IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Dear checker:The purification details for figures 5A/B are as clear as mud. There are NO purification details for the individual proteins, only the co-expressed complex. So it is unclear if the GST tag (Nsp3.1) has been removed in figure 5B, although I would think so. The text at the top of figure 5, and body of text, states "using purified proteins"; but I am not convinced and have entered 5A as "physical assoc." and "partially purified". Same for 5B. Let me know if you disagree or alter yourself!. panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins There are 53 positives here -counted several times - I do not know what the "48 interactions were detected" refers to ? Mammalian two-hybrid assay; Vectors -Bait (DNA binding Domain) uses plasmid "pM" and Prey (DNA Activation domain) plasmid VP16 with Luciferase Reporter. panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication. Cell & bioscience 2021 imex curation Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Dear checker:The purification details for figures 5A/B are as clear as mud. There are NO purification details for the individual proteins, only the co-expressed complex. So it is unclear if the GST tag (Nsp3.1) has been removed in figure 5B, although I would think so. The text at the top of figure 5, and body of text, states "using purified proteins"; but I am not convinced and have entered 5A as "physical assoc." and "partially purified". Same for 5B. Let me know if you disagree or alter yourself!. panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Accepted 2021-AUG-30 AT 07:20 BST AT 07:20 BST by KALPANAP Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449630 Helicase nsp13 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ 5325 5925 ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA p0dtc2-pro_0000449648 Spike protein S2 S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 686 1273 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT a0a7g5x2w6_sars2 ORF10 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT AE48AE9E0598073C p0dtd1-pro_0000449620 Non-structural protein 2 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG 181 818 p0dtd1-pro_0000449625 Non-structural protein 7 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQ 3860 3942 p0dtd1-pro_0000449628 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ 4254 4392 p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 p0dtd1-pro_0000449627 Non-structural protein 9 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV NNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ 4141 4253 p0dtd1-pro_0000449633 2'-O-methyltransferase nsp16 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN 6799 7096 ap3a_sars2 ORF3a protein Accessory protein 3a Protein U274 Protein X1 3a Protein 3a protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL vme1_sars2 Membrane protein E1 glycoprotein Matrix glycoprotein Membrane glycoprotein M protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ ns8_sars2 ORF8 protein Non-structural protein 8 8 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFI 912AD9E147A64907 ns7a_sars2 ORF7a protein Accessory protein 7a Protein U122 Protein X4 7a protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE ns7b_sars2 ORF7b protein Accessory protein 7b 7b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA p0dtd1-pro_0000449621 Papain-like protease nsp3 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 p0dtd1-pro_0000449619 Host translation inhibitor nsp1 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG 1 180 p0dtd1-pro_0000449631 Proofreading exoribonuclease nsp14 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQ 5926 6452 p0dtd1-pro_0000449632 Uridylate-specific endoribonuclease nsp15 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ 6453 6798 p0dtd1-pro_0000449629 RNA-directed RNA polymerase nsp12 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ 4393 5324 p0dtd1-pro_0000449624 Non-structural protein 6 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ 3570 3859 p0dtd1-pro_0000449622 Non-structural protein 4 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV KIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQ 2764 3263 ns6_sars2 ORF6 protein Accessory protein 6 Protein X3 6 Non-structural protein 6 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEID vemp_sars2 Envelope small membrane protein E 4 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV p0dtd1-pro_0000449626 Non-structural protein 8 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ 3943 4140 rep-rep-224 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 1 Nsp1;Nsp3.1 rep-rep-223 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 2 Nsp1;Nsp3.2 rep-rep-225 3 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 3 Nsp1;Nsp12 rep-rep-226 3 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 4 Nsp1;Nsp14 rep-s-7 3 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 5 Nsp1;S2. S2 sequence deduced from nucleotide numbering in Table 1. rep-ns7a_sars2-2 3 21 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 6 Nsp1;Orf 7a. rep-ns7b_sars2-1 3 22 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 7 Nsp1;Orf 7b. rep-ns7b_sars2-2 3 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 8 Nsp1;Orf8. rep-n-2 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 24 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 9 Nsp1;N. rep-rep-227 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 10 Nsp2;Nsp1. rep-rep-228 3 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 11 Nsp2;Nsp14. rep-rep-229 3 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 12 Nsp3.1;Nsp1. rep-rep-230 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 13 Nsp3.1;Nsp3.1. rep-rep-231 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.3 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 14 Nsp3.1;Nsp3.3. rep-rep-232 3 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 15 Nsp3.1;Nsp14. rep-n-3 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1 A/B, Number 16 Nsp3.1;N. rep-rep-233 3 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 17 Nsp3.2;Nsp12. rep-rep-234 3 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 29 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 18 Nsp4;Nsp1. rep-e-2 3 31 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 29 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 19 Nsp4;E. rep-ns6_sars2-4 3 30 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 29 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 20 Nsp4;Orf6. rep-rep-235 3 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 21 Nsp5;Nsp5. rep-rep-236 3 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 22 Nsp5;Nsp12. rep-rep-237 3 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 28 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 23 Nsp6;Nsp1. rep-rep-238 3 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 13 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 24 Nsp7;Nsp1. rep-rep-239 3 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 25 Nsp9;Nsp1. rep-ns8_sars2-1 3 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 16 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 26 Nsp9;Orf8. rep-rep-240 3 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 27 Nsp10;Nsp1. rep-rep-241 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 28 Nsp10;Nsp3.1. rep-rep-242 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 29 Nsp10;Nsp3.2. rep-rep-243 3 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 30 Nsp10;Nsp12. rep-rep-244 3 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 31 Nsp10;Nsp14. rep-rep-245 3 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 32 Nsp10;Nsp16. rep-e-3 3 31 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 33 Nsp10;E. rep-ns6_sars2-5 3 30 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 34 Nsp10;Orf6. rep-ns8_sars2-2 3 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 35 Nsp10;Orf8. rep-n-4 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 36 Nsp10;N. rep-rep-246 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 27 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 37 Nsp12;Nsp3.2. rep-rep-247 3 32 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 27 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 38 Nsp12;Nsp8. rep-rep-248 3 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 39 Nsp13;Nsp3.2. rep-rep-249 3 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 40 Nsp13;Nsp14. rep-rep-250 3 25 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 41 Nsp14;Nsp10. rep-rep-251 3 26 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 26 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 42 Nsp15;Nsp15. rep-rep-252 3 17 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 14 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 43 Nsp16;Nsp10. rep-rep-253 3 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 44 S1;Nsp1. S1 sequence deduced from nucleotide numbering in Table 1. ap3a_sars2-rep-2 3 18 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 45 Orf3:Nsp3.1 Identity of Orf3 found from Table 1 stated position in polyprotein. ap3a_sars2-rep-3 3 19 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 24 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 46 M:Nsp1 vme1_sars2-rep-1 3 19 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 47 M:Nsp12 vme1_sars2-ns6_sars2-1 3 19 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 30 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 48 M:Orf6. n-rep-2 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 49 N:Nsp3.1. n-n-85 3 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 50 N:N. orf10-rep-3 3 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Number 51 Orf10:Nsp3.2 There is no Uniprot reviewed entry for Orf10;this may be because that the genome sequence currently annotated as orf10 may not have a protein coding function in SARS-CoV-2. A random Trembl entry has been used here, based on the nucleotide sequence numbers given in Table 1. orf10-rep-4 3 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 27 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association 1B, Number 52 Orf10:Nsp12. There is no Uniprot reviewed entry for Orf10;this may be because that the genome sequence currently annotated as orf10 may not have a protein coding function in SARS-CoV-2. A random Trembl entry has been used here, based on the nucleotide sequence numbers given in Table 1. rep-ns8_sars2-3 3 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association 1B, Number 53 Nsp9;Orf8. ns8_sars2-ns8_sars2-1 4 20 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2A ns8_sars2-ns8_sars2-2 4 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2B rep-rep-254 4 26 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2C rep-rep-255 4 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2D rep-rep-256 4 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2E rep-rep-257 4 14 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2F rep-n-5 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2G rep-rep-258 4 32 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2H rep-n-6 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NTD sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3 B/C The N-terminal number for the "N" protein has been taken from Uniprot, as it does not appear to have been stated. Compared with N, NTD interacted with Nsp3.1 in a stronger manner. n-101 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NTD sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 23 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 3 D-F The N-terminal number for the "N" protein has been taken from Uniprot, as it does not appear to have been stated. n-102 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3 G/H n-103 4 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4 B/C/I n-104 4 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4 G/H n-rep-3 5 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 4 D-F Only aa 1-235 of Nsp3.1 plays an indispensable role in the interaction between Nsp3.1 and N. rep-n-7 6 8 unspecified role unspecified role prey prey partially purified partially purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait partially purified partially purified 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 5A rep-n-8 1 8 unspecified role unspecified role prey prey partially purified partially purified possible tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 23 unspecified role unspecified role bait bait partially purified partially purified region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined possible tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 5B rep-n-9 2 8 unspecified role unspecified role neutral component neutral component purified purified NTD region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role neutral component neutral component purified purified 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5C-H not entered as "Direct" interaction as there are several extra faint bands present on gels, and possible oligomers are mentioned. n-rep-4 4 23 competitor competitor competitor prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 6B Both Preys have an HA tag so I am not sure that the 1-235 construct is a "competitor".