#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P0ACF8 uniprotkb:P0ACF8 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:"MI:0077"(nuclear magnetic resonance) Bloch et al. (2003) pubmed:12592399|imex:IM-20845 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-6692727|wwpdb:1ni8|imex:IM-20845-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N - - - figure legend:Fig. 2|comment:"The experiment was carried out with 2 constructs: a) a recombinant protein with a n-terminal his-tag, expressed in ecoli and b) a synthetic peptide."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/05/29 2014/10/16 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 intact-crc:A950F048A208C92E|rigid:7lX38iKtnbZwS3FwjAmiog4u8sI false 15n label:?-?|13c label:?-?|sufficient binding region:2-47 15n label:?-?|13c label:?-?|sufficient binding region:2-47 2 0 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0ACF8 uniprotkb:P0ACF8 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:"MI:0053"(fluorescence polarization spectroscopy) Bloch et al. (2003) pubmed:12592399|imex:IM-20845 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-6693427|imex:IM-20845-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N - - - figure legend:Fig. 1|comment:The dimer occurs predominantly at low concentrations.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/05/29 2014/10/16 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 intact-crc:6DCE9159BF4FD583|rigid:7lX38iKtnbZwS3FwjAmiog4u8sI false alexa 488 label:n-n|sufficient binding region:2-47 alexa 488 label:n-n|sufficient binding region:2-47 2 0 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0ACF8 uniprotkb:P0ACF8 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:"MI:0053"(fluorescence polarization spectroscopy) Bloch et al. (2003) pubmed:12592399|imex:IM-20845 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-6693455|imex:IM-20845-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N - - - figure legend:Fig. 1|comment:The oligomers form at higher concentrations.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/05/29 2014/10/16 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 intact-crc:A0FD038AF2456E35|rigid:ic4NdmwppSEdvN4dzKHXSRmNVDA false sufficient binding region:2-47|alexa 488 label:n-n sufficient binding region:2-47|alexa 488 label:n-n - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0ACF8 uniprotkb:P0ACF8 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:"MI:0030"(cross-linking study) Bloch et al. (2003) pubmed:12592399|imex:IM-20845 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-6693495|imex:IM-20845-4 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N - - - figure legend:Fig. 1|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/05/29 2014/10/16 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 intact-crc:71E26CB4CD78496D|rigid:7lX38iKtnbZwS3FwjAmiog4u8sI false sufficient binding region:2-47 sufficient binding region:2-47 2 0 psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) psi-mi:"MI:0818"(molecular weight estimation by coomasie staining) uniprotkb:P0ACF8 intact:EBI-6860602 intact:EBI-544934|uniprotkb:P08936|ensemblbacteria:AAC74319|ensemblbacteria:BAA36117|uniprotkb:Q47267 pubmed:11886561 psi-mi:hns_ecoli(display_long)|uniprotkb:hns(gene name)|psi-mi:hns(display_short)|uniprotkb:bglY(gene name synonym)|uniprotkb:cur(gene name synonym)|uniprotkb:drdX(gene name synonym)|uniprotkb:hnsA(gene name synonym)|uniprotkb:msyA(gene name synonym)|uniprotkb:osmZ(gene name synonym)|uniprotkb:pilG(gene name synonym)|uniprotkb:topS(gene name synonym)|uniprotkb:b1237(locus name)|uniprotkb:Histone-like protein HLP-II(gene name synonym)|uniprotkb:Protein H1(gene name synonym)|uniprotkb:Protein B1(gene name synonym)|uniprotkb:JW1225(locus name)|uniprotkb:Heat-stable nucleoid-structuring protein(gene name synonym) psi-mi:curved dna(display_short)|psi-mi:EBI-6860602(display_long) psi-mi:"MI:0413"(electrophoretic mobility shift assay) Bloch et al. (2003) pubmed:12592399|imex:IM-20845 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-6693507|imex:IM-20845-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0681"(double stranded deoxyribonucleic acid) refseq:NP_415753.1|ensemblbacteria:AAC74319(transcript)|ensemblbacteria:b1237(gene)|ensemblbacteria:BAA36117(gene)|ensemblbacteria:BAA36117(transcript)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0001217"(DNA-binding transcription repressor activity)|go:"GO:0003680"(minor groove of adenine-thymine-rich DNA binding)|go:"GO:0003681"(bent DNA binding)|go:"GO:0003723"(RNA binding)|go:"GO:0005829"(cytosol)|go:"GO:0006417"(regulation of translation)|go:"GO:0009295"(nucleoid)|go:"GO:0016020"(membrane)|go:"GO:0016458"(obsolete gene silencing)|go:"GO:0032993"(protein-DNA complex)|go:"GO:0042802"(identical protein binding)|go:"GO:0045892"(negative regulation of transcription, DNA-templated)|go:"GO:0046983"(protein dimerization activity)|go:"GO:1900232"(negative regulation of single-species biofilm formation on inanimate substrate)|interpro:IPR001801(Histone-like nucleoid-structuring protein H-NS)|interpro:IPR027444|interpro:IPR027454|interpro:IPR037150|rcsb pdb:1HNR|rcsb pdb:1HNS|rcsb pdb:1LR1|rcsb pdb:1NI8|rcsb pdb:2MW2|refseq:WP_001287378.1|dip:DIP-35853N - - - - figure legend:Fig. 4b|comment:"DNA binding is concentration dependent: A molar ratio of at least 4:1 protein:DNA is required for binding."|comment:The double mutant recognizes DNA with a slightly weaker affinity than the wild type molecule, and it has lost its ability to preferentially recognize intrinsically curved DNA sequences.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2013/05/29 2014/10/16 rogid:z3zhSY/wN/7D3J5NmXhXr01GTtg83333 - intact-crc:C9BE5217AFCF74F7 false his tag:n-n|mutation decreasing interaction:12-12,15-15 - - - psi-mi:"MI:0819"(molecular weight estimation by bromide staining) psi-mi:"MI:0819"(molecular weight estimation by bromide staining)