#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P49177 uniprotkb:Q9FDX9 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-1750878|uniprotkb:Q9LY73|ensemblplants:AT3G63420.1|ensemblplants:AT3G63420.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gg1_arath(display_long)|uniprotkb:GG1(gene name)|psi-mi:GG1(display_short)|uniprotkb:AGG1(gene name synonym)|uniprotkb:Ggamma-subunit 1(gene name synonym)|uniprotkb:Heterotrimeric G protein gamma-subunit 1(gene name synonym)|uniprotkb:MAA21.50(orf name)|uniprotkb:At3g63420(locus name) psi-mi:"MI:0663"(confocal microscopy) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0403"(colocalization) psi-mi:"MI:0469"(IntAct) intact:EBI-1750883|imex:IM-20036-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 refseq:NP_567147.1|refseq:NP_850741.1|interpro:IPR015898(G-protein, gamma-like subunit)|refseq:NP_001325615.1|ensemblplants:AT3G63420(gene)|ensemblplants:AT3G63420.1(transcript)|ensemblplants:AT3G63420.2(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010541"(acropetal auxin transport)|go:"GO:0018342"(protein prenylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus) - - - figure legend:Figure 2|comment:Localisation of GB1 to the plasma membrane was dependent on the presence of AGG1.|expression level:Q9FDX9 over-expressed|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:DfRvk4VTDrv1qRnVQCkwuleNNaA3702 intact-crc:D66917834134093D|rigid:DDBTiIyaNoZmJP7ElK0075MWSe0 false yellow fluorescent protein tag:n-n - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P49177 uniprotkb:Q9FDX9 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-1750878|uniprotkb:Q9LY73|ensemblplants:AT3G63420.1|ensemblplants:AT3G63420.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gg1_arath(display_long)|uniprotkb:GG1(gene name)|psi-mi:GG1(display_short)|uniprotkb:AGG1(gene name synonym)|uniprotkb:Ggamma-subunit 1(gene name synonym)|uniprotkb:Heterotrimeric G protein gamma-subunit 1(gene name synonym)|uniprotkb:MAA21.50(orf name)|uniprotkb:At3g63420(locus name) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-1750901|imex:IM-20036-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 refseq:NP_567147.1|refseq:NP_850741.1|interpro:IPR015898(G-protein, gamma-like subunit)|refseq:NP_001325615.1|ensemblplants:AT3G63420(gene)|ensemblplants:AT3G63420.1(transcript)|ensemblplants:AT3G63420.2(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010541"(acropetal auxin transport)|go:"GO:0018342"(protein prenylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus) - - - figure legend:Figure 3A|comment:Colocalisation occurs in the plasma membrane.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:DfRvk4VTDrv1qRnVQCkwuleNNaA3702 intact-crc:FA0BB6BCFE11CFF2|rigid:DDBTiIyaNoZmJP7ElK0075MWSe0 false cyan fluorescent protein tag:n-n yellow fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P49177 uniprotkb:Q9FDX9 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-1750878|uniprotkb:Q9LY73|ensemblplants:AT3G63420.1|ensemblplants:AT3G63420.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gg1_arath(display_long)|uniprotkb:GG1(gene name)|psi-mi:GG1(display_short)|uniprotkb:AGG1(gene name synonym)|uniprotkb:Ggamma-subunit 1(gene name synonym)|uniprotkb:Heterotrimeric G protein gamma-subunit 1(gene name synonym)|uniprotkb:MAA21.50(orf name)|uniprotkb:At3g63420(locus name) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-1750911|imex:IM-20036-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 refseq:NP_567147.1|refseq:NP_850741.1|interpro:IPR015898(G-protein, gamma-like subunit)|refseq:NP_001325615.1|ensemblplants:AT3G63420(gene)|ensemblplants:AT3G63420.1(transcript)|ensemblplants:AT3G63420.2(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010541"(acropetal auxin transport)|go:"GO:0018342"(protein prenylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus) - - - figure legend:Figure 3D Figure 3I|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors state that \"the G alpha subunit does not interact with either G beta or G gamma1 alone in protoplasts, but interacts with the G beta /gamma complex\"."|expression level:P18064 over-expressed|expression level:P49177 over-expressed|expression level:Q9FDX9 over-expressed|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:DfRvk4VTDrv1qRnVQCkwuleNNaA3702 intact-crc:CAF82C810D54E251|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false yellow fluorescent protein tag:n-n yellow fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P49177 uniprotkb:P18064 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-1750911|imex:IM-20036-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N - - - figure legend:Figure 3D Figure 3I|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors state that \"the G alpha subunit does not interact with either G beta or G gamma1 alone in protoplasts, but interacts with the G beta /gamma complex\"."|expression level:P18064 over-expressed|expression level:P49177 over-expressed|expression level:Q9FDX9 over-expressed|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 intact-crc:CAF82C810D54E251|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false yellow fluorescent protein tag:n-n cyan fluorescent protein tag:97-98 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P49177 uniprotkb:Q9FDX9 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-1750878|uniprotkb:Q9LY73|ensemblplants:AT3G63420.1|ensemblplants:AT3G63420.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gg1_arath(display_long)|uniprotkb:GG1(gene name)|psi-mi:GG1(display_short)|uniprotkb:AGG1(gene name synonym)|uniprotkb:Ggamma-subunit 1(gene name synonym)|uniprotkb:Heterotrimeric G protein gamma-subunit 1(gene name synonym)|uniprotkb:MAA21.50(orf name)|uniprotkb:At3g63420(locus name) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-1751517|imex:IM-20036-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 refseq:NP_567147.1|refseq:NP_850741.1|interpro:IPR015898(G-protein, gamma-like subunit)|refseq:NP_001325615.1|ensemblplants:AT3G63420(gene)|ensemblplants:AT3G63420.1(transcript)|ensemblplants:AT3G63420.2(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010541"(acropetal auxin transport)|go:"GO:0018342"(protein prenylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus) - - - figure legend:Figure 3F Figure 3I|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors concluded that \"the G alpha subunit does not interact with either Gbeta or G gamma1 alone in protoplasts, but interacts with the G beta/gamma complex.\"."|expression level:P18064 over-expressed|expression level:P49177 over-expressed|expression level:Q9FDX9 over-expressed|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:DfRvk4VTDrv1qRnVQCkwuleNNaA3702 intact-crc:584EE06FBA92FDE9|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false - yellow fluorescent protein tag:n-n - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P49177 uniprotkb:P18064 intact:EBI-1632851|ensemblplants:AT4G34460.1 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:"MI:0055"(fluorescent resonance energy transfer) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-1751517|imex:IM-20036-5 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N - - - figure legend:Figure 3F Figure 3I|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors concluded that \"the G alpha subunit does not interact with either Gbeta or G gamma1 alone in protoplasts, but interacts with the G beta/gamma complex.\"."|expression level:P18064 over-expressed|expression level:P49177 over-expressed|expression level:Q9FDX9 over-expressed|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 intact-crc:584EE06FBA92FDE9|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false - cyan fluorescent protein tag:97-98 - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence) uniprotkb:P18064 uniprotkb:P49177 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 intact:EBI-1632851|ensemblplants:AT4G34460.1 psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:gbb_arath(display_long)|uniprotkb:GB1(gene name)|psi-mi:GB1(display_short)|uniprotkb:T4L20.40(orf name)|uniprotkb:At4g34460(locus name)|uniprotkb:transducin(gene name synonym)|uniprotkb:AGB1(gene name synonym) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-1750932|imex:IM-20036-4 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0684"(ancillary) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N dip:DIP-40108N|refseq:NP_195172.1|ensemblplants:AT4G34460(gene)|ensemblplants:AT4G34460.1(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009723"(response to ethylene)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009867"(jasmonic acid mediated signaling pathway)|go:"GO:0009991"(response to extracellular stimulus)|go:"GO:0010118"(stomatal movement)|go:"GO:0010154"(fruit development)|go:"GO:0030968"(endoplasmic reticulum unfolded protein response)|go:"GO:0031682"(G-protein gamma-subunit binding)|go:"GO:0048364"(root development)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|go:"GO:1905392"(plant organ morphogenesis)|go:"GO:2000280"(regulation of root development)|interpro:IPR001632(G-protein, beta subunit)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR016346(Guanine nucleotide-binding protein, beta subunit)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322 - - - figure legend:Figure 4|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors state that \" the subunits of the Arabidopsis G protein form a heterotrimeric complex in vivo and that the interaction of G alpha and G gamma 1 requires G beta\"."|expression level:Q9FDX9 over-expressed|expression level:P49177 over-expressed|expression level:P18064 over-expressed|caution:Protein indentification was made on the basis of protein size using electrophoresis through a 12 percent discontinuous polyacrylamide gel.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 rogid:WstY3eSNpOHBxk7+tDfYLgm3J3A3702 intact-crc:F3DDA1BD8C1511D3|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false cyan fluorescent protein tag:97-98 - - - psi-mi:"MI:0815"(confirmation by molecular weight) psi-mi:"MI:0815"(confirmation by molecular weight) uniprotkb:P18064 uniprotkb:Q9FDX9 intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2 intact:EBI-1750878|uniprotkb:Q9LY73|ensemblplants:AT3G63420.1|ensemblplants:AT3G63420.2 psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name) psi-mi:gg1_arath(display_long)|uniprotkb:GG1(gene name)|psi-mi:GG1(display_short)|uniprotkb:AGG1(gene name synonym)|uniprotkb:Ggamma-subunit 1(gene name synonym)|uniprotkb:Heterotrimeric G protein gamma-subunit 1(gene name synonym)|uniprotkb:MAA21.50(orf name)|uniprotkb:At3g63420(locus name) psi-mi:"MI:0006"(anti bait coimmunoprecipitation) Wang et al. (2008) pubmed:18326491|imex:IM-20036 taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)") psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-1750932|imex:IM-20036-4 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N refseq:NP_567147.1|refseq:NP_850741.1|interpro:IPR015898(G-protein, gamma-like subunit)|refseq:NP_001325615.1|ensemblplants:AT3G63420(gene)|ensemblplants:AT3G63420.1(transcript)|ensemblplants:AT3G63420.2(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010541"(acropetal auxin transport)|go:"GO:0018342"(protein prenylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0048527"(lateral root development)|go:"GO:0050832"(defense response to fungus) - - - figure legend:Figure 4|comment:The cfp tag was positioned between amino acid residues 97 and 98 in the GPA1 protein|comment:"The authors state that \" the subunits of the Arabidopsis G protein form a heterotrimeric complex in vivo and that the interaction of G alpha and G gamma 1 requires G beta\"."|expression level:Q9FDX9 over-expressed|expression level:P49177 over-expressed|expression level:P18064 over-expressed|caution:Protein indentification was made on the basis of protein size using electrophoresis through a 12 percent discontinuous polyacrylamide gel.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf) - 2008/04/09 2014/10/16 rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702 rogid:DfRvk4VTDrv1qRnVQCkwuleNNaA3702 intact-crc:F3DDA1BD8C1511D3|rigid:Ynj+9JJDc8CFbk8YAxoQeSf6yt0 false cyan fluorescent protein tag:97-98 yellow fluorescent protein tag:n-n - - psi-mi:"MI:0815"(confirmation by molecular weight) psi-mi:"MI:0815"(confirmation by molecular weight)