IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. ACS central science 2021 imex curation Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu Accepted 2021-MAR-01 AT 13:04 GMT AT 13:04 GMT by PPORRAS libraries of up to ~10-E8 random synthetic peptides. ACS central science 2021 Only protein-protein interactions imex curation imex curation <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. ACS central science 2021 imex curation Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu Accepted 2021-MAR-01 AT 13:04 GMT AT 13:04 GMT by PPORRAS libraries of up to ~10-E8 random synthetic peptides. ACS central science 2021 Only protein-protein interactions imex curation imex curation <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. ACS central science 2021 imex curation Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu Accepted 2021-MAR-01 AT 13:04 GMT AT 13:04 GMT by PPORRAS ACS central science 2021 Only protein-protein interactions imex curation imex curation <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. <i>De Novo</i> Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein. ACS central science 2021 imex curation Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL., Pomplun S., Jbara M., Quartararo AJ., Zhang G., Brown JS., Lee YC., Ye X., Hanna S., Pentelute BL. blp@mit.edu Accepted 2021-MAR-01 AT 13:04 GMT AT 13:04 GMT by PPORRAS ACS central science 2021 Only protein-protein interactions imex curation imex curation peptide_1 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) TVFGLNVWKRYSK peptide_4 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LVMGLNVWLRYSK ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 spike_mers1 Spike glycoprotein E2 Peplomer protein 3 S protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC MIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRFLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFHKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH D73276432FA5D515 peptide_2 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LVMGLNAWNMWYK peptide_3 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) LVMGLHVYLRQGK spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT s-peptide_1-1 2 12 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 10 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) association association 1A s-peptide_1-3 1 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_1:p.Lys13delinsLysXaaLys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 10:50:52 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' peptide_1:p.Thr1Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Mon Mar 01 11:48:31 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1Ala' peptide_1:p.Val2Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain V A Mon Mar 01 11:48:42 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val2Ala' peptide_1:p.Phe3Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Mon Mar 01 11:03:30 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe3Ala' peptide_1:p.Gly4Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G A Mon Mar 01 11:41:00 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly4Ala' peptide_1:p.Leu5Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Mon Mar 01 11:40:48 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu5Ala' peptide_1:p.Asn6Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Mon Mar 01 11:11:19 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn6Ala' peptide_1:p.Val7Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Mon Mar 01 11:12:49 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val7Ala' peptide_1:p.Trp8Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W A Mon Mar 01 10:57:28 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp8Ala' peptide_1:p.Lys9Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Mon Mar 01 11:07:50 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys9Ala' peptide_1:p.Arg10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Mon Mar 01 11:19:18 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg10Ala' peptide_1:p.Tyr11Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Mon Mar 01 10:54:45 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr11Ala' peptide_1:p.Ser12Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Mon Mar 01 11:50:05 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser12Ala' peptide_1:p.Lys13Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Mon Mar 01 10:52:33 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13Ala' peptide_1:p.Thr1del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T . Mon Mar 01 11:43:52 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1del' peptide_1:p.Thr1_Val2del mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain TV .. Mon Mar 01 11:13:29 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1_Val2del' peptide_1:p.Ser12_Lys13del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain SK .. Mon Mar 01 11:07:36 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser12_Lys13del' peptide_1:p.Tyr11_Lys13del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain YSK ... Mon Mar 01 11:34:10 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr11_Lys13del' Thr1 acthr monoacetylated L-threonine AcThr certain certain sequence position certain certain certain sequence position certain decreasing-ptm ptm decreasing an interaction decreasing-ptm chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B/C, 2, Supp. figure 8, Table S1 peptide_1-s-1 1 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 10 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_2:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:01:56 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B peptide_2-s-1 1 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 11 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_3:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:03:10 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B peptide_4-s-1 1 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_4:p.Lys13delinsLysXaaLys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 10:53:29 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1B peptide_1-s-2 1 5 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:49:26 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 1D peptide_1-s-3 1 8 unspecified role unspecified role prey prey purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:32:31 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3A No details are stated for SARS1 and MERS proteins regarding amino acid ranges or expression system(s) or tag(s). RBD range was taken from Uniprot. peptide_1-s-4 1 5 unspecified role unspecified role bait bait peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:43:42 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role prey prey purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction 3A No details are stated for SARS1 and MERS proteins regarding amino acid ranges or expression system(s) or tag(s). RBD range was taken from Uniprot. RBD range was taken from Uniprot. peptide_1-s-5 1 12 unspecified role unspecified role prey prey purified purified S1 region sufficient to bind sufficient binding region sufficient to bind range range range range 9mamm mammals 5 unspecified role unspecified role bait bait peptide_1:p.Lys13delinsLysXaaLys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 10:51:17 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. figure 7 No details are stated for S1 or trimer proteins regarding amino acid ranges or exact expression system(s) or tag(s). peptide_1-s-6 1 5 unspecified role unspecified role bait bait peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:35:35 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role prey prey purified purified direct interaction direct interaction Supp. figure 7 No details are stated for S1 or trimer proteins regarding amino acid ranges or exact expression system(s) or tag(s). peptide_1-s-7 3 12 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role bait bait C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:16:13 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4, Supp. figure 9. Spike-RBD was added to human serum and then pulled down by Peptide-1;no other proteins were detected. peptide_1-s-8 4 12 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 unspecified role unspecified role prey prey C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range peptide_1:p.Lys13delinsLysXaaLys p.Lys13delinsB-alalys mutation mutation certain certain sequence position certain certain certain sequence position certain K KXK Mon Mar 01 11:02:26 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys13delinsB-alalys' chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 5, Supp. figure 10, 11 Similar results were obtained in experiments with SARS-CoV-2-spike-RBD dissolved in human serum. ace2-s-84 1 7 unspecified role unspecified role bait bait purified purified C-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 12 unspecified role unspecified role prey prey purified purified rbd sufficient to bind sufficient binding region sufficient to bind range range range range 9inse 9inse direct interaction direct interaction Supp. figure 6 It is not stated which insect derived Spike RBD construct was used- as given in Supplementary ref. 4 (Zhang-2020 -no pmid). Peptide-1 does not compete with ACE2 binding to the RBD.