DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution. Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution. FEBS letters 2020 imex curation Golubev A., Fatkhullin B., Khusainov I., Jenner L., Gabdulkhakov A., Validov S., Yusupova G., Yusupov M., Usachev K. k.usachev@kpfu.ru Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Golubev A., Fatkhullin B., Khusainov I., Jenner L., Gabdulkhakov A., Validov S., Yusupova G., Yusupov M., Usachev K. k.usachev@kpfu.ru Accepted 2021-AUG-03 AT 12:25 BST AT 12:25 BST by KALPANAP FEBS letters 2020 Only protein-protein interactions imex curation imex curation rs5_staa8 30S ribosomal protein S5 rpsE SAOUHSC_02494 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNGRVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPGTGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKTVEELYN 9D8BDE4D1E7D7056 rl6_staa8 50S ribosomal protein L6 rplF SAOUHSC_02496 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFKQEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKDLILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGKGIRYQGEYVRRKEGKTGK 62A50C86B3689C40 rl29_staa8 50S ribosomal protein L29 rpmC SAOUHSC_02504 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIRTVRKTIARLKTVAREREIEQSKANQ DE23A549C6A54496 q2g0s0_staa8 50S ribosomal protein L25 General stress protein CTC rplY ctc SAOUHSC_00474 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVDEVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTVEVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVKVTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE 1F6A316EA0A45108 rl17_staa8 50S ribosomal protein L17 rplQ SAOUHSC_02484 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVVEKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTRILKQGPRRGDGAESVIIELV BB10FD48584A867E rs20_staa8 30S ribosomal protein S20 rpsT SAOUHSC_01689 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNADNKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK 2C5AA2FAC1F47BC2 rl18_staa8 50S ribosomal protein L18 rplR SAOUHSC_02495 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQIIDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYHGRVKALAEAARESGLEF 515ECF8471D6B6EB rl21_staa8 50S ribosomal protein L21 rplU SAOUHSC_01757 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGDSVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA 0D3AA2949F0F45FD rl4_staa8 50S ribosomal protein L4 rplD SAOUHSC_02511 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASLRQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMPKKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENEDVNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG 43A13417F457C6CC rl16_staa8 50S ribosomal protein L16 rplP SAOUHSC_02505 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITSRQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRILFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES 27B45FD1C2540213 rs17_staa8 30S ribosomal protein S17 rpsQ SAOUHSC_02503 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSKKYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII 576EF20791F19DD6 rs6_staa8 30S ribosomal protein S6 rpsF SAOUHSC_00348 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWGKRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK B188B60F2938B4F1 rl28_staa8 50S ribosomal protein L28 rpmB SAOUHSC_01191 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKPKKVWVSARALKSGKVTRV 20042609E7D9C26A rs8_staa8 30S ribosomal protein S8 rpsH SAOUHSC_02498 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEGFIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLGIALVSTSEGVITDKEARKRNVGGEIIAYVW 3625209D6101757E rl36_staa8 50S ribosomal protein L36 rpmJ SAOUHSC_02488 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG 7F79BEC2C6BF4CF7 rl20_staa8 50S ribosomal protein L20 rplT SAOUHSC_01784 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQYAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISDEKAFAQLVTKAKDALK 108789019A553B89 rl19_staa8 50S ribosomal protein L19 rplS SAOUHSC_01211 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQVFEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYLRSLRGKAARIQEIR BA30AF1820FC2E70 rl15_staa8 50S ribosomal protein L15 rplO SAOUHSC_02492 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGGGVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVVKNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI 6CB42B627B38F93E rs12_staa8 30S ribosomal protein S12 rpsL SAOUHSC_00527 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKRGVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN 5E973910C6F97995 rl2_staa8 50S ribosomal protein L2 rplB SAOUHSC_02509 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKRYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGASAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKKK F29296D3EE1E146E rl34_staa8 50S ribosomal protein L34 rpmH SAOUHSC_03055 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLSA 488B186AB2504E16 rl13_staa8 50S ribosomal protein L13 rplM SAOUHSC_02478 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVTYTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLIENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG 9757E9AF942BC042 rl32_staa8 50S ribosomal protein L32 rpmF SAOUHSC_01078 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGREYKLSHRVCKNCGSYNGEEVAAK 5A5C930A5EB8DEC4 rs19_staa8 30S ribosomal protein S19 rpsS SAOUHSC_02508 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNFIGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR BEB53BBDB77DAA33 rl24_staa8 50S ribosomal protein L24 rplX SAOUHSC_02501 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKHQKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIKSNN 30DE6E9B3BF74B50 rs14_staa8 30S ribosomal protein S14 rpsN rpsN2 SAOUHSC_01329 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSSPTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW C1AB8AABC6940349 rl3_staa8 50S ribosomal protein L3 rplC SAOUHSC_02512 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVDGYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVGQEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDASRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKGNK 27BAE1308C8F0675 rs11_staa8 30S ribosomal protein S11 rpsK SAOUHSC_02486 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSWSSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQSAGLEVTAIRDVTPVPHNGCRPPKRRRV 4596857561FB031D rs4_staa8 30S ribosomal protein S4 rpsD SAOUHSC_01829 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKLSEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLGLARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNFVPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR E4BBB131EF37A6ED rl31b_staa8 50S ribosomal protein L31 type B rpmE2 SAOUHSC_02361 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYPVIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN 28CA86AE4FBDEE01 rl27_staa8 50S ribosomal protein L27 rpmA SAOUHSC_01755 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSILYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE 9EDE9C2774CAAEAA rs7_staa8 30S ribosomal protein S7 rpsG SAOUHSC_00528 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILYSAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLRWLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW 8FE24C11A747D0AB rs3_staa8 30S ribosomal protein S3 rpsC SAOUHSC_02506 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDNELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINVIEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGADIARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK 174CA582EB0DF917 rs2_staa8 30S ribosomal protein S2 rpsB SAOUHSC_01232 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQKTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLTNYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQALFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMADAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE 2049566B86927839 rl14_staa8 50S ribosomal protein L14 rplN SAOUHSC_02502 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVKNATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPVARELREGNFMKIVSLAPEVL CB890728A314786E rl35_staa8 50S ribosomal protein L35 rpmI SAOUHSC_01785 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQLRKARLVSKSDMKRVKQLLAYKK 3AD91136263DC335 rs16_staa8 30S ribosomal protein S16 rpsP SAOUHSC_01208 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTSANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK 62305117338000C5 rs15_staa8 30S ribosomal protein S15 rpsO SAOUHSC_01250 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLRTHKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR 356A7EEB3A43E431 rl30_staa8 50S ribosomal protein L30 rpmD SAOUHSC_02493 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIRGQINKVKHLVTVEEK 6625A0E67A7C26B4 rs18_staa8 30S ribosomal protein S18 rpsR SAOUHSC_00350 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKILPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ B02C5C4F05C4D185 rs13_staa8 30S ribosomal protein S13 rpsM SAOUHSC_02487 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTRVKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKTKNNARTRKGPVKTVANKKK 4223639C52DA5FD8 rl5_staa8 50S ribosomal protein L5 rplE SAOUHSC_02500 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAVQNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDKLISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTANTDEEARELLANFGMPFRK 3A0070FDF84DF93B rs9_staa8 30S ribosomal protein S9 rpsI SAOUHSC_02477 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFESLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKRAGLLTRDPRMKERKKPGLKAARRSPQFSKR D9178675589E4B0A rl332_staa8 50S ribosomal protein L33 2 rpmG2 SAOUHSC_01651 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTLHRETK 07E4F257A9B22DD3 rl23_staa8 50S ribosomal protein L23 rplW SAOUHSC_02510 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEEIFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN A8F978ED9DE8092C a0a2x3yy77_staa8 30S ribosomal protein S10 NCTC8325_02294 rpsJ protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPTEKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL 6FBFAA4DE6EB8805 rl22_staa8 50S ribosomal protein L22 rplV SAOUHSC_02507 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASSPVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSHITIVVSDGKEEAKEA 98D1E37BEE51DE4B rplb-rplc-30 1 16 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 37 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 22 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 45 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 24 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 31 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 40 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 14 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 32 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 26 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 46 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 48 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 17 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 18 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 11 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 19 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 36 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 23 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 43 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 28 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 21 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 25 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 41 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 12 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 38 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 39 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 27 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 42 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 20 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 29 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 47 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 44 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 15 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 33 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 13 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 2 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 30 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 34 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified 35 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig 1, Fig S1, Table S2