IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Cell 2021 imex curation Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk Accepted 2021-APR-01 AT 19:59 BST AT 19:59 BST by KALPANAP Cell 2021 Only protein-protein interactions imex curation imex curation Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Cell 2021 imex curation Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins a shame that GISAID data is not publically available. Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk Accepted 2021-APR-01 AT 19:59 BST AT 19:59 BST by KALPANAP Cell 2021 Only protein-protein interactions imex curation imex curation Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity. Cell 2021 imex curation Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Thomson EC., Rosen LE., Shepherd JG., Spreafico R., da Silva Filipe A., Wojcechowskyj JA., Davis C., Piccoli L., Pascall DJ., Dillen J., Lytras S., Czudnochowski N., Shah R., Meury M., Jesudason N., De Marco A., Li K., Bassi J., O'Toole A., Pinto D., Colquhoun RM., Culap K., Jackson B., Zatta F., Rambaut A., Jaconi S., Sreenu VB., Nix J., Zhang I., Jarrett RF., Glass WG., Beltramello M., Nomikou K., Pizzuto M., Tong L., Cameroni E., Croll TI., Johnson N., Di Iulio J., Wickenhagen A., Ceschi A., Harbison AM., Mair D., Ferrari P., Smollett K., Sallusto F., Carmichael S., Garzoni C., Nichols J., Galli M., Hughes J., Riva A., Ho A., Schiuma M., Semple MG., Openshaw PJM., Fadda E., Baillie JK., Chodera JD., null null., null null., Rihn SJ., Lycett SJ., Virgin HW., Telenti A., Corti D., Robertson DL., Snell G. Virus - Publications including interactions involving viral proteins Sequences for RBD domains other than SARS2 are not explicitly stated;these have been taken from the Uniprot entries corresponding to the Accession number given in Table S1. gsnell@vir.bio, david.l.robertson@glasgow.ac.uk Accepted 2021-APR-01 AT 19:59 BST AT 19:59 BST by KALPANAP Cell 2021 Only protein-protein interactions imex curation imex curation a0a7d6p0r3_9betc Spike glycoprotein S E2 Peplomer protein protein protein Pangolin coronavirus Pangolin coronavirus MLFFFFLHFALVNSQCVNLTGRAAIQPSFTNSSQRGVYYPDTIFRSNTLVLSQGYFLPFYSNVSWYYALTKTNSAEKRVDNPVLDFKDGIYFAATEKSNIVRGWIFGTTLDNTSQSLLIVNNATNVIIKVCNFQFCYDPYLSGYYHNNKTWSTREFAVYSSYANCTFEYVSKSFMLDIAGKSGLFDTLREFVFRNVDGYFKIYSKYTPVNVNSNLPIGFSALEPLVEIPAGINITKFRTLLTIHRGDPMPNNGWTVFSAAYYVGYLAPRTFMLNYNENGTITDAVDCALDPLSEAKCTLKSLTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATTFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVRGDEVRQIAPGQTGRIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFHPTNGVGYQPYRVVVLSFELLNAPATVCGPKQSTNLVKNKCVNFNFNGLTGTGVLTESSKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWSVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSRSVSSQAIIAYTMSLGAENSVAYANNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSIECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPSQEKNFTTTPAICHEGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGSCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIIMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 55EAAF652C201911 spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 a0a6g6a2r8_9betc Spike glycoprotein S E2 Peplomer protein protein protein Pangolin coronavirus Pangolin coronavirus MFVFLFVLPLVSSQCVNLTTRTGIQPGYTNSSTRGVYYPDKVFRSSILHLTQDLFLPFFSNVTWFNTINYQGGFKKFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDARTQSLLIVNNATNVVIKVCEFQFCTDPFLGVYYHNNNKTWVENEFRVYSSANNCTFEYISQPFLMDLEGKQGNFKNLREFVFKNVDGYFKIYSKHTPIDLVRDLPRGFAALEPLVDLPIGINITRFQTLLALHRSYLTPGKLESGWTTGAAAYYVGYLQQRTFLLSYNQNGTITDAVDCSLDPLSETKCTLKSLTVEKGIYQTSNFRVQPTISIVRFPNITNLCPFGEVFNASKFASVYAWNRKRISNCVADYSVLYNSTSFSTFKCYGVSPTKLNDLCFTNVYADSFVVKGDEVRQIAPGQTGVIADYNYKLPDDFTGCVIAWNSVKQDALTGGNYLYRLFRKSKLKPFERDISTEIYQAGSTPCNGQVGLNCYYPLERYGFHPTTGVNYQPFRVVVLSFELLNGPATVCGPKLSTTLVKDKCVNFNFNGLTGTGVLTTSKKQFLPFQQFGRDISDTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPMAIHAEQLTPAWRVYSAGANVFQTRAGCLVGAEHVNNSYECDIPVGAGICASYHSMSSLRSVNQRSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSIECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHEGKAHFPREGVFVSNGTHWFITQRNFYEPQIITTDNTFVSGSCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIIMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 82E0542840BA1CA3 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT u5wi05_sars Spike glycoprotein S E2 Peplomer protein protein protein Bat SARS-like coronavirus WIV1 Bat SARS-like coronavirus WIV1 MKLLVLVFATLVSSYTIEKCLDFDDRTPPANTQFLSSHRGVYYPDDIFRSNVLHLVQDHFLPFDSNVTRFITFGLNFDNPIIPFKDGIYFAATEKSNVIRGWVFGSTMNNKSQSVIIMNNSTNLVIRACNFELCDNPFFVVLKSNNTQIPSYIFNNAFNCTFEYVSKDFNLDLGEKPGNFKDLREFVFRNKDGFLHVYSGYQPISAASGLPTGFNALKPIFKLPLGINITNFRTLLTAFPPRPDYWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVAPSKEVVRFPNITNLCPFGEVFNATTFPSVYAWERKRISNCVADYSVLYNSTSFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFTGCVLAWNTRNIDATQTGNYNYKYRSLRHGKLRPFERDISNVPFSPDGKPCTPPAFNCYWPLNDYGFYITNGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNTSSEVAVLYQDVNCTDVPVAIHADQLTPSWRVHSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSSLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAVEQDRNTREVFAQVKQMYKTPTLKDFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGSCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT 6BDAE351DE821C93 s-ace2-65 2 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction S2C I do not know if the RBD Spike construct has the C-terminal thrombin cleavage site, or not, as unclear which construct they used-most likely the second as the other viruses are mentioned for that one. SARS2 virus s-ace2-66 2 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 9 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction S2C WIV1 Virus s-ace2-67 2 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 4 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction S2C GD Pangolin Virus s-ace2-68 2 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 7 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction S2C GX Pangolin Virus s-ace2-69 2 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 5 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind range range range range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction S2C SARS Virus s-ace2-70 3 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 8 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.Asn439Lys mutation mutation certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:38:17 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction Supp. Table 3 The SARS-CoV-2 RBD N439K-hACE2 complex was formed together with two Fab fragments (S304 and S309) to aid in crystallization. (the sequences of the Fab fragments have not been included.) if i recall correctly, we do not antibody sequences as the Update will insist on trying to update them even if we add the "no-uniprot-update" annotation to them. s-ace2-71 1 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 8 unspecified role unspecified role bait bait purified purified avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mu-phosphatase signal peptide tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC2:p.[Asp614Gly;Gln677_Arg685delinsArg;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain D G certain certain sequence position certain certain certain sequence position certain QTNSPRRAR R certain certain sequence position certain certain certain sequence position certain KV PP P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:36:06 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.Lys417Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K V Mon Mar 01 11:45:30 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys417Val' P0DTC2:p.[Lys417Val;Asn439Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K V certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:08:32 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn439Lys;Lys417Val]' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 4D, number 1 I cannot see the purple line for the double mutant; it must be underneath the WT one. s-ace2-72 1 6 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain avi-tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 8 unspecified role unspecified role bait bait purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Lys417Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K V Mon Mar 01 11:07:39 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys417Val' P0DTC2:p.[Lys417Val;Asn439Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K V certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:09:54 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn439Lys;Lys417Val]' P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:11:12 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.[Lys417Val;Asn439Arg] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K V certain certain sequence position certain certain certain sequence position certain N R Mon Mar 01 11:24:40 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn439Arg;Lys417Val]' P0DTC2:p.Asn439Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N R Mon Mar 01 10:50:50 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Arg' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 4D, number 2 s-ace2-73 1 6 unspecified role unspecified role bait bait purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus fc-igg2 fc-igg2 c-term range C-terminal range c-term range c-term range C-terminal range c-term range unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 8 unspecified role unspecified role prey prey purified purified unknown signal sequence tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range thrombin cleavage site tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Lys417Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K V Mon Mar 01 11:36:21 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys417Val' P0DTC2:p.[Lys417Val;Asn439Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K V certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:13:00 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn439Lys;Lys417Val]' P0DTC2:p.Asn439Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N K Mon Mar 01 11:12:13 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Lys' P0DTC2:p.[Lys417Val;Asn439Arg] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K V certain certain sequence position certain certain certain sequence position certain N R Mon Mar 01 11:39:42 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn439Arg;Lys417Val]' P0DTC2:p.Asn439Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N R Mon Mar 01 10:49:29 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn439Arg' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 4D, number 3 I assume the Spike protein is the same as previous one?