IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. I cannot find any PArticipant Detection; so entered "wt by staining" as the most likely one. Crosslinker = glutaraldehyde. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight by staining molecular weight estimation by staining weight by staining Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. Turbidity measurements at 340nm. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro light microscopy light microscopy predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. we do not know the stoichiometry for the 4S Free RNA See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Multivalent binding of the partially disordered SARS-CoV-2 nucleocapsid phosphoprotein dimer to RNA. Biophysical journal 2021 imex curation Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) ion mobility mass spec ion mobility mass spectrometry of complexes ion mobility mass spec predetermined predetermined participant predetermined Forsythe HM., Galvan JR., Yu Z., Pinckney S., Reardon P., Cooley RB., Zhu P., Rolland AD., Prell JS., Barbar E. See pmid:33290746 for details of 1000 residue RNA. barbare@oregonstate.edu Accepted 2021-APR-29 AT 15:19 BST AT 15:19 BST by ORCHARD Biophysical journal 2021 Only protein-protein interactions imex curation imex curation genomic_rna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA n-88 10 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 2A, 4B Both FL-N and N-CTD are predominantly dimers in solution. n-89 5 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction Fig. 2C, Supp. figure S1 FL-N shows a large broad peak, indicative of a relatively more disordered structure sampling multiple conformations. Dimerization constant of 0.4 uM for FL-N where the dimer dissociation constant (Kd) is defined as the ratio of the concentrations of the monomers and dimers in the steady state. n-90 5 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Fig. 2B, Supp. figure S2 A single sharp peak was seen. Dimerization constant of 1 uM for CTD where the dimer dissociation constant (Kd) is defined as the ratio of the concentrations of the monomers and dimers at the steady state. n-91 12 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range direct interaction direct interaction 2D IM-MS clearly indicates the presence of both a monomer and dimer native ion population. genomic_rna-n-22 7 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) colocalization colocalization 4A FL-N forms droplet-like aggregates in the presence of 1–1000 RNA. genomic_rna-n-23 3 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4B n-94 1 14 unspecified role unspecified role neutral component neutral component purified purified NTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction There is a small dimer population (~30 kDa) only observed at the higher protein concentration of 60 uM. 4C n-92 1 13 unspecified role unspecified role neutral component neutral component 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified NTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4C In the presence of RNA higher-order NTD populations appeared (>40 kDa). In the presence of Urea the higher aggregates are not formed. 123234wer-1 1 14 unspecified role unspecified role neutral component neutral component purified purified CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4D Cross-linking of CTD without RNA shows a band corresponding to the CTD dimer. n-93 1 13 unspecified role unspecified role neutral component neutral component 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4D genomic_rna-n-24 1 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4E In the absence of RNA very faint bands can be seen showing formation of an array of heterogeneous larger oligomers. With the addition of RNA, it is stated that FL-N forms a complex too large to migrate through the gel. With the addition of Urea and RNA to FL-N, it is stated that the higher aggregates are not formed, however only very faint bands are present. I see a lot of nothing for figure 4E, however image and labels are poor for figs 4C-E. genomic_rna-n-25 1 14 unspecified role unspecified role neutral component neutral component purified purified TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role neutral component neutral component purified purified TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 4E In the absence of RNA very faint bands can be seen showing formation of an array of heterogeneous larger oligomers. I see a lot of nothing for figure 4E, however image and labels are poor for figs 4C-E. genomic_rna-n-26 2 13 unspecified role unspecified role bait bait purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role prey prey purified purified CTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5A, Lanes 4-6, 5B. A smear of larger complexes were seen (figure 5A) with FL-N. they give a Kd value- but from the text it is unclear which construct it applies to - so not added. genomic_rna-n-27 2 13 unspecified role unspecified role unspecified role unspecified role purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role unspecified role unspecified role purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction 5A, Lane 2 In the absence of protein several bands are seen of >1000 nucleic acid residues. genomic_rna-n-28 2 13 unspecified role unspecified role bait bait 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role prey prey purified purified NTD sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 5B genomic_rna-n-29 9 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) colocalization colocalization 5C genomic_rna-n-30 11 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5D genomic_rna-n-33 6 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified regions required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 6 B/C Turbidity is highest with FL-N and when RNA is bound at the lower pH.of 6.5. However there is only a significant difference between free N and RNA bound at a concentration of 50 uM FL-N. genomic_rna-n-31 8 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5 E/F Free RNA sediments as a single broad peak with a sedimentation coefficient of 4 S. In contrast, FL-N/1–1000 RNA produced a heterogeneous sedimentation profile with s-values on a scale X10 greater than free RNA, suggesting formation of multiple large complexes of variable stoichiometry. genomic_rna-n-32 4 13 unspecified role unspecified role neutral component neutral component purified purified 5' utr sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 14 unspecified role unspecified role neutral component neutral component purified purified C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range TEV cleavage site tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 7 The majority of the peaks within the N-terminal domain disappear on binding.