IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. Science advances 2021 imex curation Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Accepted 2021-OCT-06 AT 10:08 BST AT 10:08 BST by ORCHARD Science advances 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. Science advances 2021 imex curation Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Accepted 2021-OCT-06 AT 10:08 BST AT 10:08 BST by ORCHARD Science advances 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. Science advances 2021 imex curation Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Only protein-protein interactions imex curation in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Changes in intrinsic fluorescence were monitored over a range of temperatures. peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Accepted 2021-OCT-06 AT 10:08 BST AT 10:08 BST by ORCHARD Science advances 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. Science advances 2021 imex curation Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Accepted 2021-OCT-06 AT 10:08 BST AT 10:08 BST by ORCHARD Science advances 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. SARS-CoV-2 can recruit a heme metabolite to evade antibody immunity. Science advances 2021 imex curation Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined pH 5.0 8.0 6.0 7.0 5.5 Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P., Rosa A., Pye VE., Graham C., Muir L., Seow J., Ng KW., Cook NJ., Rees-Spear C., Parker E., Dos Santos MS., Rosadas C., Susana A., Rhys H., Nans A., Masino L., Roustan C., Christodoulou E., Ulferts R., Wrobel AG., Short CE., Fertleman M., Sanders RW., Heaney J., Spyer M., Kjær S., Riddell A., Malim MH., Beale R., MacRae JI., Taylor GP., Nastouli E., van Gils MJ., Rosenthal PB., Pizzato M., McClure MO., Tedder RS., Kassiotis G., McCoy LE., Doores KJ., Cherepanov P. Virus - Publications including interactions involving viral proteins peter.cherepanov@crick.ac.uk, katie.doores@ kcl.ac.uk, l.mccoy@ucl.ac.uk, george.kassiotis@crick.ac.uk Accepted 2021-OCT-06 AT 10:08 BST AT 10:08 BST by ORCHARD Science advances 2021 Only protein-protein interactions imex curation imex curation spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT bilirubin ixalpha bilirubin IXalpha 1,10,19,22,23,24-hexahydro-2,7,13,17-tetramethyl-1,19-dioxo-3,18-divinylbiline-8,12-dipropionic acid 2,17-diethenyl-1,10,19,22,23,24-hexahydro-3,7,13,18-tetramethyl-1,19-dioxo-21H-biline-8,12-dipropanoic acid 2,7,13,17-tetramethyl-1,19-dioxo-3,18-divinyl-1,10,19,22,23,24-hexahydro-21H-biline-8,12-dipropanoic acid 8,12-bis(2-carboxyethyl)-2,7,13,17-tetramethyl-3,18-divinylbiladiene-ac-1,19(21H,24H)-dione Bilirubin bilirubin(Z,Z) 3,18-diethenyl-2,7,13,17-tetramethyl-1,19-dioxo-1,10,19,22,23,24-hexahydro-21H-biline-8,12-dipropanoic acid bilirubin bilirubin-IXalpha small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) biliverdin biliverdin 8,12-bis(2-carboxyethyl)-2,7,13,17-tetramethyl-3,18-divinylbilin-1(19)(21H,24H)-dione Biliverdin Biliverdin IX alpha Biliverdin IXalpha Biliverdine BILIVERDINE IX ALPHA biliverdin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hemin-1 hemin chloro(protoporphyrinato)iron(III) chloro[3,7,12,17-tetramethyl-8,13-divinylporphyrin-2,18-dipropanoato(2-)]iron(III) chlorohemin chloroprotoferrihem ferriprotoporphyrin IX chloride Haemin hemin Hemin hemin chloride hemin IX Hemine hemine Panhematin protohemin protohemin IX chlorido(protoporphyrinato)iron(III) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) biliverdin-s-1 1 12 unspecified role unspecified role neutral component neutral component purified purified foldon trimerisation tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV-cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Lys986_Val987delinsProPro mutation mutation certain certain sequence position certain certain certain sequence position certain KV PP mu-phosphatase secretion leader sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Table S2 biliverdin-s-2 4 12 unspecified role unspecified role neutral component neutral component purified purified N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Table S3 s-biliverdin-1 5 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 7 Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-biliverdin-2 5 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 10 Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-biliverdin-3 5 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role prey prey direct interaction direct interaction 6 Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-biliverdin-4 5 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role prey prey direct interaction direct interaction 8 Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-hemin_1-1 2 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-biliverdin-6 2 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. biliverdin-s-3 3 12 unspecified role unspecified role neutral component neutral component purified purified N-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Figure S3K The melting point of isolated NTD increased by over 8 degrees C in the presence of biliverdin. s-biliverdin-7 2 14 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Arg190Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R K Tue Oct 05 19:29:56 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg190Lys' P0DTC2:p.Asn121Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N Q Tue Oct 05 18:45:41 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn121Gln' human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-biliverdin-8 5 12 unspecified role unspecified role bait bait purified purified C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 14 unspecified role unspecified role prey prey direct interaction direct interaction 9 Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct. s-bilirubin ixalpha-1 2 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait purified purified P0DTC2:p.Asn121Gln mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N Q C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Supp. figure 3. Table S1 as far as I can make out, the strep tag has only been removed from NTD Spike construct.