DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Genetic prion disease-related mutation E196K displays a novel amyloid fibril structure revealed by cryo-EM. Genetic prion disease-related mutation E196K displays a novel amyloid fibril structure revealed by cryo-EM. Science advances 2021 imex curation Wang LQ., Zhao K., Yuan HY., Li XN., Dang HB., Ma Y., Wang Q., Wang C., Sun Y., Chen J., Li D., Zhang D., Yin P., Liu C., Liang Y. liangyi@whu.edu.cn Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Wang LQ., Zhao K., Yuan HY., Li XN., Dang HB., Ma Y., Wang Q., Wang C., Sun Y., Chen J., Li D., Zhang D., Yin P., Liu C., Liang Y. liangyi@whu.edu.cn Accepted 2021-DEC-02 AT 10:15 GMT AT 10:15 GMT by MGT Science advances 2021 Only protein-protein interactions imex curation imex curation prio_human Major prion protein PrP27-30 PrP33-35C ASCR PRNP PRIP PRP ALTPRP protein protein human Homo sapiens Human MANLGCWMLVLFVATWSDLGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHSQWNKPSKPKTNMKHMAGAAAAGAVVGGLGGYMLGSAMSRPIIHFGSDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCITQYERESQAYYQRGSSMVLFSSPPVILLISFLIFLIVG DIP protein P04156 original sequence version: 125 DIP protein P04156 original sequence version: 122 DIP protein P04156 original sequence version: 147 DIP protein P04156 original sequence version: 141 DIP protein P04156 original sequence version: 166 DIP protein P04156-1 original sequence version: 166 43DB596BAAA66484 prnp-41 1 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified P04156:p.Glu196Lys p.E196K mutation mutation certain certain sequence position certain certain certain sequence position certain E K Thu Jan 06 20:18:34 GMT 2022 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu196Lys' binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Table 1 The PrP protein may still have N terminal His, thrombin and S Tags from the pET-30a (+) vector, as the tags are not stated to have been removed.