DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Structure (London, England : 1993) 2005 imex curation Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132880X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Structure (London, England : 1993) 2005 imex curation Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132878X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Structure (London, England : 1993) 2005 imex curation Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132879X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. ncap_ibvb Nucleoprotein N Nucleocapsid protein 6 protein protein ibvb ibvb IBV MASGKAAGKTDAPAPVIKLGGPKPPKVGSSGNASWFQAIKAKKLNTPPPKFEGSGVPDNENIKPSQQHGYWRRQARFKPGKGGRKPVPDAWYFYYTGTGPAADLNWGDTQDGIVWVAAKGADTKSRSNQGTRDPDKFDQYPLRFSDGGPDGNFRWDFIPLNRGRSGRSTAASSAAASRAPSREGSRGRRSDSGDDLIARAAKIIQDQQKKGSRITKAKADEMAHRRYCKRTIPPNYRVDQVFGPRTKGKEGNFGDDKMNEEGIKDGRVTAMLNLVPSSHACLFGSRVTPKLQLDGLHLRFEFTTVVPCDDPQFDNYVKICDQCVDGVGTRPKDDEPKPKSRSSSRPATRGNSPAPRQQRPKKEKKLKKQDDEADKALTSDEERNNAQLEFYDEPKVINWGDAALGENEL 562F39C475DB8872 ibvn-1 2 4 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain C-terminal Region 218-329 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ibvn-2 3 4 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain C-terminal Region 218-329 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ibvn-3 1 4 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Virology 2009 imex curation Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Cross-linking agent: Glutaraldehyde (CHEBI:64276) dxliu@imcb.a-star.edu.sg Accepted 2020-JUN-19 AT 11:07 BST AT 11:07 BST by PPORRAS Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. PloS one 2007 imex curation Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus IBV-infected Vero cells dxliu@imcb.a-star.edu.sg Accepted 2020-APR-06 AT 14:03 BST AT 14:03 BST by MGT PloS one 2007 Only protein-protein interactions imex curation imex curation Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Virology 2009 imex curation Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-JUN-19 AT 11:07 BST AT 11:07 BST by PPORRAS Virology 2009 Only protein-protein interactions imex curation imex curation Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Virology 2009 imex curation Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Only protein-protein interactions imex curation in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins With the help of https://www.bioinformatics.org/sms/rev_comp.html tool, the reverse-complement 3'UTR sequence was generated. dxliu@imcb.a-star.edu.sg Accepted 2020-JUN-19 AT 11:07 BST AT 11:07 BST by PPORRAS Virology 2009 Only protein-protein interactions imex curation imex curation Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Formation of stable homodimer via the C-terminal alpha-helical domain of coronavirus nonstructural protein 9 is critical for its function in viral replication. Virology 2009 imex curation Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-JUN-19 AT 11:07 BST AT 11:07 BST by PPORRAS Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. PloS one 2007 imex curation Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Accepted 2020-APR-06 AT 14:03 BST AT 14:03 BST by MGT PloS one 2007 Only protein-protein interactions imex curation imex curation Binding of the 5'-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription. Binding of the 5'-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription. Nucleic acids research 2012 imex curation Tan YW., Hong W., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@ntu.edu.sg Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tan YW., Hong W., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@ntu.edu.sg Accepted 2020-JUN-18 AT 08:28 BST AT 08:28 BST by KALPANAP Nucleic acids research 2012 Only protein-protein interactions imex curation imex curation Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. Coronavirus spike protein inhibits host cell translation by interaction with eIF3f. PloS one 2007 imex curation Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg Accepted 2020-APR-06 AT 14:03 BST AT 14:03 BST by MGT PloS one 2007 Only protein-protein interactions imex curation imex curation aibv_rna_antisense_3'utr Avian infectious bronchitis virus-ssrna-antisense-3'UTR rna ribonucleic acid RNA rna aibv Avian infectious bronchitis virus-Beaudette strain TGCTCTAACTCTATACTAGCCTATAAATTTTAACTATACTTAGCCAATTAAACTTAACTTAAACTAAAATTTAGCTCTTCCCCTAATGGGCGTCCTAGTGCTGTACCCTCGATCGTACTCCGCGTGGCCCCGGCACTGGCATCTTTATACCTACTCTAAACTAACTTAAACTAAACTAAAAATTAAACTAAATTAAAATCTAAGGGTCTACCGTTCGTTTCCAGGCTACTAAGTAGACAGATTAGACATTTCCCTGGCGATAGACATGTAGGTAATCAAGCTACATGCCTATCTTCCTTAACTCTTACTATTGAATTTCTACGATAAAGCAAACTAATGAGAATCACAACAATAAAAAGCACAGCAACAATACAATAAGCAAAAAGCAACCAAAAGAAGCTTAAGTATTGAACATAATCACAATCAATGCCTGTAATAATTGATAGGAAAGCACAGAAATAGTGTTTAACAAAATGTACCAGGAAACAACAGGTCCATTATGTTACTCA p0c6v3-pro_0000338322 Non-structural protein 9 ORF1a polyprotein 1a protein protein ibvb ibvb IBV NNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGVKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQ 3673 3783 2C408117DF15625B eif3f_chlae_protein EIF3F Green Monkey protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to O00303 aibv_rna_3'utr Avian infectious bronchitis virus-ssrna-3'UTR rna ribonucleic acid RNA rna aibv Avian infectious bronchitis virus-Beaudette strain TGAGTAACATAATGGACCTGTTGTTTCCTGGTACATTTTGTTAAACACTATTTCTGTGCTTTCCTATCAATTATTACAGGCATTGATTGTGATTATGTTCAATACTTAAGCTTCTTTTGGTTGCTTTTTGCTTATTGTATTGTTGCTGTGCTTTTTATTGTTGTGATTCTCATTAGTTTGCTTTATCGTAGAAATTCAATAGTAAGAGTTAAGGAAGATAGGCATGTAGCTTGATTACCTACATGTCTATCGCCAGGGAAATGTCTAATCTGTCTACTTAGTAGCCTGGAAACGAACGGTAGACCCTTAGATTTTAATTTAGTTTAATTTTTAGTTTAGTTTAAGTTAGTTTAGAGTAGGTATAAAGATGCCAGTGCCGGGGCCACGCGGAGTACGATCGAGGGTACAGCACTAGGACGCCCATTAGGGGAAGAGCTAAATTTTAGTTTAAGTTAAGTTTAATTGGCTAAGTATAGTTAAAATTTATAGGCTAGTATAGAGTTAGAGCA zcrb1_human Zinc finger CCHC-type and RNA-binding motif-containing protein 1 ZCRB1 U11/U12 small nuclear ribonucleoprotein 31 kDa protein protein protein human Homo sapiens Human MSGGLAPSKSTVYVSNLPFSLTNNDLYRIFSKYGKVVKVTIMKDKDTRKSKGVAFILFLDKDSAQNCTRAINNKQLFGRVIKASIAIDNGRAAEFIRRRNYFDKSKCYECGESGHLSYACPKNMLGEREPPKKKEKKKKKKAPEPEEEIEEVEESEDEGEDPALDSLSQAIAFQQAKIEEEQKKWKPSSGVPSTSDDSRRPRIKKSTYFSDEEELSD CC0C2BB6D2E42444 eif3f_human Eukaryotic translation initiation factor 3 subunit F Eukaryotic translation initiation factor 3 subunit 5 eIF-3-epsilon eIF3 p47 EIF3F EIF3S5 Deubiquitinating enzyme eIF3f protein protein human Homo sapiens Human MATPAVPVSAPPATPTPVPAAAPASVPAPTPAPAAAPVPAAAPASSSDPAAAAAATAAPGQTPASAQAPAQTPAPALPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL DIP protein O00303 original sequence version: 142 DIP protein O00303 original sequence version: 80 DIP protein O00303 original sequence version: 95 DIP protein O00303 original sequence version: 126 8A70FC6E2BF07737 spike_ibvb Spike glycoprotein S E2 Peplomer protein 2 protein protein ibvb ibvb IBV MLVTPLLLVTLLCALCSAVLYDSSSYVYYYQSAFRPPSGWHLQGGAYAVVNISSEFNNAGSSSGCTVGIIHGGRVVNASSIAMTAPSSGMAWSSSQFCTAHCNFSDTTVFVTHCYKHGGCPLTGMLQQNLIRVSAMKNGQLFYNLTVSVAKYPTFRSFQCVNNLTSVYLNGDLVYTSNETIDVTSAGVYFKAGGPITYKVMREVKALAYFVNGTAQDVILCDGSPRGLLACQYNTGNFSDGFYPFTNSSLVKQKFIVYRENSVNTTCTLHNFIFHNETGANPNPSGVQNIQTYQTKTAQSGYYNFNFSFLSSFVYKESNFMYGSYHPSCKFRLETINNGLWFNSLSVSIAYGPLQGGCKQSVFKGRATCCYAYSYGGPSLCKGVYSGELDHNFECGLLVYVTKSGGSRIQTATEPPVITQNNYNNITLNTCVDYNIYGRTGQGFITNVTDSAVSYNYLADAGLAILDTSGSIDIFVVQGEYGLNYYKVNPCEDVNQQFVVSGGKLVGILTSRNETGSQLLENQFYIKITNGTRRFRRSITENVANCPYVSYGKFCIKPDGSIATIVPKQLEQFVAPLFNVTENVLIPNSFNLTVTDEYIQTRMDKVQINCLQYVCGSSLDCRKLFQQYGPVCDNILSVVNSVGQKEDMELLNFYSSTKPAGFNTPVLSNVSTGEFNISLLLTNPSSRRKRSLIEDLLFTSVESVGLPTNDAYKNCTAGPLGFFKDLACAREYNGLLVLPPIITAEMQALYTSSLVASMAFGGITAAGAIPFATQLQARINHLGITQSLLLKNQEKIAASFNKAIGHMQEGFRSTSLALQQIQDVVSKQSAILTETMASLNKNFGAISSVIQEIYQQFDAIQANAQVDRLITGRLSSLSVLASAKQAEYIRVSQQRELATQKINECVKSQSIRYSFCGNGRHVLTIPQNAPNGIVFIHFSYTPDSFVNVTAIVGFCVKPANASQYAIVPANGRGIFIQVNGSYYITARDMYMPRAITAGDVVTLTSCQANYVSVNKTVITTFVDNDDFDFNDELSKWWNDTKHELPDFDKFNYTVPILDIDSEIDRIQGVIQGLNDSLIDLEKLSILKTYIKWPWYVWLAIAFATIIFILILGWVFFMTGCCGCCCGCFGIMPLMSKCGKKSSYYTTFDNDVVTEQYRPKKSV 0BAAD58113C8EBD5 rna_ibv Infectious bronchitis virus complete genome rna ribonucleic acid RNA rna ibvb ibvb IBV ACTTAAGATAGATATTAATATATATCTATTGCACTAGCCTTGCGCTAGATTTCCAACTTAACAAAACGGACTTAAATACCTACAGCTGGTCCCCATAGGTGTTCCATTGCAGTGCACTTTAGTGCCCTGGATGGCACCTGGCCACCTGTCAGGTTTTTGTTGCTAAAATATCATTGTTGCTGGTATCACTGCTTGTTTTGCCGTGTCTCACTTTATACATCCGTTGCTTGGGCTACCTAGTATCCAGCGTCCTACGGGCGCCGTGGTCGGTTCGAGTGCGAAGGACCTCTGGTTCATCTAGCGGTAGGCGGGTGTGTGGAAGTAGCGCTTCAGACGTATTGGTTCTGTTGCGTGAAACGCGGGGTCACCTCCCCCCACATACCTCTAAGGGCTTTTGAGCCTAGCGTTGGGCTACGTTCTCGCACAAGGTCGGCTATACGACGTTTGTAGGGGGTAGTGCCAAACAACCCCTGAGGTGACAGGTTCTGGTGGTGTTTAGTGAGCAGACATACAATAGACAGTGACAACATGGCTTCAAGCCTAAAACAGGGAGTATCTCCCAAACCAAGGGATGTCATTCTTGTTTCCAAAGACATTCCCGAACAACTCTGTGACGCTTTATTTTTCTACACGTCACATAACCCTAAGGATTACGCTGATGCTTTTGCATTTAGGCAAAAGTTTGACCGTAATCTGCAGACTGGGAAGCAGTTCAAATTTGAAACTGTTTGTGGTCTCTTCCTATTGAAGGGAGTTGACAAAATAACACCTGGCGTCCCAGCAAAAGTTTTAAAAGCCACTTCTAAGTTGGCAGATTTAGAAGACATCTTTGGTGTCTCTCCTTTTGCACGGAAGTACCGTGAATTGTTGAAAACAGCATGCCAGTGGTCTCTTACTGTAGAAACACTGGATGCTCGTGCACAAACGCTTGACGAAATTTTTGACTCTACTGAAATACTTTGGCTTCAGGTGGCTGCAAAAATTCAAGTTTCAGCTATGGCAATGCGCAGGCTTGTTGGAGAAGTAACTGCAAAAGTCATGGAAGCTCTTGGCTCAAATTTGAGTGTTCTCTTTCAAATTGTTAAACAACAAATAGCCAGAATCTTTCAAAAGGCACTGGCTATTTTTGAAAATGTGAGTGAATTACCACAGCGTATTGCAGCACTTAAGATGGCCTTTGCCAAGTGTGCCAAGTCAATTACTGTTGTGGTTGTGGAAAGAACTCTAGTTGTTAGAGAGTTCGCAGGAACTTGTCTTGCAAGCATCAATGGTGCTGTTGCAAAATTCTTTGAAGAACTTCCAAATGGCTTCATGGGTTCTAAAATCTTCACAACATTGGCCTTCTTTAAAGAAGCAGCTGTGAAAATTGTGGAAAATATACCAAATGCACCAAGAGGTACTAGAGGTTTTGAAGTCGTTGGTAACGCCAAGGGAACGCAAGTTGTTGTGCGTGGCATGCGAAATGATTTAACTCTGCTCGACCAAAAAGCTGACATTTCTGTTGAGAAAGAAGGTTGGTCTGCAATTCTTGAAGGACATCTGTGTTATGTCTTTAAGAGTGGTGATCGTTTTTATGCGGCACCTCTTTCTGGGAATTTTGCATTGCATGATGTGCATTGTTGTGAGCGTGTTGTCTGTCTGTCTGATGGTGTAACACCAGAGATAAATGATGGACTCATTCTAGCAGCAATCTATTCATCTTTTAGTGTCTCAGAACTCGTGGCAGCACTTAAAAAGGGTGAACCATTCAAGTTCTTGGGTCATAAATTTGTGTATGCGAAGGATGCAGCAGTCTCTTTCACTCTTGCAAAAGCAGCCACTATTGCAGATGTACTGAAGCTGTTTCAATCAGCTCGTGTGCAAACGGAAGATGTGTGGTCTGCATTTACTGAAAAGTCTTTTAATTTCTGGAAACTCGCATATGGAAAAGTGCGTAATCTTGAAGAAGTTGTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTATCATTCTAGCAGCAGTGCTTGGCGAAGGCATTTGGCATCTTGTTTCACAGGTCATCTATAAAGTAGGTGGTCTTTTTACTAGAGTCGTTGACTTTTGTGAAAAACACTGGAAGGGTTTCTGTGCACAACTTAAAAAGGCTAAGCTCGTTGTCACAGAAACTCTTTGTGTTCTTAAGGGAGTGGCACAGCATTGTTTTCAACTATTGCTGGATGCAATACATTCTTTGTATATGAGTTTTAAGAAGTGTGCACTTGGTAGAATTCATGGAGACTTACTCTTCTGGAAAGGGGGTGTACACAAAATTGTTCAAGATGGCGATGAAGTTTGGTTTGACGCCATTGATAGTATTGATGTTGAAGATCTGGGTGTTGTCCAAGAAAAACCCATAGATTTTGAGGTTTGTGAAGACGTAACACTTCCAGAAAATCAACCTGGTCATATGGTTCAAATCGAGGATGACGGAAAGAACTATATGTTCTTCCGCTTCAAAAGGGATGAGAACATCTACTATACACCAATGTCTCAACTTGGTGTAATTAATGTAGTTTGCAAAGCAGGCGGTAAAACCGTTACCTTTGGAGACACCATTGTGAAAGAAATACCGCCACCTGATGTTGTGCCTATTAAGGTTAGCATAGAGTGTTGTGGTGAACCATGGAATACAATCTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAAACTGACCTCACAGTAGAACAATTGCTCTCTGTGATCTATGAGAAAATGTGCGACGACCTCAAATTGTTTCCAGAGGCACCAGAGCCACCGCCATTTGAGAATGTCGCACTTGTTGATAAAAACGGTAAAGACTTGGATTGCATAAAATCATGCCATCTTATCTACCGTGATTATGAGAGTGATGATGACATCGAGGAAGAAGATGCTGAGGAGTGTGATACTGATTTAGAATGTGAAGAAGAGGATGAGGATACTAAAGTGTTGGCTCTTATACAAGACCCTGCAAGTAATAAATACCCTCTTCCTCTTGATGATGATTATAGCGTCTTTAATGGATGTATTGTACATAAGGACGCTCTTGACGTCGTAAATCTACCATCTGGTGAAGAAACCTTTGTTGTCAACAACTGCTTTGAGGGAGCTGTAAAACCACTGCCTCAGAAAGTTGTTGATGTTCTAGGTGACTGGGGTGAGGCTGTTGATGCGCAAGAGCAAATTGCACAAACTACTTCAGAGGAAACCCCTATCAGTAGTTTGGAGGCAACTATTGAGCAAGTTGTTGTTGAGGAACAGAAAATAATTTCTGTTGTTGAAGAAGAACAGCAGGTGGCGGTCTACACACCTGCAGACCTACAAGTTGGTGAAGAAACACCAGATGAGTTTATTCTTATTGCTGATGTTTCCACAGAAGAAATTGTGCCTCATGAAGAAAAGGAGTCACAGATTGAACAGGAGCCTATTCAAGTTGTTAAATCACAACGTGAAAAGAAGGCTAAAAAGTTCAAGGTTAAATCTACTACATGTGAGAAACCCAAATTTTTGGAGTACACAACATGTGTGGGTGACCTAACGGTAGTGATTGCCAAAGCATTGGATGAGTTTAAAGAGTTCTGCATTGTAAATGCTGCTAATGAGCATATGTCTCACGGTGGCGGCGTTGCTAAGGCAATTGCGGACTTTTGTGGACCTGATTTTGTGGAGTATTGTGAGGACTATGTTAAGAAACATGGGCCTCAACAAAGACTTGTCACACCTTCATTTGTCAAAGGCATTCAATGTGTGAACAATGTTGTAGGACCTCGCCATGGAGACAGTAACTTGCATGATAAGCTTGTTGCTGCTTACAAGAATGTTCTTGTAGATGGTGTTGTCAATTATGTTGTGCCAGTCCTCTCATCAGGAATTTTTGGTGTTGATTTTAAGATGTCTATAGACGCTATGCGCAAGGCTTTTGAAGGTTGCGACATACGCGTTCTTCTTTTCTCCTTGTCTCAAGAACACATCGATTATTTCGATGTTACTTGTAAACAGAAGACAATTTATCTTACAGAGGACGGTGTTAAATACCGCTCTGCTACTGTGAAACCAGGTGACTCTTTGAGTCAATTTGGACCGGTTTTTGCTAGAAACAAGACAGTCTTTACAGCAGACGATGTTGAGGATAAAGAAATTCTCTTCATTCCTACTACTGACAAGACTGTCCTTGAATATTATGGTTTGGATGCGCAAAAGTATGTAATATACTTGCAAACTCTTGCACAGAAGTGGAATGTCCAATATAGGGACAATTTTGTTATACTTGAGTGGCGTGATGGAAATTGCTGGATTAATGCAGCAGTAGTGCTCCTTCAAGCTGCTAAGATTAGGTTTAAAGGTTTTCTTGCAGAAGCATGGGCACAACTTTTGGGTGGAGACCCAACTGATTTTGTGGCCTGGTGCTATGCAAGTTGCAATGCTAATGTTGGTGAGTTTTCAGATGCTAATTGGCTTCTTGCTAATTTGGCAGAATACTTTGATGCTGATTACACGAATGCATTCCTTAAGAGGCGTGTGTCATGTAACTGTGGGGTTAAGAATTGTGAAGTTAGAGGCCTTGAAGCTTGTATTCAACCAGTAAAGGCACCCAATCTTCTTCATTTTAAGACTCAGTACACAAATTGTACAGTGTGTGATGCAAATATTGTGGATGAGGTGGTAGAAGCCTCACTACCATATCTGTTGCTCCTTGCTACTGATGGTCCTACTACAGTGGATTGTGATGAAAATGCTGTAGGGAATGTTGTTTTCATTGGCTCTACTAATAGTGGCCATTGTTACACGCAAGCCATTGGTAAGGCTTTTGATAATCTTGCTAAGGATAGAAAATTTTCAAAGAATTCGCCATACATTACAGCAATGTATACGCGCTTCTCTCTTAAGAGTGAAAGCTCTCTGTCTGTTGTTAAACAGAGTAAGAGTAAAACTAAAGTAGTAAAAGAAGATGTTGCCAACCTTGCTACTAGTTCTAAAGCCAGTTTTGATGATCTTACTGACTTTGAACATTGGTATGATAGTAACATCTATGAAAGTCTTAAAGTTCAGGAAATACCTGTGAATTTGGATGAGTATGTGTCATTTACAACGAAAGAAGATACTAAGTTGCCACTGACACTTAAAGTTAGAGGTATCAAATCAGTTGTTGACTTTATTTCAAGAGACGGTTTCTCTTATAAGTTAACACCTGACATTGAAGAAAATTCAAAAGCGCCAGTCTACTACCCAGTCTTAGACTCTATTAGTCTTAAGGCAATATGGGTAGACGGCAGTGCTAATTTTGTTGTTGGTCATCCAAACTACTATAGTAAGTCTCTTCGCATTCCTACTTTTTGGGAAAATGCAGAGAGCTTTGTTAAGATAGGTGACAAAGTTGATGGTGTAACTATGGGCCTTTGGCGTGCAGAACATCTTAACAAACCTAATCTTGAAAGAATTTTCAACATTGCTAAGAAAGCTATTGTTGGATCCAGTGTTGTTACTACACAATGTAGTAAATTAATTAGTAAAGCAGCTACATTCATTGCTGATAAAGTAGGTGGGGGTGTAGTTCGTAATATTACAGATAGAATTAAGGGTCTTTGTGGATTTACACGTGGGCATTTTGAAAGAAAATTGTCTCCACAATTCATAAAAACACTTATATTCTTCTTCTTTTACTTTGTAAAGGCTAGTGCTAAGAGTGTTGCCACTAGTTATAAGCGTGTGTTATGTAAGGTGGTTTTTACCACGCTATTTATATTATGGTTTATGTACACAAGTAAACCAGTAACTTTTACTGGAACACGTGTGCTAGACTTCTTATTTGAGGGTTCTTTATGTGGTCCCTATAATGACTATGGTAAAGACTCATTTGACGTACTACGCTATTGTGGAGATGATTTTACTTGTCGTGTATGTTTACATGATAAAGATTCACTTCATTTGTATAAGCATGCTTATAGCGTAGAACAGGTTTATAAATATGCAGCTTCTGGCATTAGTTTTAATTGGAATTGGCTTTATTTGGTCTTTCTAATATTATTTGTTAAACCAGTAGCAGGTTTCGTTATTATTTGCTATTGTGTTAAGTACTTGGTATTGAGTTCAACTGTGTTGCAAACTGGTGTAGGTTTTATGGACTGGTTTATTCAAACAGTTTTTACTCACTTTAATTTTATGGGTGCAGGTTTCTATTTCTGGCTCTTCTATAAATTGTACATACAGGTTCATCATATACTGTATTGTAAGGATATAACATGTGAAGTGTGTAAGAGAGTTGCACGCAGTAATAGGCATGAGGTTAGTGTTGTTGTTGGTGGACGCAAGCAAATTGTGCACGTGTACACTAACTCTGGTTACAACTTTTGTAAGAGACATAATTGGTATTGTAGGAATTGTGATGTATATGGTCACCAAAACACATTTATGTCTCCTGAAGTTGCTGGCGAGCTTTCTGAAAAGCTTAAACGCCATGTTAAACCTACAGCACATGCTTACCACGTTGTGGATGAGGCTTGCGTAGTTGATGATTTTGTTAACTTAAAATACAAAGCTGCAACTCCTGGTAAGGATGGTGCACCTCCTGCAGTTAAATGTTTCAGTGTTACAGATTTCTTGAAGAAAGCTGTTTTTCTTAAGGATGCGCTGAAATGTGAACAAATATCTAATGATGGTTTTATAGTGTGTAATACGCAGAGTGCGCATGCTTTAGAGGAAGCAAAGAATGCAGCCATCTATTATGCGCAATACCTGTGTAAACCTATACTTATACTCGACCAGGCACTCTACCAGAATTTAATAGTGGAACCTGTATCGAAGAGCGTTGTCAACAAAGTGTGTGACATTTTGTCTAGGATAATTTCTGTAGATACTGCATCTTTGGATTATAAAGCAGGTACAATTCGTGATGCCTTGCTGTCTGTTACTAAAGATGAAGAAGCTGTAGATATGGCTATCTTCTGTCATAATCATGAAGTTGAATATACAGGTGATGGTTTTACTAATGTTATACCGTCATATGGTATAGACACTGATAAATTAACACCTCGTGATAGAGGGTTTTTGATAAATGCAGATGCTTCTGTTGCTAACTTAAGAGTTAAAAATGCTCCGCCGGTAGTATGGAAGTTCTCTGATCTTATTAAGTTGTCTGACAGTTGTCTTAAATATTTAATCTCAGCAACTGTCAAGTCAGGGTCTCGTTTCTTTATAACAAGATCTGGTGCTAAACAAATTTTTTCTTGTAGTACTCAGAAATTGTTGGTAGAGAAAAAGGCTGGTGGTGTCGTTAGTGGTACCTTTAATTGGTTTAAGAGTTGTTGTAAATGGCTCTTGATCTTCTATGTGCTTTTTACATTGTGTTGTTTGGGTTGTTATCATATGGAGACGAATAAAAGTTTTGTTCATCCTATGTATGATGTTAACTCTACAATGCATGTTGAAGGCTTTAAGGTTATAGATAAAGGTGTTATTAGAGACATTGTACCAGAGGATGCTTGTTTCTCTAATAAGTTTGCTAACTTTGATGCATTTTGGGGTAAACCATATGTGAATAGTAGAGACTGTCCAATTGTTACAGCAGTCATAGATGGCGCTGGAACAATAGTAGCTGGTGTTCCTGGTTTTGTAGACTGGGTTCTTGATGGTGTTATGTTTGTACACATGACACAAACAGAAAGAAAACCCTGGTACATTCCCATGTGGTTTAACAGAGAAATTGTTGGTTACACTCAGGATTCAATTATTACTGAAGGTAGTTTTTATACATCTATAGCTTTGTTTTCAGCTAGGTGTTTATATTTAACAGCCAGCAATACACCACAATTGTATTGTTTTAATGGTCATAATGATGCTCCTGGAGCCTTACCATTTAGCAGTATCACTTCACACAGGGTCTACTTCCAACCAAATGGTGTTAGGCTTATAATTCCTCAACAAATAATGCACACACCCTACGTAGTAAAGTTTTTATCAGACAGCTATTGTAGAGGTAGTGTATGTGAGTATACTAAACCGGGTTATTGTGTTTCACTAAATTCCCAATGGGTTTTATTTAATGACGAATACACAAGTAAACCAGGAGTATTCTGTGGTTCTACTGTTAGAGAACTTATGTTTAATATGGTTAGTACATTTTTTACTGGTGTCAACCCTAATATTTATATGCAGCTGGCGACTATGTTTTTAATACTAGTTGTTGTTGTGTTAATTTTTGCAATGGTTATAAAGTTTCAATGTGTTTTTAAAGCTTATGCAACCATTGTGTTTATAATAATGCTAGTTTGGGTTGTTAATGCATTTATTTTGTGTGTACATAGTTATAATAGTGTTGTGGCTGTTATACTACTAGTAATCTATTGTTATGCATCATTGGTTACAAGTCGTAATACTGCTATAATAATGCATTGTTGGCTTGTGTTTACCTTTGGTTTAATTGTACCCATATGGTTGGCGTGTTGCTACCTGGCATTTGTTTTATATATGTACACACCATTGTTTTTCTGGTGTTACGGTACTACTAAAAATACTCGTAAGTTGTATGATGGCAACGAGTTTGTTGGTAATTATGACCTTGCTGCGAAGAGCACTTTTGTTATTCGTGGTACTGAATTTGTTAAGCTTACGAATGAGATAGGTGATAAATTTGAATCCTATCTTTCTGCGTATGCTAGACTTAAATATTATTCAGGCACTGGCAGTGAGCAAGATTACTTGCAAGCCTGTCGTGCATGGTTAGCTTATGCTTTGGACCAATATAGAAATAGTGGTGTGGAAATTGTGTATACTCCACCACGTTACTCTATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAACTAGTTTTTCCTAGTAGTGCTGTTGAAAAGTGCATTGTTAGTGTCTCTTATAGAGGTAATAATCTTAATGGACTATGGCTAGGTGATACTATCTACTGTCCGCGACATGTTCTAGGCAAGTTTTCAGGTGATCAATGGAGTGATGTACTTAATCTTGCTAATAATCATGAGTTTGAGGTTGCAACTCAAAATGGTGTTACTTTGAATGTTGTTAGTAGGCGGTTGAGAGGCGCAGTTTTAATTTTACAAACTGCTGTCGCCAATGCTGACACTCCTAAGTATAAGTTTGTTAAAGCTAATTGTGGTGATAGTTTCACTATAGCTTGTTCTTATGGTGGTACAGTTGTGGGACTCTACCCTGTTACTATGCGTTCTAATGGTACTATTAGAGCTTCTTTCCTTGCAGGAGCTTGTGGCTCAGTTGGTTTTAATATAGAGAAGGGTGTAGTTAATTTCTTTTATATGCACCATCTTGAGTTACCTAATGCATTACACACTGGAACTGACCTAATGGGTGATTTCTATGGTGGTTATGTGGACGAAGAGGTTGCACAAAGGGTGCCACCAGATAATTTAGTTACTAATAATATTGTAGCATGGCTTTATGCCGCAATTATTAGTGTTAAGGAGAGTAGTTTCTCACTGCCTAAATGGTTGGAGAGTACTACTGTCAGTGTTGAAGACTATAATAAGTGGGCTGGTGATAATGGTTTTACACCATTTTCTACTAGTACTGCTATTACTAAATTAAGTGCTATAACGGGAGTAGATGTTTGTAAACTCCTTCGCACTATTATGGTAAAAAGTAGTCAATGGGGTAGTGATCCCATTTTAGGACAATATAATTTTGAAGATGAATTGACACCAGAGTCTGTTTTCAACCAGATAGGTGGTGTTAGGTTACAGTCATCTATTGTAAGAAGAGTCACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGCTTCTTATTTGTGTTGTGTGCTATTGTCTTGTTTACGGCAGTACCACTTAAATACTATGTACATGCAGCTGTTATTTTGTTAACAGCTGTACTTTTTATTTCTTTTACTGTTAAACATGTTATGGCATATATGGATACTTTTCTGTTGCCTACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTCCCTTTCATATACAATACTCTAATTAGTCAAGTTGTTATTTTCTTAAGCCAATGGTATGATCCTGTAGTCTTTGATACTATGGTACCATGGATGTTATTGCCATTAGTGTTGTACACTGCTTTTAAGTGTGTACAAGGTTGCTATATGAATTCTTTCAATACTTCTTTGTTAATGCTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTATACCTCTTCTAACACTCTTACTGCATATACAGAAGGTAATTGGGAGTTATTTTTTGAGTTAGTTCACACTACTGTGTTGGCTAATGTTAGTAGCAATTCTTTAATTGGTCTACTTGTGTTTAAGTGTGCTAAGTGGATGTTGTATTATTGCAATGCAACATACTTTAATAATTATGTGTTAATGGCAGTCATGGTTAATGGCATAGGCTGGCTTTGTACTTGTTACTTTGGATTGTATTGGTGGGTTAATAAGGTTTTTGGTTTAACTTTAGGTAAATACAATTTTAAAGTCTCAGTAGATCAATATAGGTATATGTGTTTGCATAAGATAAATCCACCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGAATTGGTGGTGATCGTGTGTTGCCTATTGCTACAGTTCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATCCTTGCATCTGATGATGTTGGAGAGTGCATGGATAATTTGTTGGGTATGCTTATTACACTGTTTTGTATAGATTCTACTATTGATTTGAGTGAGTATTGTGATGATATACTTAAGAGGTCAACTGTCTTACAGTCAGTTACTCAAGAGTTCTCACACATACCCTCTTATGCTGAATATGAAAGAGCTAAGAATCTTTATGAAAAGGTTTTAACTGATTCTAAAAATGGTGGTGTAACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCAGTTTTTGATAGAGACTTGGCTGTTCAAAAGAAGTTAGACAGCATGGCAGAACGTGCTATGACAACAATGTATAAAGAGGCGCGTGTAACTGATAGACGAGCAAAATTAGTTTCATCACTACATGCGTTACTCTTTTCAATGCTTAAGAAAATAGATTCTGAAAAGCTTAATGTCTTATTTGATCAGGCTAGTAGCGGTGTTGTACCTCTAGCTACTGTTCCAATTGTTTGTAGTAATAAGCTTACCCTTGTAATACCAGATCCAGAAACTTGGGTCAAGTGTGTGGAAGGTATGCATGTTACATATTCAACAGTTGTTTGGAATATAGACACTGTTATTGATGCTGATGGTACAGAGTTACATCCAACTTCTATAGGTAGTGGATTGACATACTGTATAAGTGGTGACAATATAGCATGGCCTTTAAAGGTCAACTTGACTAGGAATGGGCATAACAAGGTTGATGCTGCTTTGCAGAATAATGAGCTTATGCCTCATGGTGTAAAAACAAAGGCTTGCGTAGCAGGTGTAGATCAAGCACATTGTAGCGTAGAGTCTAAATGTTATTATACAAATATTAGTGGCAATTCAGTTGTAGCTGCTATTACTTCTTCAAATCCAAATCTGAAAGTAGCTTCGTTTTTGAACGAGGCAGGCAATCAGATTTATGTAGACTTAGACCCACCATGTAAATTTGGCATGAAGGTGGGTGACAAGGTTGAGGTTGTTTACTTGTATTTTATAAAGAATACAAGGTCGATTGTTAGGGGTATGGTACTTGGTGCTATATCTAATGTTGTTGTCTTACAGTCTAAAGGGCATGAAACAGAGGAAGTGGATGCTGTTGGCATTCTTTCACTTTGCTCATTTGCAGTAGATCCCGCTGATACATATTGTAAATATGTGGCGGCAGGTAATCAACCTTTAGGTAACTGTGTTAAAATGTTGACAGTACATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGTCCAACTCCTGATCAGGATTCTTATGGAGGAGCTTCTGTGTGTCTCTATTGTAGAGCACACATAGCACACCCAGGAGGTGCAGGAAATTTAGATGGACGTTGTCTATTTAAAGGTTCTTTTGTGCAAATACCTACTACTGAGAAAGACCCCGTCGGATTCTGTCTACGTAATAAGGTTTGTACTGTTTGTCAGTGTTGGATTGGTTATGGCTGTCAGTGCGATGCACTTAGACAACCTAAACCTTTTGTTCAGTCAGTTGCTGGTGCATCTGATTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGATACCCCTTGCTAGTGGATGTGATCCTGATGTTGTAAAGCGAGCCTTTGATGTTTGTAATAAGGAATCATCTGGTATGTTTCGAAACTTTAAGCGTAACTGTGCGAGATTCCAAGAAGTACGTGATACTGAAGATGGAAATCTTGAGTATTGTGATTCGTACTTTGTGGTTAAACAAACCACTCCTAGTAATTATGAACATGAGCGGTCTTGTCATGAAGACTTAAAGTCAGACGTAATAGCCGATCATGATTTCTTTGTGTTCAATAAGAACATTTATAATATTAGTAGGCAGAGGCTTACTAAATATACTATGATGGACTTTTGCTACGCTTTGAGGCATTTTGACCCAAAGGACTGCGAAGTTCTTAAAGAAATACTTGTCACTTATGGTTGTATAGAAGATTATCACCCTAAGTGGTTTGAAGAGAATAAGGATTGGTACGACCCAATAGAAAACCCAAAATATTATGCCATGTTGGCTAAAATGGGGCCTATTGTACGACGTGCTCTATTGAATGCTATTGAGTTCGGAAACCTTATGGTTGAAAAAGGTTATGTTGGTGTTGTTACACTTGATAACCAAGATCTTAACGGTAAATTTTATGATTTTGGTGATTTTCAAAAAACAGCACCTGGTGCTGGTGTTCCTGTTTTTGATACATATTATTCTTACATGATGCCCATCATAGCCATGACGGATGCTTTGGCACCTGAAAGGTATTTTGAATATGATGTGCATAAGGGTTATAAGTCTTATGATCTCCTCAAGTATGATTATACTGAGGAGAAACAAGAGTTGTTTCAGAAATACTTTAAGTATTGGGACCAGGAGTACCATCCTAACTGCCGTGACTGTATTGATGACAGGTGTTTGATACATTGTGCAAACTTCAACATCTTGTTTTCTACACTGATACCGCAGATTTCTTTTGGTAATTTGTGTAGAAAGGTGTTTGTTGATGGTGTACCTTTTATAGCTACTTGTGGCTATCATTCCAAAGAACTTGGTGTTATTATGAATCAAGATAACACTATGTCGTTCTCAAAAATGGGTTTAAGTCAACTCATGCAGTTTGTTGGAGACCCTGCCTTGTTAGTGGGAACATCCAATAATTTAATCGATCTTAGAACGTCTTGTTTTAGTGTTTGTGCATTGGCGTCTGGTATTACTCATCAAACGGTAAAACCAGGTCACTTTAACAAGGATTTCTATGATTTTGCAGAGAAGGCTGGTATGTTTAAGGAAGGTTCTTCTATACCACTTAAACATTTCTTCTACCCTCAGACTGGTAATGCTGCTATAAACGATTATGATTATTATCGTTATAACAGGCCTACCATGTTCGATATACGTCAACTTCTATTTTGTTTAGAAGTGACTTCTAAATACTTTGAATGCTATGAAGGCGGCTGTATACCAGCAAGCCAAGTTGTAGTTAATAATCTAGATAAGAGCGCAGGCTACCCATTTAATAAGTTTGGAAAAGCCCGTCTCTATTATGAAATGAGTCTAGAGGAACAGGACCAACTCTTTGAGAGTACAAAGAAGAATGTCCTGCCCACTATAACTCAAATGAATTTAAAATATGCCATATCCGCGAAAAATAGAGCGCGTACAGTGGCAGGTGTGTCTATCCTTTCTACTATGACTAATAGGCAGTTTCATCAGAAGATTCTTAAGTCTATAGTCAACACTAGAAACGCTCCTGTAGTTATTGGAACAACCAAGTTTTATGGCGGTTGGGACAATATGTTGAGAAACCTTATTCAGGGTGTTGAAGATCCGATTCTTATGGGTTGGGACTATCCAAAGTGTGATAGAGCAATGCCAAATTTGCTACGTATAGCAGCATCTTTGGTACTTGCTCGGAAACACACTAACTGTTGTACTTGGTCTGAGCGCATTTATAGGTTGTATAATGAATGCGCTCAGGTTTTATCAGAAACTGTCCTAGCTACAGGTGGTATTTATGTAAAACCTGGTGGTACTAGCAGTGGTGATGCTACTACTGCTTATGCAAACAGTGTTTTTAATATAATACAAGCTACATCTGCTAATGTTGCGCGTCTTTTGAGTGTTATAACGCGTGATATTGTTTATGATGACATTAAGAGCCTGCAGTATGAGTTGTACCAGCAGGTTTATAGGCGAGTTAATTTTGACCCAGCCTTTGTAGAAAAGTTTTATTCTTACTTATGTAAGAATTTCTCTTTGATGATCTTGTCCGACGACGGTGTTGTTTGTTATAACAATACACTAGCCAAACAAGGTCTTGTAGCAGATATTTCTGGTTTTAGAGAAGTTCTCTACTACCAAAATAATGTCTTTATGTCTGACGCTAAATGTTGGGTGGAACCAGATTTAGAAAAAGGCCCTCATGAATTTTGTTCACAGCATACAATGCTAGTGGAAGTGGATGGTGAGCCTAAATACTTGCCATATCCAGACCCTTCACGCATTTTAGGTGCATGTGTTTTTGTAGATGATGTGGATAAGACGGAACCTGTGGCTGTTATGGAGCGTTATATAGCTCTAGCCATAGACGCTTACCCGCTAGTACATCATGAAAATGAGGAGTACAAGAAGGTGTTCTTTGTGCTTCTTTCATACATCAGAAAACTCTATCAAGAGCTTTCTCAGAATATGCTTATGGACTACTCTTTTGTAATGGATATAGACAAGGGTAGTAAATTTTGGGAACAGGAGTTCTATGAGAATATGTATAGAGCTCCTACGACTTTACAATCTTGTGGTGTCTGTGTAGTTTGTAATAGTCAAACTATACTGCGCTGTGGTAATTGTATTCGCAAACCATTTTTGTGTTGTAAATGTTGCTATGACCATGTCATGCATACAGACCACAAAAATGTTTTGTCTATAAATCCATACATTTGCTCACAGCCCGGTTGTGGCGAGGCAGATGTTACTAAATTGTACCTCGGAGGTATGTCATACTTCTGTGGTAATCATAAACCAAAATTGTCAATACCGTTGGTATCTAATGGTACTGTTTTTGGAATTTACAGGGCTAATTGTGCTGGTAGCGAAAGTGTTGATGATTTTAATCAACTAGCTACTACTAATTGGTCTACTGTGGAACCTTATATTTTGGCAAATCGCTGTAGTGACTCATTGAGACGCTTTGCTGCGGAAACAGTAAAAGCTACAGAGGAGTTGCATAAGCAGCAGTTTGCTAGTGCTGAAGTGCGAGAAGTTCTCTCAGATCGTGAGTTGATTCTATCATGGGAGCCAGGTAAAACTAGGCCTCCATTGAATAGGAATTATGTCTTTACAGGCTATCACTTTACAAGAACTAGTAAGGTGCAGCTTGGTGATTTTACATTTGAAAAAGGTGAAGGTAAAGATGTTGTCTATTATAGGGCAACGTCCACTGCTAAATTGTCTGTTGGAGACATTTTTGTTTTAACTTCACACAATGTTGTTTCTCTTGTAGCACCAACATTGTGTCCACAACAGACCTTTTCTAGGTTTGTAAACTTAAGACCTAATGTAATGGTACCAGAATGTTTTGTGAACAACATTCCACTCTACCATTTAGTAGGTAAGCAGAAGCGTACTACAGTACAAGGTCCCCCAGGCAGTGGTAAATCACATTTTGCTATAGGCCTTGCAGCATACTTTAGTAACGCTCGTGTTGTCTTTACTGCATGTTCTCATGCAGCTGTTGATGCTTTATGTGAAAAAGCTTTTAAGTTTTTAAAAGTTGATGATTGCACTAGGATAGTACCTCAAAGAACTACTATCGACTGCTTTTCAAAGTTTAAAGCTAATGACACAGGCAAAAAGTATATTTTTAGTACTATAAATGCCTTGCCAGAAGTTAGTTGTGACATTCTTTTGGTTGACGAGGTTAGTATGTTGACCAATTATGAATTGTCTTTTATTAATGGTAAGATAAACTACCAATATGTTGTGTATGTAGGTGATCCCGCTCAATTACCGGCACCTCGTACCTTACTTAATGGTTCACTTTCACCAAAGGATTATAATGTTGTAACAAACCTTATGGTTTGCGTTAAACCCGATATCTTCCTTGCGAAGTGTTACCGTTGTCCTAAGGAAATTGTAGACACTGTGTCTACTCTTGTTTATGATGGAAAGTTTATTGCAAATAACCCAGAATCACGTCAGTGTTTCAAGGTTATAGTTAATAATGGCAATTCTGATGTAGGACATGAAAGTGGTTCAGCCTACAACACAACTCAATTAGAATTTGTGAAAGATTTTGTTTGTCGCAATAAGGAGTGGCGGGAAGCAACATTCATTTCACCTTATAATGCTATGAACCAGAGAGCCTATCGTATGCTTGGACTTAATGTTCAGACAGTAGACTCGTCTCAAGGTTCAGAGTATGATTATGTTATATTCTGTGTTACAGCAGATTCGAATCATGCACTGAATATTAACAGATTCAATGTAGCGCTTACAAGAGCTAAGCGTGGTATACTAGTTGTCATGCGTCAGCGTGATGAATTGTATTCGGCTCTTAAGTTTACAGAGCTTGATAGTGAAACAAGTCTGCAAGGTACAGGTTTGTTTAAAATTTGCAACAAGGACTTTAGTGGTGTCCATCCTGCTTATGCAGTCACAACTAAGGCTCTTGCCGCAACTTATAAAGTTAATGATGAACTTGCTGCACTTGTTAATGTGGAAGCTGGTTCAGAAATAACATATAAACATCTTATTTCTCTTTTAGGATTTAAGATGAGTGTTAATGTTGAAGGCTGCCACAACATGTTTATAACACGTGAAGAGGCAATTCGTAATGTGAGAGGTTGGGTAGGTTTTGATGTAGAAGCTACACATGCTTGTGGTACTAACATCGGCACTAACTTGCCTTTTCAAGTAGGTTTCTCTACTGGTGCTGACTTTATAGTCACGCCTGAGGGAATTGTAGATACTTCAATAGGCAATAATTTTGAGCCTGTTAATTCTAAGGCACCTCCAGGTGAACAATTTAATCACTTAAGGGCTTTATTTAAAAGTGCTAAACCTTGGCATGTTATAAGACCAAGGATTGTACAAATGTTAGCAGACAACCTATGCAATGTTTCAGATTGCGTAGTTTTTGTAACTTGGTGTCATGGTCTAGAACTAACTACTTTGCGCTATTTTGTTAAAATAGGCAAAGAACAAGTATGTTCTTGTGGTTCTAGAGCTACAACATTTAATTCTCATACTCAAGCTTATGCTTGTTGGAAGCATTGTTTGGGTTTTGATTTTGTTTATAACCCACTTCTAGTGGATGTTCAACAGTGGGGTTACTCTGGTAACCTACAATTTAATCATGACTTGCACTGTAATGTGCATGGACACGCGCATGTTGCCTCTGCGGATGCTATTATGACGCGTTGTCTTGCAATTAACAATGCATTTTGTCAAGATGTCAACTGGGATTTGACATACCCTCATATTGCAAATGAGGATGAAGTCAATTCTAGTTGTAGATACTTACAACGCATGTATCTTAATGCATGTGTTGATGCTCTTAAAATTAACGTTGTCTATGATATAGGCAACCCTAAAGGTATAAAATGTGTTAGACGTGGAGACTTGAGTTTTAGATTCTATGATAAGAATCCAATAGTACCCAACGTCAAGCAGTTTGAGTATGACTATAATCAGCATAAAGATAAGTTTGCTGATGGTCTTTGTATGTTCTGGAATTGTAATGTGGATTGTTATCCTGATAATTCCTTGGTTTGCAGGTATGACACACGAAATTTGAGTGTGTTTAACTTACCAGGTTGTAATGGTGGTAGCCTGTATGTCAATAAACATGCATTCCACACACCTAAATTTGATCGCATTAGCTTTCGTAATTTGAAAGCTATGCCATTCTTTTTCTATGACTCATCTCCTTGCGAAACCATTCAAGTGGATGGAGTTGCACAGGATCTTGTGTCACTAGCTACTAAAGATTGTATCACAAAATGCAACATAGGCGGTGCTGTTTGTAAGAAACATGCGCAGATGTATGCAGAGTTTGTGACTTCTTATAATGCAGCGGTAACAGCTGGTTTTACTTTTTGGGTTACTAATAATTTTAACCCATATAATTTGTGGAAAAGTTTTTCAGCTCTCCAGTCTATCGATAACATTGCTTATAATATGTATAAGGGTGGTCATTACGACGCTATTGCAGGAGAAATACCCACCATCGTAACTGGAGATAAAGTTTTTGTTATTGATCAAGGTGTAGAAAAGGCAGTTTTTGTTAATCAAACAACACTGCCTACTTCTGTGGCGTTTGAACTGTATGCGAAGAGAAATATTCGCACACTGCCAAACAACCGTATTTTGAAGGGTCTTGGTGTAGATGTAACCAATGGTTTTGTAATTTGGGATTATGCGAACCAAACACCATTATATCGTAATACTGTTAAGGTATGTGCATACACAGACATTGAGCCAAATGGCCTAATAGTTCTGTATGATGATAGATATGGTGATTACCAATCTTTTCTTGCCGCTGATAATGCTGTTCTAGTTTCTACACAGTGTTATAAGCGATATTCATATGTAGAAATACCGTCAAACATGCTTGTTCAGAATGGTATGCCATTAAAAGACGGAGCGAATCTGTATGTCTATAAGCGCGTTAATGGAGCGTTTGTTACGCTACCTAACACACTAAACACACAAGGTCGCAGTTATGAAACTTTTGAACCTCGTAGCGACGTTGAGCGTGATTTTCTCGACATGTCGGAAGAGGATTTTGTAGAAAAGTATGGTAAAGACTTAGGTCTACAACACATACTGTATGGTGAAGTTGATAAACCACAATTGGGCGGTTTGCACACTGTTATAGGTATGTACAGACTTTTACGTGCGAATAAGTTGAATGCAAAGTCTGTTACTAATTCAGATTCTGATGTCATGCAAAATTATTTTGTGTTGGCAGATAATGGTTCTTACAAGCAAGTGTGCACTGTTGTGGATTTACTGCTTGATGATTTCTTAGAACTGCTTAGGAACATACTGAATGAGTATGGTACTAATAAGTCAAAAGTTGTAACAGTGTTAATTGATTACCATAGCATAAATTTTATGACTTGGTTTGAAGATGGCAGTATTAAAACATGTTATCCACAGCTTCAATCAGCATGGACGTGTGGTTATAATATGCCTGAACTCTATAAAGTCCAGAATTGTGTTATGGAACCTTGCAACATTCCTAATTATGGTGTTGGAATAACGTTGCCAAGTGGTATTATGATGAATGTGGCAAAGTACACACAACTTTGTCAATACCTTTCGAAAACAACAATGTGTGTGCCGCATAATATGCGCGTTATGCATTTTGGAGCTGGCAGTGATAAAGGAGTGGCTCCAGGTAGTACTGTTCTTAAACAGTGGCTTCCTGAAGGGACACTCCTTGTAGATAATGATATTGTAGATTATGTGTCTGATGCACATGTTTCTGTGCTTTCAGATTGCAATAAATATAAGACAGAGCACAAGTTTGATCTTGTGATATCTGATATGTATACAGACAATGATTCAAAAAGAAAGCATGAAGGCGTGATAGCCAACAATGGCAATGATGACGTTTTCATATATCTTTCAGACTTTCTTCGTAACAATTTGGCTCTTGGCGGCAGTTTTGCTGTAAAGGTGACAGAGACAAGTTGGCACGAGAATTTATATGACATTGCACAAGATTGTGCATGGTGGACAATGTTTTGTACTGCAGTGAATGCTTCTTCTTCAGAAGCATTTCTGGTTGGTGTTAATTATTTGGGTGCAAGTGAAAAGCTTAAAGTTAATGGAAAAACCCTGCACGCAAATTATATATTTTGGAGGAATTGTAATTATTTACAAACCTCAGCTTATAGTATATTTGACGTTGCTAAGTTTGATTTGAAATTAAAAGCAACGCCAGTTGTAAATTTGAAAACTGAACAAAAGACCGACTTAGTAGTTAATTTACTAAGGAACGGTAAATTGTTAGTTAGAGATGTTGGTGAAGTCACTGTTTCTAGTGACCATTTTGTTTGCACTATGTAGTGCTAATTTATATGACAACGAATCTTTTGTGTATTACTACCAGAGTGCTTTTAGGCCAGGACATGGTTGGCATTTACATGGAGGTGCTTATGCAGTAGTTAATGTGTCTAGTGAAAATAATAATGCAGGTACTGCCCCAAGTTGCACTGCTGGTGCTATTGGCTACAGTAAGAATCTCAGTGCGGCCTCAGTAGCCATGACTGCACCACTAAGTGGTATGTCATGGTCTGTCAACTCTTTTTGTACAGCCCACTGTAATTTTACTTCTTATATAGTGTTTGTTACACATTGTTATAAGAGCGGATCTAATAGTTGTCCTTTGACAGGTCTTATTCCAAGCGGTTATATTCGTATTGCTGCTATGAAACATGGAAGTGCTATGCCTGGTCACTTATTTTATAATTTAACAGTTTCTGTGACTAAATATCCTAAGTTTAGATCGCTACAATGTGTTAATAATTATACTTCTGTATATTTAAATGGTGACCTTGTTTTCACATCTAACTATACTGAAGATGTTGTAGCTGCAGGTGTCCATTTTAAAAGTGGTGGACCTATAACTTATAAAGTTATGAGAGAGGTTAAAGCCTTGGCTTATTTTGTCAATGGTACTGCACATGATGTCATTCTATGTGATGACACACCTAGAGGTTTGTTAGCATGCCAATATAATACTGGCAATTTTTCAGATGGCTTCTATCCTTTTACTAATACTAGTATTGTTAAGGATAAGTTTATTGTTTATCGTGAAAGTAGTGTCAATACTACTTTAACATTAACTAATTTCACGTTTAGTAATGAAAGTGGTGCCCCTCCTAATACAGGTGGTGTTGACAGTTTTATTTTATACCAGACACAAACAGCTCAGAGTGGTTATTATAATTTTAACTTTTCATTTCTGAGTAGTTTTGTTTATAGGGAAAGTAATTATATGTATGGATCTTACCATCCACGTTGTAGTTTTAGACCTGAAACTCTTAACGGTTTGTGGTTTAATTCCCTTTCTGTTTCATTAACATACGGTCCCATTCAAGGTGGTTGTAAGCAATCTGTATTTAATGGTAAAGCAACTTGTTGTTATGCTTATTCATACGGAGGACCTCGTGGTTGTAAAGGTGTCTATAGAGGTGAGCTAACACAGCATTTTGAATGTGGTTTGTTAGTTTATGTTACTAAGAGCGATGGCTCCCGTATACAAACTGCAACACAACCACCTGTATTAACCCAAAATTTTTATAATAACATCAATTTAGGTAAGTGTGTTGATTATAATATATATGGCAGAATTGGCCAAGGTCTTATTACTAATGTAACCGACTTAGCTGTTAGTTATAATTATTTATCAGACGCAGGTTTGGCTATTTTAGATACATCTGGTGCCATAGACATCTTCGTTGTACAAGGTGAATATGGTCCTAACTATTATAAGGTTAATCCATGTGAAGATGTCAACCAACAGTTTGTAGTTTCTGGTGGTAAATTAGTAGGTATTCTCACTTCACGTAATGAAACAGGTTCTCAGCTTCTTGAGAACCAGTTTTATATTAAAATCACTAATGGAACTCGTCGTTCTAGACGTTCTGTTACTGAAAATGTTACAAATTGCCCTTATGTTAGTTATGGCAAGTTTTGTATAAAACCTGATGGTTCAATTTCTGTAATAGTACCAAAAGAACTGGATCAGTTTGTGGCACCTTTACTTAATGTTACTGAATATGTGCTCATACCTAACAGTTTTAATTTAACTGTTACAGATGAGTACATACAAACGCGTATGGATAAGATCCAAATTAATTGCCTGCAGTATGTTTGTGGCAATTCTTTGGCCTGTAGAAAGCTGTTTCAACAATATGGGCCTGTTTGTGACAACGTATTGTCTGTAGTAAATAGTGTTGGTCAAAAAGAAGATATGGAACTTTTAAATTTCTATTCTTCTACTAAACCAGCTCGTTTTAATACACCAGTTTTTAGTAATCTTAGCACTGGTGAGTTTAATATTTCTCTTTTGTTAACATCCCCTAGTAGTCCTAGGAGGCGTTCTTTTATTGAAGATCTTTTATTTACAAGTGTTGAATCTTTAGGATTACCAACAGATGACGCATACAAAAAGTGCACTGCAGGACCTTTAGGCTTTTTTAAAGACCTTGCATGTGCTCGTGAATATAATGGTTTGCTTGTGTTGCCTCCTATTATAACAGCAGAAATGCAAACTTTGTATACTAGTTCTTTAGTAGCTTCTATGGCTTTTGGTGGTATTACTGCAGCTGGTGCCATACCTTTTGCCACACAACTGCAGGCTAGAATTAATCACTTGGGTATTACCCAGTCACTTTTGTTGAAGAATCAAGAAAAAATTGCTGCTTCCTTTAATAAGGCCATTGGTCATATGCAGGAAGGTTTTAGGAGTACATCTCTAGCATTACAACAAATTCAAGATGTTGTTAATAAGCAGAGTGCTATTCTTACTGAGACTATGGCAGCACTTAATAAAAATTTTGGTGCTATTTCTTCTGTGATTCAAGACATTTACCAGCAACTTGATTCCATACAAGCAGATGCTCAAGTGGATCGGCTCATAACTGGTAGATTGTCATCACTTTCTGTCTTAGCATCTGCTAAGCAGTCGGAGTACATTAGAGTGTCACAACAGCGTGAGTTAGCTACTCAGAAAATTAATGAGTGTGTTAAATCACAGTCTATTAGGTATTCCTTTTGTGGTAATGGACGACATGTTTTAACCATACCACAAAATGCCCCTAATGGTATAGTGTTTATACACTTTACTTATACACCAGAGAGCTTTATTAATGTTACTGCAATAGTGGGTTTTTGTGTAAGTCCTGCTAATGCTAGTCAGTATGCAATAGTGCCCGCTAATGGTAGGGGTATTTTTATACAAGTTAATGGTAGTTACTACATCACTGCACGAGATATGTATATGCCAAGAGATATTACTGCAGGAGATATAGTTACGCTTACTTCTTGTCAAGCAAATTATGTAAGTGTAAATAAGACCGTCATTACTACATTTGTAGACAATGATGATTTTGATTTTGATGATGAATTGTCAAAATGGTGGAATGATACTAAGCATGAGCTACCAGACTTTGACAAATTCAATTACACAGTACCTATACTTGACATTGATAGTGAAATTGATCGTATTCAAGGCGTTATACAGGGTCTTAACGACTCTCTAATAGACCTTGAAACACTATCAATACTCAAAACTTATATTAAGTGGCCTTGGTATGTGTGGTTAGCCATAGCTTTTGCCACTATTATCTTCATCTTAATACTAGGATGGTTGTTTTTCATGACTGGTTGTTGTGGTTGTTGTTGTGGATGCTTTGGCATTATTCCTTTAATGAGTAAGTGTGGTAAGAAATCTTCTTATTACACGACTTTTGATAATGATGTGGTAACTGAACAATACAGACCTAAAAAGTCTGTTTAATGATTCAAAGTCCCACATCTTTTCTAATAGTATTAATTTTTCTTTGGTGTAAACTTGCACTAAGTTGTTTTAAAGAGTGTGTTATAGCACTCCAGCAACTAATACAAGTTTTACTCCAAATTATTAATAGTAACTTACAGTCTAGACTTCTGCTTTGGCACAGTCTAGACTAATGTTAGATTTTGAAGCAATTATTGAAACTGGTCAGCAAATAATTCAACAAATCAGTTTCGATTTACAGCAAATTTCAAGTGTGCTAAGCACTGAATTATTTGACCCCTTTGAAGTCTGTGTTTACAGAGGAGGTAATTATTGGGAGTTAGAGTCAGCTGACGAGTTTTCAGGTGATGACGAATATATTGAGTAAATCGCTAGAGGAGAACGGAAGTTTCCTAACAGCAGTTTACATATTTGTTGGATTTTTAGTATTTTACCTATTAGGTAGAGCACTCCAAGCATTTGTACAAGCTGCTGATGCTTGTTGTTTATTTTGGTATACATGGGTAGTAGTTCCTGGAGCTAAGGGTACAGCCTTTGTGTATAATCATACATATGGTAAAAAACTTAACAAACCGGAGTTAGAAGCGGTTATTGTTAACGAGTTCCCCAAGAACGGTTGGAATAATAAAAGTCCAGCAAATTTCCAATATGATGGAAAATTGCACACTTAACTTAGAGCAGGCAACTCTGCTTTTTAAAGAATATAATTTATTTATAACCGCATTCCTATTGTTTCTTACTATACTACTTCAGTATGGGTACGCAACTAGGAGTCGGTTTATTTATATACTGAAAATGATAGTGTTATGGTGCTTTTGGCCCCTTAACATTGCAGTAGGTGTAATTTCATGTATATATCCACCAAATACAGGAGGTCTTGTCGCAGCGATAATACTTACTGTGTTTGCTTGTCTTTCTTTTGTAGGTTATTGGATTCAGAGTTGTAGACTCTTTAAAAGGTGTAGGTCTTGGTGGTCTTTTAACCCCGAGTCTAATGCCGTAGGTTCAATACTCCTCACAAATGGTCAACAATGTAATTTTGCTATAGAGAGTGTGCCTATGGTGCTTGCTCCAATTATAAAGAACGGTGTCCTTTATTGTGAGGGTCAGTGGCTTGCTAAATGTGAACCAGACCACTTGCCTAAAGACATATTTGTATGCACACCGGATAGACGTAATATCTATCGTATGGTGCAGAAATACACTGGTGACCAAAGCGGAAATAAGAAAAGGTTTGCTACATTTGTCTATGCAAAGCAGTCAGTAGACACTGGCGAGCTAGAAAGTGTAGCAACAGGAGGAAGTAGTCTTTACACATAAATGTGTGTGTGTAGAGAGTATTTAAGACTATTCTTTAATAGTGCCTCTATTTTAAGAGCGCATACGAGTATTTATTTTGAGGATATTAATATAAATCCTCTTTGTTTTATACTCTCTTTTCAAGAGCTATTATTTAAAAAACAGTTTTTCCACTCTTTTGTGCCAAAAACTATTGTTGTTAACGGTGTTACCTTTCAAGTGGATAATGGAAAAGTCTACTACGAAGGAACACCAGTTTTCCAAAAAGGTTGTTGTAGAATGTGGTCCAATTATAAGAAAGATTAGAATAATTAAGCCACCAACTACACTTATTTTTATAAGAGGCGTTTTATCTTACAAACGCTTAACAAATACGGACGATGAAATGGCTGACTAGTTTTGGAAGAGCAGTTATTTCATGTTATAAAGCCCTACTATTAACTCAATTAAGAGTGTTAGATAGGTTAATTTTAGGTCACGGACCAAAACGCGTTTTAACGTGTAGTAGGCGAGTGCTTTTGTTTCAGTTAGATTTAGTTTATAGGTTGGCGTTTACGCCCACCCAATCGCTGGTATGAATAATAGTAAAGATAATCCTTTTCGCGGAGCAATAGCAAGAAAAGCGCGAGTTTATCTGAGAGAAGGATTAGATTGTGTTTACTTTCTTAACAAAGCAGGACAAGCAGAGCCTTGTCCCGCGTGTACCTCTCTAGTATTCCAAGGGAAAACTTGTGAGGCACACATAAATAATAATAATCTTTTGTCATGGCAAGCGGTAAGGCAACTGGAAAGACAGACGCCCCAGCGCCAGTCATCAAACTAGGAGGACCAAAGCCACCTAAAGTTGGTTCTTCTGGAAATGCATCTTGGTTTCAAGCAATAAAAGCCAAGAAGCTAAATTCACCTCCACCTAAGTTTGAAGGTAGCGGTGTTCCTGATAATGAAAATCTTAAAATAAGCCAGCAACATGGATACTGGAGACGCCAAGCTAGGTTTAAGCCAGGTAAAGGCGGAAGAAAACCAGTCCCAGATGCTTGGTACTTCTATTATACTGGAACAGGACCAGCCGCTGACCTGAATTGGGGTGATAGCCAAGATGGTATAGTGTGGGTTGCTGCAAAGGGTGCTGATGTTAAATCTAGATCTAACCAGGGTACAAGGGACCCTGACAAGTTTGACCAATATCCACTACGATTCTCGGACGGAGGACCTGATGGTAATTTCCGTTGGGACTTCATTCCTCTGAATCGTGGTAGGAGTGGAAGATCAACAGCAGCTTCATCAGCAGCATCTAGTAGAGCACCGTCGCGTGACGGCTCGCGTGGTCGTAGAAGTGGTTCTGAAGATGATCTTATTGCTCGTGCAGCAAAGATAATCCAGGATCAGCAGAAGAAGGGTTCTCGCATTACTAAGGCTAAGGCTGATGAAATGGCTCATCGCCGGTATTGCAAGCGCATTATTCCACCTGGTTATAAGGTTGATCAAGTCTTTGGTCCCCGTACTAAAGGTAAGGAGGGAAATTTTGGTGATGACAAGATGAATGAGGAAGGTATTAAGGATGGGCGTGTTACGGCAATGCTCAACCTAGTCCCTAGCAGCCATGCTTGCCTTTTTGGAAGTAGGGTGACGCCCAAACTTCAACCAGATGGGCTTCACTTGAGATTTGAATTTACTACTGTGGTCCCGCGTGATGATCCGCAGTTTGATAATTATGTGAAAATTTGTGACCAGTGTGTTGATGGTGTAGGAACACGTCCAAAAGATGACGAACCGAGACCAAAGTCACGCTCAAGTTCAAGACCTGCTACAAGAACAAGTTCTCCGGCGCCAAGACAACAACGCCCAAAGAAGGAGAAAAAGTCAAAGAAGCAGGATGATGAAGTAGATAAAGCATTGACCTCAGATGAGGAGAGGAACAATGCACAGCTGGAATTTGATGATGAACCCAAGGTTATTAACTGGGGGGATTCAGCTTTAGGTGAGAATGAACTTTGAGTAACATAATGGACCTGCTGCATTTTTTGGTACATTTTGTTAAACACTATTTCTGTGCTTTCCTATCAATTATTACAGGCATTGATTGTGATTATGTGCAATATTTAAGCTTCTTTTGGTTGCTTTTTGCTTGTTGTGTTGTTGCTGTGCTTTTTATTATTGTGATTCTCATTAGTTTGTTTTATCGTAGAAGTTCAATAGTAAGAGTTAAGGAAGATAGGCATGTAGCTTAGCACCTACATGTCTATCGCCAGGGAAATGTCTAATCTGTCTACTTAGTAGCCTGGAAACGAACGGTAGACCCTTAGATTTTAATTTAGTTTAATTTTTAGTTTAGTTTAAGTTAGTTTAGAGTAGGTATAAAGATGCCAGTGCCGGGGCCACGCGGAGTACGATCGAGGGTACAGCACTAGGACGCCCATTAAGGGAAGAGCTAAATTTTAGTTTAAGTTAAGTTTAATTGGCTAAGTATAGTTAAAATTTGTAGGCTAGTATAGAGTTAGAGCAAAAAAAAAAAAAA s-eif3f-4 8 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 4A eif3f-s-3 6 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind range range range range myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 4B eif3f-s-n-1 2 15 unspecified role unspecified role prey prey infection infection 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 4D rna_ibv-zcrb1-1 7 16 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined r.25_26delinsag mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TC AG r.11_12delinsct mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain GA CT region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8TBF4:p.Tyr13Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 17:23:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr13Ala' Q8TBF4:p.[Val53Ala;Phe55Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain F A Mon Jun 29 10:07:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val53Ala; Phe55Ala]' Tue Jun 30 17:30:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val53Ala;Phe55Ala]' Q8TBF4:p.[Tyr13Ala;Val53Ala;Phe55Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Y A Mon Jun 29 10:30:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr13Ala; Val53Ala; Phe55Ala]' Tue Jun 30 20:02:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr13Ala;Val53Ala;Phe55Ala]' human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. physical association physical association Fig 1 A, Fig 3, Fig 4 Fig 8 A, Fig 4 rna_ibv-zcrb1-2 7 16 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. 16 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1 B not biotinilated rna_ibv acts as competitor pro_0000338322-1 1 10 unspecified role unspecified role neutral component neutral component purified purified P0C6V3-PRO_0000338322:p.Lys10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:32:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys10Ala' P0C6V3-PRO_0000338322:p.Tyr31Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 18:18:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr31Ala' P0C6V3-PRO_0000338322:p.Lys51Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 15:52:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' P0C6V3-PRO_0000338322:p.Lys90Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:07:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys90Ala' P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 19:30:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:38:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:32:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Ala' P0C6V3-PRO_0000338322:p.Gly98Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 19:26:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 3a, 5a pro_0000338322-2 3 10 unspecified role unspecified role neutral component neutral component purified purified P0C6V3-PRO_0000338322:p.Lys51Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:20:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' P0C6V3-PRO_0000338322:p.Lys90Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 19:23:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys90Ala' P0C6V3-PRO_0000338322:p.Lys10Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 18:11:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys10Ala' P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 16:34:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr31Ala' P0C6V3-PRO_0000338322:p.Ile95Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 15:49:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:10:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figs. 3b, 5a pro_0000338322-pro_0000338322-1 5 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6V3-PRO_0000338322:p.Ile95Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 18:40:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 16:57:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3c rna_ibv-zcrb1-5 7 16 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. 16 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1 B not biotinilated rna_ibv acts as competitor rna_ibv-zcrb1-6 7 16 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. physical association physical association Fig 1 B not biotinilated rna_ibv acts as competitor aibv_rna_3'utr-pro_0000338322-1 4 10 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 19:04:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (middle panel lanes 1 and 2) aibv_rna_antisense_3'utr-pro_0000338322-1 4 10 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 18:23:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (bottom panel lanes 1 and 2) aibv_rna_3'utr-pro_0000338322-2 4 10 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:52:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Ala' P0C6V3-PRO_0000338322:p.Lys90Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:44:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys90Ala' P0C6V3-PRO_0000338322:p.Lys10Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 19:33:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys10Ala' P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 16:36:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr31Ala' P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 19:23:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 16:07:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Ile95Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:49:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Ala' P0C6V3-PRO_0000338322:p.Gly98Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 17:27:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Ala' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (middle panel lanes 3-8), Fig. 5b middle plane aibv_rna_3'utr-pro_0000338322-3 4 10 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Lys10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 16:22:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys10Ala' P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 15:58:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Ala' P0C6V3-PRO_0000338322:p.Gly98Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 18:13:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Ala' P0C6V3-PRO_0000338322:p.Ile95Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 17:59:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 16:23:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr31Ala' P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Only weak interaction was observed Tue Jun 30 19:46:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (middle panel lanes 3-8), Fig. 5b middle panel Only weak interaction was observed for p.Gly98Asp mutant aibv_rna_antisense_3'utr-pro_0000338322-3 4 10 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Gly98Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 19:27:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' P0C6V3-PRO_0000338322:p.Ile95Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 18:27:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Gly98Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 19:28:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Ala' P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 18:36:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Ala' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (bottom panel lanes 3-8) aibv_rna_antisense_3'utr-pro_0000338322-4 4 10 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Ile95Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I A Tue Jun 30 19:05:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Ala' P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:35:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain I N Tue Jun 30 16:19:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile95Asn' P0C6V3-PRO_0000338322:p.Gly98Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Tue Jun 30 19:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Ala' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (bottom panel lanes 3-8) aibv_rna_3'utr-pro_0000338322-4 4 10 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 15:52:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (middle panel lanes 1 and 2) aibv_rna_antisense_3'utr-pro_0000338322-5 4 10 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 16:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly98Asp' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Fig. 4a (bottom panel lanes 1 and 2) DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu X-ray structures of the N- and C-terminal domains of a coronavirus nucleocapsid protein: implications for nucleocapsid formation. X-ray structures of the N- and C-terminal domains of a coronavirus nucleocapsid protein: implications for nucleocapsid formation. Journal of virology 2006 imex curation Jayaram H., Fan H., Bowman BR., Ooi A., Jayaram J., Collisson EW., Lescar J., Prasad BV. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus julien@ntu.edu.sg Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jayaram H., Fan H., Bowman BR., Ooi A., Jayaram J., Collisson EW., Lescar J., Prasad BV. Virus - Publications including interactions involving viral proteins julien@ntu.edu.sg Accepted 2020-JUN-04 AT 16:11 BST AT 16:11 BST by MGT Journal of virology 2006 Only protein-protein interactions imex curation imex curation ncap_ibvb Nucleoprotein N Nucleocapsid protein 6 protein protein ibvb ibvb IBV MASGKAAGKTDAPAPVIKLGGPKPPKVGSSGNASWFQAIKAKKLNTPPPKFEGSGVPDNENIKPSQQHGYWRRQARFKPGKGGRKPVPDAWYFYYTGTGPAADLNWGDTQDGIVWVAAKGADTKSRSNQGTRDPDKFDQYPLRFSDGGPDGNFRWDFIPLNRGRSGRSTAASSAAASRAPSREGSRGRRSDSGDDLIARAAKIIQDQQKKGSRITKAKADEMAHRRYCKRTIPPNYRVDQVFGPRTKGKEGNFGDDKMNEEGIKDGRVTAMLNLVPSSHACLFGSRVTPKLQLDGLHLRFEFTTVVPCDDPQFDNYVKICDQCVDGVGTRPKDDEPKPKSRSSSRPATRGNSPAPRQQRPKKEKKLKKQDDEADKALTSDEERNNAQLEFYDEPKVINWGDAALGENEL 562F39C475DB8872 n-59 1 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 219-349 binding region 219-349 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 2, Tab 1, Tab 2 IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis. Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis. Journal of virology 2015 imex curation Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as tag visualisation tag visualisation Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins nsp10 and nsp16 of the various coronaviruses were incubated with the substrate m7GpppA-RNA in the presence of [3H]AdoMet. Samples were then analyzed by liquid scintillation assays after purification of the 3H-labeled substrates with DEAE-Sephadex chromatography. Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn Accepted 2020-JUL-27 AT 14:47 BST AT 14:47 BST by PPORRAS Journal of virology 2015 Only protein-protein interactions imex curation imex curation The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. Journal of virology 2010 imex curation Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-DEC-02 AT 14:17 GMT AT 14:17 GMT by PPORRAS Journal of virology 2010 Only protein-protein interactions imex curation imex curation The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. Journal of virology 2010 imex curation Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-DEC-02 AT 14:17 GMT AT 14:17 GMT by PPORRAS Journal of virology 2010 Only protein-protein interactions imex curation imex curation The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. Journal of virology 2010 imex curation Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-DEC-02 AT 14:17 GMT AT 14:17 GMT by PPORRAS Journal of virology 2010 Only protein-protein interactions imex curation imex curation The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. Journal of virology 2010 imex curation Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-DEC-02 AT 14:17 GMT AT 14:17 GMT by PPORRAS HeLa cell Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis. Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis. Journal of virology 2015 imex curation Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn 28-Sep-2020: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins Reaction mixtures were spotted onto TLC plates. Radiolabelling was determined using a PhosphorImager. (see ref. 28 pmid:22022266). Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn Accepted 2020-JUL-27 AT 14:47 BST AT 14:47 BST by PPORRAS Journal of virology 2015 Only protein-protein interactions imex curation imex curation The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. The cellular RNA helicase DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. Journal of virology 2010 imex curation Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Accepted 2020-DEC-02 AT 14:17 GMT AT 14:17 GMT by PPORRAS Journal of virology 2010 Only protein-protein interactions imex curation imex curation p0c6y0-pro_0000037368 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein MHV-JHM Murine coronavirus (strain JHM) Murine hepatitis virus AAADWKPGYVMPVLYKYLESPMERVNLWNYGKPITLPTGCMMNVAKYTQLCQYLSTTTLAVPANMRVLHLGAGSDKGVAPGSAVLRQWLPSGSILVDNDMNPFVSDSVASYYGNCITLPFDCQWDLIISDMYDPLTKNIGEYNVSKDGFFTYLCHLIRDKLALGGSVAIKITEFSWNAELYSLMGKFAFWTIFCTNVNASSSEGFLIGINWLNRTRNEIDGKTMHANYLFWRNSTMWNGGAYSLFDMTKFPLKAAGTAVVSLKPDQINDLVLSLIEKGKLLVRDTRKEVFVGDSLVNVK 6882 7180 EE81BAB7014C0235 p0c6y5-pro_0000037400 Putative 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein TGEV Porcine transmissible gastroenteritis coronavirus (strain Purdue) SAEWNPGYSMPTLYKIQRMCLERCNLYNYGAQVKLPDGITTNVVKYTQLCQYLNTTTLCVPHKMRVLHLGAAGASGVAPGSTVLRRWLPDDAILVDNDLRDYVSDADFSVTGDCTSLYIEDKFDLLVSDLYDGSTKSIDGENTSKDGFFTYINGFIKEKLSLGGSVAIKITEFSWNKDLYELIQRFEYWTVFCTSVNTSSSEGFLIGINYLGPYCDKAIVDGNIMHANYIFWRNSTIMALSHNSVLDTPKFKCRCNNALIVNLKEKELNEMVIGLLRKGKLLIRNNGKLLNFGNHFVNTP 6385 6684 0D2EEC28B1F0070F p0c6y0-pro_0000037363 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein MHV-JHM Murine coronavirus (strain JHM) Murine hepatitis virus AGTATEYASNSAILSLCAFSVDPKKTYLDYIQQGGVPVTNCVKMLCDHAGTGMAITIKPEATTNQDSYGGASVCIYCRSRVEHPDVDGLCKLRGKFVQVPLGIKDPVSYVLTHDVCQVCGFWRDGSCSCVGTGSQFQ 4324 4460 915B3D43CB036B21 ddx1_human ATP-dependent RNA helicase DDX1 DDX1 DEAD box protein 1 DEAD box protein retinoblastoma protein protein human Homo sapiens Human MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF C6D0179F83BD8C73 q98vg9-pro_5000140223 Putative 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein FCoV Feline coronavirus (strain FIPV WSU-79/1146) SAEWNPGYSMPTLYKIQRMCLERCNLYNYGAQVRLPDGITTNVVKYTQLCQYLNTTTVCVPHKMRVLHLGAAGASGVAPGSTVLRRWLPDDAILVDNDLRDYVSDADFSVTGDCTSLYIEDKFDLLISDLYDGSTKSIDGENTSKDGFFTYINGFIKEKLSLGGSAAIKITEFSWNKDLYELIQRFEYWTVFCTSVNTSSSEGFLIGINYLGPYCDKAIVDGNIMHANYIFWRNSTIMALSHNSVLDTPKFKCRCNNALIVNLKEKELNEMVVGLLRKGKLLIRNNGKLLNFGNHLVNVP 6410 6709 C3042E7AC26649C3 pppac20-rna-substrate pppAC20 RNA substrate rna ribonucleic acid RNA rna synthetic construct synthetic construct ACCCCCCCCCCCCCCCCCCCC p0c6y1-pro_0000037412 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV SKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSVGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDSLRQPKSSVQ 3784 3928 297665FA9F44AC49 p0c6y1-pro_0000037415 Proofreading exoribonuclease rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV GTGLFKICNKEFSGVHPAYAVTTKALAATYKVNDELAALVNVEAGSEITYKHLISLLGFKMSVNVEGCHNMFITRDEAIRNVRGWVGFDVEATHACGTNIGTNLPFQVGFSTGADFVVTPEGLVDTSIGNNFEPVNSKAPPGEQFNHLRVLFKSAKPWHVIRPRIVQMLADNLCNVSDCVVFVTWCHGLELTTLRYFVKIGKEQVCSCGSRATTFNSHTQAYACWKHCLGFDFVYNPLLVDIQQWGYSGNLQFNHDLHCNVHGHAHVASVDAIMTRCLAINNAFCQDVNWDLTYPHIANEDEVNSSCRYLQRMYLNACVDALKVNVVYDIGNPKGIKCVRRGDVNFRFYDKNPIVRNVKQFEYDYNQHKDKFADGLCMFWNCNVDCYPDNSLVCRYDTRNLSVFNLPGCNGGSLYVNKHAFYTPKFDRISFRNLKAMPFFFYDSSPCETIQVDGVAQDLVSLATKDCITKCNIGGAVCKKHAQMYAEFVTSYNAAVTAGFTFWVTNKLNPYNLWKSFSALQ 5469 5989 5D8D83D56F7F7B5E p0c6x7-pro_0000037322 2'-O-methyltransferase nsp16 ORF1ab polyprotein 1a-1b rep protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus ASQAWQPGVAMPNLYKMQRMLLEKCDLQNYGENAVIPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPRTKHVTKENDSKEGFFTYLCGFIKQKLALGGSIAVKITEHSWNADLYKLMGHFSWWTAFVTNVNASSSEAFLIGANYLGKPKEQIDGYTMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKENQINDMIYSLLEKGRLIIRENNRVVVSSDILVNN 6776 7073 14B6660399D3A801 ddx1_chlae protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to Q92499 k9n7c7-pro_0000422453 2'-O-methyltransferase rep ORF1ab polyprotein 1a-1b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC ASADWKPGHAMPSLFKVQNVNLERCELANYKQSIPMPRGVHMNIAKYMQLCQYLNTCTLAVPANMRVIHFGAGSDKGIAPGTSVLRQWLPTDAIIIDNDLNEFVSDADITLFGDCVTVRVGQQVDLVISDMYDPTTKNVTGSNESKALFFTYLCNLINNNLALGGSVAIKITEHSWSVELYELMGKFAWWTVFCTNANASSSEGFLLGINYLGTIKENIDGGAMHANYIFWRNSTPMNLSTYSLFDLSKFQLKLKGTPVLQLKESQINELVISLLSQGKLLIRDNDTLSVSTDVLVNTYRKLR 6776 7078 3C6BDE237B9463F9 p0c6y1-pro_0000037417 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV SAWTCGYNMPELYKVQNCVMEPCNIPNYGVGITLPSGILMNVAKYTQLCQYLSKTTICVPHNMRVMHFGAGSDKGVAPGSTVLKQWLPEGTLLVDNDIVDYVSDAHVSVLSDCNKYNTEHKFDLVISDMYTDNDSKRKHEGVIANNGNDDVFIYLSSFLRNNLALGGSFAVKVTETSWHEVLYDIAQDCAWWTMFCTAVNASSSEAFLIGVNYLGASEKVKVSGKTLHANYIFWRNCNYLQTSAYSIFDVAKFDLRLKATPVVNLKTEQKTDLVFNLIKCGKLLVRDVGNTSFTSDSFVCTM 6328 6629 15ECCB26B9D5423D pppac20_rna_substrate-rep-5 1 19 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6Y1-PRO_0000037412:p.Lys98Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A P0C6Y1-PRO_0000037412:p.Phe101Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain F A ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 1G, 2, 4F, 5A IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. The nsp16 of IBV (Gammacoronavirus) was activated and stimulated y only by its cognate nsp10. Current Uniprot P0C6Y1 sequence numbering for IBV nsp10 differs from that given in figure 3A. pppac20_rna_substrate-rep-12 6 19 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 1G IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. pppac20_rna_substrate-rep-21 1 9 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 4A Weak interaction;TGEV (Porcine transmissible gastroenteritis coronavirus) nsp16 protein and IBV nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronavirus but less efficiently activated when using the nsp10 of Betacoronavirus and Gammacoronavirus. pppac20_rna_substrate-rep-26 1 12 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) methylation methylation reaction methylation 4B FCoV (feline coronavirus) nsp16 protein and IBV nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-29 1 8 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 4C MHV (mouse hepatitis virus) 16 protein and IBV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-34 1 16 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 4D SARS-CoV 16 proteins and IBV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-37 1 18 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 4E MERS-CoV 16 protein and IBV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-43 1 10 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 19 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P0C6Y1-PRO_0000037412:p.Lys98Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain K A P0C6Y1-PRO_0000037412:p.Phe101Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain F A ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro methylation methylation reaction methylation 5B IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. The nsp16 of IBV (Gammacoronavirus) was activated and stimulated y only by its cognate nsp10. Current Uniprot P0C6Y1 sequence numbering for IBV nsp10 differs from that given in figure 3A. rep-ddx1-1 5 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1A rep-ddx1-2 2 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B/C rep-ddx1-3 2 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1C rep-ddx1-4 4 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1D self-activation was observed in all yeast colonies transformed with DDX1Δ2 (aas 536-631) rep-ddx1_chlae-1 7 17 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 15 unspecified role unspecified role bait bait infection infection physical association physical association 3 ddx1-rep-2 3 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q92499:p.[Asp370_Glu371delinsAlaAla;Asp373Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain DE AA certain certain sequence position certain certain certain sequence position certain D A Thu Sep 17 11:21:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'Q92499:p.[Asp370Ala;Glu371Ala;Asp373Ala]' 15 unspecified role unspecified role neutral component neutral component infection infection colocalization colocalization 8