IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. FEBS letters 2021 imex curation Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. As there is no mention of any (SILAC) high confidence dataset - nor in any of the references that could be accessed - only MS results entered for interactions found in other experiments have been captured. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Accepted 2022-JAN-12 AT 16:47 GMT AT 16:47 GMT by SIMONA FEBS letters 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. FEBS letters 2021 imex curation Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa anti tag western anti tag western blot anti tag western Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Accepted 2022-JAN-12 AT 16:47 GMT AT 16:47 GMT by SIMONA FEBS letters 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. FEBS letters 2021 imex curation Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Accepted 2022-JAN-12 AT 16:47 GMT AT 16:47 GMT by SIMONA FEBS letters 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. FEBS letters 2021 imex curation Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Accepted 2022-JAN-12 AT 16:47 GMT AT 16:47 GMT by SIMONA FEBS letters 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets. FEBS letters 2021 imex curation Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. syam@mail.ubc.ca Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Somasekharan SP., Gleave M., Somasekharan SP., Gleave M. 1] there is NO mention of any (SILAC) high confidence dataset - not in any of the references that I could access: thus only MS results entered for interactions found in other experiments. Table S1 contains lots of CRAPOME proteins. 2] The description/captions for the Supp. figure legends are very short and not informative- but I cannot find any better ones. I have not added any interactions from figures 6D/Supp.6 and 8 as not sure what they show. Also for the other later Supp. figures. 3] All "cells" are A549 as no others appear to have been used (apart from E.coli). It is usually fairly obvious which they mean - i think- but please check. Liquid–liquid phase separation assay (LLPS). syam@mail.ubc.ca Accepted 2022-JAN-12 AT 16:47 GMT AT 16:47 GMT by SIMONA FEBS letters 2021 Only protein-protein interactions imex curation imex curation ubp10_human Ubiquitin carboxyl-terminal hydrolase 10 USP10 KIAA0190 Ubiquitin-specific-processing protease 10 Deubiquitinating enzyme 10 Ubiquitin thioesterase 10 protein protein human Homo sapiens Human MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL E6BA77E2B5CE2B3F mitoxantrone mitoxantrone mitoxantrona 1,4-DIHYDROXY-5,8-BIS({2-[(2-HYDROXYETHYL)AMINO]ETHYL}AMINO)-9,10-ANTHRACENEDIONE mitoxantrone Mitoxantrone mitoxantronum 1,4-dihydroxy-5,8-bis({2-[(2-hydroxyethyl)amino]ethyl}amino)anthracene-9,10-dione small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA genomic_rna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ 0F9429D78E0C7F59 e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC DIP protein P19525 original sequence version: 164 DIP protein P19525-1 original sequence version: 177 DIP protein P19525-1 original sequence version: 178 DIP protein P19525-1 original sequence version: 170 DIP protein P19525 original sequence version: 153 815AD83ACAB45DA3 ubp32_human Ubiquitin carboxyl-terminal hydrolase 32 USP32 USP10 Ubiquitin-specific-processing protease 32 Deubiquitinating enzyme 32 Renal carcinoma antigen NY-REN-60 Ubiquitin thioesterase 32 protein protein human Homo sapiens Human MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMGQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRNWLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLEVWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFSSSPSTNEMFTLTTNGDLPRPIFIPNGMPNTVVPCGTEKNFTNGMVNGHMPSLPDSPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPALCQHKPLTPQGDELSEPRILAREVKKVDAQSSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSSKNSSPNSSPRTLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRGHVLGGSQPELVTPQDHEVALANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREDTRIKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ DIP protein Q8NFA0 original sequence version: 145 A621F764B76321E3 g3bp2_human Ras GTPase-activating protein-binding protein 2 GAP SH3 domain-binding protein 2 G3BP2 KIAA0660 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR DIP protein Q9UN86 original sequence version: 147 74B8EA43C0560229 ythd3_human YTH domain-containing family protein 3 YTHDF3 protein protein human Homo sapiens Human MSATSVDQRPKGQGNKVSVQNGSIHQKDAVNDDDFEPYLSSQTNQSNSYPPMSDPYMPSYYAPSIGFPYSLGEAAWSTAGDQPMPYLTTYGQMSNGEHHYIPDGVFSQPGALGNTPPFLGQHGFNFFPGNADFSTWGTSGSQGQSTQSSAYSSSYGYPPSSLGRAITDGQAGFGNDTLSKVPGISSIEQGMTGLKIGGDLTAAVTKTVGTALSSSGMTSIATNSVPPVSSAAPKPTSWAAIARKPAKPQPKLKPKGNVGIGGSAVPPPPIKHNMNIGTWDEKGSVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQNRWVAPRNRGAGFNQNNGAGSENFGLGVVPVSASPSSVEVHPVLEKLKAINNYNPKDFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDAAYRSLNGKGPLYLLFSVNGSGHFCGVAEMKSVVDYNAYAGVWSQDKWKGKFEVKWIFVKDVPNNQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATFKHTTSIFDDFAHYEKRQEEEEAMRRERNRNKQ C7021FE48DEA441E n-116 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 4A n-eif2ak2-4 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 2, Tables S1 and 2 g3bp1-n-23 3 8 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization 5A, 7C/D, 9 A/B, Supp. figure 3 arsenite treatment heat treatment Treatment with RNase A Mitoxantrone -CHEBI:50729 g3bp1-n-21 3 8 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization 5A, Supp. figure 3 arsenite treatment heat stress g3bp1-n-22 5 10 unspecified role unspecified role neutral component neutral component partially purified partially purified region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component partially purified partially purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) colocalization colocalization 6C genomic_rna-n-35 2 9 unspecified role unspecified role bait bait 5’UTR leader sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NC_045512.2:191-214 region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey partially purified partially purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 8 B/C genomic_rna-n-36 2 9 competitor competitor competitor unspecified role unspecified role partially purified partially purified 5’UTR leader sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NC_045512.2:191-214 ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait 5’UTR leader sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NC_045512.2:191-214 region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey partially purified partially purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 8 B genomic_rna-n-37 2 7 inhibitor inhibitor unspecified role unspecified role partially purified partially purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait 5’UTR leader sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NC_045512.2:191-214 region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey partially purified partially purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 8 C n-n-86 5 7 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association 9 C/D GFP-NCAP granules (aggregates) isolated from arsenite- or heat-stressed cells. n-n-87 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association 7B, 9 C/D GFP-NCAP granules (aggregates) isolated from arsenite- or heat-stressed cells. RNAse A g3bp1-n-24 3 8 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp. fig 2 Cells were untreated.