IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Heparin interacts with the main protease of SARS-CoV-2 and inhibits its activity. Heparin interacts with the main protease of SARS-CoV-2 and inhibits its activity. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 imex curation Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins FRET based assay:On cleavage of the peptide by the protease, the quencher (DNP) and fluorophore (MCA) are separated resulting in a large increase in MCA fluorescence. 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Accepted 2021-DEC-16 AT 11:06 GMT AT 11:06 GMT by KALPANAP Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 Only protein-protein interactions imex curation imex curation Heparin interacts with the main protease of SARS-CoV-2 and inhibits its activity. Heparin interacts with the main protease of SARS-CoV-2 and inhibits its activity. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 imex curation Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Only protein-protein interactions imex curation in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Li J., Zhang Y., Pang H., Li SJ. Virus - Publications including interactions involving viral proteins Fluorescence quenching was carried out at various temperatures. 1120190087@mail.nankai.edu.cn, shujieli@nankai.edu.cn Accepted 2021-DEC-16 AT 11:06 GMT AT 11:06 GMT by KALPANAP Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 mca-avlqsgfr-lys(dnp)-lys-nh2 MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) AVLQSGFR heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gc-376 GC-376 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) heparin-rep-1 2 3 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified pig-intestinal mucosa pig Pig intestinal mucosa direct interaction direct interaction 2 GST tag has been cleaved according to methods and M.Wt in figure 1A. Maybe all Kd values should be removed? I do not see any difference in methods to explain the difference in results between Table 1 and figure 2?? Please let me know if you do, and I will correct - if not, I will ask the authors, in the improbable expectation that they will reply...(China!) In the text the Dissociation constants are given as 1.66 uM at 25 deg.C and and 3.16 at 35 deg.C -average 2.41 uM. However results in Table 1 give X10 higher values for Kd at these temperatures. A higher concentration of Sodium chloride did not significantly influence the interaction of heparin with Mpro. heparin-rep-2 1 4 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 5 inhibitor inhibitor neutral component neutral component purified purified pig-intestinal mucosa pig Pig intestinal mucosa direct interaction direct interaction 3 C/D, 4 A-D heparin-mca_avlqsgfr_lys(dnp)_lys_nh2-1 1 4 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 A/B, 4 gc_376-mca_avlqsgfr_lys(dnp)_lys_nh2-1 1 4 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 6 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4 E/F