IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Antiviral research 2021 imex curation Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins Chromatin - Epigenetic interactions resulting in chromatin modulation calonso@inia.es Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins calonso@inia.es Accepted 2021-SEP-14 AT 08:22 BST AT 08:22 BST by KALPANAP Antiviral research 2021 Only protein-protein interactions imex curation imex curation Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Antiviral research 2021 imex curation Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins Chromatin - Epigenetic interactions resulting in chromatin modulation calonso@inia.es Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins calonso@inia.es Accepted 2021-SEP-14 AT 08:22 BST AT 08:22 BST by KALPANAP Antiviral research 2021 Only protein-protein interactions imex curation imex curation Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Identification of Niemann-Pick C1 protein as a potential novel SARS-CoV-2 intracellular target. Antiviral research 2021 imex curation Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins Chromatin - Epigenetic interactions resulting in chromatin modulation calonso@inia.es Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Garcia-Dorival I., Cuesta-Geijo MÁ., Barrado-Gil L., Galindo I., Garaigorta U., Urquiza J., Puerto AD., Campillo NE., Martínez A., Gastaminza P., Gil C., Alonso C. Virus - Publications including interactions involving viral proteins calonso@inia.es Accepted 2021-SEP-14 AT 08:22 BST AT 08:22 BST by KALPANAP Antiviral research 2021 Only protein-protein interactions imex curation imex curation ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA npc1_human NPC intracellular cholesterol transporter 1 NPC1 Niemann-Pick C1 protein protein protein human Homo sapiens Human MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF DIP protein O15118 original sequence version: 157 DA1523E09822E5C7 n-npc1-1 2 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1C n-npc1-2 1 5 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1D n-npc1-3 3 5 unspecified role unspecified role prey prey partially purified partially purified human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. physical association physical association Supp. figure 4 I have entered the expression system as Trini-hi5, as purification details are given for this system - unlike for the Sf9 system, which is also mentioned.