome:R-BTA-418594|rcsb pdb:1FQJ|rcsb pdb:1FQK|rcsb pdb:1GOT|rcsb pdb:1LVZ|rcsb pdb:1TAD|rcsb pdb:1TAG|rcsb pdb:1TND|rcsb pdb:2X72|rcsb pdb:3DQB|rcsb pdb:3PQR|refseq:XP_010815966.1|refseq:NP_851365.1|dip:DIP-29226N	rcsb pdb:4bey	comment:mint|function:Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal	function:"Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase"|comment:mint	figure legend:st1 f1 f3|comment:"We obtained crystals  of G90D opsin, light-activated G90D and G90D-GaCT2, which  diffracted to 3.9, 3.3 and 2.9A°  , respectively (supplementary Table  S1 online). No crystals of the G90D dark state could be grown.  The presence of retinal and the GaCT2 peptide both increased  diffraction quality, consistent with their ability to bind and  stabilize an active conformation of the receptor."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/22	2014/10/16	rogid:ntvPNbJU0fcn+gajwUZzvfd/YCI9913	rogid:sQakZVwi5BGM6vu3X/xrS6R5QAk9913	intact-crc:63ACF3A9A39B12C2|rigid:RhiHFcmpa8JqycB5R+fzUDBh55o	false	-	binding-associated region:340-350	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)