DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation. Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation. Communications biology 2020 imex curation Nakamura R., Numata T., Kasuya G., Yokoyama T., Nishizawa T., Kusakizako T., Kato T., Hagino T., Dohmae N., Inoue M., Watanabe K., Ichijo H., Kikkawa M., Shirouzu M., Jentsch TJ., Ishitani R., Okada Y., Nureki O. nureki@bs.s.u-tokyo.ac.jp Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Nakamura R., Numata T., Kasuya G., Yokoyama T., Nishizawa T., Kusakizako T., Kato T., Hagino T., Dohmae N., Inoue M., Watanabe K., Ichijo H., Kikkawa M., Shirouzu M., Jentsch TJ., Ishitani R., Okada Y., Nureki O. nureki@bs.s.u-tokyo.ac.jp Accepted 2021-OCT-07 AT 14:20 BST AT 14:20 BST by MGT Communications biology 2020 Only protein-protein interactions imex curation imex curation Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation. Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation. Communications biology 2020 imex curation Nakamura R., Numata T., Kasuya G., Yokoyama T., Nishizawa T., Kusakizako T., Kato T., Hagino T., Dohmae N., Inoue M., Watanabe K., Ichijo H., Kikkawa M., Shirouzu M., Jentsch TJ., Ishitani R., Okada Y., Nureki O. nureki@bs.s.u-tokyo.ac.jp Only protein-protein interactions imex curation spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Nakamura R., Numata T., Kasuya G., Yokoyama T., Nishizawa T., Kusakizako T., Kato T., Hagino T., Dohmae N., Inoue M., Watanabe K., Ichijo H., Kikkawa M., Shirouzu M., Jentsch TJ., Ishitani R., Okada Y., Nureki O. nureki@bs.s.u-tokyo.ac.jp Accepted 2021-OCT-07 AT 14:20 BST AT 14:20 BST by MGT Communications biology 2020 Only protein-protein interactions imex curation imex curation lrc8d_human Volume-regulated anion channel subunit LRRC8D Leucine-rich repeat-containing protein 8D UNQ213/PRO239 LRRC8D LRRC5 Leucine-rich repeat-containing protein 5 protein protein human Homo sapiens Human MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNAEVTTNIPKMEAATNQDQDGRTTNDISFGTSAVTPDIPLRATYPRTDFALPNQEAKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTVIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLIGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHISRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI DIP protein Q7L1W4 original sequence version: 106 52A8B6EEF6F0FE2B lrrc8d-1 1 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Table 1 lrrc8d-2 2 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Supplementary Fig 1