IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain. ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain. Biochemical and biophysical research communications 2021 imex curation Dang M., Li Y., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dbssjx@nus.edu.sg 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro light microscopy light microscopy predetermined predetermined participant predetermined Dang M., Li Y., Song J. Virus - Publications including interactions involving viral proteins Differential interference contrast microscopy. Turbidity, the absorption at 600 nm, was also measured. dbssjx@nus.edu.sg Accepted 2021-FEB-04 AT 03:53 GMT AT 03:53 GMT by KALPANAP Biochemical and biophysical research communications 2021 Only protein-protein interactions imex curation imex curation ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain. ATP biphasically modulates LLPS of SARS-CoV-2 nucleocapsid protein and specifically binds its RNA-binding domain. Biochemical and biophysical research communications 2021 imex curation Dang M., Li Y., Song J. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus dbssjx@nus.edu.sg 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Dang M., Li Y., Song J. Virus - Publications including interactions involving viral proteins dbssjx@nus.edu.sg Accepted 2021-FEB-04 AT 03:53 GMT AT 03:53 GMT by KALPANAP Biochemical and biophysical research communications 2021 Only protein-protein interactions imex curation imex curation ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atp-n-1 1 4 stimulator stimulator neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 C-E Upon addition of ATP, the turbidity values of the N protein samples increased. However above a 1:200 (N-protein:ATP) further increase of ATP concentrations led to a decrease in turbidity. Droplet formation proceeded as for turbidity, with an increase in ATP concentration leading to a reduction of droplet numbers. A24:The ratios required to induce and dissolve droplets were much lower than those for ATP. Similarly to ATP, addition of more ATP to A24 samples led to a reduction of turbidity values, as well as disappearance of droplets. atp-n-3 2 4 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 The RBD has no significant binding with ATP at [ATP] <1mM.