#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8870868|imex:IM-21738-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 4a, b|comment:"The thiolation of tRNA(Glu) is enhanced by the addition of TusA (P0A890), TusA and TusBCD complex (P45530, P45531, P45532), TusA and TusE (P0AB18) and - rather significantly - enhanced by the addition of TusA, TusBCD and TusE."|comment:IscS and MnmA are the minimum essential factors for 2-thiolation of mnm5s2U;  addition of TusA alone enhances the interaction, and addition of the  TusBCD complex and/or TusE  stimulates 2-thiolation activity further.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:AA7B426A622AAB41	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P25745	intact:EBI-2014180	intact:EBI-1122413|uniprotkb:P75964|ensemblbacteria:AAC74217|ensemblbacteria:BAA35955	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:mnma_ecoli(display_long)|uniprotkb:mnmA(gene name)|psi-mi:mnmA(display_short)|uniprotkb:asuE(gene name synonym)|uniprotkb:trmU(gene name synonym)|uniprotkb:b1133(locus name)|uniprotkb:ycfB(gene name synonym)|uniprotkb:JW1119(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8870868|imex:IM-21738-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_415651.4|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR023382|rcsb pdb:2DER|rcsb pdb:2DET|rcsb pdb:2DEU|interpro:IPR004506("tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase")|ensemblbacteria:AAC74217(transcript)|ensemblbacteria:b1133(gene)|ensemblbacteria:BAA35955(gene)|ensemblbacteria:BAA35955(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|refseq:WP_001297484.1|dip:DIP-11035N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 4a, b|comment:"The thiolation of tRNA(Glu) is enhanced by the addition of TusA (P0A890), TusA and TusBCD complex (P45530, P45531, P45532), TusA and TusE (P0AB18) and - rather significantly - enhanced by the addition of TusA, TusBCD and TusE."|comment:IscS and MnmA are the minimum essential factors for 2-thiolation of mnm5s2U;  addition of TusA alone enhances the interaction, and addition of the  TusBCD complex and/or TusE  stimulates 2-thiolation activity further.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:k0pkYEInGq3SaBk5fIrhYEqTbHY83333	intact-crc:AA7B426A622AAB41	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	intact:EBI-6555944	intact:EBI-2014180	chebi:"CHEBI:29175"	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:trnaglu(display_short)|psi-mi:EBI-6555944(display_long)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8870868|imex:IM-21738-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0325"(transfer rna)	-	-	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 4a, b|comment:"The thiolation of tRNA(Glu) is enhanced by the addition of TusA (P0A890), TusA and TusBCD complex (P45530, P45531, P45532), TusA and TusE (P0AB18) and - rather significantly - enhanced by the addition of TusA, TusBCD and TusE."|comment:IscS and MnmA are the minimum essential factors for 2-thiolation of mnm5s2U;  addition of TusA alone enhances the interaction, and addition of the  TusBCD complex and/or TusE  stimulates 2-thiolation activity further.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	-	intact-crc:AA7B426A622AAB41	false	35s radiolabel:?-?	mutation:8-8|mutation disrupting interaction:34-34	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871732|imex:IM-21738-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	-	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 6d  lane 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:91E44DA89B6D5573	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871732|imex:IM-21738-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	-	-	-	figure legend:Fig. 6d  lane 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:91E44DA89B6D5573	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P45532	intact:EBI-2014180	intact:EBI-8871402|uniprotkb:Q2M710|ensemblbacteria:AAC76370|ensemblbacteria:BAE77946	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusd_ecoli(display_long)|uniprotkb:tusD(gene name)|psi-mi:tusD(display_short)|uniprotkb:yheN(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein D(gene name synonym)|uniprotkb:b3345(locus name)|uniprotkb:JW3307(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871732|imex:IM-21738-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_001209680.1|refseq:NP_417804.1|dip:DIP-12310N|ensemblbacteria:AAC76370(transcript)|ensemblbacteria:b3345(gene)|ensemblbacteria:BAE77946(gene)|ensemblbacteria:BAE77946(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|go:"GO:0097163"(sulfur carrier activity)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR003787(Sulphur relay, DsrE-like protein)|interpro:IPR017463(Sulphur relay, TusD/DsrE)|rcsb pdb:2D1P|interpro:IPR027396	-	-	crc64:87B2754849A0FEFF	figure legend:Fig. 6d  lane 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:ydplRfzxhniQODh+1rbeag3wQn083333	intact-crc:91E44DA89B6D5573	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9082234|imex:IM-21738-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 6d  lane 6|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:F50A5A65B81770B4	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9082234|imex:IM-21738-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6d  lane 6|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:F50A5A65B81770B4	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0AB18	intact:EBI-2014180	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9082234|imex:IM-21738-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6d  lane 6|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:F50A5A65B81770B4	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871723|imex:IM-21738-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 6d  lane 7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:CF924B6BDF480D18	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871723|imex:IM-21738-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6d  lane 7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:CF924B6BDF480D18	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P45532	intact:EBI-2014180	intact:EBI-8871402|uniprotkb:Q2M710|ensemblbacteria:AAC76370|ensemblbacteria:BAE77946	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusd_ecoli(display_long)|uniprotkb:tusD(gene name)|psi-mi:tusD(display_short)|uniprotkb:yheN(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein D(gene name synonym)|uniprotkb:b3345(locus name)|uniprotkb:JW3307(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871723|imex:IM-21738-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_001209680.1|refseq:NP_417804.1|dip:DIP-12310N|ensemblbacteria:AAC76370(transcript)|ensemblbacteria:b3345(gene)|ensemblbacteria:BAE77946(gene)|ensemblbacteria:BAE77946(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|go:"GO:0097163"(sulfur carrier activity)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR003787(Sulphur relay, DsrE-like protein)|interpro:IPR017463(Sulphur relay, TusD/DsrE)|rcsb pdb:2D1P|interpro:IPR027396	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:87B2754849A0FEFF	figure legend:Fig. 6d  lane 7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:ydplRfzxhniQODh+1rbeag3wQn083333	intact-crc:CF924B6BDF480D18	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0AB18	intact:EBI-2014180	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871723|imex:IM-21738-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6d  lane 7|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:CF924B6BDF480D18	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871740|imex:IM-21738-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 6d lane 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:A3BCB5A72616B71B	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871740|imex:IM-21738-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6d lane 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:A3BCB5A72616B71B	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871747|imex:IM-21738-13	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 6d lane 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:D08D08A47881ED54	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	chebi:"CHEBI:18405"	intact:EBI-2014180	intact:EBI-8851905|chebi:"CHEBI:8668"|chebi:"CHEBI:14977"|chebi:"CHEBI:26424"|chebi:"CHEBI:358848"|chebi:"CHEBI:45145"|chebi:"CHEBI:234571"	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:pyridoxal 5'-phosphate(display_short)|psi-mi:"CHEBI:18405"(display_long)|intact:"3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde"(synonym)|intact:"3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate"(synonym)|intact:codecarboxylase(synonym)|intact:"Phosphoric acid mono-(4-formyl-5-hydroxy-6-methyl-pyridin-3-ylmethyl) ester"(synonym)|intact:pyridoxal  5-monophosphoric acid ester(synonym)|intact:"pyridoxal 5'-(dihydrogen phosphate)"(synonym)|intact:Pyridoxal 5'-phosphate(synonym)|intact:Pyridoxal 5-phosphate(synonym)|intact:Pyridoxal phosphate(synonym)|intact:PYRIDOXAL-5'-PHOSPHATE(synonym)|intact:"(4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate"(iupac name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0682"(cofactor)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	go:"GO:0016783"(sulfurtransferase activity)	-	inchi key:NGVDGCNFYWLIFO-UHFFFAOYSA-N	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	-	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	-	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	intact:EBI-6555944	intact:EBI-2014180	chebi:"CHEBI:29175"	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:trnaglu(display_short)|psi-mi:EBI-6555944(display_long)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0325"(transfer rna)	-	-	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	-	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	mutation:8-8|mutation disrupting interaction:34-34	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P45532	intact:EBI-2014180	intact:EBI-8871402|uniprotkb:Q2M710|ensemblbacteria:AAC76370|ensemblbacteria:BAE77946	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusd_ecoli(display_long)|uniprotkb:tusD(gene name)|psi-mi:tusD(display_short)|uniprotkb:yheN(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein D(gene name synonym)|uniprotkb:b3345(locus name)|uniprotkb:JW3307(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_001209680.1|refseq:NP_417804.1|dip:DIP-12310N|ensemblbacteria:AAC76370(transcript)|ensemblbacteria:b3345(gene)|ensemblbacteria:BAE77946(gene)|ensemblbacteria:BAE77946(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|go:"GO:0097163"(sulfur carrier activity)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR003787(Sulphur relay, DsrE-like protein)|interpro:IPR017463(Sulphur relay, TusD/DsrE)|rcsb pdb:2D1P|interpro:IPR027396	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:87B2754849A0FEFF	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:ydplRfzxhniQODh+1rbeag3wQn083333	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0AB18	intact:EBI-2014180	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871372|imex:IM-21738-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 4c|comment:"In addition to the 2-thiouridine of the tRNAGlu(U8C), IscS, TusA, TusD, and TusE were clearly labeled by [35S].  These labeled protein bands disappeared when treated with reducing agent (DTT)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:AC97F712F03CA529	false	35s radiolabel:?-?	his tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A6B7	intact:EBI-2014180	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871472|imex:IM-21738-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	go:"GO:0016783"(sulfurtransferase activity)	-	crc64:1E4F2E6F9CD266B0	figure legend:Fig. 5b, d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	intact-crc:51A53DE9D5191785	false	35s radiolabel:?-?	his tag:n-n	-	2	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	chebi:"CHEBI:18405"	intact:EBI-2014180	intact:EBI-8851905|chebi:"CHEBI:8668"|chebi:"CHEBI:14977"|chebi:"CHEBI:26424"|chebi:"CHEBI:358848"|chebi:"CHEBI:45145"|chebi:"CHEBI:234571"	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:pyridoxal 5'-phosphate(display_short)|psi-mi:"CHEBI:18405"(display_long)|intact:"3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde"(synonym)|intact:"3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate"(synonym)|intact:codecarboxylase(synonym)|intact:"Phosphoric acid mono-(4-formyl-5-hydroxy-6-methyl-pyridin-3-ylmethyl) ester"(synonym)|intact:pyridoxal  5-monophosphoric acid ester(synonym)|intact:"pyridoxal 5'-(dihydrogen phosphate)"(synonym)|intact:Pyridoxal 5'-phosphate(synonym)|intact:Pyridoxal 5-phosphate(synonym)|intact:Pyridoxal phosphate(synonym)|intact:PYRIDOXAL-5'-PHOSPHATE(synonym)|intact:"(4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate"(iupac name)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871472|imex:IM-21738-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0682"(cofactor)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	go:"GO:0016783"(sulfurtransferase activity)	-	inchi key:NGVDGCNFYWLIFO-UHFFFAOYSA-N	figure legend:Fig. 5b, d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	-	intact-crc:51A53DE9D5191785	false	35s radiolabel:?-?	-	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15356"	uniprotkb:P0A890	intact:EBI-2014180	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:cysteine(display_short)|psi-mi:"CHEBI:15356"(display_long)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:1325"(sulfurtransferase assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:1327"(sulfurtransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871472|imex:IM-21738-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 5b, d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2018/12/07	-	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:51A53DE9D5191785	false	35s radiolabel:?-?	his tag:n-n|mutation disrupting interaction:19-19	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
uniprotkb:P25745	uniprotkb:P0AB18	intact:EBI-1122413|uniprotkb:P75964|ensemblbacteria:AAC74217|ensemblbacteria:BAA35955	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:mnma_ecoli(display_long)|uniprotkb:mnmA(gene name)|psi-mi:mnmA(display_short)|uniprotkb:asuE(gene name synonym)|uniprotkb:trmU(gene name synonym)|uniprotkb:b1133(locus name)|uniprotkb:ycfB(gene name synonym)|uniprotkb:JW1119(locus name)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:0071"(molecular sieving)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871637|imex:IM-21738-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_415651.4|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR023382|rcsb pdb:2DER|rcsb pdb:2DET|rcsb pdb:2DEU|interpro:IPR004506("tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase")|ensemblbacteria:AAC74217(transcript)|ensemblbacteria:b1133(gene)|ensemblbacteria:BAA35955(gene)|ensemblbacteria:BAA35955(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|refseq:WP_001297484.1|dip:DIP-11035N	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	-	-	-	figure legend:Fig. 6c|caution:Figure legends and text references for Figures 6b and 6c have been inverted!|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:k0pkYEInGq3SaBk5fIrhYEqTbHY83333	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:FD2D54DD50987FFF|rigid:xDjtPcqa+rO6rDpPLSGT3OmvlfQ	false	his tag:n-n	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P45530	uniprotkb:P45531	intact:EBI-8871385|uniprotkb:Q2M708|ensemblbacteria:AAC76368|ensemblbacteria:BAE77948	intact:EBI-546671|uniprotkb:Q2M709|ensemblbacteria:AAC76369|ensemblbacteria:BAE77947	psi-mi:tusb_ecoli(display_long)|uniprotkb:tusB(gene name)|psi-mi:tusB(display_short)|uniprotkb:yheL(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein B(gene name synonym)|uniprotkb:b3343(locus name)|uniprotkb:JW3305(locus name)	psi-mi:tusc_ecoli(display_long)|uniprotkb:tusC(gene name)|psi-mi:tusC(display_short)|uniprotkb:b3344(locus name)|uniprotkb:tRNA 2-thiouridine synthesizing protein C(gene name synonym)|uniprotkb:yheM(gene name synonym)|uniprotkb:JW3306(locus name)	psi-mi:"MI:0071"(molecular sieving)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871626|imex:IM-21738-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000903373.1|refseq:NP_417802.1|dip:DIP-12308N|ensemblbacteria:AAC76368(transcript)|ensemblbacteria:b3343(gene)|ensemblbacteria:BAE77948(gene)|ensemblbacteria:BAE77948(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR007215(Sulphur relay, TusB/DsrH)|interpro:IPR023526|interpro:IPR027396|rcsb pdb:2D1P	refseq:NP_417803.1|ensemblbacteria:AAC76369(transcript)|ensemblbacteria:b3344(gene)|ensemblbacteria:BAE77947(gene)|ensemblbacteria:BAE77947(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR003787(Sulphur relay, DsrE-like protein)|interpro:IPR017462(Sulphur relay, TusC/DsrF)|interpro:IPR027396|interpro:IPR037450|rcsb pdb:2D1P|refseq:WP_000820714.1|dip:DIP-12309N	-	crc64:0EEF7560CCB4D608	crc64:2AF593ADC435CA0D	figure legend:Fig. 6b|caution:Figure legends and text references for Figures 6b and 6c have been inverted!|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:s4CRFx4DZm76S5mhbV/LbcOg/rQ83333	rogid:nJuWaXWt7rlYs55LokGBzBdGl/083333	intact-crc:291DF7A18C3E8ACE|rigid:NhW2HLx2+8xUdlB9r5CkAUrSsIo	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P45530	uniprotkb:P45532	intact:EBI-8871385|uniprotkb:Q2M708|ensemblbacteria:AAC76368|ensemblbacteria:BAE77948	intact:EBI-8871402|uniprotkb:Q2M710|ensemblbacteria:AAC76370|ensemblbacteria:BAE77946	psi-mi:tusb_ecoli(display_long)|uniprotkb:tusB(gene name)|psi-mi:tusB(display_short)|uniprotkb:yheL(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein B(gene name synonym)|uniprotkb:b3343(locus name)|uniprotkb:JW3305(locus name)	psi-mi:tusd_ecoli(display_long)|uniprotkb:tusD(gene name)|psi-mi:tusD(display_short)|uniprotkb:yheN(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein D(gene name synonym)|uniprotkb:b3345(locus name)|uniprotkb:JW3307(locus name)	psi-mi:"MI:0071"(molecular sieving)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871626|imex:IM-21738-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000903373.1|refseq:NP_417802.1|dip:DIP-12308N|ensemblbacteria:AAC76368(transcript)|ensemblbacteria:b3343(gene)|ensemblbacteria:BAE77948(gene)|ensemblbacteria:BAE77948(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR007215(Sulphur relay, TusB/DsrH)|interpro:IPR023526|interpro:IPR027396|rcsb pdb:2D1P	refseq:WP_001209680.1|refseq:NP_417804.1|dip:DIP-12310N|ensemblbacteria:AAC76370(transcript)|ensemblbacteria:b3345(gene)|ensemblbacteria:BAE77946(gene)|ensemblbacteria:BAE77946(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|go:"GO:0097163"(sulfur carrier activity)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR003787(Sulphur relay, DsrE-like protein)|interpro:IPR017463(Sulphur relay, TusD/DsrE)|rcsb pdb:2D1P|interpro:IPR027396	-	crc64:0EEF7560CCB4D608	crc64:87B2754849A0FEFF	figure legend:Fig. 6b|caution:Figure legends and text references for Figures 6b and 6c have been inverted!|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:s4CRFx4DZm76S5mhbV/LbcOg/rQ83333	rogid:ydplRfzxhniQODh+1rbeag3wQn083333	intact-crc:291DF7A18C3E8ACE|rigid:NhW2HLx2+8xUdlB9r5CkAUrSsIo	false	-	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P45530	uniprotkb:P0AB18	intact:EBI-8871385|uniprotkb:Q2M708|ensemblbacteria:AAC76368|ensemblbacteria:BAE77948	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:tusb_ecoli(display_long)|uniprotkb:tusB(gene name)|psi-mi:tusB(display_short)|uniprotkb:yheL(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein B(gene name synonym)|uniprotkb:b3343(locus name)|uniprotkb:JW3305(locus name)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:0071"(molecular sieving)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871626|imex:IM-21738-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000903373.1|refseq:NP_417802.1|dip:DIP-12308N|ensemblbacteria:AAC76368(transcript)|ensemblbacteria:b3343(gene)|ensemblbacteria:BAE77948(gene)|ensemblbacteria:BAE77948(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:1990228"(sulfurtransferase complex)|interpro:IPR007215(Sulphur relay, TusB/DsrH)|interpro:IPR023526|interpro:IPR027396|rcsb pdb:2D1P	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	-	crc64:0EEF7560CCB4D608	-	figure legend:Fig. 6b|caution:Figure legends and text references for Figures 6b and 6c have been inverted!|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:s4CRFx4DZm76S5mhbV/LbcOg/rQ83333	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:291DF7A18C3E8ACE|rigid:NhW2HLx2+8xUdlB9r5CkAUrSsIo	false	-	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0A6B7	uniprotkb:P0A890	intact:EBI-550055|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAT48142|ensemblbacteria:BAA16424	intact:EBI-561780|uniprotkb:Q2M7D3|ensemblbacteria:AAC76495|ensemblbacteria:BAE77823|uniprotkb:P37618	psi-mi:iscs_ecoli(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:b2530(locus name)|uniprotkb:JW2514(locus name)|uniprotkb:yfhO(gene name synonym)|uniprotkb:yzzO(gene name synonym)|uniprotkb:ThiI transpersulfidase(gene name synonym)|uniprotkb:NifS protein homolog(gene name synonym)|uniprotkb:TusA transpersulfidase(gene name synonym)|uniprotkb:nuvC(gene name synonym)	psi-mi:tusa_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:b3470(locus name)|uniprotkb:sirA(gene name synonym)|uniprotkb:yhhP(gene name synonym)|uniprotkb:JW3435(locus name)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871460|imex:IM-21738-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:YP_026169.1|refseq:WP_001295373.1|dip:DIP-29109N|ensemblbacteria:AAT48142(transcript)|ensemblbacteria:b2530(gene)|ensemblbacteria:BAA16424(gene)|ensemblbacteria:BAA16424(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009000"(selenocysteine lyase activity)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0018131"(oxazole or thiazole biosynthetic process)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0031119"(tRNA pseudouridine synthesis)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:1P3W	ensemblbacteria:AAC76495(transcript)|ensemblbacteria:b3470(gene)|ensemblbacteria:BAE77823(gene)|ensemblbacteria:BAE77823(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005829"(cytosol)|go:"GO:0006777"(Mo-molybdopterin cofactor biosynthetic process)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:1DCJ|refseq:NP_417927.1|refseq:WP_000130621.1|dip:DIP-48202N	-	crc64:1E4F2E6F9CD266B0	-	figure legend:Fig. 5a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83333	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83333	intact-crc:6E3360E59B70BC62|rigid:GYb9td7y3ECJN8PLIgiLrmB9bxA	false	his tag:n-n	his tag:n-n	2	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P25745	intact:EBI-6555944	intact:EBI-1122413|uniprotkb:P75964|ensemblbacteria:AAC74217|ensemblbacteria:BAA35955	chebi:"CHEBI:29175"	psi-mi:mnma_ecoli(display_long)|uniprotkb:mnmA(gene name)|psi-mi:mnmA(display_short)|uniprotkb:asuE(gene name synonym)|uniprotkb:trmU(gene name synonym)|uniprotkb:b1133(locus name)|uniprotkb:ycfB(gene name synonym)|uniprotkb:JW1119(locus name)	psi-mi:trnaglu(display_short)|psi-mi:EBI-6555944(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871568|imex:IM-21738-12	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0325"(transfer rna)	refseq:NP_415651.4|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR023382|rcsb pdb:2DER|rcsb pdb:2DET|rcsb pdb:2DEU|interpro:IPR004506("tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase")|ensemblbacteria:AAC74217(transcript)|ensemblbacteria:b1133(gene)|ensemblbacteria:BAA35955(gene)|ensemblbacteria:BAA35955(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|refseq:WP_001297484.1|dip:DIP-11035N	-	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	rogid:k0pkYEInGq3SaBk5fIrhYEqTbHY83333	-	intact-crc:A2ACF7F848AAA2EE	false	his tag:n-n	mutation:8-8	-	-	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)
intact:EBI-6555944	uniprotkb:P25745	chebi:"CHEBI:29175"	intact:EBI-1122413|uniprotkb:P75964|ensemblbacteria:AAC74217|ensemblbacteria:BAA35955	psi-mi:trnaglu(display_short)|psi-mi:EBI-6555944(display_long)	psi-mi:mnma_ecoli(display_long)|uniprotkb:mnmA(gene name)|psi-mi:mnmA(display_short)|uniprotkb:asuE(gene name synonym)|uniprotkb:trmU(gene name synonym)|uniprotkb:b1133(locus name)|uniprotkb:ycfB(gene name synonym)|uniprotkb:JW1119(locus name)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871587|imex:IM-21738-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0325"(transfer rna)	psi-mi:"MI:0326"(protein)	-	refseq:NP_415651.4|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR023382|rcsb pdb:2DER|rcsb pdb:2DET|rcsb pdb:2DEU|interpro:IPR004506("tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase")|ensemblbacteria:AAC74217(transcript)|ensemblbacteria:b1133(gene)|ensemblbacteria:BAA35955(gene)|ensemblbacteria:BAA35955(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005524"(ATP binding)|go:"GO:0005829"(cytosol)|go:"GO:0016783"(sulfurtransferase activity)|refseq:WP_001297484.1|dip:DIP-11035N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	-	rogid:k0pkYEInGq3SaBk5fIrhYEqTbHY83333	intact-crc:F72EF36E6B8E986D	false	mutation:8-8	his tag:n-n	-	-	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)
intact:EBI-6555944	uniprotkb:P0AB18	chebi:"CHEBI:29175"	intact:EBI-1121167|uniprotkb:P45572|uniprotkb:P75878|ensemblbacteria:AAC74055|ensemblbacteria:BAA35734	psi-mi:trnaglu(display_short)|psi-mi:EBI-6555944(display_long)	psi-mi:tuse_ecoli(display_long)|uniprotkb:tRNA 2-thiouridine synthesizing protein E(gene name synonym)|uniprotkb:tusE(gene name)|psi-mi:tusE(display_short)|uniprotkb:yccK(gene name synonym)|uniprotkb:b0969(locus name)|uniprotkb:JW0952(locus name)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Ikeuchi et al. (2006)	imex:IM-21738|pubmed:16387657	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-8871587|imex:IM-21738-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0325"(transfer rna)	psi-mi:"MI:0326"(protein)	-	refseq:WP_000904442.1|ensemblbacteria:AAC74055(transcript)|ensemblbacteria:b0969(gene)|ensemblbacteria:BAA35734(gene)|ensemblbacteria:BAA35734(transcript)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0005737"(cytoplasm)|go:"GO:0016740"(transferase activity)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR007453(DsrC-like protein)|interpro:IPR025526|interpro:IPR042072|interpro:IPR043163|refseq:NP_415489.4|dip:DIP-48154N	go:"GO:0016783"(sulfurtransferase activity)	-	-	figure legend:Fig. 6a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2013/11/21	2014/10/16	-	rogid:FovnlRW6svajCxVK1IPLup8PLzU83333	intact-crc:F72EF36E6B8E986D	false	mutation:8-8	his tag:n-n	-	-	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)	psi-mi:"MI:0819"(molecular weight estimation by bromide staining)