IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Inhibition of HECT E3 ligases as potential therapy for COVID-19. Inhibition of HECT E3 ligases as potential therapy for COVID-19. Cell death & disease 2021 imex curation Novelli G., Liu J., Biancolella M., Alonzi T., Novelli A., Patten JJ., Cocciadiferro D., Agolini E., Colona VL., Rizzacasa B., Giannini R., Bigio B., Goletti D., Capobianchi MR., Grelli S., Mann J., McKee TD., Cheng K., Amanat F., Krammer F., Guarracino A., Pepe G., Tomino C., Tandjaoui-Lambiotte Y., Uzunhan Y., Tubiana S., Ghosn J., null null., null null., null null., Notarangelo LD., Su HC., Abel L., Cobat A., Elhanan G., Grzymski JJ., Latini A., Sidhu SS., Jain S., Davey RA., Casanova JL., Wei W., Pandolfi PP. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FLAG-tagged constructs of NEDD4, WWP1/2, have been deduced to be of human origin based on mutation mapping for constructs given in Figure 5 for NEDD4/WWP1 mutants. NEDD4 maps to P46934-3 and WWP1 to Q9H0M0-1. Smurf1 maps to either the Murine or Human protein thus interactions involving this protein have not been curated owing to the ambiguity of it's origins. The authors did not reply to a request for information. novelli@med.uniroma2.it,pierpaolo.pandolfiderinaldis@renown.org Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down anti tag western anti tag western blot anti tag western Novelli G., Liu J., Biancolella M., Alonzi T., Novelli A., Patten JJ., Cocciadiferro D., Agolini E., Colona VL., Rizzacasa B., Giannini R., Bigio B., Goletti D., Capobianchi MR., Grelli S., Mann J., McKee TD., Cheng K., Amanat F., Krammer F., Guarracino A., Pepe G., Tomino C., Tandjaoui-Lambiotte Y., Uzunhan Y., Tubiana S., Ghosn J., null null., null null., null null., Notarangelo LD., Su HC., Abel L., Cobat A., Elhanan G., Grzymski JJ., Latini A., Sidhu SS., Jain S., Davey RA., Casanova JL., Wei W., Pandolfi PP. Virus - Publications including interactions involving viral proteins FLAG-tagged constructs of NEDD4, WWP1/2, have been deduced to be of human origin based on mutation mapping for constructs given in Figure 5 for NEDD4/WWP1 mutants. NEDD4 maps to P46934-3 and WWP1 to Q9H0M0-1. Smurf1 maps to either the Murine or Human protein thus interactions involving this protein have not been curated owing to the ambiguity of it's origins. The authors did not reply to a request for information. novelli@med.uniroma2.it,pierpaolo.pandolfiderinaldis@renown.org Accepted 2021-JUN-29 AT 11:53 BST AT 11:53 BST by KALPANAP Cell death & disease 2021 Only protein-protein interactions imex curation imex curation Inhibition of HECT E3 ligases as potential therapy for COVID-19. Inhibition of HECT E3 ligases as potential therapy for COVID-19. Cell death & disease 2021 imex curation Novelli G., Liu J., Biancolella M., Alonzi T., Novelli A., Patten JJ., Cocciadiferro D., Agolini E., Colona VL., Rizzacasa B., Giannini R., Bigio B., Goletti D., Capobianchi MR., Grelli S., Mann J., McKee TD., Cheng K., Amanat F., Krammer F., Guarracino A., Pepe G., Tomino C., Tandjaoui-Lambiotte Y., Uzunhan Y., Tubiana S., Ghosn J., null null., null null., null null., Notarangelo LD., Su HC., Abel L., Cobat A., Elhanan G., Grzymski JJ., Latini A., Sidhu SS., Jain S., Davey RA., Casanova JL., Wei W., Pandolfi PP. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FLAG-tagged constructs of NEDD4, WWP1/2, have been deduced to be of human origin based on mutation mapping for constructs given in Figure 5 for NEDD4/WWP1 mutants. NEDD4 maps to P46934-3 and WWP1 to Q9H0M0-1. Smurf1 maps to either the Murine or Human protein thus interactions involving this protein have not been curated owing to the ambiguity of it's origins. The authors did not reply to a request for information. novelli@med.uniroma2.it,pierpaolo.pandolfiderinaldis@renown.org Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Novelli G., Liu J., Biancolella M., Alonzi T., Novelli A., Patten JJ., Cocciadiferro D., Agolini E., Colona VL., Rizzacasa B., Giannini R., Bigio B., Goletti D., Capobianchi MR., Grelli S., Mann J., McKee TD., Cheng K., Amanat F., Krammer F., Guarracino A., Pepe G., Tomino C., Tandjaoui-Lambiotte Y., Uzunhan Y., Tubiana S., Ghosn J., null null., null null., null null., Notarangelo LD., Su HC., Abel L., Cobat A., Elhanan G., Grzymski JJ., Latini A., Sidhu SS., Jain S., Davey RA., Casanova JL., Wei W., Pandolfi PP. Virus - Publications including interactions involving viral proteins FLAG-tagged constructs of NEDD4, WWP1/2, have been deduced to be of human origin based on mutation mapping for constructs given in Figure 5 for NEDD4/WWP1 mutants. NEDD4 maps to P46934-3 and WWP1 to Q9H0M0-1. Smurf1 maps to either the Murine or Human protein thus interactions involving this protein have not been curated owing to the ambiguity of it's origins. The authors did not reply to a request for information. novelli@med.uniroma2.it,pierpaolo.pandolfiderinaldis@renown.org Accepted 2021-JUN-29 AT 11:53 BST AT 11:53 BST by KALPANAP Cell death & disease 2021 Only protein-protein interactions imex curation imex curation wwp2_human NEDD4-like E3 ubiquitin-protein ligase WWP2 WW domain-containing protein 2 Atrophin-1-interacting protein 2 WWP2 HECT-type E3 ubiquitin transferase WWP2 protein protein human Homo sapiens Human MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIILNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENMQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGNVPNGSALTDGSQLPSRDSSGTAVAPENRHQPPSTNCFGGRSRTHRHSGASARTTPATGEQSPGARSRHRQPVKNSGHSGLANGTVNDEPTTATDPEEPSVVGVTSPPAAPLSVTPNPNTTSLPAPATPAEGEEPSTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELYFIQDMEILGKVTTHELKEGGESIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKSTIYRHYTKNSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDKVGKETWLPRSHTCFNRLDLPPYKSYEQLREKLLYAIEETEGFGQE FCCD75CBA61F2204 q9h0m0-1 NEDD4-like E3 ubiquitin-protein ligase WWP1 WWP1 Atrophin-1-interacting protein 5 HECT-type E3 ubiquitin transferase WWP1 TGIF-interacting ubiquitin ligase 1 WW domain-containing protein 1 A protein protein human Homo sapiens Human MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTETLPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRRRVYYVDHNTRTTTWQRPTMESVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWEDPRTQGLQNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERGFRWKLAHFRYLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKDLESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRFSRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFVKETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 35B6E1C03A3147DA ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG p46934-3 E3 ubiquitin-protein ligase NEDD4 HECT-type E3 ubiquitin transferase NEDD4 NEDD4 Cell proliferation-inducing gene 53 protein KIAA0093 NEDD4-1 Neural precursor cell expressed developmentally down-regulated protein 4 PIG53 protein protein human Homo sapiens Human MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQERSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHLVSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDSTSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTSPTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSLQKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTSACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFASGNEGKVDNLSRDSNRDCTNELSNSCKPGWVVLDQPDAACHLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD No experimental confirmation available. 827AC55FB4AFB6A3 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT nedd4-s-1 2 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P0DTC2:p.Pro25_Tyr28delinsAlaAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PPAY AAAA Thu Apr 22 13:06:34 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro25_Tyr28delinsAlaAlaAlaAla' P0DTC2:p.Pro25_Tyr28del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PPAY . physical association physical association 1A the 4 amino acid deletion cannot be entered as "required to Bind" as it is not. wwp1-s-1 2 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9H0M0-1:p.Lys740Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K N Thu Apr 22 10:18:46 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys740Asn' Q9H0M0-1:p.Asn745Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N S Thu Apr 22 13:06:36 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn745Ser' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1A, 5 ubiq_human-nedd4-1 1 6 stimulator stimulator prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1D Ectopic expression of NEDD4 could promote the ubiquitination of the SARS-CoV-2 S protein. this could to be regarded as a PTM (which we do not curate) - but as reaction with ubiquitin can also be considered as an "interaction" I took that view here, as info may be lost otherwise. nedd4-s-2 2 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P46934-3:p.Ile843Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain I R Thu Apr 22 13:29:56 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile843Arg' P46934-3:p.Arg877Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain R Q Thu Apr 22 12:50:52 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg877Gln' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5A wwp2-s-1 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1A