DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for RNA polymerase III transcription repression by Maf1. Structural basis for RNA polymerase III transcription repression by Maf1. Nature structural & molecular biology 2020 imex curation Vorländer MK., Baudin F., Moir RD., Wetzel R., Hagen WJH., Willis IM., Müller CW. christoph.mueller@embl.de 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast pull down pull down predetermined predetermined participant predetermined Vorländer MK., Baudin F., Moir RD., Wetzel R., Hagen WJH., Willis IM., Müller CW. christoph.mueller@embl.de Accepted 2021-APR-14 AT 12:28 BST AT 12:28 BST by MGT Nature structural & molecular biology 2020 Only protein-protein interactions imex curation imex curation Structural basis for RNA polymerase III transcription repression by Maf1. Structural basis for RNA polymerase III transcription repression by Maf1. Nature structural & molecular biology 2020 imex curation Vorländer MK., Baudin F., Moir RD., Wetzel R., Hagen WJH., Willis IM., Müller CW. christoph.mueller@embl.de 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Vorländer MK., Baudin F., Moir RD., Wetzel R., Hagen WJH., Willis IM., Müller CW. Pol III and Maf1-id were incubated together and BS3 crosslinker added to prevent dissociation. christoph.mueller@embl.de Accepted 2021-APR-14 AT 12:28 BST AT 12:28 BST by MGT Nature structural & molecular biology 2020 Only protein-protein interactions imex curation imex curation rpc5_yeast DNA-directed RNA polymerase III subunit RPC5 YKR025W RNA polymerase III subunit C37 DNA-directed RNA polymerase III 37 kDa polypeptide RPC37 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIDNKLFVTEEDEEDRTQDRADVEDESNDIDMIADENGTNSAIANEQEEKSEEVKAEDDTGEEEEDDPVIEEFPLKISGEEESLHVFQYANRPRLVGRKPAEHPFISAARYKPKSHLWEIDIPLDEQAFYNKDKAESEWNGVNVQTLKGVGVENNGQYAAFVKDMQVYLVPIERVAQLKPFFKYIDDANVTRKQEDARRNPNPSSQRAQVVTMSVKSVNDPSQNRLTGSLLAHKVADEEANIELTWAEGTFEQFKDTIVKEAEDKTLVALEKQEDYIDNLV 9E948BDDECE9D15E 9E948BDDECE9D15E rpc3_yeast DNA-directed RNA polymerase III subunit RPC3 RPC82 RPC3 YPR190C P9677.11 DNA-directed III 74 kDa polypeptide C82 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDELLGEALSAENQTGESTVESEKLVTPEDVMTISSLEQRTLNPDLFLYKELVKAHLGERAASVIGMLVALGRLSVRELVEKIDGMDVDSVKTTLVSLTQLRCVKYLQETAISGKKTTYYYYNEEGIHILLYSGLIIDEIITQMRVNDEEEHKQLVAEIVQNVISLGSLTVEDYLSSVTSDSMKYTISSLFVQLCEMGYLIQISKLHYTPIEDLWQFLYEKHYKNIPRNSPLSDLKKRSQAKMNAKTDFAKIINKPNELSQILTVDPKTSLRIVKPTVSLTINLDRFMKGRRSKQLINLAKTRVGSVTAQVYKIALRLTEQKSPKIRDPLTQTGLLQDLEEAKSFQDEAELVEEKTPGLTFNAIDLARHLPAELDLRGSLLSRKPSDNKKRSGSNAAASLPSKKLKTEDGFVIPALPAAVSKSLQESGDTQEEDEEEEDLDADTEDPHSASLINSHLKILASSNFPFLNETKPGVYYVPYSKLMPVLKSSVYEYVIASTLGPSAMRLSRCIRDNKLVSEKIINSTALMKEKDIRSTLASLIRYNSVEIQEVPRTADRSASRAVFLFRCKETHSYNFMRQNLEWNMANLLFKKEKLKQENSTLLKKANRDDVKGRENELLLPSELNQLKMVNERELNVFARLSRLLSLWEVFQMA B39FA74F4A18BADE B39FA74F4A18BADE rpc8_yeast DNA-directed RNA polymerase III subunit RPC8 RPC25 YKL144C YKL1 UNF1 RNA polymerase III subunit C25 DNA-directed RNA polymerase III 25 kDa polypeptide protein protein yeast Saccharomyces cerevisiae Baker's yeast MFILSKIADLVRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSLLGIFDDIFIPQNMLFEGCYYTPEESAWIWPMDEETKLYFDVNEKIRFRIEREVFVDVKPKSPKERELEERAQLENEIEGKNEETPQNEKPPAYALLGSCQTDGMGLVSWWE 7A2422D5B0CEEEC5 7A2422D5B0CEEEC5 rpac2_yeast DNA-directed RNA polymerases I and III subunit RPAC2 YNL113W N1937 RPC19 DNA-directed RNA polymerases I and III 16 kDa polypeptide RPA19 AC19 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTEDIEQKKTATEVTPQEPKHIQEEEEQDVDMTGDEEQEEEPDREKIKLLTQATSEDGTSASFQIVEEDHTLGNALRYVIMKNPDVEFCGYSIPHPSENLLNIRIQTYGETTAVDALQKGLKDLMDLCDVVESKFTEKIKSM 0B048299A787EEDB 0B048299A787EEDB rpc9_yeast DNA-directed RNA polymerase III subunit RPC9 YJL011C J1349 RPC17 RNA polymerase III subunit C17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVLEERNAFLSDYEVLKFLTDLEKKHLWDQKSLAALKKSRSKGKQNRPYNHPELQGITRNVVNYLSINKNFINQEDEGEERESSGAKDAEKSGISKMSDESFAELMTKLNSFKLFKAEKLQIVNQLPANMVHLYSIVEECDARFDEKTIEEMLEIISGYA BA1FF62FE3676C51 BA1FF62FE3676C51 rpac1_yeast DNA-directed RNA polymerases I and III subunit RPAC1 RPC40 YPR110C P8283.18 RPC5 DNA-directed RNA polymerases I and III 40 kDa polypeptide C37 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNIVGIEYNRVTNTTSTDFPGFSKDAENEWNVEKFKKDFEVNISSLDAREANFDLINIDTSIANAFRRIMISEVPSVAAEYVYFFNNTSVIQDEVLAHRIGLVPLKVDPDMLTWVDSNLPDDEKFTDENTIVLSLNVKCTRNPDAPKGSTDPKELYNNAHVYARDLKFEPQGRQSTTFADCPVVPADPDILLAKLRPGQEISLKAHCILGIGGDHAKFSPVSTASYRLLPQINILQPIKGESARRFQKCFPPGVIGIDEGSDEAYVKDARKDTVSREVLRYEEFADKVKLGRVRNHFIFNVESAGAMTPEEIFFKSVRILKNKAEYLKNCPITQ 76A5D6784403E990 76A5D6784403E990 rpab5_yeast DNA-directed RNA polymerases I, II, and III subunit RPABC5 YOR210W RPB10 ABC10-beta ABC8 DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide protein protein yeast Saccharomyces cerevisiae Baker's yeast MIVPVRCFSCGKVVGDKWESYLNLLQEDELDEGTALSRLGLKRYCCRRMILTHVDLIEKFLRYNPLEKRD E1F5733E8F466BE0 E1F5733E8F466BE0 rpab2_yeast DNA-directed RNA polymerases I, II, and III subunit RPABC2 RPO26 RPB6 YPR187W P9677.8 DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide ABC23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDYEEAFNDGNENFEDFDVEHFSDEETYEEKPQFKDGETTDANGKTIVTGGNGPEDFQQHEQIRRKTLKEKAIPKDQRATTPYMTKYERARILGTRALQISMNAPVFVDLEGETDPLRIAMKELAEKKIPLVIRRYLPDGSFEDWSVEELIVDL AA3DEF529F94A5E4 AA3DEF529F94A5E4 rpc6_yeast DNA-directed RNA polymerase III subunit RPC6 RPC34 YNR003C N2031 DNA-directed RNA polymerase III 36 kDa polypeptide C34 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGMIENGLQLSDNAKTLHSQMMSKGIGALFTQQELQKQMGIGSLTDLMSIVQELLDKNLIKLVKQNDELKFQGVLESEAQKKATMSAEEALVYSYIEASGREGIWSKTIKARTNLHQHVVLKCLKSLESQRYVKSVKSVKFPTRKIYMLYSLQPSVDITGGPWFTDGELDIEFINSLLTIVWRFISENTFPNGFKNFENGPKKNVFYAPNVKNYSTTQEILEFITAAQVANVELTPSNIRSLCEVLVYDDKLEKVTHDCYRVTLESILQMNQGEGEPEAGNKALEDEEEFSIFNYFKMFPASKHDKEVVYFDEWTI BDE5CD6FD08BDA7F BDE5CD6FD08BDA7F rpab3_yeast DNA-directed RNA polymerases I, II, and III subunit RPABC3 RPB8 YOR224C YOR50-14 DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide ABC14.5 ABC14.4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNTLFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLNLEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMRLEGNYRNLNNLKQENAYLLIRR 5B71693FE6FD8863 5B71693FE6FD8863 rpc4_yeast DNA-directed RNA polymerase III subunit RPC4 RPC53 RPC4 YDL150W D1557 DNA-directed RNA polymerase III 47 kDa polypeptide C53 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNKGNGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGNDKKHFNNKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSGSSLVQKGLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDDGESEKSSDVDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPSVKTEPVGTGLQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNKPERFMVFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRELAGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKIVVTPQI 206FBAC274BC72D5 206FBAC274BC72D5 rpc10_yeast DNA-directed RNA polymerase III subunit RPC10 RPC11 YDR045C YD9609.01C RNA polymerase III subunit C11 DNA-directed RNA polymerases III 12.5 kDa polypeptide protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSFCPSCNNMLLITSGDSGVYTLACRSCPYEFPIEGIEIYDRKKLPRKEVDDVLGGGWDNVDQTKTQCPNYDTCGGESAYFFQLQIRSADEPMTTFYKCVNCGHRWKEN E4AA802EC9F92230 E4AA802EC9F92230 rpc7_yeast DNA-directed RNA polymerase III subunit RPC7 RPC31 ACP2 RPC8 YNL151C N1769 DNA-directed RNA polymerase III 31 kDa polypeptide protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSYRGGSRGGGSNYMSNLPFGLGYGDVGKNHITEFPSIPLPINGPITNKERSLAVKYINFGKTVKDGPFYTGSMSLIIDQQENSKSGKRKPNIILDEDDTNDGIERYSDKYLKKRKIGISIDDHPYNLNLFPNELYNVMGINKKKLLAISKFNNADDVFTGTGLQDENIGLSMLAKLKELAEDVDDASTGDGAAKGSKTGEGEDDDLADDDFEEDEDEEDDDDYNAEKYFNNGDDDDYGDEEDPNEEAAF 33A7D8EAE47793E1 33A7D8EAE47793E1 rpc2_yeast DNA-directed RNA polymerase III subunit RPC2 YOR207C RET1 RPC2 RPC128 DNA-directed RNA polymerase III 130 kDa polypeptide C128 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAATKRRKTHIHKHVKDEAFDDLLKPVYKGKKLTDEINTAQDKWHLLPAFLKVKGLVKQHLDSFNYFVDTDLKKIIKANQLILSDVDPEFYLKYVDIRVGKKSSSSTKDYLTPPHECRLRDMTYSAPIYVDIEYTRGRNIIMHKDVEIGRMPIMLRSNKCILYDADESKMAKLNECPLDPGGYFIVNGTEKVILVQEQLSKNRIIVEADEKKGIVQASVTSSTHERKSKTYVITKNGKIYLKHNSIAEEIPIAIVLKACGILSDLEIMQLVCGNDSSYQDIFAVNLEESSKLDIYTQQQALEYIGAKVKTMRRQKLTILQEGIEAIATTVIAHLTVEALDFREKALYIAMMTRRVVMAMYNPKMIDDRDYVGNKRLELAGQLISLLFEDLFKKFNNDFKLSIDKVLKKPNRAMEYDALLSINVHSNNITSGLNRAISTGNWSLKRFKMERAGVTHVLSRLSYISALGMMTRISSQFEKSRKVSGPRALQPSQFGMLCTADTPEGEACGLVKNLALMTHITTDDEEEPIKKLCYVLGVEDITLIDSASLHLNYGVYLNGTLIGSIRFPTKFVTQFRHLRRTGKVSEFISIYSNSHQMAVHIATDGGRICRPLIIVSDGQSRVKDIHLRKLLDGELDFDDFLKLGLVEYLDVNEENDSYIALYEKDIVPSMTHLEIEPFTILGAVAGLIPYPHHNQSPRNTYQCAMGKQAIGAIAYNQFKRIDTLLYLMTYPQQPMVKTKTIELIDYDKLPAGQNATVAVMSYSGYDIEDALVLNKSSIDRGFGRCETRRKTTTVLKRYANHTQDIIGGMRVDENGDPIWQHQSLGPDGLGEVGMKVQSGQIYINKSVPTNSADAPNPNNVNVQTQYREAPVIYRGPEPSHIDQVMMSVSDNDQALIKVLLRQNRRPELGDKFSSRHGQKGVCGIIVKQEDMPFNDQGIVPDIIMNPHGFPSRMTVGKMIELISGKAGVLNGTLEYGTCFGGSKLEDMSKILVDQGFNYSGKDMLYSGITGECLQAYIFFGPIYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCVIAYGASQLLLERLMISSDAFEVDVCDKCGLMGYSGWCTTCKSAENIIKMTIPYAAKLLFQELLSMNIAPRLRLEDIFQQ 6AC52354F34CF090 6AC52354F34CF090 maf1_yeast Repressor of RNA polymerase III transcription MAF1 YD8119.11C YDR005C MAF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFIDELDIERVNQTLNFETNDCKIVGSCDIFTTKAVASDRKLYKTIDQHLDTILQENENYNATLQQQLAAPETNQSPCSSPFYSNRRDSNSFWEQKRRISFSEYNSNNNTNNSNGNSSNNNNYSGPNGSSPATFPKSAKLNDQNLKELVSNYDSGSMSSSSLDSSSKNDERIRRRSSSSISSFKSGKSSNNNYSSGTATNNVNKRRKSSINERPSNLSLGPFGPINEPSSRKIFAYLIAILNASYPDHDFSSVEPTDFVKTSLKTFISKFENTLYSLGRQPEEWVWEVINSHMTLSDCVLFQYSPSNSFLEDEPGYLWNLIGFLYNRKRKRVAYLYLICSRLNSSTGEVEDALAKKPQGKLIIDDGSNEYEGEYDFTYDENVIDDKSDQEESLQ 1AE6262D0BEBBB1D 1AE6262D0BEBBB1D rpab4_yeast DNA-directed RNA polymerases I, II, and III subunit RPABC4 RPC10 RPB12 YHR143W-A YHR143BW ABC10-alpha protein protein yeast Saccharomyces cerevisiae Baker's yeast MSREGFQIPTNLDAAAAGTSQARTATLKYICAECSSKLSLSRTDAVRCKDCGHRILLKARTKRLVQFEAR 066A3D982EC7361E 066A3D982EC7361E rpab1_yeast DNA-directed RNA polymerases I, II, and III subunit RPABC1 RPB5 YBR154C YBR1204 RPA7 RPC9 DNA-directed RNA polymerases I, II, and III 27 kDa polypeptide ABC27 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDQENERNISRLWRAFRTVKEMVKDRGYFITQEEVELPLEDFKAKYCDSMGRPQRKMMSFQANPTEESISKFPDMGSLWVEFCDEPSVGVKTMKTFVIHIQEKNFQTGIFVYQNNITPSAMKLVPSIPPATIETFNEAALVVNITHHELVPKHIRLSSDEKRELLKRYRLKESQLPRIQRADPVALYLGLKRGEVVKIIRKSETSGRYASYRICM 2A15F7114D69D829 2A15F7114D69D829 rpc1_yeast DNA-directed RNA polymerase III subunit RPC1 RPO31 RPC160 RPC1 YOR116C O3254 YOR3254C DNA-directed RNA polymerase III largest subunit RNA polymerase III subunit C160 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKEVVVSETPKRIKGLEFSALSAADIVAQSEVEVSTRDLFDLEKDRAPKANGALDPKMGVSSSSLECATCHGNLASCHGHFGHLKLALPVFHIGYFKATIQILQGICKNCSAILLSETDKRQFLHELRRPGVDNLRRMGILKKILDQCKKQRRCLHCGALNGVVKKAAAGAGSAALKIIHDTFRWVGKKSAPEKDIWVGEWKEVLAHNPELERYVKRCMDDLNPLKTLNLFKQIKSADCELLGIDATVPSGRPETYIWRYLPAPPVCIRPSVMMQDSPASNEDDLTVKLTEIVWTSSLIKAGLDKGISINNMMEHWDYLQLTVAMYINSDSVNPAMLPGSSNGGGKVKPIRGFCQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLSIDEVAVPDRVAKVLTYPEKVTRYNRHKLQELIVNGPNVHPGANYLLKRNEDARRNLRYGDRMKLAKNLQIGDVVERHLEDGDVVLFNRQPSLHRLSILSHYAKIRPWRTFRLNECVCTPYNADFDGDEMNLHVPQTEEARAEAINLMGVKNNLLTPKSGEPIIAATQDFITGSYLISHKDSFYDRATLTQLLSMMSDGIEHFDIPPPAIMKPYYLWTGKQVFSLLIKPNHNSPVVINLDAKNKVFVPPKSKSLPNEMSQNDGFVIIRGSQILSGVMDKSVLGDGKKHSVFYTILRDYGPQEAANAMNRMAKLCARFLGNRGFSIGINDVTPADDLKQKKEELVEIAYHKCDELITLFNKGELETQPGCNEEQTLEAKIGGLLSKVREEVGDVCINELDNWNAPLIMATCGSKGSTLNVSQMVAVVGQQIISGNRVPDGFQDRSLPHFPKNSKTPQSKGFVRNSFFSGLSPPEFLFHAISGREGLVDTAVKTAETGYMSRRLMKSLEDLSCQYDNTVRTSANGIVQFTYGGDGLDPLEMEGNAQPVNFNRSWDHAYNITFNNQDKGLLPYAIMETANEILGPLEERLVRYDNSGCLVKREDLNKAEYVDQYDAERDFYHSLREYINGKATALANLRKSRGMLGLLEPPAKELQGIDPDETVPDNVKTSVSQLYRISEKSVRKFLEIALFKYRKARLEPGTAIGAIGAQSIGEPGTQMTLKTFHFAGVASMNVTLGVPRIKEIINASKVISTPIINAVLVNDNDERAARVVKGRVEKTLLSDVAFYVQDVYKDNLSFIQVRIDLGTIDKLQLELTIEDIAVAITRASKLKIQASDVNIIGKDRIAINVFPEGYKAKSISTSAKEPSENDVFYRMQQLRRALPDVVVKGLPDISRAVINIRDDGKRELLVEGYGLRDVMCTDGVIGSRTTTNHVLEVFSVLGIEAARYSIIREINYTMSNHGMSVDPRHIQLLGDVMTYKGEVLGITRFGLSKMRDSVLQLASFEKTTDHLFDAAFYMKKDAVEGVSECIILGQTMSIGTGSFKVVKGTNISEKDLVPKRCLFESLSNEAALKAN D1697EB2352BCA4F D1697EB2352BCA4F maf1-ret1-1 2 17 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 15 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 11 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 4 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 13 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 18 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 6 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 9 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 14 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 12 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 5 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 10 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 19 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 7 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 8 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 16 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast 20 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified yeast Saccharomyces cerevisiae Baker's yeast physical association physical association Table S1 rpc34-maf1-1 1 11 unspecified role unspecified role bait bait purified purified n-terminus His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey purified purified P41910:p.[Asp248Ala;Asp250Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain D A Thu Apr 22 11:28:25 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp248Ala;Asp250Ala]' P41910:p.Thr33_Thr34delinsGluGlu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain TT EE Thu Apr 22 13:11:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr33_Thr34delinsGluGLu' P41910:p.Leu318_Trp319delinsSerSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LW SS Thu Apr 22 13:28:02 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu318_Trp319delinsSerSer' ecoli Escherichia coli (strain K12) direct interaction direct interaction 2B