#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P04275	uniprotkb:Q76LX8	intact:EBI-981819|uniprotkb:Q8TCE8|uniprotkb:Q99806|ensembl:ENSP00000261405	intact:EBI-981764|uniprotkb:Q6UY16|uniprotkb:Q710F6|uniprotkb:Q711T8|uniprotkb:Q96L37|uniprotkb:Q9H0G3|ensembl:ENSP00000360997|ensembl:ENSP00000486201|uniprotkb:Q9UGQ1	psi-mi:vwf_human(display_long)|uniprotkb:VWF(gene name)|psi-mi:VWF(display_short)|uniprotkb:F8VWF(gene name synonym)	psi-mi:ats13_human(display_long)|uniprotkb:von Willebrand factor-cleaving protease(gene name synonym)|uniprotkb:ADAMTS13(gene name)|psi-mi:ADAMTS13(display_short)|uniprotkb:C9orf8(gene name synonym)|uniprotkb:UNQ6102/PRO20085(orf name)	psi-mi:"MI:0435"(protease assay)	Zanardelli et al. (2006)	imex:IM-14440|pubmed:16221672	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-981807|imex:IM-14440-2	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000543.2|ensembl:ENSG00000110799(gene)|ensembl:ENST00000261405(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0007155"(cell adhesion)|go:"GO:0007596"(blood coagulation)|go:"GO:0007599"(hemostasis)|go:"GO:0009611"(response to wounding)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030168"(platelet activation)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031091"(platelet alpha granule)|go:"GO:0031093"(platelet alpha granule lumen)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0033093"(Weibel-Palade body)|go:"GO:0042802"(identical protein binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051087"(chaperone binding)|dip:DIP-29667N|reactome:R-HSA-9672391|reactome:R-HSA-9672393|rcsb pdb:5BV8|rcsb pdb:6FWN|rcsb pdb:6N29|rcsb pdb:7KWO|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-HSA-114608|reactome:R-HSA-140837|reactome:R-HSA-216083|reactome:R-HSA-354192|reactome:R-HSA-354194|reactome:R-HSA-372708|reactome:R-HSA-430116|reactome:R-HSA-5674135|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-75892|reactome:R-HSA-76009|rcsb pdb:4NT5|reactome:R-HSA-9649948|reactome:R-HSA-9656223|go:"GO:1902533"(positive regulation of intracellular signal transduction)|interpro:IPR001007(von Willebrand factor, type C)|interpro:IPR001846(von Willebrand factor, type D)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR002919(Protease inhibitor I8, cysteine-rich trypsin inhibitor-like)|interpro:IPR006207(Cystine knot, C-terminal)|interpro:IPR014853(Region of unknown function DUF1787)|interpro:IPR032361|interpro:IPR036084|interpro:IPR036465|interpro:IPR037578|mint:P04275|rcsb pdb:1AO3|rcsb pdb:1ATZ|rcsb pdb:1AUQ|rcsb pdb:1FE8|rcsb pdb:1FNS|rcsb pdb:1IJB|rcsb pdb:1IJK|rcsb pdb:1M10|rcsb pdb:1OAK|rcsb pdb:1SQ0|rcsb pdb:1U0N|rcsb pdb:1UEX|rcsb pdb:2ADF|rcsb pdb:2MHP|rcsb pdb:2MHQ|rcsb pdb:3GXB|rcsb pdb:3HXO|rcsb pdb:3HXQ|rcsb pdb:3PPV|go:"GO:0070062"(extracellular exosome)|rcsb pdb:3PPW|rcsb pdb:3PPX|rcsb pdb:3PPY|rcsb pdb:3ZQK|rcsb pdb:4C29|rcsb pdb:4C2A|rcsb pdb:4C2B|rcsb pdb:4DMU	refseq:NP_620594.1|refseq:NP_620595.1|refseq:NP_620596.2|ensembl:ENSG00000160323(gene)|ensembl:ENSG00000281244(gene)|ensembl:ENST00000371929(transcript)|ensembl:ENST00000626597(transcript)|go:"GO:0004222"(metalloendopeptidase activity)|rcsb pdb:3VN4|rcsb pdb:6QIG|rcsb pdb:7B01|reactome:R-HSA-5083635|reactome:R-HSA-5173214|rcsb pdb:3GHN|go:"GO:0005509"(calcium ion binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0006508"(proteolysis)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009100"(glycoprotein metabolic process)|go:"GO:0009636"(response to toxic substance)|go:"GO:0009986"(cell surface)|go:"GO:0014075"(response to amine)|go:"GO:0016485"(protein processing)|go:"GO:0030168"(platelet activation)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035864"(response to potassium ion)|go:"GO:0043171"(peptide catabolic process)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071353"(cellular response to interleukin-4)|go:"GO:0071356"(cellular response to tumor necrosis factor)|interpro:IPR000884(Thrombospondin, type I)|interpro:IPR001590(Peptidase M12B, ADAM/reprolysin)|interpro:IPR006586(ADAM, cysteine-rich)|interpro:IPR013273(Peptidase M12B, ADAM-TS)|interpro:IPR024079|interpro:IPR035914|interpro:IPR036383|interpro:IPR041645|go:"GO:0005178"(integrin binding)|rcsb pdb:3GHM|dip:DIP-36050N	omim:193400(primary-reference)|go:"GO:0004222"(metalloendopeptidase activity)	-	-	kinetics:"kcat/Km 6.39 (plus or minus 1.18) E4 per M per s"|comment:All of the residue positions given map onto the Uniprot entry P04275 for VWF except Glu 1874 which the Uniprot entry states is a Gly.|figure legend:2A, 4A, 4C, Table 1|resulting-ptm:Cleavage product fractions were collected and analyzed by mass  spectrometry. For all substrates, peak 1 corresponded to the tagged  N-terminal cleavage product up to Tyr1605 and peak 2 to the  C-terminal fragment, in each case starting at Met1606. This confirmed that the Tyr1605-Met1606 was cleaved.|dataset:BioCreative - Critical Assessment of Information Extraction systems in Biology|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/08/03	2014/10/16	rogid:yeGxC2SyyvUZ3hNciww1o8TmY5Q9606	rogid:83MiOkL7ZADU7fCPIDcRGLJDOqs9606	intact-crc:CF8503FA7D2802C9|rigid:9pOb3esP1rl4eLhdKTZpK1Ok6Rk	false	binding-associated region:1593-1668(IPR002035)|his tag:n-n|tag:n-n	his tag:n-n|myc tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q76LX8	uniprotkb:P04275	intact:EBI-981764|uniprotkb:Q6UY16|uniprotkb:Q710F6|uniprotkb:Q711T8|uniprotkb:Q96L37|uniprotkb:Q9H0G3|ensembl:ENSP00000360997|ensembl:ENSP00000486201|uniprotkb:Q9UGQ1	intact:EBI-981819|uniprotkb:Q8TCE8|uniprotkb:Q99806|ensembl:ENSP00000261405	psi-mi:ats13_human(display_long)|uniprotkb:von Willebrand factor-cleaving protease(gene name synonym)|uniprotkb:ADAMTS13(gene name)|psi-mi:ADAMTS13(display_short)|uniprotkb:C9orf8(gene name synonym)|uniprotkb:UNQ6102/PRO20085(orf name)	psi-mi:vwf_human(display_long)|uniprotkb:VWF(gene name)|psi-mi:VWF(display_short)|uniprotkb:F8VWF(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Zanardelli et al. (2006)	imex:IM-14440|pubmed:16221672	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-982071|imex:IM-14440-3	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_620594.1|refseq:NP_620595.1|refseq:NP_620596.2|ensembl:ENSG00000160323(gene)|ensembl:ENSG00000281244(gene)|ensembl:ENST00000371929(transcript)|ensembl:ENST00000626597(transcript)|go:"GO:0004222"(metalloendopeptidase activity)|rcsb pdb:3VN4|rcsb pdb:6QIG|rcsb pdb:7B01|reactome:R-HSA-5083635|reactome:R-HSA-5173214|rcsb pdb:3GHN|go:"GO:0005509"(calcium ion binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0006508"(proteolysis)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009100"(glycoprotein metabolic process)|go:"GO:0009636"(response to toxic substance)|go:"GO:0009986"(cell surface)|go:"GO:0014075"(response to amine)|go:"GO:0016485"(protein processing)|go:"GO:0030168"(platelet activation)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035864"(response to potassium ion)|go:"GO:0043171"(peptide catabolic process)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071353"(cellular response to interleukin-4)|go:"GO:0071356"(cellular response to tumor necrosis factor)|interpro:IPR000884(Thrombospondin, type I)|interpro:IPR001590(Peptidase M12B, ADAM/reprolysin)|interpro:IPR006586(ADAM, cysteine-rich)|interpro:IPR013273(Peptidase M12B, ADAM-TS)|interpro:IPR024079|interpro:IPR035914|interpro:IPR036383|interpro:IPR041645|go:"GO:0005178"(integrin binding)|rcsb pdb:3GHM|dip:DIP-36050N	refseq:NP_000543.2|ensembl:ENSG00000110799(gene)|ensembl:ENST00000261405(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0007155"(cell adhesion)|go:"GO:0007596"(blood coagulation)|go:"GO:0007599"(hemostasis)|go:"GO:0009611"(response to wounding)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030168"(platelet activation)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031091"(platelet alpha granule)|go:"GO:0031093"(platelet alpha granule lumen)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0033093"(Weibel-Palade body)|go:"GO:0042802"(identical protein binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051087"(chaperone binding)|dip:DIP-29667N|reactome:R-HSA-9672391|reactome:R-HSA-9672393|rcsb pdb:5BV8|rcsb pdb:6FWN|rcsb pdb:6N29|rcsb pdb:7KWO|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-HSA-114608|reactome:R-HSA-140837|reactome:R-HSA-216083|reactome:R-HSA-354192|reactome:R-HSA-354194|reactome:R-HSA-372708|reactome:R-HSA-430116|reactome:R-HSA-5674135|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-75892|reactome:R-HSA-76009|rcsb pdb:4NT5|reactome:R-HSA-9649948|reactome:R-HSA-9656223|go:"GO:1902533"(positive regulation of intracellular signal transduction)|interpro:IPR001007(von Willebrand factor, type C)|interpro:IPR001846(von Willebrand factor, type D)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR002919(Protease inhibitor I8, cysteine-rich trypsin inhibitor-like)|interpro:IPR006207(Cystine knot, C-terminal)|interpro:IPR014853(Region of unknown function DUF1787)|interpro:IPR032361|interpro:IPR036084|interpro:IPR036465|interpro:IPR037578|mint:P04275|rcsb pdb:1AO3|rcsb pdb:1ATZ|rcsb pdb:1AUQ|rcsb pdb:1FE8|rcsb pdb:1FNS|rcsb pdb:1IJB|rcsb pdb:1IJK|rcsb pdb:1M10|rcsb pdb:1OAK|rcsb pdb:1SQ0|rcsb pdb:1U0N|rcsb pdb:1UEX|rcsb pdb:2ADF|rcsb pdb:2MHP|rcsb pdb:2MHQ|rcsb pdb:3GXB|rcsb pdb:3HXO|rcsb pdb:3HXQ|rcsb pdb:3PPV|go:"GO:0070062"(extracellular exosome)|rcsb pdb:3PPW|rcsb pdb:3PPX|rcsb pdb:3PPY|rcsb pdb:3ZQK|rcsb pdb:4C29|rcsb pdb:4C2A|rcsb pdb:4C2B|rcsb pdb:4DMU	omim:193400(primary-reference)|go:"GO:0004222"(metalloendopeptidase activity)	-	-	resulting-ptm:The scissile bond in VWF is predicted from a homology model to be located in the center of the folded A2 domain.|kinetics:"For the ADAMTS13-dependent proteolysis of VWF115 (n equals 4), they derived a kcat of 0.14 plus or minus 0.06 per s.  The Km for this reaction was 1.61 plus or minus 4.7 E-7 M and the specificity constant (catalytic efficiency, kcat/Km) was 8.70 E4 per M per s."|kinetics:From the gels in Fig. 4A, it seemed as if all of the mutants exhibited reduced rates of proteolysis.|comment:All of the residue positions given map onto the Uniprot entry P04275 for VWF except Glu 1874 which the Uniprot entry states is a Gly.|figure legend:2B, 3D, 4, Table 1|kinetics:"The kcat_Km (E4 per M per s) are as follows: VWF115 7.83 plus or minus 1.18, VWF115-A3 6.70 plus or minus 2.78, VWF76 6.39 plus or minus 1.18, for VWF115 variants: arg1583ala 6.19, asp1640ala, 2.74, glu1638ala 2.74, lys1617ala 1.90, asp1614ala 1.36 plus or minus 0.26, glu1615ala 1.13 plus or minus 0.30, asp1587ala 0.64 plus or minus 0.10, asp1614ala_glu1615ala_lys1617ala 0.59."|kinetics:"Mutation of Glu1638 and Glu1640 induced a mild (2�3-fold) reduction  in catalytic efficiency. The most affected (over 12-fold reduction) single mutant was D1587A, the kcat/Km of which was 0.64 plus or minus 0.10x 10E4 per M per s. The kcat/Km for K1617A, D1614A, and E1615A, which are predicted to lie on the same surface-exposed loop, were 1.90, 1.36, and 1.13 E4 per M per s, respectively. The  triple mutant DEK1614/5/7AAA containing all 3 residues substituted  for alanine was generated. Whereas individually these mutations caused a 4�7-fold reduction in kcat/Km, in combination the catalytic efficiency was over 13-fold less than wild-type VWF115, implying that the amino acids in this charged loop may act in synergy."|dataset:BioCreative - Critical Assessment of Information Extraction systems in Biology|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/08/03	2014/10/16	rogid:83MiOkL7ZADU7fCPIDcRGLJDOqs9606	rogid:yeGxC2SyyvUZ3hNciww1o8TmY5Q9606	intact-crc:3D661F801893D2E1|rigid:9pOb3esP1rl4eLhdKTZpK1Ok6Rk	false	myc tag:n-n|his tag:n-n	his tag:n-n|tag:n-n|mutation:1583-1583|mutation:1615-1615,1617-1617,1614-1614|mutation:1587-1587|mutation:1614-1614|mutation:1615-1615|mutation:1617-1617|mutation:1638-1638|mutation:1640-1640|sufficient binding region:1554-1668(IPR002035)	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q76LX8	uniprotkb:P04275	intact:EBI-981764|uniprotkb:Q6UY16|uniprotkb:Q710F6|uniprotkb:Q711T8|uniprotkb:Q96L37|uniprotkb:Q9H0G3|ensembl:ENSP00000360997|ensembl:ENSP00000486201|uniprotkb:Q9UGQ1	intact:EBI-981819|uniprotkb:Q8TCE8|uniprotkb:Q99806|ensembl:ENSP00000261405	psi-mi:ats13_human(display_long)|uniprotkb:von Willebrand factor-cleaving protease(gene name synonym)|uniprotkb:ADAMTS13(gene name)|psi-mi:ADAMTS13(display_short)|uniprotkb:C9orf8(gene name synonym)|uniprotkb:UNQ6102/PRO20085(orf name)	psi-mi:vwf_human(display_long)|uniprotkb:VWF(gene name)|psi-mi:VWF(display_short)|uniprotkb:F8VWF(gene name synonym)	psi-mi:"MI:0435"(protease assay)	Zanardelli et al. (2006)	imex:IM-14440|pubmed:16221672	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-982419|imex:IM-14440-4	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_620594.1|refseq:NP_620595.1|refseq:NP_620596.2|ensembl:ENSG00000160323(gene)|ensembl:ENSG00000281244(gene)|ensembl:ENST00000371929(transcript)|ensembl:ENST00000626597(transcript)|go:"GO:0004222"(metalloendopeptidase activity)|rcsb pdb:3VN4|rcsb pdb:6QIG|rcsb pdb:7B01|reactome:R-HSA-5083635|reactome:R-HSA-5173214|rcsb pdb:3GHN|go:"GO:0005509"(calcium ion binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0006508"(proteolysis)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009100"(glycoprotein metabolic process)|go:"GO:0009636"(response to toxic substance)|go:"GO:0009986"(cell surface)|go:"GO:0014075"(response to amine)|go:"GO:0016485"(protein processing)|go:"GO:0030168"(platelet activation)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035864"(response to potassium ion)|go:"GO:0043171"(peptide catabolic process)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071353"(cellular response to interleukin-4)|go:"GO:0071356"(cellular response to tumor necrosis factor)|interpro:IPR000884(Thrombospondin, type I)|interpro:IPR001590(Peptidase M12B, ADAM/reprolysin)|interpro:IPR006586(ADAM, cysteine-rich)|interpro:IPR013273(Peptidase M12B, ADAM-TS)|interpro:IPR024079|interpro:IPR035914|interpro:IPR036383|interpro:IPR041645|go:"GO:0005178"(integrin binding)|rcsb pdb:3GHM|dip:DIP-36050N	refseq:NP_000543.2|ensembl:ENSG00000110799(gene)|ensembl:ENST00000261405(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0007155"(cell adhesion)|go:"GO:0007596"(blood coagulation)|go:"GO:0007599"(hemostasis)|go:"GO:0009611"(response to wounding)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030168"(platelet activation)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031091"(platelet alpha granule)|go:"GO:0031093"(platelet alpha granule lumen)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0033093"(Weibel-Palade body)|go:"GO:0042802"(identical protein binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051087"(chaperone binding)|dip:DIP-29667N|reactome:R-HSA-9672391|reactome:R-HSA-9672393|rcsb pdb:5BV8|rcsb pdb:6FWN|rcsb pdb:6N29|rcsb pdb:7KWO|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-HSA-114608|reactome:R-HSA-140837|reactome:R-HSA-216083|reactome:R-HSA-354192|reactome:R-HSA-354194|reactome:R-HSA-372708|reactome:R-HSA-430116|reactome:R-HSA-5674135|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-75892|reactome:R-HSA-76009|rcsb pdb:4NT5|reactome:R-HSA-9649948|reactome:R-HSA-9656223|go:"GO:1902533"(positive regulation of intracellular signal transduction)|interpro:IPR001007(von Willebrand factor, type C)|interpro:IPR001846(von Willebrand factor, type D)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR002919(Protease inhibitor I8, cysteine-rich trypsin inhibitor-like)|interpro:IPR006207(Cystine knot, C-terminal)|interpro:IPR014853(Region of unknown function DUF1787)|interpro:IPR032361|interpro:IPR036084|interpro:IPR036465|interpro:IPR037578|mint:P04275|rcsb pdb:1AO3|rcsb pdb:1ATZ|rcsb pdb:1AUQ|rcsb pdb:1FE8|rcsb pdb:1FNS|rcsb pdb:1IJB|rcsb pdb:1IJK|rcsb pdb:1M10|rcsb pdb:1OAK|rcsb pdb:1SQ0|rcsb pdb:1U0N|rcsb pdb:1UEX|rcsb pdb:2ADF|rcsb pdb:2MHP|rcsb pdb:2MHQ|rcsb pdb:3GXB|rcsb pdb:3HXO|rcsb pdb:3HXQ|rcsb pdb:3PPV|go:"GO:0070062"(extracellular exosome)|rcsb pdb:3PPW|rcsb pdb:3PPX|rcsb pdb:3PPY|rcsb pdb:3ZQK|rcsb pdb:4C29|rcsb pdb:4C2A|rcsb pdb:4C2B|rcsb pdb:4DMU	omim:193400(primary-reference)|go:"GO:0004222"(metalloendopeptidase activity)	-	-	figure legend:5|kinetics:"When samples containing 500 nM VWFA2 were analyzed by HPLC (Fig. 5C), it was clear that the cleavage reaction did not proceed toward completion (approx. 28% after 5 h). The D1587A, D1614A, and E1615A VWFA2 mutants were proteolyzed either at a similar or slower rate (Fig. 5, A and C), suggesting that these mutations do not alter the A2 domain structure markedly. Under denaturing conditions (1 M urea), the wild-type VWFA2 was proteolyzed faster, although it still did not reach completion (approx. 50% after 5 h) (Fig. 5, B and D). Although all the mutants were cleaved at a higher rate under denaturing conditions, they were cleaved appreciably slower than VWFA2, corroborating the direct influence of these mutations upon VWF115 proteolysis."|comment:All of the residue positions given map onto the Uniprot entry P04275 for VWF except Glu 1874 which the Uniprot entry states is a Gly.|dataset:BioCreative - Critical Assessment of Information Extraction systems in Biology|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/08/03	2014/10/16	rogid:83MiOkL7ZADU7fCPIDcRGLJDOqs9606	rogid:yeGxC2SyyvUZ3hNciww1o8TmY5Q9606	intact-crc:A03E16033E2587F5|rigid:9pOb3esP1rl4eLhdKTZpK1Ok6Rk	false	his tag:n-n|myc tag:n-n	his tag:n-n|tag:n-n|sufficient binding region:1473-1668(IPR002035)|mutation:1614-1614|mutation:1615-1615|mutation:1587-1587	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P04275	uniprotkb:Q76LX8	intact:EBI-981819|uniprotkb:Q8TCE8|uniprotkb:Q99806|ensembl:ENSP00000261405	intact:EBI-981764|uniprotkb:Q6UY16|uniprotkb:Q710F6|uniprotkb:Q711T8|uniprotkb:Q96L37|uniprotkb:Q9H0G3|ensembl:ENSP00000360997|ensembl:ENSP00000486201|uniprotkb:Q9UGQ1	psi-mi:vwf_human(display_long)|uniprotkb:VWF(gene name)|psi-mi:VWF(display_short)|uniprotkb:F8VWF(gene name synonym)	psi-mi:ats13_human(display_long)|uniprotkb:von Willebrand factor-cleaving protease(gene name synonym)|uniprotkb:ADAMTS13(gene name)|psi-mi:ADAMTS13(display_short)|uniprotkb:C9orf8(gene name synonym)|uniprotkb:UNQ6102/PRO20085(orf name)	psi-mi:"MI:0435"(protease assay)	Zanardelli et al. (2006)	imex:IM-14440|pubmed:16221672	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-1007861|imex:IM-14440-1	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000543.2|ensembl:ENSG00000110799(gene)|ensembl:ENST00000261405(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0007155"(cell adhesion)|go:"GO:0007596"(blood coagulation)|go:"GO:0007599"(hemostasis)|go:"GO:0009611"(response to wounding)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030168"(platelet activation)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031091"(platelet alpha granule)|go:"GO:0031093"(platelet alpha granule lumen)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0033093"(Weibel-Palade body)|go:"GO:0042802"(identical protein binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051087"(chaperone binding)|dip:DIP-29667N|reactome:R-HSA-9672391|reactome:R-HSA-9672393|rcsb pdb:5BV8|rcsb pdb:6FWN|rcsb pdb:6N29|rcsb pdb:7KWO|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-HSA-114608|reactome:R-HSA-140837|reactome:R-HSA-216083|reactome:R-HSA-354192|reactome:R-HSA-354194|reactome:R-HSA-372708|reactome:R-HSA-430116|reactome:R-HSA-5674135|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-75892|reactome:R-HSA-76009|rcsb pdb:4NT5|reactome:R-HSA-9649948|reactome:R-HSA-9656223|go:"GO:1902533"(positive regulation of intracellular signal transduction)|interpro:IPR001007(von Willebrand factor, type C)|interpro:IPR001846(von Willebrand factor, type D)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR002919(Protease inhibitor I8, cysteine-rich trypsin inhibitor-like)|interpro:IPR006207(Cystine knot, C-terminal)|interpro:IPR014853(Region of unknown function DUF1787)|interpro:IPR032361|interpro:IPR036084|interpro:IPR036465|interpro:IPR037578|mint:P04275|rcsb pdb:1AO3|rcsb pdb:1ATZ|rcsb pdb:1AUQ|rcsb pdb:1FE8|rcsb pdb:1FNS|rcsb pdb:1IJB|rcsb pdb:1IJK|rcsb pdb:1M10|rcsb pdb:1OAK|rcsb pdb:1SQ0|rcsb pdb:1U0N|rcsb pdb:1UEX|rcsb pdb:2ADF|rcsb pdb:2MHP|rcsb pdb:2MHQ|rcsb pdb:3GXB|rcsb pdb:3HXO|rcsb pdb:3HXQ|rcsb pdb:3PPV|go:"GO:0070062"(extracellular exosome)|rcsb pdb:3PPW|rcsb pdb:3PPX|rcsb pdb:3PPY|rcsb pdb:3ZQK|rcsb pdb:4C29|rcsb pdb:4C2A|rcsb pdb:4C2B|rcsb pdb:4DMU	refseq:NP_620594.1|refseq:NP_620595.1|refseq:NP_620596.2|ensembl:ENSG00000160323(gene)|ensembl:ENSG00000281244(gene)|ensembl:ENST00000371929(transcript)|ensembl:ENST00000626597(transcript)|go:"GO:0004222"(metalloendopeptidase activity)|rcsb pdb:3VN4|rcsb pdb:6QIG|rcsb pdb:7B01|reactome:R-HSA-5083635|reactome:R-HSA-5173214|rcsb pdb:3GHN|go:"GO:0005509"(calcium ion binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0006508"(proteolysis)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009100"(glycoprotein metabolic process)|go:"GO:0009636"(response to toxic substance)|go:"GO:0009986"(cell surface)|go:"GO:0014075"(response to amine)|go:"GO:0016485"(protein processing)|go:"GO:0030168"(platelet activation)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035864"(response to potassium ion)|go:"GO:0043171"(peptide catabolic process)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071353"(cellular response to interleukin-4)|go:"GO:0071356"(cellular response to tumor necrosis factor)|interpro:IPR000884(Thrombospondin, type I)|interpro:IPR001590(Peptidase M12B, ADAM/reprolysin)|interpro:IPR006586(ADAM, cysteine-rich)|interpro:IPR013273(Peptidase M12B, ADAM-TS)|interpro:IPR024079|interpro:IPR035914|interpro:IPR036383|interpro:IPR041645|go:"GO:0005178"(integrin binding)|rcsb pdb:3GHM|dip:DIP-36050N	omim:193400(primary-reference)|go:"GO:0004222"(metalloendopeptidase activity)	-	-	kinetics:"kcat/Km 6.70 (plus or minus 2.78) E4 per M per s"|comment:All of the residue positions given map onto the Uniprot entry P04275 for VWF except Glu 1874 which the Uniprot entry states is a Gly.|figure legend:2 C, 4A and C, Table 1|resulting-ptm:Cleavage product fractions were collected and analyzed by mass  spectrometry. For all substrates, peak 1 corresponded to the tagged  N-terminal cleavage product up to Tyr1605 and peak 2 to the  C-terminal fragment, in each case starting at Met1606. This confirmed that the Tyr1605-Met1606 was cleaved.|dataset:BioCreative - Critical Assessment of Information Extraction systems in Biology|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/08/03	2014/10/16	rogid:yeGxC2SyyvUZ3hNciww1o8TmY5Q9606	rogid:83MiOkL7ZADU7fCPIDcRGLJDOqs9606	intact-crc:857D80694CE584D0|rigid:9pOb3esP1rl4eLhdKTZpK1Ok6Rk	false	his tag:n-n|tag:n-n|sufficient binding region:1554-1874	his tag:n-n|myc tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P04275	uniprotkb:Q76LX8	intact:EBI-981819|uniprotkb:Q8TCE8|uniprotkb:Q99806|ensembl:ENSP00000261405	intact:EBI-981764|uniprotkb:Q6UY16|uniprotkb:Q710F6|uniprotkb:Q711T8|uniprotkb:Q96L37|uniprotkb:Q9H0G3|ensembl:ENSP00000360997|ensembl:ENSP00000486201|uniprotkb:Q9UGQ1	psi-mi:vwf_human(display_long)|uniprotkb:VWF(gene name)|psi-mi:VWF(display_short)|uniprotkb:F8VWF(gene name synonym)	psi-mi:ats13_human(display_long)|uniprotkb:von Willebrand factor-cleaving protease(gene name synonym)|uniprotkb:ADAMTS13(gene name)|psi-mi:ADAMTS13(display_short)|uniprotkb:C9orf8(gene name synonym)|uniprotkb:UNQ6102/PRO20085(orf name)	psi-mi:"MI:0107"(surface plasmon resonance)	Zanardelli et al. (2006)	imex:IM-14440|pubmed:16221672	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-982617|imex:IM-14440-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_000543.2|ensembl:ENSG00000110799(gene)|ensembl:ENST00000261405(transcript)|go:"GO:0002020"(protease binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005518"(collagen binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0007155"(cell adhesion)|go:"GO:0007596"(blood coagulation)|go:"GO:0007599"(hemostasis)|go:"GO:0009611"(response to wounding)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030168"(platelet activation)|go:"GO:0031012"(extracellular matrix)|go:"GO:0031091"(platelet alpha granule)|go:"GO:0031093"(platelet alpha granule lumen)|go:"GO:0031589"(cell-substrate adhesion)|go:"GO:0033093"(Weibel-Palade body)|go:"GO:0042802"(identical protein binding)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0051087"(chaperone binding)|dip:DIP-29667N|reactome:R-HSA-9672391|reactome:R-HSA-9672393|rcsb pdb:5BV8|rcsb pdb:6FWN|rcsb pdb:6N29|rcsb pdb:7KWO|go:"GO:0062023"(collagen-containing extracellular matrix)|reactome:R-HSA-114608|reactome:R-HSA-140837|reactome:R-HSA-216083|reactome:R-HSA-354192|reactome:R-HSA-354194|reactome:R-HSA-372708|reactome:R-HSA-430116|reactome:R-HSA-5674135|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-75892|reactome:R-HSA-76009|rcsb pdb:4NT5|reactome:R-HSA-9649948|reactome:R-HSA-9656223|go:"GO:1902533"(positive regulation of intracellular signal transduction)|interpro:IPR001007(von Willebrand factor, type C)|interpro:IPR001846(von Willebrand factor, type D)|interpro:IPR002035(von Willebrand factor, type A)|interpro:IPR002919(Protease inhibitor I8, cysteine-rich trypsin inhibitor-like)|interpro:IPR006207(Cystine knot, C-terminal)|interpro:IPR014853(Region of unknown function DUF1787)|interpro:IPR032361|interpro:IPR036084|interpro:IPR036465|interpro:IPR037578|mint:P04275|rcsb pdb:1AO3|rcsb pdb:1ATZ|rcsb pdb:1AUQ|rcsb pdb:1FE8|rcsb pdb:1FNS|rcsb pdb:1IJB|rcsb pdb:1IJK|rcsb pdb:1M10|rcsb pdb:1OAK|rcsb pdb:1SQ0|rcsb pdb:1U0N|rcsb pdb:1UEX|rcsb pdb:2ADF|rcsb pdb:2MHP|rcsb pdb:2MHQ|rcsb pdb:3GXB|rcsb pdb:3HXO|rcsb pdb:3HXQ|rcsb pdb:3PPV|go:"GO:0070062"(extracellular exosome)|rcsb pdb:3PPW|rcsb pdb:3PPX|rcsb pdb:3PPY|rcsb pdb:3ZQK|rcsb pdb:4C29|rcsb pdb:4C2A|rcsb pdb:4C2B|rcsb pdb:4DMU	refseq:NP_620594.1|refseq:NP_620595.1|refseq:NP_620596.2|ensembl:ENSG00000160323(gene)|ensembl:ENSG00000281244(gene)|ensembl:ENST00000371929(transcript)|ensembl:ENST00000626597(transcript)|go:"GO:0004222"(metalloendopeptidase activity)|rcsb pdb:3VN4|rcsb pdb:6QIG|rcsb pdb:7B01|reactome:R-HSA-5083635|reactome:R-HSA-5173214|rcsb pdb:3GHN|go:"GO:0005509"(calcium ion binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005788"(endoplasmic reticulum lumen)|go:"GO:0006508"(proteolysis)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009100"(glycoprotein metabolic process)|go:"GO:0009636"(response to toxic substance)|go:"GO:0009986"(cell surface)|go:"GO:0014075"(response to amine)|go:"GO:0016485"(protein processing)|go:"GO:0030168"(platelet activation)|go:"GO:0030198"(extracellular matrix organization)|go:"GO:0031012"(extracellular matrix)|go:"GO:0035864"(response to potassium ion)|go:"GO:0043171"(peptide catabolic process)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071346"(cellular response to interferon-gamma)|go:"GO:0071353"(cellular response to interleukin-4)|go:"GO:0071356"(cellular response to tumor necrosis factor)|interpro:IPR000884(Thrombospondin, type I)|interpro:IPR001590(Peptidase M12B, ADAM/reprolysin)|interpro:IPR006586(ADAM, cysteine-rich)|interpro:IPR013273(Peptidase M12B, ADAM-TS)|interpro:IPR024079|interpro:IPR035914|interpro:IPR036383|interpro:IPR041645|go:"GO:0005178"(integrin binding)|rcsb pdb:3GHM|dip:DIP-36050N	omim:193400(primary-reference)|go:"GO:0004222"(metalloendopeptidase activity)	-	-	comment:All of the residue positions given map onto the Uniprot entry P04275 for VWF except Glu 1874 which the Uniprot entry states is a Gly.|figure legend:6|kinetics:The KD was 2.0 E-8 M for VWF115. The mutants has a reduced affinity. The estimate for the KD for the triple mutant was approx. 1.3 E-6 M but due to low binding for mutants KD values could not be derived.|comment:The results for the experiment using ADAMTS13 as the bait were not shown but the authors state that the results were similar.|dataset:BioCreative - Critical Assessment of Information Extraction systems in Biology|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2006/08/03	2019/04/02	rogid:yeGxC2SyyvUZ3hNciww1o8TmY5Q9606	rogid:83MiOkL7ZADU7fCPIDcRGLJDOqs9606	intact-crc:744C23352E0436E2|rigid:9pOb3esP1rl4eLhdKTZpK1Ok6Rk	false	tag:n-n|binding-associated region:1554-1668(IPR002035)|his tag:n-n|mutation decreasing interaction:1587-1587|mutation decreasing interaction:1614-1614|mutation decreasing interaction:1615-1615|mutation decreasing interaction:1614-1614,1615-1615,1617-1617	myc tag:n-n|his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)