IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-16hbe 14o Homo sapiens 16hbe 14o 16hbe 14o confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. LLPS (liquid-liquid phase separation) observed with a Nikon spinning disk microscope. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. Turbidity measurements were conducted at 600 nm. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) light microscopy light microscopy predetermined predetermined participant predetermined Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. LLPS (liquid-liquid phase separation) observed with a Nikon spinning disk microscope with a X60 oil immersion lens. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. SARS-CoV-2 nucleocapsid protein phase separates with G3BPs to disassemble stress granules and facilitate viral production. Science bulletin 2021 imex curation Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Luo L., Li Z., Zhao T., Ju X., Ma P., Jin B., Zhou Y., He S., Huang J., Xu X., Zou Y., Li P., Liang A., Liu J., Chi T., Huang X., Ding Q., Jin Z., Huang C., Zhang Y. Virus - Publications including interactions involving viral proteins FRAP experiments:figure 2H not annotated as apart from just possibly showing N-N interactions are more stable than G3BP1-G3BP1 ones, I do not see what to curate, certainly not an "N- G3BP1" interaction. Figure 3C:again - only mobility data after interaction so comment added to Expt-5 only. huangxx@shanghaitech.edu.cn, qding@tsinghua.edu.cn, 27zgkin@zjnu.edu.cn, chuang@shutcm.edu.cn, zhangy@shanghaitech.edu.cn Accepted 2021-MAR-25 AT 13:20 GMT AT 13:20 GMT by KALPANAP Science bulletin 2021 Only protein-protein interactions imex curation imex curation ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA capr1_human Caprin-1 CAPRIN1 GPIAP1 GPIP137 M11S1 Cytoplasmic activation- and proliferation-associated protein 1 GPI-anchored membrane protein 1 GPI-anchored protein p137 Cell cycle-associated protein 1 Membrane component chromosome 11 surface marker 1 RNG105 RNA granule protein 105 protein protein human Homo sapiens Human MPSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGTGAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNNLEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAEEYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN 56F5BC188CA3A2D4 fmr1_human Synaptic functional regulator FMR1 FMR1 Fragile X mental retardation protein 1 protein protein human Homo sapiens Human MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGKVIGKNGKLIQEIVDKSGVVRVRIEAENEKNVPQEEEIMPPNSLPSNNSRVGPNAPEEKKHLDIKENSTHFSQPNSTKVQRVLVASSVVAGESQKPELKAWQGMVPFVFVGTKDSIANATVLLDYHLNYLKEVDQLRLERLQIDEQLRQIGASSRPPPNRTDKEKSYVTDDGQGMGRGSRPYRNRGHGRRGPGYTSGTNSEASNASETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTDNRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPDSVDGQQPLVNGVP DIP protein Q06787 original sequence version: 68 F853D6C82E3489B9 g3bp1_human Ras GTPase-activating protein-binding protein 1 G3BP1 G3BP ATP-dependent DNA helicase VIII GAP SH3 domain-binding protein 1 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ 0F9429D78E0C7F59 e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC DIP protein P19525 original sequence version: 164 DIP protein P19525-1 original sequence version: 177 DIP protein P19525-1 original sequence version: 178 DIP protein P19525-1 original sequence version: 170 DIP protein P19525 original sequence version: 153 815AD83ACAB45DA3 g3bp2_human Ras GTPase-activating protein-binding protein 2 GAP SH3 domain-binding protein 2 G3BP2 KIAA0660 protein protein human Homo sapiens Human MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNFSECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSSSGIPPHVKAPVSQPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR DIP protein Q9UN86 original sequence version: 147 74B8EA43C0560229 ubp10_human Ubiquitin carboxyl-terminal hydrolase 10 USP10 KIAA0190 Ubiquitin-specific-processing protease 10 Deubiquitinating enzyme 10 Ubiquitin thioesterase 10 protein protein human Homo sapiens Human MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL E6BA77E2B5CE2B3F g3bp1-n-1 1 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined IDR1 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization 1A, 3 E/F, Supp. figure 1 A/E, 5 Cells were treated with sodium arsenite, poly(I:C) or DTT to induce Stress Granules. Stress Granule assembly was markedly inhibited in the majority of N protein-transfected cells. I assume Supp figure 5 is also in this cell line, as none other is given. The N protein mutant lacking IDR1 (51-C) was unable to colocalize with G3BP1 or to disassemble Stress Granuless in vivo. g3bp1-n-2 9 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 1C Stress Granule induction by sodium arsenite. g3bp1-n-3 4 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization 1D, Supp. figure 1B Cells were treated with sodium arsenite, poly(I:C) or DTT to induce Stress Granules. Stress Granule assembly was markedly inhibited in the majority of N protein-transfected cells. cycloheximide (CHEBI:27641); disrupted N protein/G3BP foci. n-n-50 8 10 unspecified role unspecified role neutral component neutral component purified purified 10 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction 2 A/B, Supp. figure 3 A/B Ficoll - a crowding agent Total HeLa RNA At physiological salt concentration, N protein formed droplets when the protein concentration reached 15 umol/L. n-n-51 5 10 unspecified role unspecified role neutral component neutral component purified purified region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 C Total HeLa RNA RNase A This was carried out in the presence of HeLa RNA. g3bp1-n-4 5 13 unspecified role unspecified role neutral component neutral component purified purified region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain IDR1 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 D-F, 3 B/C, Supp. figure 4 A-C Total HeLa RNA RNase A Results from figure 3C (FRAP) show that while IDR1 triggers the N protein LLPS, the C-terminus stabilizes the condensate. n-n-52 7 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 2G Total HeLa RNA n-n-53 7 13 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 2G Total HeLa RNA n-n-54 7 13 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 2G Total HeLa RNA n-g3bp1-1 3 15 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain IDR1 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 3 G/H RNase g3bp2-usp10-7 2 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4D SARS2 Flag-N protein (P0DTC9) n-g3bp1-3 2 15 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed NTF2L domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A g3bp1-eif2ak2-1 6 16 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 13 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 14 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association 4B SARS2 Flag-N protein (P0DTC9) eif2ak2-g3bp2-1 2 15 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4E SARS2 Flag-N protein (P0DTC9) n-caprin1-1 9 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Supp. figure 1D weak interaction.