#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P18064	uniprotkb:Q38882	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-962294|ensemblplants:AT3G15730.1|uniprotkb:Q944M4|uniprotkb:Q9LW06|uniprotkb:Q0WV84	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:plda1_arath(display_long)|uniprotkb:PLDALPHA1(gene name)|psi-mi:PLDALPHA1(display_short)|uniprotkb:PLD1(gene name synonym)|uniprotkb:At3g15730(locus name)|uniprotkb:MSJ11.13(orf name)|uniprotkb:Choline phosphatase 1(gene name synonym)|uniprotkb:Phosphatidylcholine-hydrolyzing phospholipase D 1(gene name synonym)|uniprotkb:PLDalpha(gene name synonym)	psi-mi:"MI:0096"(pull down)	Zhao et al. (2004)	pubmed:14594812|imex:IM-19317	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1751206|imex:IM-19317-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	ensemblplants:AT3G15730(gene)|ensemblplants:AT3G15730.1(transcript)|go:"GO:0004620"(phospholipase activity)|go:"GO:0004630"(phospholipase D activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005773"(vacuole)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006631"(fatty acid metabolic process)|go:"GO:0009395"(phospholipid catabolic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009507"(chloroplast)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009873"(ethylene-activated signaling pathway)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010358"(leaf shaping)|go:"GO:0016020"(membrane)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0046470"(phosphatidylcholine metabolic process)|go:"GO:0046686"(response to cadmium ion)|go:"GO:0070290"(N-acylphosphatidylethanolamine-specific phospholipase D activity)|refseq:NP_188194.1|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR015679(Phospholipase D)|interpro:IPR001736(Phospholipase D/Transphosphatidylase)|interpro:IPR024632|interpro:IPR011402(Phospholipase D, plant)|interpro:IPR035892|rcsb pdb:6KZ8|rcsb pdb:6KZ9	-	-	-	figure legend:Figure 2 Figure 4|comment:"The authors state that \"To estimate the stoichiometry of the interaction, G{alpha} and PLD{alpha}1 were purified and co-incubated at different molar ratios (Fig. 2B). Increasing the ratio of PLD{alpha}1 to G{alpha} from 0.5 to 1 led to a doubling of the PLD co-precipitated with G{alpha}, whereas an increase in the molar ratio to 2:1 did not lead to a further increase in PLD{alpha} complexed with G{alpha}. These results indicate that PLD{alpha}1 binds to G{alpha} in a 1:1 ratio\"."|comment:"From the data presented in Figure 4 the authors conclude that \"the residue Lys564 is essential for PLD{alpha}1 to interact with G{alpha}, whereas the flanking Glu563 and Phe565 are important for enhancing this interaction\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:511693(ecobb)|taxid:511693("Escherichia coli (strain B / BL21)")	-	2008/04/10	2014/10/16	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:lgWOTaG2ewnvDRp8dDqhqvVd3F43702	intact-crc:3C194D90115C1DBD|rigid:/CMwCULa00aEMkI3UJetGgt5ZAQ	false	glutathione s tranferase tag:n-n	his tag:n-n|mutation decreasing interaction:563-563|mutation disrupting interaction:564-564|mutation decreasing interaction:565-565	1	1	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P18064	uniprotkb:Q38882	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-962294|ensemblplants:AT3G15730.1|uniprotkb:Q944M4|uniprotkb:Q9LW06|uniprotkb:Q0WV84	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:plda1_arath(display_long)|uniprotkb:PLDALPHA1(gene name)|psi-mi:PLDALPHA1(display_short)|uniprotkb:PLD1(gene name synonym)|uniprotkb:At3g15730(locus name)|uniprotkb:MSJ11.13(orf name)|uniprotkb:Choline phosphatase 1(gene name synonym)|uniprotkb:Phosphatidylcholine-hydrolyzing phospholipase D 1(gene name synonym)|uniprotkb:PLDalpha(gene name synonym)	psi-mi:"MI:0096"(pull down)	Zhao et al. (2004)	pubmed:14594812|imex:IM-19317	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1751224|imex:IM-19317-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	ensemblplants:AT3G15730(gene)|ensemblplants:AT3G15730.1(transcript)|go:"GO:0004620"(phospholipase activity)|go:"GO:0004630"(phospholipase D activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005773"(vacuole)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006631"(fatty acid metabolic process)|go:"GO:0009395"(phospholipid catabolic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009507"(chloroplast)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009873"(ethylene-activated signaling pathway)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010358"(leaf shaping)|go:"GO:0016020"(membrane)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0046470"(phosphatidylcholine metabolic process)|go:"GO:0046686"(response to cadmium ion)|go:"GO:0070290"(N-acylphosphatidylethanolamine-specific phospholipase D activity)|refseq:NP_188194.1|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR015679(Phospholipase D)|interpro:IPR001736(Phospholipase D/Transphosphatidylase)|interpro:IPR024632|interpro:IPR011402(Phospholipase D, plant)|interpro:IPR035892|rcsb pdb:6KZ8|rcsb pdb:6KZ9	-	-	-	figure legend:Figure 2 Figure 4|comment:"The authors state that \"To estimate the stoichiometry of the interaction, G{alpha} and PLD{alpha}1 were purified and co-incubated at different molar ratios (Fig. 2B). Increasing the ratio of PLD{alpha}1 to G{alpha} from 0.5 to 1 led to a doubling of the PLD co-precipitated with G{alpha}, whereas an increase in the molar ratio to 2:1 did not lead to a further increase in PLD{alpha} complexed with G{alpha}. These results indicate that PLD{alpha}1 binds to G{alpha} in a 1:1 ratio\"."|comment:"From the data presented in Figure 4 the authors conclude that \"the residue Lys564 is essential for PLD{alpha}1 to interact with G{alpha}, whereas the flanking Glu563 and Phe565 are important for enhancing this interaction\"."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:511693(ecobb)|taxid:511693("Escherichia coli (strain B / BL21)")	-	2008/04/10	2014/10/16	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:lgWOTaG2ewnvDRp8dDqhqvVd3F43702	intact-crc:503EC743E7EE5D53|rigid:/CMwCULa00aEMkI3UJetGgt5ZAQ	false	glutathione s tranferase tag:n-n	his tag:n-n|mutation decreasing interaction:563-563|mutation decreasing interaction:564-564|mutation decreasing interaction:565-565	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P18064	uniprotkb:Q38882	intact:EBI-443890|ensemblplants:AT2G26300.1|ensemblplants:AT2G26300.2	intact:EBI-962294|ensemblplants:AT3G15730.1|uniprotkb:Q944M4|uniprotkb:Q9LW06|uniprotkb:Q0WV84	psi-mi:gpa1_arath(display_long)|uniprotkb:GPA1(gene name)|psi-mi:GPA1(display_short)|uniprotkb:At2g26300(locus name)|uniprotkb:T1D16.6(orf name)	psi-mi:plda1_arath(display_long)|uniprotkb:PLDALPHA1(gene name)|psi-mi:PLDALPHA1(display_short)|uniprotkb:PLD1(gene name synonym)|uniprotkb:At3g15730(locus name)|uniprotkb:MSJ11.13(orf name)|uniprotkb:Choline phosphatase 1(gene name synonym)|uniprotkb:Phosphatidylcholine-hydrolyzing phospholipase D 1(gene name synonym)|uniprotkb:PLDalpha(gene name synonym)	psi-mi:"MI:0096"(pull down)	Zhao et al. (2004)	pubmed:14594812|imex:IM-19317	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	taxid:3702(arath)|taxid:3702("Arabidopsis thaliana (Mouse-ear cress)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1751248|imex:IM-19317-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblplants:AT2G26300(gene)|ensemblplants:AT2G26300.1(transcript)|ensemblplants:AT2G26300.2(transcript)|go:"GO:0001664"(G protein-coupled receptor binding)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005777"(peroxisome)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0006571"(tyrosine biosynthetic process)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007188"(adenylate cyclase-modulating G protein-coupled receptor signaling pathway)|go:"GO:0008219"(cell death)|go:"GO:0009094"(L-phenylalanine biosynthetic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009740"(gibberellic acid mediated signaling pathway)|go:"GO:0009749"(response to glucose)|go:"GO:0009785"(blue light signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0010027"(thylakoid membrane organization)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010244"(response to low fluence blue light stimulus by blue low-fluence system)|go:"GO:0016247"(channel regulator activity)|go:"GO:0019236"(response to pheromone)|go:"GO:0031683"(G-protein beta/gamma-subunit complex binding)|go:"GO:0042127"(regulation of cell population proliferation)|go:"GO:0046872"(metal ion binding)|go:"GO:0051020"(GTPase binding)|go:"GO:0072593"(reactive oxygen species metabolic process)|go:"GO:0090333"(regulation of stomatal closure)|interpro:IPR001019("Guanine nucleotide binding protein (G-protein), alpha subunit")|interpro:IPR011025(G protein alpha subunit, helical insertion)|interpro:IPR027417|rcsb pdb:2XTZ|interpro:IPR002976(Plant G-protein, alpha subunit)|refseq:NP_180198.1|refseq:NP_001325056.1|mint:P18064|dip:DIP-31793N	ensemblplants:AT3G15730(gene)|ensemblplants:AT3G15730.1(transcript)|go:"GO:0004620"(phospholipase activity)|go:"GO:0004630"(phospholipase D activity)|go:"GO:0005096"(GTPase activator activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005546"(phosphatidylinositol-4,5-bisphosphate binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005773"(vacuole)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006631"(fatty acid metabolic process)|go:"GO:0009395"(phospholipid catabolic process)|go:"GO:0009506"(plasmodesma)|go:"GO:0009507"(chloroplast)|go:"GO:0009737"(response to abscisic acid)|go:"GO:0009738"(abscisic acid-activated signaling pathway)|go:"GO:0009789"(positive regulation of abscisic acid-activated signaling pathway)|go:"GO:0009845"(seed germination)|go:"GO:0009873"(ethylene-activated signaling pathway)|go:"GO:0010119"(regulation of stomatal movement)|go:"GO:0010358"(leaf shaping)|go:"GO:0016020"(membrane)|go:"GO:0030136"(clathrin-coated vesicle)|go:"GO:0031966"(mitochondrial membrane)|go:"GO:0046470"(phosphatidylcholine metabolic process)|go:"GO:0046686"(response to cadmium ion)|go:"GO:0070290"(N-acylphosphatidylethanolamine-specific phospholipase D activity)|refseq:NP_188194.1|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR015679(Phospholipase D)|interpro:IPR001736(Phospholipase D/Transphosphatidylase)|interpro:IPR024632|interpro:IPR011402(Phospholipase D, plant)|interpro:IPR035892|rcsb pdb:6KZ8|rcsb pdb:6KZ9	-	-	-	figure legend:Figure 3|comment:"The authors state \"the amount of PLD{alpha}1 co-precipitated with G{alpha} increased as the amount of G{alpha} was increased (Fig. 3), indicating that the G{alpha} and PLD{alpha}1 binding is dose-dependent\"."|comment:The identification was confirmed by enzymic assay as described in the text.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:3702(arath-leaf)|taxid:3702(Arabidopsis thaliana leaf)	-	2008/04/10	2014/10/16	rogid:BngFnsb4kUhGWFZ7x1OT1AeX94I3702	rogid:lgWOTaG2ewnvDRp8dDqhqvVd3F43702	intact-crc:4AA844AF73775266|rigid:/CMwCULa00aEMkI3UJetGgt5ZAQ	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)