#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P30140 uniprotkb:P30139 intact:EBI-1125553|uniprotkb:Q2M8S6|ensemblbacteria:AAC76964|ensemblbacteria:BAE77330 intact:EBI-547059|uniprotkb:P76779|uniprotkb:Q2M8S7|ensemblbacteria:AAC76965|ensemblbacteria:BAE77329 psi-mi:thih_ecoli(display_long)|uniprotkb:thiH(gene name)|psi-mi:thiH(display_short)|uniprotkb:b3990(locus name)|uniprotkb:JW3953(locus name)|uniprotkb:Tyrosine lyase(gene name synonym)|uniprotkb:Dehydroglycine synthase(gene name synonym) psi-mi:thig_ecoli(display_long)|uniprotkb:thiG(gene name)|psi-mi:thiG(display_short)|uniprotkb:b3991(locus name)|uniprotkb:JW5549(locus name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Leonardi et al. (2003) pubmed:12650933|imex:IM-21718 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-8851178|imex:IM-21718-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_418417.1|refseq:WP_000847447.1|ensemblbacteria:AAC76964(transcript)|ensemblbacteria:b3990(gene)|ensemblbacteria:BAE77330(gene)|ensemblbacteria:BAE77330(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0009228"(thiamine biosynthetic process)|go:"GO:0009229"(thiamine diphosphate biosynthetic process)|go:"GO:0036355"(2-iminoacetate synthase activity)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR007197(Radical SAM)|interpro:IPR010722(Biotin and thiamin synthesis associated)|interpro:IPR012726(Thiazole biosynthesis ThiH)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR034428|dip:DIP-6869N refseq:NP_418418.2|refseq:WP_000944104.1|ensemblbacteria:AAC76965(transcript)|ensemblbacteria:b3991(gene)|ensemblbacteria:BAE77329(gene)|ensemblbacteria:BAE77329(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009228"(thiamine biosynthetic process)|go:"GO:0009229"(thiamine diphosphate biosynthetic process)|go:"GO:1990107"(thiazole synthase activity)|interpro:IPR008867(Thiazole biosynthesis)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR033983|dip:DIP-6868N - - - figure legend:Fig. 2a|comment:The 26kD band was much stronger for the plasmids expressing ThiFSGH.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:469008(ecobd)|taxid:469008("Escherichia coli (strain B / BL21-DE3)") - 2013/11/14 2014/10/16 rogid:bDf6JPJJVqqEfTyvin4Es9wgAZs83333 rogid:T23fRQ3hxMlUa7Av2I7REQapmUg83333 intact-crc:7FB77E01DB7E5F34|rigid:+UOLPvHZCpwLOB4jNICTL8RIoNg false his tag:c-c - - - psi-mi:"MI:0093"(protein sequence identification) psi-mi:"MI:0093"(protein sequence identification) uniprotkb:P30139 uniprotkb:P30140 intact:EBI-547059|uniprotkb:P76779|uniprotkb:Q2M8S7|ensemblbacteria:AAC76965|ensemblbacteria:BAE77329 intact:EBI-1125553|uniprotkb:Q2M8S6|ensemblbacteria:AAC76964|ensemblbacteria:BAE77330 psi-mi:thig_ecoli(display_long)|uniprotkb:thiG(gene name)|psi-mi:thiG(display_short)|uniprotkb:b3991(locus name)|uniprotkb:JW5549(locus name) psi-mi:thih_ecoli(display_long)|uniprotkb:thiH(gene name)|psi-mi:thiH(display_short)|uniprotkb:b3990(locus name)|uniprotkb:JW3953(locus name)|uniprotkb:Tyrosine lyase(gene name synonym)|uniprotkb:Dehydroglycine synthase(gene name synonym) psi-mi:"MI:0071"(molecular sieving) Leonardi et al. (2003) pubmed:12650933|imex:IM-21718 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-8851209|imex:IM-21718-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_418418.2|refseq:WP_000944104.1|ensemblbacteria:AAC76965(transcript)|ensemblbacteria:b3991(gene)|ensemblbacteria:BAE77329(gene)|ensemblbacteria:BAE77329(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0009228"(thiamine biosynthetic process)|go:"GO:0009229"(thiamine diphosphate biosynthetic process)|go:"GO:1990107"(thiazole synthase activity)|interpro:IPR008867(Thiazole biosynthesis)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR033983|dip:DIP-6868N refseq:NP_418417.1|refseq:WP_000847447.1|ensemblbacteria:AAC76964(transcript)|ensemblbacteria:b3990(gene)|ensemblbacteria:BAE77330(gene)|ensemblbacteria:BAE77330(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006974"(cellular response to DNA damage stimulus)|go:"GO:0009228"(thiamine biosynthetic process)|go:"GO:0009229"(thiamine diphosphate biosynthetic process)|go:"GO:0036355"(2-iminoacetate synthase activity)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR007197(Radical SAM)|interpro:IPR010722(Biotin and thiamin synthesis associated)|interpro:IPR012726(Thiazole biosynthesis ThiH)|interpro:IPR013785(Aldolase-type TIM barrel)|interpro:IPR034428|dip:DIP-6869N - - - figure legend:Fig. 2b|comment:The 26kD band was much stronger for the plasmids expressing ThiFSGH.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/11/14 2014/10/16 rogid:T23fRQ3hxMlUa7Av2I7REQapmUg83333 rogid:bDf6JPJJVqqEfTyvin4Es9wgAZs83333 intact-crc:6BD9D20BFE8BFD23|rigid:+UOLPvHZCpwLOB4jNICTL8RIoNg false - his tag:c-c - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining)