#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P01120 uniprotkb:P46961 intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C intact:EBI-7809|uniprotkb:P48014|uniprotkb:D6W3U0|ensemblfungi:YPL076W psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name) psi-mi:gpi2_yeast(display_long)|uniprotkb:GPI2(gene name)|psi-mi:GPI2(display_short)|uniprotkb:GCR4(gene name synonym)|uniprotkb:YPL076W(locus name)|uniprotkb:LPF9W(orf name) psi-mi:"MI:0096"(pull down) Sobering et al. (2004) pubmed:15163411|imex:IM-19644 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-975266|imex:IM-19644-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120 refseq:NP_015249.1|sgd:S000005997|dip:DIP-4023N|ensemblfungi:YPL076W(gene)|ensemblfungi:YPL076W_mRNA(transcript)|go:"GO:0000506"("glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex")|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006506"(GPI anchor biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017176"(phosphatidylinositol N-acetylglucosaminyltransferase activity)|interpro:IPR009450(Phosphatidylinositol N-acetylglucosaminyltransferase) go:"GO:0000506"("glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex")|go:"GO:0006506"(GPI anchor biosynthetic process)|go:"GO:0003924"(GTPase activity)|go:"GO:0007265"(Ras protein signal transduction) crc64:6CAAAC531EEAE92F crc64:2BFEE04E913918DB figure legend:5B|comment:To make an efficient interaction, RAS2 has to be bound to GTP and needs a wild type effector binding loop.|expression level:Both bait and prey were overexpressed.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2006/07/24 2014/10/16 rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292 rogid:Gsrb11RW7NTnReJRUXpSWTbpl/c559292 intact-crc:783A0F69DD63DF90|rigid:rMktg4DDSDBNj7YCZixGE5Mk4z0 false glutathione s tranferase tag:?-? flag tag:n-n - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot) uniprotkb:P62651 uniprotkb:P46961 intact:EBI-975247|uniprotkb:D6W3S0|ensemblfungi:YPL096C-A intact:EBI-7809|uniprotkb:P48014|uniprotkb:D6W3U0|ensemblfungi:YPL076W psi-mi:eri1_yeast(display_long)|uniprotkb:Endoplasmic reticulum-associated Ras inhibitor protein 1(gene name synonym)|uniprotkb:ERI1(gene name)|psi-mi:ERI1(display_short)|uniprotkb:RIN1(gene name synonym)|uniprotkb:YPL096C-A(locus name) psi-mi:gpi2_yeast(display_long)|uniprotkb:GPI2(gene name)|psi-mi:GPI2(display_short)|uniprotkb:GCR4(gene name synonym)|uniprotkb:YPL076W(locus name)|uniprotkb:LPF9W(orf name) psi-mi:"MI:0007"(anti tag coimmunoprecipitation) Sobering et al. (2004) pubmed:15163411|imex:IM-19644 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0486"(UniProt) intact:EBI-975240|imex:IM-19644-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensemblfungi:YPL096C-A(gene)|ensemblfungi:YPL096C-A_mRNA(transcript)|go:"GO:0000506"("glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex")|go:"GO:0005095"(GTPase inhibitor activity)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0006506"(GPI anchor biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0007265"(Ras protein signal transduction)|sgd:S000028423|refseq:NP_690846.1 refseq:NP_015249.1|sgd:S000005997|dip:DIP-4023N|ensemblfungi:YPL076W(gene)|ensemblfungi:YPL076W_mRNA(transcript)|go:"GO:0000506"("glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex")|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006506"(GPI anchor biosynthetic process)|go:"GO:0016021"(integral component of membrane)|go:"GO:0017176"(phosphatidylinositol N-acetylglucosaminyltransferase activity)|interpro:IPR009450(Phosphatidylinositol N-acetylglucosaminyltransferase) go:"GO:0006506"(GPI anchor biosynthetic process)|go:"GO:0000506"("glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex") - crc64:2BFEE04E913918DB figure legend:5A|expression level:P46961, P62651 overexpressed.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)") - 2006/07/24 2014/10/16 rogid:uNn1QjusOBeC5dGhSLPOueMcHlw559292 rogid:Gsrb11RW7NTnReJRUXpSWTbpl/c559292 intact-crc:65DCEE061DF5E0D9|rigid:p8/Rha/lX4dwCLhsGUwHZ+irCP8 false ha tag:?-? flag tag:n-n - - psi-mi:"MI:0113"(western blot) psi-mi:"MI:0113"(western blot)