#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P69741	uniprotkb:P0AAN1	intact:EBI-552619|uniprotkb:Q2M9J8|uniprotkb:Q46847|ensemblbacteria:AAC76033|ensemblbacteria:BAE77058	intact:EBI-552588|uniprotkb:P37183|uniprotkb:Q2M9K3|ensemblbacteria:AAC76028|ensemblbacteria:BAE77053	psi-mi:mbht_ecoli(display_long)|uniprotkb:yghV(gene name synonym)|uniprotkb:Membrane-bound hydrogenase 2 small subunit(gene name synonym)|uniprotkb:NiFe hydrogenase(gene name synonym)|uniprotkb:hybO(gene name)|psi-mi:hybO(display_short)|uniprotkb:b2997(locus name)|uniprotkb:JW2965(locus name)	psi-mi:hybe_ecoli(display_long)|uniprotkb:hybE(gene name)|psi-mi:hybE(display_short)|uniprotkb:b2992(locus name)|uniprotkb:JW2960(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-850883	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417471.1|refseq:WP_000145410.1|ensemblbacteria:AAC76033(transcript)|ensemblbacteria:b2997(gene)|ensemblbacteria:BAE77058(gene)|ensemblbacteria:BAE77058(transcript)|go:"GO:0008901"(ferredoxin hydrogenase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0009375"(ferredoxin hydrogenase complex)|go:"GO:0016020"(membrane)|go:"GO:0031236"(extrinsic component of periplasmic side of plasma membrane)|go:"GO:0033748"("hydrogenase (acceptor) activity")|go:"GO:0042597"(periplasmic space)|go:"GO:0044569"([Ni-Fe] hydrogenase complex)|go:"GO:0046872"(metal ion binding)|go:"GO:0051536"(iron-sulfur cluster binding)|go:"GO:0051538"(3 iron, 4 sulfur cluster binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001821([Ni-Fe]-hydrogenase, small subunit)|interpro:IPR006137(NADH ubiquinone oxidoreductase, 20 kDa subunit)|interpro:IPR006311(Twin-arginine translocation pathway signal)|interpro:IPR019546|interpro:IPR027394|interpro:IPR037024|interpro:IPR037148|rcsb pdb:6EHQ|rcsb pdb:6EHS|rcsb pdb:6EN9|rcsb pdb:6G7M|rcsb pdb:6GAM|rcsb pdb:6GAN|rcsb pdb:6SYO|rcsb pdb:6SYX|rcsb pdb:6SZD|rcsb pdb:6SZK|dip:DIP-36024N	refseq:NP_417466.1|refseq:WP_000134014.1|dip:DIP-35795N|ensemblbacteria:AAC76028(transcript)|ensemblbacteria:b2992(gene)|ensemblbacteria:BAE77053(gene)|ensemblbacteria:BAE77053(transcript)|go:"GO:0051604"(protein maturation)|go:"GO:0070678"(preprotein binding)|interpro:IPR023994|interpro:IPR038530|rcsb pdb:2KC5	-	crc64:C5821096BBAF9C66	crc64:CFDAEA3D47BE9516	expression level:P0AAN1 endogenous	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:q3mPMVF7Qd4HSkzFGtPodT7/4Bo83333	rogid:ygiFIogsROT5Sqp8Cc4yI4dELTI83333	intact-crc:6B120770D3D60540|rigid:fki+YUT0d+OuU/nTq5q1mvrDDJc	false	-	tag:c-c	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0A9K9	uniprotkb:P0AAN3	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851117	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	-	-	-	expression level:P0AAN3 endogenous	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	intact-crc:B18660442055DAFA|rigid:CNoLraNO1+znvzt7cHcVW0z/mQ4	false	-	tag:c-c	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAN3	uniprotkb:P0A9K9	intact:EBI-558261|uniprotkb:P24190|uniprotkb:Q2MAA2|uniprotkb:Q46884|ensemblbacteria:AAC75769|ensemblbacteria:BAE76804	intact:EBI-369251|uniprotkb:P30856|uniprotkb:Q2M714|ensemblbacteria:AAC76374|ensemblbacteria:BAE77942	psi-mi:hypb_ecoli(display_long)|uniprotkb:hypB(gene name)|psi-mi:hypB(display_short)|uniprotkb:Hydrogenase isoenzymes nickel incorporation protein HypB(gene name synonym)|uniprotkb:JW2697(locus name)|uniprotkb:b2727(locus name)	psi-mi:slyd_ecoli(display_long)|uniprotkb:slyD(gene name)|psi-mi:slyD(display_short)|uniprotkb:b3349(locus name)|uniprotkb:Metallochaperone SlyD(gene name synonym)|uniprotkb:JW3311(locus name)|uniprotkb:Histidine-rich protein(gene name synonym)|uniprotkb:WHP(gene name synonym)|uniprotkb:Rotamase(gene name synonym)|uniprotkb:Sensitivity to lysis protein D(gene name synonym)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851353	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417207.1|refseq:WP_000337665.1|dip:DIP-36428N|ensemblbacteria:AAC75769(transcript)|ensemblbacteria:b2727(gene)|ensemblbacteria:BAE76804(gene)|ensemblbacteria:BAE76804(transcript)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016151"(nickel cation binding)|go:"GO:0051604"(protein maturation)|interpro:IPR003495("Cobalamin (vitamin B12) biosynthesis CobW-like")|interpro:IPR004392(Hydrogenase accessory protein HypB)|interpro:IPR027417	refseq:NP_417808.1|refseq:WP_000861334.1|ensemblbacteria:AAC76374(transcript)|ensemblbacteria:b3349(gene)|ensemblbacteria:BAE77942(gene)|ensemblbacteria:BAE77942(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009408"(response to heat)|go:"GO:0016151"(nickel cation binding)|go:"GO:0022417"(protein maturation by protein folding)|go:"GO:0042026"(protein refolding)|go:"GO:0043963"(modulation by symbiont of host adenylate cyclase-mediated signal transduction)|go:"GO:0050821"(protein stabilization)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0051082"(unfolded protein binding)|go:"GO:0051604"(protein maturation)|interpro:IPR001179(Peptidyl-prolyl cis-trans isomerase, FKBP-type)|rcsb pdb:2K8I|rcsb pdb:2KFW|rcsb pdb:5I7P|dip:DIP-31853N	-	-	-	expression level:P0A9K9 endogenous	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:bg58XWiAv28fGfbHi898yF5HN8o83333	rogid:Y/b//pPxL6aoUOf5NH2HKiB+wN883333	intact-crc:9326545002884187|rigid:CNoLraNO1+znvzt7cHcVW0z/mQ4	false	-	tag:c-c	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM7	uniprotkb:P24193	intact:EBI-562426|ensemblbacteria:AAC76026|ensemblbacteria:BAE77051|uniprotkb:P37185|uniprotkb:Q2M9K5	intact:EBI-552743|uniprotkb:Q2MA99|uniprotkb:Q46886|ensemblbacteria:AAC75772|ensemblbacteria:BAE76807	psi-mi:hybg_ecoli(display_long)|uniprotkb:hybG(gene name)|psi-mi:hybG(display_short)|uniprotkb:Hydrogenase-2 operon protein HybG(gene name synonym)|uniprotkb:JW2958(locus name)|uniprotkb:b2990(locus name)	psi-mi:hype_ecoli(display_long)|uniprotkb:hypE(gene name)|psi-mi:hypE(display_short)|uniprotkb:Hydrogenase maturation factor HypE(gene name synonym)|uniprotkb:JW2700(locus name)|uniprotkb:b2730(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851486	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC76026(transcript)|ensemblbacteria:b2990(gene)|ensemblbacteria:BAE77051(gene)|ensemblbacteria:BAE77051(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|refseq:NP_417464.1|refseq:WP_000334896.1|dip:DIP-36429N	refseq:NP_417210.2|refseq:WP_001059922.1|dip:DIP-9999N|ensemblbacteria:AAC75772(transcript)|ensemblbacteria:b2730(gene)|ensemblbacteria:BAE76807(gene)|ensemblbacteria:BAE76807(transcript)|go:"GO:0016829"(lyase activity)|go:"GO:0046892"(peptidyl-S-carbamoyl-L-cysteine dehydration)|go:"GO:0051604"(protein maturation)|interpro:IPR010918(AIR synthase related protein, C-terminal)|interpro:IPR011854(Hydrogenase expression/formation protein HypE)|interpro:IPR016188(PurM, N-terminal-like)|interpro:IPR036676|interpro:IPR036921|rcsb pdb:2I6R	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	comment:HypD and HypE need to form a complex prior to association with HybG. This was demonstrated by pull-down analysis in KO mutants either for HypD or for HypE.|expression level:P0AAM7 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located on the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:oxJtPSNfRXaJd8VilW/S7qxEpjg83333	rogid:ZeGMKm2MPic8eLKZhUNkxiXX80o83333	intact-crc:64C6C64905CF28BF|rigid:BxUNk1abPrZYSkyOlx7NdwSJdj0	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM7	uniprotkb:P0ACD8	intact:EBI-562426|ensemblbacteria:AAC76026|ensemblbacteria:BAE77051|uniprotkb:P37185|uniprotkb:Q2M9K5	intact:EBI-851493|uniprotkb:P19927|uniprotkb:P78056|ensemblbacteria:AAC74058|ensemblbacteria:BAA35738	psi-mi:hybg_ecoli(display_long)|uniprotkb:hybG(gene name)|psi-mi:hybG(display_short)|uniprotkb:Hydrogenase-2 operon protein HybG(gene name synonym)|uniprotkb:JW2958(locus name)|uniprotkb:b2990(locus name)	psi-mi:mbhl_ecoli(display_long)|uniprotkb:Membrane-bound hydrogenase 1 large subunit(gene name synonym)|uniprotkb:NiFe hydrogenase(gene name synonym)|uniprotkb:hyaB(gene name)|psi-mi:hyaB(display_short)|uniprotkb:b0973(locus name)|uniprotkb:JW0955(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851486	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC76026(transcript)|ensemblbacteria:b2990(gene)|ensemblbacteria:BAE77051(gene)|ensemblbacteria:BAE77051(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|refseq:NP_417464.1|refseq:WP_000334896.1|dip:DIP-36429N	refseq:NP_415492.1|refseq:WP_000107384.1|dip:DIP-36180N|ensemblbacteria:AAC74058(transcript)|ensemblbacteria:b0973(gene)|ensemblbacteria:BAA35738(gene)|ensemblbacteria:BAA35738(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0006113"(fermentation)|go:"GO:0008901"(ferredoxin hydrogenase activity)|go:"GO:0009055"(electron transfer activity)|go:"GO:0009061"(anaerobic respiration)|go:"GO:0016020"(membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0030288"(outer membrane-bounded periplasmic space)|go:"GO:0033748"("hydrogenase (acceptor) activity")|go:"GO:0044569"([Ni-Fe] hydrogenase complex)|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR018194|interpro:IPR029014|rcsb pdb:3UQY|rcsb pdb:3USC|rcsb pdb:3USE|rcsb pdb:4GD3|rcsb pdb:4UE3|rcsb pdb:5A4F|rcsb pdb:5A4I|rcsb pdb:5A4M|rcsb pdb:5ADU|rcsb pdb:5JRD|rcsb pdb:5LMM|rcsb pdb:5LRY|rcsb pdb:6FPI|rcsb pdb:6FPO|rcsb pdb:6FPW|rcsb pdb:6G7R|rcsb pdb:6G94|rcsb pdb:6GAL|rcsb pdb:6RP2	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	comment:HypD and HypE need to form a complex prior to association with HybG. This was demonstrated by pull-down analysis in KO mutants either for HypD or for HypE.|expression level:P0AAM7 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located on the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:oxJtPSNfRXaJd8VilW/S7qxEpjg83333	rogid:firEXZy87IzwBqxxSdMr2x7QvJs83333	intact-crc:64C6C64905CF28BF|rigid:BxUNk1abPrZYSkyOlx7NdwSJdj0	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM7	uniprotkb:P24192	intact:EBI-562426|ensemblbacteria:AAC76026|ensemblbacteria:BAE77051|uniprotkb:P37185|uniprotkb:Q2M9K5	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	psi-mi:hybg_ecoli(display_long)|uniprotkb:hybG(gene name)|psi-mi:hybG(display_short)|uniprotkb:Hydrogenase-2 operon protein HybG(gene name synonym)|uniprotkb:JW2958(locus name)|uniprotkb:b2990(locus name)	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851486	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC76026(transcript)|ensemblbacteria:b2990(gene)|ensemblbacteria:BAE77051(gene)|ensemblbacteria:BAE77051(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|refseq:NP_417464.1|refseq:WP_000334896.1|dip:DIP-36429N	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	comment:HypD and HypE need to form a complex prior to association with HybG. This was demonstrated by pull-down analysis in KO mutants either for HypD or for HypE.|expression level:P0AAM7 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located on the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:oxJtPSNfRXaJd8VilW/S7qxEpjg83333	rogid:wOc/noYpjxe923sUb/GJww3u77483333	intact-crc:64C6C64905CF28BF|rigid:BxUNk1abPrZYSkyOlx7NdwSJdj0	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM7	uniprotkb:P0ACE0	intact:EBI-562426|ensemblbacteria:AAC76026|ensemblbacteria:BAE77051|uniprotkb:P37185|uniprotkb:Q2M9K5	intact:EBI-549849|uniprotkb:P37181|uniprotkb:Q2M9K1|ensemblbacteria:AAC76030|ensemblbacteria:BAE77055	psi-mi:hybg_ecoli(display_long)|uniprotkb:hybG(gene name)|psi-mi:hybG(display_short)|uniprotkb:Hydrogenase-2 operon protein HybG(gene name synonym)|uniprotkb:JW2958(locus name)|uniprotkb:b2990(locus name)	psi-mi:mbhm_ecoli(display_long)|uniprotkb:hybC(gene name)|psi-mi:hybC(display_short)|uniprotkb:b2994(locus name)|uniprotkb:JW2962(locus name)|uniprotkb:Membrane-bound hydrogenase 2 large subunit(gene name synonym)|uniprotkb:NiFe hydrogenase(gene name synonym)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851486	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC76026(transcript)|ensemblbacteria:b2990(gene)|ensemblbacteria:BAE77051(gene)|ensemblbacteria:BAE77051(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|refseq:NP_417464.1|refseq:WP_000334896.1|dip:DIP-36429N	refseq:NP_417468.1|refseq:WP_000083065.1|ensemblbacteria:AAC76030(transcript)|ensemblbacteria:b2994(gene)|ensemblbacteria:BAE77055(gene)|ensemblbacteria:BAE77055(transcript)|go:"GO:0008901"(ferredoxin hydrogenase activity)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031236"(extrinsic component of periplasmic side of plasma membrane)|go:"GO:0033748"("hydrogenase (acceptor) activity")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR018194|interpro:IPR029014|rcsb pdb:6EHQ|rcsb pdb:6EHS|rcsb pdb:6EN9|rcsb pdb:6G7M|rcsb pdb:6GAM|rcsb pdb:6GAN|rcsb pdb:6SYO|dip:DIP-36022N	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	comment:HypD and HypE need to form a complex prior to association with HybG. This was demonstrated by pull-down analysis in KO mutants either for HypD or for HypE.|expression level:P0AAM7 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located on the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:oxJtPSNfRXaJd8VilW/S7qxEpjg83333	rogid:VPubvzOzdVl1a7bxWwnLl5p+kJY83333	intact-crc:64C6C64905CF28BF|rigid:BxUNk1abPrZYSkyOlx7NdwSJdj0	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P24192	uniprotkb:P24193	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	intact:EBI-552743|uniprotkb:Q2MA99|uniprotkb:Q46886|ensemblbacteria:AAC75772|ensemblbacteria:BAE76807	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:hype_ecoli(display_long)|uniprotkb:hypE(gene name)|psi-mi:hypE(display_short)|uniprotkb:Hydrogenase maturation factor HypE(gene name synonym)|uniprotkb:JW2700(locus name)|uniprotkb:b2730(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851507	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	refseq:NP_417210.2|refseq:WP_001059922.1|dip:DIP-9999N|ensemblbacteria:AAC75772(transcript)|ensemblbacteria:b2730(gene)|ensemblbacteria:BAE76807(gene)|ensemblbacteria:BAE76807(transcript)|go:"GO:0016829"(lyase activity)|go:"GO:0046892"(peptidyl-S-carbamoyl-L-cysteine dehydration)|go:"GO:0051604"(protein maturation)|interpro:IPR010918(AIR synthase related protein, C-terminal)|interpro:IPR011854(Hydrogenase expression/formation protein HypE)|interpro:IPR016188(PurM, N-terminal-like)|interpro:IPR036676|interpro:IPR036921|rcsb pdb:2I6R	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P24192 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:wOc/noYpjxe923sUb/GJww3u77483333	rogid:ZeGMKm2MPic8eLKZhUNkxiXX80o83333	intact-crc:15AC9F7E684095B3|rigid:FiX5YdUK6EzXRBeu0MfpEdPn9EA	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P24192	uniprotkb:P0ACE0	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	intact:EBI-549849|uniprotkb:P37181|uniprotkb:Q2M9K1|ensemblbacteria:AAC76030|ensemblbacteria:BAE77055	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:mbhm_ecoli(display_long)|uniprotkb:hybC(gene name)|psi-mi:hybC(display_short)|uniprotkb:b2994(locus name)|uniprotkb:JW2962(locus name)|uniprotkb:Membrane-bound hydrogenase 2 large subunit(gene name synonym)|uniprotkb:NiFe hydrogenase(gene name synonym)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851507	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	refseq:NP_417468.1|refseq:WP_000083065.1|ensemblbacteria:AAC76030(transcript)|ensemblbacteria:b2994(gene)|ensemblbacteria:BAE77055(gene)|ensemblbacteria:BAE77055(transcript)|go:"GO:0008901"(ferredoxin hydrogenase activity)|go:"GO:0016151"(nickel cation binding)|go:"GO:0031236"(extrinsic component of periplasmic side of plasma membrane)|go:"GO:0033748"("hydrogenase (acceptor) activity")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR018194|interpro:IPR029014|rcsb pdb:6EHQ|rcsb pdb:6EHS|rcsb pdb:6EN9|rcsb pdb:6G7M|rcsb pdb:6GAM|rcsb pdb:6GAN|rcsb pdb:6SYO|dip:DIP-36022N	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P24192 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:wOc/noYpjxe923sUb/GJww3u77483333	rogid:VPubvzOzdVl1a7bxWwnLl5p+kJY83333	intact-crc:15AC9F7E684095B3|rigid:FiX5YdUK6EzXRBeu0MfpEdPn9EA	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P24192	uniprotkb:Q6Q099	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	intact:EBI-851511|ensemblbacteria:PPI91468|ensemblbacteria:PPI96359	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:q6q099_ecolx(display_long)|uniprotkb:mopA(gene name)|psi-mi:mopA(display_short)|uniprotkb:GroEL protein(gene name synonym)|uniprotkb:Protein Cpn60(gene name synonym)|uniprotkb:groEL(gene name synonym)|uniprotkb:groL(gene name synonym)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:562(ecolx)|taxid:562(Escherichia coli)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851507	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	interpro:IPR018370|interpro:IPR001844(Chaperonin Cpn60)|interpro:IPR002423(Chaperonin Cpn60/TCP-1)|refseq:WP_000729122.1|interpro:IPR027410|interpro:IPR027409|interpro:IPR027413|ensemblbacteria:C4J69_05785(gene)|ensemblbacteria:C4J69_17890(gene)|ensemblbacteria:PPI91468(transcript)|ensemblbacteria:PPI96359(transcript)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0042026"(protein refolding)|go:"GO:0051082"(unfolded protein binding)	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P24192 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:wOc/noYpjxe923sUb/GJww3u77483333	rogid:217IgzYntbNra8AVRZRxdsYfer0562	intact-crc:15AC9F7E684095B3|rigid:FiX5YdUK6EzXRBeu0MfpEdPn9EA	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P24192	uniprotkb:P0AAM3	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	intact:EBI-552654|uniprotkb:P24191|uniprotkb:Q2MAA1|ensemblbacteria:AAC75770|ensemblbacteria:BAE76805	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:hypc_ecoli(display_long)|uniprotkb:hypC(gene name)|psi-mi:hypC(display_short)|uniprotkb:Chaperone-type protein HypC(gene name synonym)|uniprotkb:Hydrogenase accessory chaperone HypC(gene name synonym)|uniprotkb:JW2698(locus name)|uniprotkb:b2728(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851507	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	refseq:NP_417208.1|refseq:WP_000334881.1|dip:DIP-36430N|ensemblbacteria:AAC75770(transcript)|ensemblbacteria:b2728(gene)|ensemblbacteria:BAE76805(gene)|ensemblbacteria:BAE76805(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|rcsb pdb:2OT2	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P24192 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accessory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:wOc/noYpjxe923sUb/GJww3u77483333	rogid:cwpPKB7YGjp2PwllkXY3igEuBx883333	intact-crc:15AC9F7E684095B3|rigid:FiX5YdUK6EzXRBeu0MfpEdPn9EA	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM3	uniprotkb:P77329	intact:EBI-552654|uniprotkb:P24191|uniprotkb:Q2MAA1|ensemblbacteria:AAC75770|ensemblbacteria:BAE76805	intact:EBI-548413|uniprotkb:P76974|ensemblbacteria:AAC75540|ensemblbacteria:BAA16375	psi-mi:hypc_ecoli(display_long)|uniprotkb:hypC(gene name)|psi-mi:hypC(display_short)|uniprotkb:Chaperone-type protein HypC(gene name synonym)|uniprotkb:Hydrogenase accessory chaperone HypC(gene name synonym)|uniprotkb:JW2698(locus name)|uniprotkb:b2728(locus name)	psi-mi:hyfg_ecoli(display_long)|uniprotkb:hyfG(gene name)|psi-mi:hyfG(display_short)|uniprotkb:b2487(locus name)|uniprotkb:JW2472(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851527	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417208.1|refseq:WP_000334881.1|dip:DIP-36430N|ensemblbacteria:AAC75770(transcript)|ensemblbacteria:b2728(gene)|ensemblbacteria:BAE76805(gene)|ensemblbacteria:BAE76805(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|rcsb pdb:2OT2	refseq:NP_416982.1|refseq:WP_001102321.1|dip:DIP-9989N|ensemblbacteria:AAC75540(transcript)|ensemblbacteria:b2487(gene)|ensemblbacteria:BAA16375(gene)|ensemblbacteria:BAA16375(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051540"(metal cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014|interpro:IPR037232	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P0AAM3 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accesory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:cwpPKB7YGjp2PwllkXY3igEuBx883333	rogid:4vTpUxG0L11XkQMC4arqECyfVA083333	intact-crc:68E5A4F7C446540D|rigid:5VYZ+w5YTyf5RDyXRJFC7J8KVXU	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM3	uniprotkb:P24193	intact:EBI-552654|uniprotkb:P24191|uniprotkb:Q2MAA1|ensemblbacteria:AAC75770|ensemblbacteria:BAE76805	intact:EBI-552743|uniprotkb:Q2MA99|uniprotkb:Q46886|ensemblbacteria:AAC75772|ensemblbacteria:BAE76807	psi-mi:hypc_ecoli(display_long)|uniprotkb:hypC(gene name)|psi-mi:hypC(display_short)|uniprotkb:Chaperone-type protein HypC(gene name synonym)|uniprotkb:Hydrogenase accessory chaperone HypC(gene name synonym)|uniprotkb:JW2698(locus name)|uniprotkb:b2728(locus name)	psi-mi:hype_ecoli(display_long)|uniprotkb:hypE(gene name)|psi-mi:hypE(display_short)|uniprotkb:Hydrogenase maturation factor HypE(gene name synonym)|uniprotkb:JW2700(locus name)|uniprotkb:b2730(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851527	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417208.1|refseq:WP_000334881.1|dip:DIP-36430N|ensemblbacteria:AAC75770(transcript)|ensemblbacteria:b2728(gene)|ensemblbacteria:BAE76805(gene)|ensemblbacteria:BAE76805(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|rcsb pdb:2OT2	refseq:NP_417210.2|refseq:WP_001059922.1|dip:DIP-9999N|ensemblbacteria:AAC75772(transcript)|ensemblbacteria:b2730(gene)|ensemblbacteria:BAE76807(gene)|ensemblbacteria:BAE76807(transcript)|go:"GO:0016829"(lyase activity)|go:"GO:0046892"(peptidyl-S-carbamoyl-L-cysteine dehydration)|go:"GO:0051604"(protein maturation)|interpro:IPR010918(AIR synthase related protein, C-terminal)|interpro:IPR011854(Hydrogenase expression/formation protein HypE)|interpro:IPR016188(PurM, N-terminal-like)|interpro:IPR036676|interpro:IPR036921|rcsb pdb:2I6R	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P0AAM3 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accesory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:cwpPKB7YGjp2PwllkXY3igEuBx883333	rogid:ZeGMKm2MPic8eLKZhUNkxiXX80o83333	intact-crc:68E5A4F7C446540D|rigid:5VYZ+w5YTyf5RDyXRJFC7J8KVXU	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM3	uniprotkb:P16431	intact:EBI-552654|uniprotkb:P24191|uniprotkb:Q2MAA1|ensemblbacteria:AAC75770|ensemblbacteria:BAE76805	intact:EBI-552702|uniprotkb:Q2MAA8|ensemblbacteria:AAC75763|ensemblbacteria:BAE76798	psi-mi:hypc_ecoli(display_long)|uniprotkb:hypC(gene name)|psi-mi:hypC(display_short)|uniprotkb:Chaperone-type protein HypC(gene name synonym)|uniprotkb:Hydrogenase accessory chaperone HypC(gene name synonym)|uniprotkb:JW2698(locus name)|uniprotkb:b2728(locus name)	psi-mi:hyce_ecoli(display_long)|uniprotkb:Hydrogenase-3 component E(gene name synonym)|uniprotkb:hycE(gene name)|psi-mi:hycE(display_short)|uniprotkb:hevE(gene name synonym)|uniprotkb:b2721(locus name)|uniprotkb:JW2691(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851527	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417208.1|refseq:WP_000334881.1|dip:DIP-36430N|ensemblbacteria:AAC75770(transcript)|ensemblbacteria:b2728(gene)|ensemblbacteria:BAE76805(gene)|ensemblbacteria:BAE76805(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|rcsb pdb:2OT2	refseq:NP_417201.1|refseq:WP_001288122.1|dip:DIP-9975N|interpro:IPR037232|ensemblbacteria:AAC75763(transcript)|ensemblbacteria:b2721(gene)|ensemblbacteria:BAE76798(gene)|ensemblbacteria:BAE76798(transcript)|go:"GO:0008137"("NADH dehydrogenase (ubiquinone) activity")|go:"GO:0016151"(nickel cation binding)|go:"GO:0048038"(quinone binding)|go:"GO:0051287"(NAD binding)|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|interpro:IPR001135(NADH-ubiquinone oxidoreductase, chain 49kDa)|interpro:IPR001268("NADH dehydrogenase (ubiquinone), 30 kDa subunit")|interpro:IPR001501(Nickel-dependent hydrogenase, large subunit)|interpro:IPR014029(NADH-ubiquinone oxidoreductase, chain 49kDa, conserved site)|interpro:IPR029014	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P0AAM3 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accesory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:cwpPKB7YGjp2PwllkXY3igEuBx883333	rogid:4sPl0IkMrOsHgBgXMcL2vxInUTc83333	intact-crc:68E5A4F7C446540D|rigid:5VYZ+w5YTyf5RDyXRJFC7J8KVXU	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)
uniprotkb:P0AAM3	uniprotkb:P24192	intact:EBI-552654|uniprotkb:P24191|uniprotkb:Q2MAA1|ensemblbacteria:AAC75770|ensemblbacteria:BAE76805	intact:EBI-552711|uniprotkb:Q2MAA0|uniprotkb:Q46885|ensemblbacteria:AAC75771|ensemblbacteria:BAE76806	psi-mi:hypc_ecoli(display_long)|uniprotkb:hypC(gene name)|psi-mi:hypC(display_short)|uniprotkb:Chaperone-type protein HypC(gene name synonym)|uniprotkb:Hydrogenase accessory chaperone HypC(gene name synonym)|uniprotkb:JW2698(locus name)|uniprotkb:b2728(locus name)	psi-mi:hypd_ecoli(display_long)|uniprotkb:hypD(gene name)|psi-mi:hypD(display_short)|uniprotkb:Hydrogenase isoenzymes formation protein HypD(gene name synonym)|uniprotkb:JW2699(locus name)|uniprotkb:b2729(locus name)	psi-mi:"MI:0096"(pull down)	Butland et al. (2006)	pubmed:16412426	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-851527	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_417208.1|refseq:WP_000334881.1|dip:DIP-36430N|ensemblbacteria:AAC75770(transcript)|ensemblbacteria:b2728(gene)|ensemblbacteria:BAE76805(gene)|ensemblbacteria:BAE76805(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0051604"(protein maturation)|go:"GO:1902670"(carbon dioxide binding)|interpro:IPR001109("Hydrogenase expression/formation protein (HUPF/HYPC)")|interpro:IPR019812|rcsb pdb:2OT2	refseq:NP_417209.1|refseq:WP_001212985.1|dip:DIP-9998N|go:"GO:0051539"(4 iron, 4 sulfur cluster binding)|go:"GO:0051604"(protein maturation)|go:"GO:0070025"(carbon monoxide binding)|interpro:IPR002780(Hydrogenase formation HypD protein)|interpro:IPR042243|interpro:IPR042244|ensemblbacteria:AAC75771(transcript)|ensemblbacteria:b2729(gene)|ensemblbacteria:BAE76806(gene)|ensemblbacteria:BAE76806(transcript)|go:"GO:0005506"(iron ion binding)|go:"GO:0006464"(cellular protein modification process)	go:"GO:0046893"(iron incorporation into hydrogenase diiron subcluster via L-cysteine ligation)	-	-	expression level:P0AAM3 endogenous|comment:Biosynthesis of the Nickel- and Iron-containing catalytic center of [NiFe] hydrogenase enzymes located in the larger subunit requires the activity of multiple accesory proteins.	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2006/02/13	2014/10/16	rogid:cwpPKB7YGjp2PwllkXY3igEuBx883333	rogid:wOc/noYpjxe923sUb/GJww3u77483333	intact-crc:68E5A4F7C446540D|rigid:5VYZ+w5YTyf5RDyXRJFC7J8KVXU	false	tag:c-c	-	-	-	psi-mi:"MI:0082"(peptide massfingerprinting)	psi-mi:"MI:0082"(peptide massfingerprinting)