IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3. Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3. Journal of gastrointestinal oncology 2021 imex curation Du Q., Ye X., Lu SR., Li H., Liu HY., Zhai Q., Yu B. yubo_phd@yeah.net Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Du Q., Ye X., Lu SR., Li H., Liu HY., Zhai Q., Yu B. yubo_phd@yeah.net Accepted 2021-AUG-02 AT 18:16 BST AT 18:16 BST by KALPANAP Journal of gastrointestinal oncology 2021 Only protein-protein interactions imex curation imex curation hsa-mir-30a-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UGUAAACAUCCUCGACUGGAAG mrna_mia3 MIA SH3 domain ER export factor 3 mRna mrna messenger rna mRNA mrna human Homo sapiens Human GAGGTGACCACAACATGGCTGCGGCGCCTGGGCTGCTCGTCTGGCTGCTCGTGCTCCGGCTGCCCTGGCGGGTGCCGGGCCAGCTGGACCCCAGCACTGGCCGGCGGTTCTCGGAGCACAAACTCTGCGCGGACGACGAATGCAGCATGTTAATGTACCGCGGTGAGGCTCTTGAAGATTTCACAGGCCCGGATTGTCGTTTTGTGAATTTTAAAAAAGGTGATCCTGTATATGTTTACTATAAACTGGCAAGAGGATGGCCTGAAGTTTGGGCTGGAAGTGTTGGACGCACTTTTGGATATTTTCCAAAAGATTTAATCCAGGTAGTTCATGAATATACCAAAGAAGAGCTACAAGTTCCAACAGATGAGACGGATTTTGTTTGTTTTGATGGAGGAAGAGATGATTTTCATAATTATAATGTAGAAGAACTTTTAGGGTTTTTGGAACTGTACAATTCTGCAGCTACAGATTCTGAGAAAGCTGTAGAAAAAACTTTACAGGATATGGAAAAAAACCCTGAATTATCTAAGGAAAGGGAACCTGAACCTGAACCAGTAGAAGCCAACTCAGAGGAAAGTGATAGTGTATTCTCAGAAAACACTGAGGATCTTCAGGAACAGTTTACAACTCAGAAGCACCACTCCCATGCAAACAGCCAAGCAAATCATGCTCAGGGAGAGCAGGCTTCATTTGAATCTTTTGAAGAAATGCTGCAAGATAAACTAAAAGTGCCAGAAAGTGAAAACAACAAAACCAGCAATAGTTCTCAGGTCTCAAATGAACAGGATAAGATTGATGCCTATAAACTTTTGAAAAAAGAAATGACTCTAGACTTGAAAACCAAATTTGGCTCAACAGCTGATGCACTTGTATCTGATGATGAGACAACCAGACTCGTTACTTCATTAGAAGATGATTTTGATGAGGAATTGGATACTGAGTATTATGCAGTTGGAAAGGAAGATGAGGAGAACCAAGAAGACTTTGATGAGTTGCCATTACTTACCTTTACAGATGGGGAAGATATGAAAACTCCAGCAAAGTCTGGCGTTGAGAAATATCCAACAGATAAAGAGCAGAATTCAAATGAAGAGGACAAGGTTCAGCTAACTGTGCCCCCTGGCATCAAAAATGATGATAAAAATATACTAACAACCTGGGGGGACACTATCTTCTCTATTGTCACAGGAGGTGAAGAAACAAGAGATACGATGGATTTAGAGAGCTCTAGTTCAGAGGAAGAAAAAGAAGATGATGATGATGCATTAGTCCCAGATAGCAAACAGGGGAAACCACAGTCAGCAACAGATTATAGTGACCCTGACAATGTAGATGATGGTCTTTTTATTGTAGACATTCCTAAAACAAATAATGACAAAGAAGTAAACGCAGAACATCACATTAAAGGAAAAGGGAGGGGAGTTCAGGAATCCAAGAGGGGCCTGGTACAAGATAAGACAGAATTAGAGGATGAAAATCAAGAAGGCATGACTGTGCACAGTTCTGTTCACAGCAATAACCTCAACTCTATGCCAGCTGCTGAAAAGGGTAAAGACACATTAAAATCAGCTTATGATGATACAGAAAATGACCTAAAAGGAGCAGCTATTCATATCTCAAAAGGAATGCTCCACGAAGAAAAGCCTGGAGAGCAGATTTTGGAAGGTGGCTCAGAGAGTGAATCTGCACAGAAAGCTGCAGGGAATCAAATGAATGACAGAAAGATTCAACAGGAATCCCTGGGTAGTGCACCACTCATGGGAGATGACCACCCTAACGCATCCAGAGACAGTGTGGAGGGAGACGCTTTGGTAAATGGGGCCAAACTGCACACGCTTTCAGTGGAGCATCAACGTGAGGAATTGAAAGAGGAATTAGTTCTTAAAACTCAAAACCAACCTAGATTCTCCTCTCCAGATGAGATTGATTTGCCCAGAGAACTGGAAGACGAGGTTCCCATTCTGGGAAGAAATCTTCCCTGGCAACAAGAAAGAGATGTGGCTGCCACAGCCAGTAAGCAAATGAGTGAGAAGATAAGGCTCTCTGAGGGAGAAGCCAAAGAGGACTCCTTGGATGAAGAGTTTTTTCATCACAAGGCAATGCAGGGCACAGAGGTAGGACAGACAGACCAAACTGACAGCACAGGAGGACCAGCTTTCCTTTCTAAAGTAGAAGAGGATGATTATCCCTCTGAAGAACTACTAGAGGATGAAAACGCTATAAATGCAAAACGGTCTAAAGAAAAAAACCCTGGGAATCAGGGCAGGCAGTTTGATGTTAATCTGCAAGTCCCTGACAGAGCAGTTTTAGGGACCATTCATCCAGATCCAGAAATTGAAGAAAGCAAGCAAGAAACTAGTATGATTTTGGATAGCGAAAAAACAAGTGAGACTGCTGCCAAAGGGGTCAACACAGGAGGCAGGGAACCAAATACAATGGTGGAAAAAGAACGCCCTCTGGCAGATAAGAAAGCACAGAGACCATTTGAACGAAGTGACTTTTCTGACAGCATAAAAATTCAGACTCCAGAATTAGGTGAAGTGTTTCAGAATAAAGATTCTGATTATCTGAAGAACGACAACCCTGAGGAACATCTGAAGACCTCAGGGCTTGCAGGGGAGCCTGAGGGAGAACTCTCAAAAGAGGACCATGAGAACACAGAGAAGTACATGGGCACAGAAAGCCAGGGGTCTGCTGCTGCAGAACCTGAAGATGACTCGTTCCACTGGACTCCACATACAAGTGTAGAGCCAGGGCATAGTGACAAGAGGGAGGACTTACTTATCATAAGCAGCTTCTTTAAAGAACAACAGTCTTTGCAGCGGTTCCAGAAGTACTTTAATGTCCATGAGCTGGAAGCCTTGCTACAAGAAATGTCATCAAAACTGAAGTCAGCGCAGCAGGAGAGCCTGCCCTATAATATGGAAAAAGTCCTAGATAAGGTCTTCCGTGCTTCTGAGTCACAAATTCTGAGCATAGCAGAAAAAATGCTTGATACTCGTGTGGCTGAAAATAGAGATCTGGGAATGAACGAAAATAACATATTTGAAGAGGCTGCAGTGCTTGATGACATTCAAGACCTCATCTATTTTGTCAGGTACAAGCACTCCACAGCAGAGGAGACAGCCACACTGGTGATGGCACCACCTCTAGAGGAAGGCTTGGGTGGAGCAATGGAAGAGATGCAACCACTGCATGAAGATAATTTCTCACGAGAGAAGACAGCAGAACTTAATGTGCAGGTTCCTGAAGAACCCACCCACTTGGACCAACGTGTGATTGGGGACACTCATGCCTCAGAAGTGTCACAGAAGCCAAATACTGAGAAAGACCTGGACCCAGGGCCAGTTACAACAGAAGACACTCCTATGGATGCTATTGATGCAAACAAGCAACCAGAGACAGCCGCCGAAGAGCCGGCAAGTGTCACACCTTTGGAAAACGCAATCCTTCTAATATATTCATTCATGTTTTATTTAACTAAGTCGCTAGTTGCTACATTGCCTGATGATGTTCAGCCTGGGCCTGATTTTTATGGACTGCCATGGAAACCTGTATTTATCACTGCCTTCTTGGGAATTGCTTCGTTTGCCATTTTCTTATGGAGAACTGTCCTTGTTGTGAAGGATAGAGTATATCAAGTCACGGAACAGCAAATTTCTGAGAAGTTGAAGACTATCATGAAAGAAAATACAGAACTTGTACAAAAATTGTCAAATTATGAACAGAAGATCAAGGAATCAAAGAAACATGTTCAGGAAACCAGGAAACAAAATATGATTCTCTCTGATGAAGCAATTAAATATAAGGATAAAATCAAGACACTTGAAAAAAATCAGGAAATTCTGGATGACACAGCTAAAAATCTTCGTGTTATGCTAGAATCTGAGAGAGAACAGAATGTCAAGAATCAGGACTTGATATCAGAAAACAAGAAATCTATAGAGAAGTTAAAGGATGTTATTTCAATGAATGCCTCAGAATTTTCAGAGGTTCAGATTGCACTTAATGAAGCTAAGCTTAGTGAAGAGAAGGTGAAGTCTGAATGCCATCGGGTTCAAGAAGAAAATGCTAGGCTTAAGAAGAAAAAAGAGCAGTTGCAGCAGGAAATCGAAGACTGGAGTAAATTACATGCTGAGCTCAGTGAGCAAATCAAATCATTTGAGAAGTCTCAGAAAGATTTGGAAGTAGCTCTTACTCACAAGGATGATAATATTAATGCTTTGACTAACTGCATTACACAGTTGAATCTGTTAGAGTGTGAATCTGAATCTGAGGGTCAAAATAAAGGTGGAAATGATTCAGATGAATTAGCAAATGGAGAAGTGGGAGGTGACCGGAATGAGAAGATGAAAAATCAAATTAAGCAGATGATGGATGTCTCTCGGACACAGACTGCAATATCGGTAGTTGAAGAGGATCTAAAGCTTTTACAGCTTAAGCTAAGAGCCTCCGTGTCCACTAAATGTAACCTGGAAGACCAGGTAAAGAAATTGGAAGATGACCGCAACTCACTACAAGCTGCCAAAGCTGGACTGGAAGATGAATGCAAAACCTTGAGGCAGAAAGTGGAGATTCTGAATGAGCTCTATCAGCAGAAGGAGATGGCTTTGCAAAAGAAACTGAGTCAAGAAGAGTATGAACGGCAAGAAAGAGAGCACAGGCTGTCAGCTGCAGATGAAAAGGCAGTTTCGGCTGCAGAGGAAGTAAAAACTTACAAGCGGAGAATTGAAGAAATGGAGGATGAATTACAGAAGACAGAGCGGTCATTTAAAAACCAGATCGCTACCCATGAGAAGAAAGCTCATGAAAACTGGCTCAAAGCTCGTGCTGCAGAAAGAGCTATAGCTGAAGAGAAAAGGGAAGCTGCCAATTTGAGACACAAATTATTAGAATTAACACAAAAGATGGCAATGCTGCAAGAAGAACCTGTGATTGTAAAACCAATGCCAGGAAAACCAAATACACAAAACCCTCCACGGAGAGGTCCTCTGAGCCAGAATGGCTCTTTTGGCCCATCCCCTGTGAGTGGTGGAGAATGCTCCCCTCCATTGACAGTGGAGCCACCCGTGAGACCTCTCTCTGCTACTCTCAATCGAAGAGATATGCCTAGAAGTGAATTTGGATCAGTGGACGGGCCTCTACCTCATCCTCGATGGTCAGCTGAGGCATCTGGGAAACCCTCTCCTTCTGATCCAGGATCTGGTACAGCTACCATGATGAACAGCAGCTCAAGAGGCTCTTCCCCTACCAGGGTACTCGATGAAGGCAAGGTTAATATGGCTCCAAAAGGGCCCCCTCCTTTCCCAGGAGTCCCTCTCATGAGCACCCCCATGGGAGGCCCTGTACCACCACCCATTCGATATGGACCACCACCTCAGCTCTGCGGACCTTTTGGGCCTCGGCCACTTCCTCCACCCTTTGGCCCTGGTATGCGTCCACCACTAGGCTTAAGAGAATTTGCACCAGGCGTTCCACCAGGAAGACGGGACCTGCCTCTCCACCCTCGGGGATTTTTACCTGGACACGCACCATTTAGACCTTTAGGTTCACTTGGCCCAAGAGAGTACTTTATTCCTGGTACCCGATTACCACCCCCAACCCATGGTCCCCAGGAATACCCACCACCACCTGCTGTAAGAGACTTACTGCCGTCAGGCTCTAGAGATGAGCCTCCACCTGCCTCTCAGAGCACTAGCCAGGACTGTTCACAGGCTTTAAAACAGAGCCCATAAAACTATGACCTCTGAGGTTTCATTGGAAAGAAAGTGTACTGTGCATTATCCATTACAGTAAAGGATTTCATTGGCTTCAAAATCCAAAAGTTTATTTTAAAAGGTTTGTTGTTAGAACTAAGCTGCCTTGGCAGTGTGCATTTTTGAGCCAAACAATTCAAAAATGTCATTTCTTCCCTAAATAAAAATCACCTTTTAAGCTAGAGCGTCCTTACAACTTTGAAATGTGCAATAAAGAATACCTGTGTTTTAGCTAATGTAGCATATGTAATTGCAAAATGATTTAGAATGTCATGAAAAATATGAACATTTCCTGTGGAAATGCTTTAAGAACATGTATTTCCATTATCCTATTTTTAGTGTACACCAGCTGAATACGGAGCAATGGTGTTTATAAGCGTTTTTTTAAACTATCTGGTCACAAAGACTGTTACGCTAAAAATGTTTACTAAAAGATCACTAAACTATCTCCCCTCTTGCTGAAGTTCTTTGTAGTAATAGCTCATAAAAATTTGTTTATTAATATTTCCCAAGTGTCTGTTGACTCATTGGACTGTTATGAGGCTTGTGCCATTTGGGGAACATGTAAACTCAGGCTCCCAGAACTGAAGATGGTGGCTGGTGGCACACTTCCGGCTGCTCCTCCGTCACCTGTGAACTCTACAAGTGACGTCTTTTTATTTCAAAGAAGTTTATTTCCCACTTGTATAGCATTCACATGCTTTCTTTACGATCCTCATTGTCTATTTGAGAATGGTTTTCTGAGAGTGAGTTTACATTAGTAGCAAGAGTTGTTTGACCTGATGTTCCATTGTTTTTACCATTCCTGTAGAAAAAGGGTGCACAACAGAAAAATGAAAATGATGTGTCATGGCCATAAAAGTATAGAAATCTTTAAAAATTTTAAAATGTACAGTCCCTTATCTATCTTTCCCATTCCTTGCCACTGATTTTTGAGGAATATAATAAAAAGATTGGAAGAGTATAATGCCATGAGAAAGAATGATTTAGGACTGTGAGGGTTATAACATGCCCTAGGTCAGCAACCAAGGGTTGAAATCAGTTCTGTTTTAGGGGGAAATGGGGGCGACAGATATTATTCCAAAATTAATATTAATTAATATTTAAACGTTGGTGTTTTTATTTAAAAATCAGTAACTAACCATCTGGAATTGCACCATACTTAAAGTCTTATCCATTACTACACTGTCTTTAAAACAATGTTTCTTTAAATACTCTACAACGTTTCTAAGAACGAACTTCAGACATTTTAATTACAGTAATAATAGCACTCCTTTTAAGGAGTTTCAGATCCACACTAAAACTAAAATCATAAAAGGCTGATACTTTTGTTTGCTGCTAGGCTATATTCTTCCATTCTTTGAAGTCCTATGATGTAATATTTTTGAAACCTAGTGTATGTCTTGTCACTGTTGTGATATTTAATCGATTAAGAATACCTTGTAAAAAGGAGCAAAAGCTTCAATGTGAAACAATTTTCTCTCTTTATACTAAACAACTGAAGATAGATAGTTTAGAAAGATAAGGACCTTTGAAAGAAGACAACTCTGTCAAAGTTCATAAGGAATATAAAAATTCTTCAGGAAAAGAGAATTCAATCTATATGTCCTCCCGTTTAATATCAAGAATAGAAGAAATTAAGAGGAAAACTCCACAGAAGAGCATAGGCCACTTTTAGCCATGTAAAAATAAGATTAAGTCACAAATACAACTTTTGAATTTACCTGTCAATATCTCTTTAGGACACAAAACAATGCTGAAGTTAATATAATTTCTAATTTTAAATGTCATTTAAGTGTAGATTATGCCATCTAGGAAGGTAAGTAGGAAAGGTAAATTAAATCTATTTTTAAAATTCAAAATATTAGAGTATTTTTCCCCTCTAAAGCCTTTTTTGGTGATTATTCTGTATCTGACATAATTGAGAAACTGGTAAGCTGTAAAGATTCCAGTGTAGCTTCTCTGAGAAGTTGTGAGCCAGTCCATAACTGCTTCCTCACATCCATCTGATTGCACCATTTCTGCAGCAAACCCCAAAGCAGGGTGCCAATATGCAGATGGCATAGGGAGTATCATCCCTCAGCCAAATCACTTTTCCATCTCTAAAGTTTCATCTATTTTGGAAGTCATCTCCAACTAATTGTGTCTGGATTTAGTTGCTAAAATTGTCTTATTTATGAAGCAGCAATATTCAGCCTGAAAGCATTTCTGCCATAGTTGTTGTAGTTATATCGCCAATGGCTGATTTTTTTCATTGGAAAGTAAATTTAAGTAATTCGTGGGATGTGGTATATTCTGTGTCAACTTCAAGATAATCACTCATTTTCTCGTTATATTCAGGTCTGAATTAAAGTTAAGTTAATCACA hsa-mir-222-3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human AGCUACAUCUGGCUACUGGGU hsa_mir_222_3p-mrna_mia3-1 1 4 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000344922:r.5996_6001delinsacatcg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TGTAGC ACATCG physical association physical association Fig 6 C D E down-regulates quantity by destabilization hsa_mir_30a_5p-mrna_mia3-1 1 2 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range ENST00000344922:r.6181_6186delinsacaaat mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain TGTTTA ACAAAT physical association physical association Fig 6 C D E down-regulates quantity by destabilization