#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q46705	uniprotkb:Q46702	intact:EBI-8793037	intact:EBI-8793044	psi-mi:q46705_ecolx(display_long)|uniprotkb:traF(gene name)|psi-mi:traF(display_short)	psi-mi:q46702_ecolx(display_long)|uniprotkb:traN(gene name)|psi-mi:traN(display_short)	psi-mi:"MI:0004"(affinity chromatography technology)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793035|imex:IM-21576-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0016021"(integral component of membrane)|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|interpro:IPR042217|rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46705|dip:DIP-59306N	rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46702|dip:DIP-59307N	-	crc64:3F94CFD32ED8316B	crc64:43ACC8EA9591FBAB	figure legend:f1b|comment:"Digestion of the FLCC with  elastase produced a stable truncated complex of around  880 kDa in molecular weight, termed the ‘CCelastase’  complex (Figure 1). The N-terminal sequencing of the two  main bands in SDS-PAGE gels confirms that the complex is  made of full-length TraO/VirB9 and the C-terminal domain of  TraF/VirB10 starting at N161SPGA (the integrity of the C  terminus of TraF/VirB10CTwas confirmed by western blotting  analysis of the Strep-tag present at the C terminus of the TraF/  VirB10 construct). Therefore, the IL of this complex is only made of TraO/VirB9NT (Figure 1). The expected molecular  weight of a 14-mer for this new truncated complex is  B830 kDa, which is very close to the measured molecular  weight indicated above"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:mZQ2W+QOEi3YR3r65VUIGsq1uUE562	rogid:PelN1kAP3SU0xdydQhO2QvHWrxU562	intact-crc:93CA955B2E623558|rigid:WL1/YE0EtX/VHq/qAi4l+6NvD8A	false	strep tag:c-c	-	-	-	psi-mi:"MI:0039"(edman degradation)	psi-mi:"MI:0039"(edman degradation)
uniprotkb:Q46705	uniprotkb:Q46704	intact:EBI-8793037	intact:EBI-8793050	psi-mi:q46705_ecolx(display_long)|uniprotkb:traF(gene name)|psi-mi:traF(display_short)	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:"MI:0004"(affinity chromatography technology)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793035|imex:IM-21576-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0016021"(integral component of membrane)|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|interpro:IPR042217|rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46705|dip:DIP-59306N	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N	-	crc64:3F94CFD32ED8316B	crc64:DCAE0E16EEB542FC	figure legend:f1b|comment:"Digestion of the FLCC with  elastase produced a stable truncated complex of around  880 kDa in molecular weight, termed the ‘CCelastase’  complex (Figure 1). The N-terminal sequencing of the two  main bands in SDS-PAGE gels confirms that the complex is  made of full-length TraO/VirB9 and the C-terminal domain of  TraF/VirB10 starting at N161SPGA (the integrity of the C  terminus of TraF/VirB10CTwas confirmed by western blotting  analysis of the Strep-tag present at the C terminus of the TraF/  VirB10 construct). Therefore, the IL of this complex is only made of TraO/VirB9NT (Figure 1). The expected molecular  weight of a 14-mer for this new truncated complex is  B830 kDa, which is very close to the measured molecular  weight indicated above"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:mZQ2W+QOEi3YR3r65VUIGsq1uUE562	rogid:d7V17+728ycIOavoJR8qtSeuemI562	intact-crc:93CA955B2E623558|rigid:WL1/YE0EtX/VHq/qAi4l+6NvD8A	false	strep tag:c-c	-	-	-	psi-mi:"MI:0039"(edman degradation)	psi-mi:"MI:0039"(edman degradation)
uniprotkb:Q46704	uniprotkb:Q46705	intact:EBI-8793050	intact:EBI-8793037	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:q46705_ecolx(display_long)|uniprotkb:traF(gene name)|psi-mi:traF(display_short)	psi-mi:"MI:0004"(affinity chromatography technology)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793072|imex:IM-21576-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N	go:"GO:0016021"(integral component of membrane)|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|interpro:IPR042217|rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46705|dip:DIP-59306N	-	crc64:DCAE0E16EEB542FC	crc64:3F94CFD32ED8316B	figure legend:f1b|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:d7V17+728ycIOavoJR8qtSeuemI562	rogid:mZQ2W+QOEi3YR3r65VUIGsq1uUE562	intact-crc:4835E5D0DCD4CEC1|rigid:eFnBFs9SasupXkLo5qHkOSGhIAU	false	-	binding-associated region:161-386|strep tag:c-c	-	-	psi-mi:"MI:0039"(edman degradation)	psi-mi:"MI:0039"(edman degradation)
uniprotkb:Q46702	uniprotkb:Q46704	intact:EBI-8793044	intact:EBI-8793050	psi-mi:q46702_ecolx(display_long)|uniprotkb:traN(gene name)|psi-mi:traN(display_short)	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:"MI:0071"(molecular sieving)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793108|imex:IM-21576-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46702|dip:DIP-59307N	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N	-	crc64:43ACC8EA9591FBAB	crc64:DCAE0E16EEB542FC	figure legend:f1c|comment:"Digestion of the FLCC with  elastase produced a stable truncated complex of around  880 kDa in molecular weight, termed the ‘CCelastase’  complex (Figure 1). The N-terminal sequencing of the two  main bands in SDS-PAGE gels confirms that the complex is  made of full-length TraO/VirB9 and the C-terminal domain of  TraF/VirB10 starting at N161SPGA (the integrity of the C  terminus of TraF/VirB10CTwas confirmed by western blotting  analysis of the Strep-tag present at the C terminus of the TraF/  VirB10 construct). Therefore, the IL of this complex is only made of TraO/VirB9NT (Figure 1). The expected molecular  weight of a 14-mer for this new truncated complex is  B830 kDa, which is very close to the measured molecular  weight indicated above"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:PelN1kAP3SU0xdydQhO2QvHWrxU562	rogid:d7V17+728ycIOavoJR8qtSeuemI562	intact-crc:BC16752B0A7B6AB3|rigid:WL1/YE0EtX/VHq/qAi4l+6NvD8A	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q46702	uniprotkb:Q46705	intact:EBI-8793044	intact:EBI-8793037	psi-mi:q46702_ecolx(display_long)|uniprotkb:traN(gene name)|psi-mi:traN(display_short)	psi-mi:q46705_ecolx(display_long)|uniprotkb:traF(gene name)|psi-mi:traF(display_short)	psi-mi:"MI:0071"(molecular sieving)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793108|imex:IM-21576-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46702|dip:DIP-59307N	go:"GO:0016021"(integral component of membrane)|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|interpro:IPR042217|rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46705|dip:DIP-59306N	-	crc64:43ACC8EA9591FBAB	crc64:3F94CFD32ED8316B	figure legend:f1c|comment:"Digestion of the FLCC with  elastase produced a stable truncated complex of around  880 kDa in molecular weight, termed the ‘CCelastase’  complex (Figure 1). The N-terminal sequencing of the two  main bands in SDS-PAGE gels confirms that the complex is  made of full-length TraO/VirB9 and the C-terminal domain of  TraF/VirB10 starting at N161SPGA (the integrity of the C  terminus of TraF/VirB10CTwas confirmed by western blotting  analysis of the Strep-tag present at the C terminus of the TraF/  VirB10 construct). Therefore, the IL of this complex is only made of TraO/VirB9NT (Figure 1). The expected molecular  weight of a 14-mer for this new truncated complex is  B830 kDa, which is very close to the measured molecular  weight indicated above"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:PelN1kAP3SU0xdydQhO2QvHWrxU562	rogid:mZQ2W+QOEi3YR3r65VUIGsq1uUE562	intact-crc:BC16752B0A7B6AB3|rigid:WL1/YE0EtX/VHq/qAi4l+6NvD8A	false	-	strep tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q46704	uniprotkb:Q46705	intact:EBI-8793050	intact:EBI-8793037	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:q46705_ecolx(display_long)|uniprotkb:traF(gene name)|psi-mi:traF(display_short)	psi-mi:"MI:0071"(molecular sieving)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793099|imex:IM-21576-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N	go:"GO:0016021"(integral component of membrane)|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|interpro:IPR042217|rcsb pdb:3JQO|rcsb pdb:3ZBI|mint:Q46705|dip:DIP-59306N	-	crc64:DCAE0E16EEB542FC	crc64:3F94CFD32ED8316B	figure legend:f1c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:d7V17+728ycIOavoJR8qtSeuemI562	rogid:mZQ2W+QOEi3YR3r65VUIGsq1uUE562	intact-crc:CBF9B2CA682C1AB6|rigid:eFnBFs9SasupXkLo5qHkOSGhIAU	false	-	binding-associated region:161-386|strep tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q46704	uniprotkb:Q46704	intact:EBI-8793050	intact:EBI-8793050	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:q46704_ecolx(display_long)|uniprotkb:traO(gene name)|psi-mi:traO(display_short)	psi-mi:"MI:0040"(electron microscopy)	Rivera-Calzada et al. (2013)	pubmed:23511972|imex:IM-21576	taxid:562(ecolx)|taxid:562(Escherichia coli)	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-8793234|imex:IM-21576-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N|emdb:EMD-2233|pdbe:2ypw	interpro:IPR010258(Conjugal transfer, TrbG/VirB9/CagX)|interpro:IPR033645|interpro:IPR038161|rcsb pdb:2YPW|rcsb pdb:3JQO|rcsb pdb:3ZBI|rcsb pdb:3ZBJ|mint:Q46704|dip:DIP-59308N|emdb:EMD-2233|pdbe:2ypw	emdb:EMD-2232|pdbe:3zbi|pdbe:3zbj	crc64:DCAE0E16EEB542FC	crc64:DCAE0E16EEB542FC	figure legend:f2|comment:"Digestion of the FLCC with  elastase produced a stable truncated complex of around  880 kDa in molecular weight, termed the ‘CCelastase’  complex (Figure 1). The N-terminal sequencing of the two  main bands in SDS-PAGE gels confirms that the complex is  made of full-length TraO/VirB9 and the C-terminal domain of  TraF/VirB10 starting at N161SPGA (the integrity of the C  terminus of TraF/VirB10CTwas confirmed by western blotting  analysis of the Strep-tag present at the C terminus of the TraF/  VirB10 construct). Therefore, the IL of this complex is only made of TraO/VirB9NT (Figure 1). The expected molecular  weight of a 14-mer for this new truncated complex is  B830 kDa, which is very close to the measured molecular  weight indicated above"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/10/11	2014/10/16	rogid:d7V17+728ycIOavoJR8qtSeuemI562	rogid:d7V17+728ycIOavoJR8qtSeuemI562	intact-crc:7304554B0B6C6228|rigid:Y4aTk6j2uXuWtu2ruQOIRbxwpq4	false	-	-	14	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)