IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. PLoS biology 2021 imex curation Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli antibody detection identification by antibody antibody detection Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Accepted 2021-MAY-31 AT 12:08 BST AT 12:08 BST by KALPANAP PLoS biology 2021 Only protein-protein interactions imex curation imex curation Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. PLoS biology 2021 imex curation Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Accepted 2021-MAY-31 AT 12:08 BST AT 12:08 BST by KALPANAP PLoS biology 2021 Only protein-protein interactions imex curation imex curation Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. Cryo-electron microscopy structures of the N501Y SARS-CoV-2 spike protein in complex with ACE2 and 2 potent neutralizing antibodies. PLoS biology 2021 imex curation Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S., Zhu X., Mannar D., Srivastava SS., Berezuk AM., Demers JP., Saville JW., Leopold K., Li W., Dimitrov DS., Tuttle KS., Zhou S., Chittori S., Subramaniam S. Virus - Publications including interactions involving viral proteins Addgene plasmid #154754 (Spike) does not mention a "C-terminal foldon trimerization motif" but the originating reference (pmid:32703906) does have this tag, as do the PDB entries. sriram.subramaniam@ubc.ca Accepted 2021-MAY-31 AT 12:08 BST AT 12:08 BST by KALPANAP PLoS biology 2021 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2-s-94 2 4 unspecified role unspecified role neutral component neutral component purified purified partial_ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role neutral component neutral component purified purified T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HRV3C protease cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Asn501Tyr;Arg682_Arg685delinsGlySerAlaSer;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain RRAR GSAS certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain KV PP Fri Sep 18 23:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.Lys986_Val987delinsProPro [986-987]' Mon Jun 28 17:48:49 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Asn501Tyr;Lys986_Val987delinsProPro;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Arg682_Arg683delinsGlySer;Arg685Ser]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Table 1 ace2-s-92 2 4 unspecified role unspecified role neutral component neutral component purified purified partial_ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role neutral component neutral component purified purified T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HRV3C protease cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Asn501Tyr;Arg682_Arg685delinsGlySerAlaSer;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain RRAR GSAS certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain KV PP Fri Sep 18 23:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.Lys986_Val987delinsProPro [986-987]' Mon Jun 28 17:08:46 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Asn501Tyr;Lys986_Val987delinsProPro;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Arg682_Arg683delinsGlySer;Arg685Ser]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Table 1 s-81 2 5 unspecified role unspecified role neutral component neutral component purified purified T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HRV3C protease cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Asn501Tyr;Arg682_Arg685delinsGlySerAlaSer;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain RRAR GSAS certain certain sequence position certain certain certain sequence position certain N Y certain certain sequence position certain certain certain sequence position certain KV PP Fri Sep 18 23:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.Lys986_Val987delinsProPro [986-987]' Mon Jun 28 16:53:33 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Asn501Tyr;Lys986_Val987delinsProPro;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Arg682_Arg683delinsGlySer;Arg685Ser]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Table 1 ace2-s-93 3 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role bait bait purified purified T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HRV3C protease cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Arg682_Arg685delinsGlySerAlaSer;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain RRAR GSAS certain certain sequence position certain certain certain sequence position certain KV PP Fri Sep 18 23:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.Lys986_Val987delinsProPro [986-987]' Mon Jun 28 17:02:36 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Asn501Tyr;Lys986_Val987delinsProPro;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Arg682_Arg683delinsGlySer;Arg685Ser]' P0DTC2:p.Asn501Tyr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N Y Mon Jun 28 17:22:15 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Figure 2 C/D ace2-s-95 1 4 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey purified purified T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range HRV3C protease cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.[Arg682_Arg685delinsGlySerAlaSer;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain F P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain A P certain certain sequence position certain certain certain sequence position certain RRAR GSAS certain certain sequence position certain certain certain sequence position certain KV PP Fri Sep 18 23:50:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.Lys986_Val987delinsProPro [986-987]' Mon Jun 28 17:03:05 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC2:p.[Asn501Tyr;Lys986_Val987delinsProPro;Phe817Pro;Ala892Pro;Ala899Pro;Ala942Pro;Arg682_Arg683delinsGlySer;Arg685Ser]' P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Supp. Figure 5 C/D, Table S1.