IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Molecular Biomedicine 2020 imex curation Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn Accepted 2021-MAR-31 AT 11:27 BST AT 11:27 BST by ORCHARD Molecular Biomedicine 2020 Only protein-protein interactions imex curation imex curation Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Molecular Biomedicine 2020 imex curation Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn Accepted 2021-MAR-31 AT 11:27 BST AT 11:27 BST by ORCHARD Molecular Biomedicine 2020 Only protein-protein interactions imex curation imex curation Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Structural characterization of the C-terminal domain of SARS-CoV-2 nucleocapsid protein Molecular Biomedicine 2020 imex curation Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight by staining molecular weight estimation by staining weight by staining Zhou R. et al., Zhou R. et al. Virus - Publications including interactions involving viral proteins PMCID: PMC7406681 There is NO Pubmed identifier I can find; this IS peer reviewed. probably a new journal? https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406681/ leijian@scu.edu.cn Accepted 2021-MAR-31 AT 11:27 BST AT 11:27 BST by ORCHARD Molecular Biomedicine 2020 Only protein-protein interactions imex curation imex curation 17mer_dsdna ds dna double stranded deoxyribonucleic acid ds DNA ds dna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV TGTTCTCTAAACGAACT genomic_rna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV rna mimic dna ss dna single stranded deoxyribonucleic acid ss DNA ss dna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV TGTTCTCTAAACGAACT ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA n-80 2 7 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Table 1 n-81 1 7 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figure 2G RHS, Supp. figure 1 any possible PLPro interaction not entered as no details are given genomic_rna-n-21 3 5 unspecified role unspecified role neutral component neutral component 5' utr region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D LHS The amount of the free 17-mer RNA decreased with the addition of more N-CTD protein. n-rna mimic dna-1 3 6 unspecified role unspecified role neutral component neutral component 5' utr region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D middle The amount of the ssDNA decreased with the addition of more N-CTD protein. 17mer_dsdna-n-1 3 4 unspecified role unspecified role neutral component neutral component 5' utr region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3D RHS The amount of the dsDNA decreased with the addition of more N-CTD protein.