#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P40559	uniprotkb:P47030	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	intact:EBI-25970|uniprotkb:D6VWA0|ensemblfungi:YJL083W	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:tax4_yeast(display_long)|uniprotkb:TAX4(gene name)|psi-mi:TAX4(display_short)|uniprotkb:J1002(orf name)|uniprotkb:YJL083W(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534735|imex:IM-19749-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	refseq:NP_012452.1|sgd:S000003619|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|ensemblfungi:YJL083W(gene)|ensemblfungi:YJL083W_mRNA(transcript)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0048017"(inositol lipid-mediated signaling)	-	-	-	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	rogid:q2x8QHytzG7WIcTj0u3OZawCyTo559292	intact-crc:C99643EC8A0D4E0C|rigid:hrWveVHprzDe95x5ehSXjtL8/os	false	ha tag:?-?	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:P40559	uniprotkb:P36115	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	intact:EBI-27090|uniprotkb:D6VX84|ensemblfungi:YKR019C	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:irs4_yeast(display_long)|uniprotkb:IRS4(gene name)|psi-mi:IRS4(display_short)|uniprotkb:YKR019C(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534735|imex:IM-19749-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	sgd:S000001727|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|refseq:NP_012944.3|ensemblfungi:YKR019C(gene)|ensemblfungi:YKR019C_mRNA(transcript)|go:"GO:0000183"(rDNA heterochromatin assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0005739"(mitochondrion)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016197"(endosomal transport)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048017"(inositol lipid-mediated signaling)	-	-	-	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	rogid:YpvcNjiACi98c6YwI7+fdB7ujs0559292	intact-crc:C99643EC8A0D4E0C|rigid:hrWveVHprzDe95x5ehSXjtL8/os	false	ha tag:?-?	-	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:P40559	uniprotkb:P47030	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	intact:EBI-25970|uniprotkb:D6VWA0|ensemblfungi:YJL083W	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:tax4_yeast(display_long)|uniprotkb:TAX4(gene name)|psi-mi:TAX4(display_short)|uniprotkb:J1002(orf name)|uniprotkb:YJL083W(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534748|imex:IM-19749-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	refseq:NP_012452.1|sgd:S000003619|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|ensemblfungi:YJL083W(gene)|ensemblfungi:YJL083W_mRNA(transcript)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0048017"(inositol lipid-mediated signaling)	-	-	-	figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	rogid:q2x8QHytzG7WIcTj0u3OZawCyTo559292	intact-crc:A147D809E68373F9|rigid:R7GU2zA0sH68qUf39hE4ng+NBRA	false	ha tag:?-?	myc tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P47030	uniprotkb:P40559	intact:EBI-25970|uniprotkb:D6VWA0|ensemblfungi:YJL083W	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	psi-mi:tax4_yeast(display_long)|uniprotkb:TAX4(gene name)|psi-mi:TAX4(display_short)|uniprotkb:J1002(orf name)|uniprotkb:YJL083W(locus name)	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534759|imex:IM-19749-3	-	-	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_012452.1|sgd:S000003619|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|ensemblfungi:YJL083W(gene)|ensemblfungi:YJL083W_mRNA(transcript)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0048017"(inositol lipid-mediated signaling)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	-	-	-	figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:q2x8QHytzG7WIcTj0u3OZawCyTo559292	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	intact-crc:934AC4001D722ECD|rigid:R7GU2zA0sH68qUf39hE4ng+NBRA	false	myc tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P36115	uniprotkb:P40559	intact:EBI-27090|uniprotkb:D6VX84|ensemblfungi:YKR019C	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	psi-mi:irs4_yeast(display_long)|uniprotkb:IRS4(gene name)|psi-mi:IRS4(display_short)|uniprotkb:YKR019C(locus name)	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534767|imex:IM-19749-4	-	-	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000001727|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|refseq:NP_012944.3|ensemblfungi:YKR019C(gene)|ensemblfungi:YKR019C_mRNA(transcript)|go:"GO:0000183"(rDNA heterochromatin assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0005739"(mitochondrion)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016197"(endosomal transport)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048017"(inositol lipid-mediated signaling)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	-	-	-	figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:YpvcNjiACi98c6YwI7+fdB7ujs0559292	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	intact-crc:B8D2ED02BF146264|rigid:44wjdMQUp73NJg9IUFWJ+AqRyHw	false	myc tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P36115	uniprotkb:P40559	intact:EBI-27090|uniprotkb:D6VX84|ensemblfungi:YKR019C	intact:EBI-24915|uniprotkb:D6VVS9|ensemblfungi:YIL002C	psi-mi:irs4_yeast(display_long)|uniprotkb:IRS4(gene name)|psi-mi:IRS4(display_short)|uniprotkb:YKR019C(locus name)	psi-mi:inp51_yeast(display_long)|uniprotkb:YIL002C(locus name)|uniprotkb:YIA2C(orf name)|uniprotkb:INP51(gene name)|psi-mi:INP51(display_short)|uniprotkb:SJL1(gene name synonym)|uniprotkb:Synaptojanin-like protein 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Morales-Johansson et al. (2004)	pubmed:15265867|imex:IM-19749	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0486"(UniProt)	intact:EBI-1534780|imex:IM-19749-5	-	-	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000001727|interpro:IPR011992(EF-Hand type)|interpro:IPR000261("EPS15 homology (EH)")|refseq:NP_012944.3|ensemblfungi:YKR019C(gene)|ensemblfungi:YKR019C_mRNA(transcript)|go:"GO:0000183"(rDNA heterochromatin assembly)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0005739"(mitochondrion)|go:"GO:0006629"(lipid metabolic process)|go:"GO:0006897"(endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0009267"(cellular response to starvation)|go:"GO:0016197"(endosomal transport)|go:"GO:0031505"(fungal-type cell wall organization)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0048017"(inositol lipid-mediated signaling)	sgd:S000001264|refseq:NP_012264.3|mint:P40559|dip:DIP-2677N|ensemblfungi:YIL002C(gene)|ensemblfungi:YIL002C_mRNA(transcript)|go:"GO:0004439"(phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0006897"(endocytosis)|go:"GO:0015031"(protein transport)|go:"GO:0016020"(membrane)|go:"GO:0030479"(actin cortical patch)|go:"GO:0043813"(phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity)|go:"GO:0046855"(inositol phosphate dephosphorylation)|go:"GO:0046856"(phosphatidylinositol dephosphorylation)|interpro:IPR000300(Inositol polyphosphate related phosphatase)|interpro:IPR002013(Synaptojanin, N-terminal)|interpro:IPR005135(Endonuclease/exonuclease/phosphatase)|interpro:IPR036691|reactome:R-SCE-1660499|reactome:R-SCE-8856828	-	-	-	figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2007/09/20	2014/10/16	rogid:YpvcNjiACi98c6YwI7+fdB7ujs0559292	rogid:rIU7NYsMpt3GUXBf0jfAW7QyVxQ559292	intact-crc:8ADFF10B44E53F50|rigid:44wjdMQUp73NJg9IUFWJ+AqRyHw	false	myc tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)