UniProt UniProt http://www.uniprot.org Molecular Insights into the Architecture of the Human SMC5/6 Complex. Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 2020 imex curation Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Accepted 2021-JUN-29 AT 13:50 BST AT 13:50 BST by MGT Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation Molecular Insights into the Architecture of the Human SMC5/6 Complex. Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 2020 imex curation Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. DSBU (disuccinimidyl dibutyric urea) crosslinker. jpalecek@sci.muni.cz Accepted 2021-JUN-29 AT 13:50 BST AT 13:50 BST by MGT Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation Molecular Insights into the Architecture of the Human SMC5/6 Complex. Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 2020 imex curation Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) tem transmission electron microscopy tem predetermined predetermined participant predetermined Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Accepted 2021-JUN-29 AT 13:50 BST AT 13:50 BST by MGT Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation Molecular Insights into the Architecture of the Human SMC5/6 Complex. Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 2020 imex curation Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Accepted 2021-JUN-29 AT 13:50 BST AT 13:50 BST by MGT Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation Molecular Insights into the Architecture of the Human SMC5/6 Complex. Molecular Insights into the Architecture of the Human SMC5/6 Complex. Journal of molecular biology 2020 imex curation Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. EMSA not curated as they have not fully described the sequence of the ssDNA substrate. jpalecek@sci.muni.cz Accepted 2021-JUN-29 AT 13:50 BST AT 13:50 BST by MGT Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation nse1_human Non-structural maintenance of chromosomes element 1 homolog NSMCE1 HSPC333 HSPC337 protein protein human Homo sapiens Human MQGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH 58A3C2AF70FC8F39 nse2_human E3 SUMO-protein ligase NSE2 NSMCE2 C8orf36 MMS21 Non-structural maintenance of chromosomes element 2 homolog MMS21 homolog E3 SUMO-protein transferase NSE2 protein protein human Homo sapiens Human MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE 24D945C8F16F58F8 slf2_human SMC5-SMC6 complex localization factor protein 2 SLF2 C10orf6 FAM178A Smc5/6 localization factor 1 protein protein human Homo sapiens Human MTRRCMPARPGFPSSPAPGSSPPRCHLRPGSTAHAAAGKRTESPGDRKQSIIDFFKPASKQDRHMLDSPQKSNIKYGGSRLSITGTEQFERKLSSPKESKPKRVPPEKSPIIEAFMKGVKEHHEDHGIHESRRPCLSLASKYLAKGTNIYVPSSYHLPKEMKSLKKKHRSPERRKSLFIHENNEKNDRDRGKTNADSKKQTTVAEADIFNNSSRSLSSRSSLSRHHPEESPLGAKFQLSLASYCRERELKRLRKEQMEQRINSENSFSEASSLSLKSSIERKYKPRQEQRKQNDIIPGKNNLSNVENGHLSRKRSSSDSWEPTSAGSKQNKFPEKRKRNSVDSDLKSTRESMIPKARESFLEKRPDGPHQKEKFIKHIALKTPGDVLRLEDISKEPSDETDGSSAGLAPSNSGNSGHHSTRNSDQIQVAGTKETKMQKPHLPLSQEKSAIKKASNLQKNKTASSTTKEKETKLPLLSRVPSAGSSLVPLNAKNCALPVSKKDKERSSSKECSGHSTESTKHKEHKAKTNKADSNVSSGKISGGPLRSEYGTPTKSPPAALEVVPCIPSPAAPSDKAPSEGESSGNSNAGSSALKRKLRGDFDSDEESLGYNLDSDEEEETLKSLEEIMALNFNQTPAATGKPPALSKGLRSQSSDYTGHVHPGTYTNTLERLVKEMEDTQRLDELQKQLQEDIRQGRGIKSPIRIGEEDSTDDEDGLLEEHKEFLKKFSVTIDAIPDHHPGEEIFNFLNSGKIFNQYTLDLRDSGFIGQSAVEKLILKSGKTDQIFLTTQGFLTSAYHYVQCPVPVLKWLFRMMSVHTDCIVSVQILSTLMEITIRNDTFSDSPVWPWIPSLSDVAAVFFNMGIDFRSLFPLENLQPDFNEDYLVSETQTTSRGKESEDSSYKPIFSTLPETNILNVVKFLGLCTSIHPEGYQDREIMLLILMLFKMSLEKQLKQIPLVDFQSLLINLMKNIRDWNTKVPELCLGINELSSHPHNLLWLVQLVPNWTSRGRQLRQCLSLVIISKLLDEKHEDVPNASNLQVSVLHRYLVQMKPSDLLKKMVLKKKAEQPDGIIDDSLHLELEKQAYYLTYILLHLVGEVSCSHSFSSGQRKHFVLLCGALEKHVKCDIREDARLFYRTKVKDLVARIHGKWQEIIQNCRPTQGQLHDFWVPDS 0A9FB5662185A633 nse3_human Non-structural maintenance of chromosomes element 3 homolog HCA4 Hepatocellular carcinoma-associated protein 4 MAGE-G1 antigen MAGEG1 Melanoma-associated antigen G1 NDNL2 Necdin-like protein 2 NSMCE3 protein protein human Homo sapiens Human MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS C00E2FB2D2CCED7B smc6_human Structural maintenance of chromosomes protein 6 SMC6 SMC6L1 protein protein human Homo sapiens Human MAKRKEENFSSPKNAKRPRQEELEDFDKDGDEDECKGTTLTAAEVGIIESIHLKNFMCHSMLGPFKFGSNVNFVVGNNGSGKSAVLTALIVGLGGRAVATNRGSSLKGFVKDGQNSADISITLRNRGDDAFKASVYGNSILIQQHISIDGSRSYKLKSATGSVVSTRKEELIAILDHFNIQVDNPVSVLTQEMSKQFLQSKNEGDKYKFFMKATQLEQMKEDYSYIMETKERTKEQIHQGEERLTELKRQCVEKEERFQSIAGLSTMKTNLESLKHEMAWAVVNEIEKQLNAIRDNIKIGEDRAARLDRKMEEQQVRLNEAEQKYKDIQDKLEKISEETNARAPECMALKADVVAKKRAYNEAEVLYNRSLNEYKALKKDDEQLCKRIEELKKSTDQSLEPERLERQKKISWLKERVKAFQNQENSVNQEIEQFQQAIEKDKEEHGKIKREELDVKHALSYNQRQLKELKDSKTDRLKRFGPNVPALLEAIDDAYRQGHFTYKPVGPLGACIHLRDPELALAIESCLKGLLQAYCCHNHADERVLQALMKRFYLPGTSRPPIIVSEFRNEIYDVRHRAAYHPDFPTVLTALEIDNAVVANSLIDMRGIETVLLIKNNSVARAVMQSQKPPKNCREAFTADGDQVFAGRYYSSENTRPKFLSRDVDSEISDLENEVENKTAQILNLQQHLSALEKDIKHNEELLKRCQLHYKELKMKIRKNISEIRELENIEEHQSVDIATLEDEAQENKSKMKMVEEHMEQQKENMEHLKSLKIEAENKYDAIKFKINQLSELADPLKDELNLADSEVDNQKRGKRHYEEKQKEHLDTLNKKKRELDMKEKELEEKMSQARQICPERIEVEKSASILDKEINRLRQKIQAEHASHGDREEIMRQYQEARETYLDLDSKVRTLKKFIKLLGEIMEHRFKTYQQFRRCLTLRCKLYFDNLLSQRAYCGKMNFDHKNETLSISVQPGEGNKAAFNDMRALSGGERSFSTVCFILSLWSIAESPFRCLDEFDVYMDMVNRRIAMDLILKMADSQRFRQFILLTPQSMSSLPSSKLIRILRMSDPERGQTTLPFRPVTQEEDDDQR 03C6F478E34285E6 eid3_human EP300-interacting inhibitor of differentiation 3 EID-1-like inhibitor of differentiation 3 E1A-like inhibitor of differentiation 3 EID3 Non-structural maintenance of chromosomes element 4 homolog B protein protein human Homo sapiens Human MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEALEEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLSDCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDLSSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRLPILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY 3B35D08977C47C5A slf1_human SMC5-SMC6 complex localization factor protein 1 SLF1 ANKRD32 Ankyrin repeat domain-containing protein 32 BRCT domain-containing protein 1 BRCTD1 Smc5/6 localization factor 1 protein protein human Homo sapiens Human MEDGTPKHIIQMTGFKMEEKEALVKLLLKLDCTFIKSEKYKNCTHLIAERLCKSEKFLAACAAGKWILTKDYIIHSAKSGRWLDETTYEWGYKIEKDSRYSPQMQSAPKRWREELKRTGAPGAFHRWKVVLLVRTDKRSDSLIRVLEAGKANVILPKSSPSGITHVIASNARIKAEKEKDNFKAPFYPIQYLGDFLLEKEIQNDEDSQTNSVWTEHSNEETNKDFRKDAGFLEMKGALRETMYRTQKEMQNHEDVNVGSILIQHHKKEKFSGSSKDLKFVKMRNTFGSHTYENQKEIKKKDEDIQRSYTLRRKRKKGKESNCKKGVEHEKIKSTLRRHIYNRDQKEMKNSIFAEYAKESKAMAIKTDVDVVEIKNTLRKHIYRAQAVRYNCIRIDKQPVYNVEVKNAEFPRGVLNLIESLIEGHFFKEAIEELSTLQAHYIPPVCVLHALLENVLQDNIDTFSGRYFHILSALLHLHPPWKSPAMSRYYLELFQCPTCMKGAWSLVEVLIRSCLFNESFCHQISENIGSKVLHLTLLKFFFNLIESEVQHLSQKLYDWSDSQNLKITGKAMLLEIFWSGSETSGLLTKPVNMLLEWTIYSHKEKFKSNDVFKHELAYLLAGILGAAIDYWIFLGLKMGRNVMRHMSDDLGSYVSLSCDDFSSQELEIFICSFSSSWLQMFVAEAVFKKLCLQSSGSVSSEPLSLQKMVYSYLPALGKTGVLGSGKIQVSKKIGQRPCFDSQRTLLMLNGTKQKQVEGLPELLDLNLAKCSSSLKKLKKKSEGELSCSKENCPSVVKKMNFHKTNLKGETALHRACINNQVEKLILLLSLPGIDINVKDNAGWTPLHEACNYGNTVCVQEILQRCPEVDLLTQVDGVTPLHDALSNGHVEIGKLLLQHGGPVLLQQRNAKGELPLDYVVSPQIKEELFAITKIEDTVENFHAQAEKHFHYQQLEFGSFLLSRMLLNFCSIFDLSSEFILASKGLTHLNELLMACKSHKETTSVHTDWLLDLYAGNIKTLQKLPHILKELPENLKVCPGVHTEALMITLEMMCRSVMEFS C624008C7CC25FE6 smc5_human Structural maintenance of chromosomes protein 5 SMC5 KIAA0594 SMC5L1 protein protein human Homo sapiens Human MATPSKKTSTPSPQPSKRALPRDPSSEVPSKRKNSAPQLPLLQSSGPFVEGSIVRISMENFLTYDICEVSPGPHLNMIVGANGTGKSSIVCAICLGLAGKPAFMGRADKVGFFVKRGCSRGMVEIELFRASGNLVITREIDVAKNQSFWFINKKSTTQKIVEEKVAALNIQVGNLCQFLPQDKVGEFAKLSKIELLEATEKSIGPPEMHKYHCELKNLREKEKQLETSCKEKTEYLQKMVQRNERYKQDVERFYERKRHLDLIEMLEAKRPWVEYENVRQEYEEVKLVRDRVKEEVRKLKEGQIPVTCRIEEMENERHNLEARIKEKATDIKEASQKCKQKQDVIERKDKHIEELQQALIVKQNEELDRQRRIGNTRKMIEDLQNELKTTENCENLQPQIDAITNDLRRIQDEKALCEGEIIDKRRERETLEKEKKSVDDHIVRFDNLMNQKEDKLRQRFRDTYDAVLWLRNNRDKFKQRVCEPIMLTINMKDNKNAKYIENHIPSNDLRAFVFESQEDMEVFLKEVRDNKKLRVNAVIAPKSSYADKAPSRSLNELKQYGFFSYLRELFDAPDPVMSYLCCQYHIHEVPVGTEKTRERIERVIQETRLKQIYTAEEKYVVKTSFYSNKVISSNTSLKVAQFLTVTVDLEQRRHLEEQLKEIHRKLQAVDSGLIALRETSKHLEHKDNELRQKKKELLERKTKKRQLEQKISSKLGSLKLMEQDTCNLEEEERKASTKIKEINVQKAKLVTELTNLIKICTSLHIQKVDLILQNTTVISEKNKLESDYMAASSQLRLTEQHFIELDENRQRLLQKCKELMKRARQVCNLGAEQTLPQEYQTQVPTIPNGHNSSLPMVFQDLPNTLDEIDALLTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS 54393D50688A914A nsmce1-nsmce3-3 2 9 unspecified role unspecified role neutral component neutral component purified purified lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-262 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component purified purified lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 16 39 17 29 30 18 19 31 33 20 21 34 35 22 36 23 24 37 38 25 32 26 40 27 direct interaction direct interaction Table 1 nsmce1-nsmce3-4 2 11 unspecified role unspecified role neutral component neutral component purified purified lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-194 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-103 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-103 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-102 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-102 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain thr-210 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-120 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-269 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-194 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role neutral component neutral component purified purified lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain thr-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-140 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 43 78 44 79 80 45 81 46 47 117 48 118 119 49 120 50 87 51 86 52 53 88 89 54 90 55 93 56 94 57 71 58 61 99 62 97 63 103 64 100 101 65 66 107 110 67 68 111 69 98 105 70 72 104 108 73 109 74 102 75 112 76 96 77 113 82 114 83 84 115 85 106 121 91 92 116 physical association physical association Table 2 smc5-smc6-7 2 10 unspecified role unspecified role neutral component neutral component purified purified lys-206 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-206 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-628 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-628 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain thr-229 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain thr-233 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-234 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-248 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-248 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-913 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-914 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-310 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain tyr-325 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-379 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain tyr-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified lys-189 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-498 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-542 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-548 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-300 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-332 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-332 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-339 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-746 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ser-717 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ser-717 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-719 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-378 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-710 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-714 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain tyr-499 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-189 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-495 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 151 124 153 125 126 155 127 156 157 128 158 129 159 130 160 131 161 132 133 162 134 163 135 164 136 165 166 137 167 138 168 139 169 140 170 141 171 142 172 143 144 173 174 145 176 146 147 175 177 148 154 149 direct interaction direct interaction Tables 3, 4, 5 eid3-nsmce1-1 3 10 unspecified role unspecified role neutral component neutral component purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component purified purified region strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component purified purified 6 unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig. 4 eid3-smc5-1 4 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 5A nsmce2-smc5-1 4 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association Fig. 5A slf2-smc5-1 4 13 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5A, Supp Fig. 4D slf2-slf1-1 4 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5B slf2-smc6-1 4 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5D slf2-smc6-2 4 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5D slf2-smc5-2 1 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 13 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 5C smc5-slf2-1 1 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 5F smc6-slf2-1 5 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 5E eid3-nsmce1-2 2 10 unspecified role unspecified role neutral component neutral component purified purified lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-111 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined lys-192 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 237 257 250 238 239 251 252 240 241 253 258 242 245 248 256 247 physical association physical association Table 3A