IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins A "Fluorescence-3-hybrid" Assay. A GFP binder protein (GBP) is attached to a protein that accumulates at a well-defined location within the cell. This complex recruits to that particular location GFP-tagged proteins. If the GFP tagged protein interacts with a second protein, labelled with a different fluorescent marker, the interaction can be immediately visualized using fluorescent microscopy. rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. The EMBO journal 2021 imex curation Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Lei J., Ma-Lauer Y., Han Y., Thoms M., Buschauer R., Jores J., Thiel V., Beckmann R., Deng W., Leonhardt H., Hilgenfeld R., von Brunn A. Virus - Publications including interactions involving viral proteins rolf.hilgenfeld@uni-luebeck.de,vonbrunn@mvp.lmu.de Accepted 2021-JUL-19 AT 07:17 BST AT 07:17 BST by MARTA The EMBO journal 2021 Only protein-protein interactions imex curation imex curation paip1_human Polyadenylate-binding protein-interacting protein 1 PAIP1 protein protein human Homo sapiens Human MSDGFDRAPGAGRGRSRGLGRGGGGPEGGGFPNGAGPAERARHQPPQPKAPGFLQPPPLRQPRTTPPPGAQCEVPASPQRPSRPGALPEQTRPLRAPPSSQDKIPQQNSESAMAKPQVVVAPVLMSKLSVNAPEFYPSGYSSSYTESYEDGCEDYPTLSEYVQDFLNHLTEQPGSFETEIEQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ A4820607190A3A43 p0dtc1-pro_0000449637 Papain-like protease nsp3 ORF1a polyprotein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 FFFD2D956385E317 q9h074-2 Polyadenylate-binding protein-interacting protein 1 PAIP1 protein protein human Homo sapiens Human MSDGFDRAPEQTRPLRAPPSSQDKIPQQNSESAMAKPQVVVAPVLMSKLSVNAPEFYPSGYSSSYTESYEDGCEDYPTLSEYVQDFLNHLTEQPGSFETEIEQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ 9743587A6F54BA25 pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV DIP protein P11940 original sequence version: 187 DIP protein P11940 original sequence version: 181 DIP protein P11940-1 original sequence version: 136 DIP protein P11940 original sequence version: 148 DIP protein P11940 original sequence version: 172 2EB1B02A346132EE p0c6u8-pro_0000338257 Papain-like protease nsp3 ORF1a polyprotein 1a protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus APIKGVTFGEDTVWEVQGYKNVRITFELDERVDKVLNEKCSVYTVESGTEVTEFACVVAEAVVKTLQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENFSSRMYCSFYPPDEEEEDDAECEEEEIDETCEHEYGTEDDYQGLPLEFGASAETVRVEEEEEEDWLDDTTEQSEIEPEPEPTPEEPVNQFTGYLKLTDNVAIKCVDIVKEAQSANPMVIVNAANIHLKHGGGVAGALNKATNGAMQKESDDYIKLNGPLTVGGSCLLSGHNLAKKCLHVVGPNLNAGEDIQLLKAAYENFNSQDILLAPLLSAGIFGAKPLQSLQVCVQTVRTQVYIAVNDKALYEQVVMDYLDNLKPRVEAPKQEEPPNTEDSKTEEKSVVQKPVDVKPKIKACIDEVTTTLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEKDAPYMVGDVITSGDITCVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPGQGCAGYTLEEAKTALKKCKSAFYVLPSEAPNAKEEILGTVSWNLREMLAHAEETRKLMPICMDVRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPVASIITKLNSLNEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPDAVTTYNGYLTSSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDKIVYHTLESPVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCGRDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDGVTYTEIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNTKFADDLNQMTGFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPIVWHINQATTKTTFKPNTWCLRCLWSTKPVDTSNSFEVLAVEDTQGMDNLACESQQPTSEEVVENPTIQKEVIECDVKTTEVVGNVILKPSDEGVKVTQELGHEDLMAAYVENTSITIKKPNELSLALGLKTIATHGIAAINSVPWSKILAYVKPFLGQAAITTSNCAKRLAQRVFNNYMPYVFTLLFQLCTFTKSTNSRIRASLPTTIAKNSVKSVAKLCLDAGINYVKSPKFSKLFTIAMWLLLLSICLGSLICVTAAFGVLLSNFGAPSYCNGVRELYLNSSNVTTMDFCEGSFPCSICLSGLDSLDSYPALETIQVTISSYKLDLTILGLAAEWVLAYMLFTKFFYLLGLSAIMQVFFGYFASHFISNSWLMWFIISIVQMAPVSAMVRMYIFFASFYYIWKSYVHIMDGCTSSTCMMCYKRNRATRVECTTIVNGMKRSFYVYANGGRGFCKTHNWNCLNCDTFCTGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVAVKNGALHLYFDKAGQKTYERHPLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCDESASKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKMFDAYVDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQGVVDTDVDTKDVIECLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGACIDCNARHINAQVAKSHNVSLIWNVKDYMSLSEQLRKQIRSAAKKNNIPFRLTCATTRQVVNVITTKISLKGG 819 2740 BBB1A6190358ADDE p0c6u8_pro_0000338257-paip1-1 6 11 unspecified role unspecified role neutral component neutral component purified purified M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 16 18 direct interaction direct interaction Supplementary Table 1 p0c6u8_pro_0000338257-paip1-2 8 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N_terminal_delta_16_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 21 24 physical association physical association 1B/D/F, 3 E/F p0c6u8_pro_0000338257-paip1-3 3 11 unspecified role unspecified role neutral component neutral component purified purified M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain N_terminal_delta_16_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 31 29 direct interaction direct interaction 1G, 3A, Supp. figure 2,3B p0c6u8_pro_0000338257-paip1-4 1 11 unspecified role unspecified role neutral component neutral component purified purified M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain N_terminal_delta_16_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Mac1–2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 35 40 36 38 direct interaction direct interaction 3 B/C/D pabpc1-paip1-8 8 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B Co-expression of excess SUD-RFP did not interfere with binding between PABP and Paip1. p0c6u8_pro_0000338257-paip1-5 8 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed SUD_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A Co-expression of excess RFP-PABP did not result in an impaired interaction between Paip1 and SUD. pabpc1-p0c6u8_pro_0000338257-1 4 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed SUD_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed M region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4C p0c6u8_pro_0000338257-paip1-6 8 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region nyfp nYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cyfp cYFP undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined N_terminal_delta_16_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 65 68 physical association physical association 6 B/C p0c6u8_pro_0000338257-paip1-7 2 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NLS tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined delta_16_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain NLS tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supp. figure 1 A/B, 3A/C All transfected constructs had an NLS (Nuclear localisation signal) attached. pabpc1-paip1-9 5 9 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction EV1A p0c6u8_pro_0000338257-pabpc1-1 5 13 stimulator stimulator neutral component neutral component purified purified SUD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association EV1B SUD enhances the binding affinity of PABP to Paip1 by about X 4.4. p0c6u8_pro_0000338257-paip1-8 7 11 unspecified role unspecified role neutral component neutral component purified purified M region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified MAC2_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 95 97 direct interaction direct interaction Supplementary Table 2