IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Susceptibilities of human ACE2 genetic variants in coronavirus infection. Susceptibilities of human ACE2 genetic variants in coronavirus infection. Journal of virology 2021 imex curation Ren W., Zhu Y., Lan J., Chen H., Wang Y., Shi H., Feng F., Chen DY., Close B., Zhao X., Wu J., Tian B., Yuan Z., Zhou D., Saeed M., Wang X., Zhang R., Ding Q. Coronavirus - Interactions investigated in the context of Coronavirus Virus - Publications including interactions involving viral proteins qding@tsinghua.edu.cn Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ren W., Zhu Y., Lan J., Chen H., Wang Y., Shi H., Feng F., Chen DY., Close B., Zhao X., Wu J., Tian B., Yuan Z., Zhou D., Saeed M., Wang X., Zhang R., Ding Q. Coronavirus - Interactions investigated in the context of Coronavirus Mutants:arbitrarily for Kd values I have taken +/- 50nM as limits for "no effect" for SARS2; +/-125nM for SARS; +/-145 for NL63. qding@tsinghua.edu.cn Accepted 2021-DEC-02 AT 14:47 GMT AT 14:47 GMT by ORCHARD Journal of virology 2021 Only protein-protein interactions imex curation imex curation spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_cvhnl Spike glycoprotein S E2 Peplomer protein 2 protein protein cvhnl Human coronavirus NL63 HCoV-NL63 MKLFLILLVLPLASCFFTCNSNANLSMLQLGVPDNSSTIVTGLLPTHWFCANQSTSVYSANGFFYIDVGNHRSAFALHTGYYDANQYYIYVTNEIGLNASVTLKICKFSRNTTFDFLSNASSSFDCIVNLLFTEQLGAPLGITISGETVRLHLYNVTRTFYVPAAYKLTKLSVKCYFNYSCVFSVVNATVTVNVTTHNGRVVNYTVCDDCNGYTDNIFSVQQDGRIPNGFPFNNWFLLTNGSTLVDGVSRLYQPLRLTCLWPVPGLKSSTGFVYFNATGSDVNCNGYQHNSVVDVMRYNLNFSANSLDNLKSGVIVFKTLQYDVLFYCSNSSSGVLDTTIPFGPSSQPYYCFINSTINTTHVSTFVGILPPTVREIVVARTGQFYINGFKYFDLGFIEAVNFNVTTASATDFWTVAFATFVDVLVNVSATNIQNLLYCDSPFEKLQCEHLQFGLQDGFYSANFLDDNVLPETYVALPIYYQHTDINFTATASFGGSCYVCKPHQVNISLNGNTSVCVRTSHFSIRYIYNRVKSGSPGDSSWHIYLKSGTCPFSFSKLNNFQKFKTICFSTVEVPGSCNFPLEATWHYTSYTIVGALYVTWSEGNSITGVPYPVSGIREFSNLVLNNCTKYNIYDYVGTGIIRSSNQSLAGGITYVSNSGNLLGFKNVSTGNIFIVTPCNQPDQVAVYQQSIIGAMTAVNESRYGLQNLLQLPNFYYVSNGGNNCTTAVMTYSNFGICADGSLIPVRPRNSSDNGISAIITANLSIPSNWTTSVQVEYLQITSTPIVVDCATYVCNGNPRCKNLLKQYTSACKTIEDALRLSAHLETNDVSSMLTFDSNAFSLANVTSFGDYNLSSVLPQRNIRSSRIAGRSALEDLLFSKVVTSGLGTVDVDYKSCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIGGMVLGGLTSAAAIPFSLALQARLNYVALQTDVLQENQKILAASFNKAINNIVASFSSVNDAITQTAEAIHTVTIALNKIQDVVNQQGSALNHLTSQLRHNFQAISNSIQAIYDRLDSIQADQQVDRLITGRLAALNAFVSQVLNKYTEVRGSRRLAQQKINECVKSQSNRYGFCGNGTHIFSIVNSAPDGLLFLHTVLLPTDYKNVKAWSGICVDGIYGYVLRQPNLVLYSDNGVFRVTSRVMFQPRLPVLSDFVQIYNCNVTFVNISRVELHTVIPDYVDVNKTLQEFAQNLPKYVKPNFDLTPFNLTYLNLSSELKQLEAKTASLFQTTVELQGLIDQINSTYVDLKLLNRFENYIKWPWWVWLIISVVFVVLLSLLVFCCLSTGCCGCCNCLTSSMRGCCDCGSTKLPYYEFEKVHVQ DIP protein Q6Q1S2 original sequence version: 49 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT s-ace2-126 1 3 unspecified role unspecified role prey prey purified purified Q9BYF1:p.Gly352Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G V Thu Jan 06 19:33:57 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly352Val' Q9BYF1:p.Asp355Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N Thu Jan 06 18:59:48 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp355Asn' partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.Ser19Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S P Thu Jan 06 20:04:40 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser19Pro' Q9BYF1:p.Lys26Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Thu Jan 06 18:42:00 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Glu' Q9BYF1:p.Lys26Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Thu Jan 06 20:09:42 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Arg' Q9BYF1:p.Thr27Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T A Thu Jan 06 18:57:46 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Ala' Q9BYF1:p.Glu35Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 19:59:01 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu35Lys' Q9BYF1:p.Glu37Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 19:01:07 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Lys' Q9BYF1:p.Phe40Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F L Thu Jan 06 18:28:41 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe40Leu' Q9BYF1:p.Met82Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M I Thu Jan 06 18:27:30 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met82Ile' Q9BYF1:p.Thr92Ile mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T I Thu Jan 06 19:09:09 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr92Ile' Q9BYF1:p.Glu329Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E G Thu Jan 06 18:49:50 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu329Gly' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction 3C, number 1 s-ace2-127 1 3 unspecified role unspecified role prey prey purified purified Q9BYF1:p.Gly352Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain G V Thu Jan 06 18:49:42 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly352Val' Q9BYF1:p.Asp355Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N Thu Jan 06 19:46:41 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp355Asn' partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.Ser19Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S P Thu Jan 06 18:53:40 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser19Pro' Q9BYF1:p.Lys26Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Thu Jan 06 20:08:22 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Glu' Q9BYF1:p.Lys26Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K R Thu Jan 06 18:55:07 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Arg' Q9BYF1:p.Thr27Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Thu Jan 06 18:41:08 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Ala' Q9BYF1:p.Glu35Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 19:11:13 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu35Lys' Q9BYF1:p.Glu37Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 19:31:40 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Lys' Q9BYF1:p.Phe40Leu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F L Thu Jan 06 18:54:21 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe40Leu' Q9BYF1:p.Met82Ile mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M I Thu Jan 06 18:39:55 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met82Ile' Q9BYF1:p.Thr92Ile mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T I Thu Jan 06 18:50:57 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr92Ile' Q9BYF1:p.Glu329Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E G Thu Jan 06 19:19:57 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu329Gly' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction 3C, number 2 s-ace2-128 1 3 unspecified role unspecified role prey prey purified purified Q9BYF1:p.Gly352Val mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G V Thu Jan 06 19:53:00 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly352Val' Q9BYF1:p.Asp355Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D N Thu Jan 06 20:27:02 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp355Asn' partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.Ser19Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S P Thu Jan 06 20:05:44 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser19Pro' Q9BYF1:p.Lys26Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Thu Jan 06 19:58:27 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Glu' Q9BYF1:p.Lys26Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Thu Jan 06 19:15:27 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys26Arg' Q9BYF1:p.Thr27Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Thu Jan 06 19:41:56 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr27Ala' Q9BYF1:p.Glu35Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 19:58:47 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu35Lys' Q9BYF1:p.Glu37Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 20:11:47 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Lys' Q9BYF1:p.Phe40Leu mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain F L Thu Jan 06 20:16:29 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe40Leu' Q9BYF1:p.Met82Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M I Thu Jan 06 19:53:47 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met82Ile' Q9BYF1:p.Thr92Ile mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T I Thu Jan 06 19:09:41 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr92Ile' Q9BYF1:p.Glu329Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E G Thu Jan 06 19:46:42 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu329Gly' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GP67 signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction 3C, number 3