IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Antiviral research 2020 imex curation Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus amesecar@purdue.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel weight by staining molecular weight estimation by staining weight by staining Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins amesecar@purdue.edu Accepted 2021-MAY-03 AT 08:47 BST AT 08:47 BST by MARTA Antiviral research 2020 Only protein-protein interactions imex curation imex curation Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Antiviral research 2020 imex curation Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus amesecar@purdue.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins ISG15-AMC is stated to have been suppled by "LifeSensors", only no such product can be traced on their website. For H1652R, (aa799) V1691R, and V1691K (aa838) mutants I have entered as "mutation disrupting" for ubiq - as although I cannot see any orange band, the assays have been carried out as text states:- "which were initiated with a higher enzyme concentration of 0.83 μM (final concentration) due to lower turnover rates in cleaving both substrates which was initially observed". PEPTIDE:I have entered the mutations as increasing/decreasing if results are greater than midway between +/- 100 and 150% of Relative Activity of WT. This would give a result of >+/- ~125% of WT. The authors are stating that an increase in activity is >+/- 150% of WT, although this may refer to proteins only - presumably as this would support their various hypotheses. The increase in AMC fluorescence was observed after the cleavage of AMC from tagged molecules. amesecar@purdue.edu Accepted 2021-MAY-03 AT 08:47 BST AT 08:47 BST by MARTA Antiviral research 2020 Only protein-protein interactions imex curation imex curation Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Antiviral research 2020 imex curation Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus amesecar@purdue.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins amesecar@purdue.edu Accepted 2021-MAY-03 AT 08:47 BST AT 08:47 BST by MARTA Antiviral research 2020 Only protein-protein interactions imex curation imex curation Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease. Antiviral research 2020 imex curation Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus amesecar@purdue.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro probe interaction assay probe interaction assay probe interaction assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Clasman JR., Everett RK., Srinivasan K., Mesecar AD., Clasman JR., Everett RK., Srinivasan K., Mesecar AD. Virus - Publications including interactions involving viral proteins The PA group reacts with the catalytic cysteine of PLpro to form a covalent complex. amesecar@purdue.edu Accepted 2021-MAY-03 AT 08:47 BST AT 08:47 BST by MARTA Antiviral research 2020 Only protein-protein interactions imex curation imex curation rlrgg RLRGG peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) k9n7c7-pro_0000422441 Papain-like proteinase rep ORF1ab polyprotein 1a-1b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC APVKKVAFGGDQVHEVAAVRSVTVEYNIHAVLDTLLASSSLRTFVVDKSLSIEEFADVVKEQVSDLLVKLLRGMPIPDFDLDDFIDAPCYCFNAEGDASWSSTMIFSLHPVECDEECSEVEASDLEEGESECISETSTEQVDVSHEISDDEWAAAVDEAFPLDEAEDVTESVQEEAQPVEVPVEDIAQVVIADTLQETPVVSDTVEVPPQVVKLPSEPQTIQPEVKEVAPVYEADTEQTQSVTVKPKRLRKKRNVDPLSNFEHKVITECVTIVLGDAIQVAKCYGESVLVNAANTHLKHGGGIAGAINAASKGAVQKESDEYILAKGPLQVGDSVLLQGHSLAKNILHVVGPDARAKQDVSLLSKCYKAMNAYPLVVTPLVSAGIFGVKPAVSFDYLIREAKTRVLVVVNSQDVYKSLTIVDIPQSLTFSYDGLRGAIRKAKDYGFTVFVCTDNSANTKVLRNKGVDYTKKFLTVDGVQYYCYTSKDTLDDILQQANKSVGIISMPLGYVSHGLDLIQAGSVVRRVNVPYVCLLANKEQEAILMSEDVKLNPSEDFIKHVRTNGGYNSWHLVEGELLVQDLRLNKLLHWSDQTICYKDSVFYVVKNSTAFPFETLSACRAYLDSRTTQQLTIEVLVTVDGVNFRTVVLNNKNTYRSQLGCVFFNGADISDTIPDEKQNGHSLYLADNLTADETKALKELYGPVDPTFLHRFYSLKAAVHKWKMVVCDKVRSLKLSDNNCYLNAVIMTLDLLKDIKFVIPALQHAFMKHKGGDSTDFIALIMAYGNCTFGAPDDASRLLHTVLAKAELCCSARMVWREWCNVCGIKDVVLQGLKACCYVGVQTVEDLRARMTYVCQCGGERHRQIVEHTTPWLLLSGTPNEKLVTTSTAPDFVAFNVFQGIETAVGHYVHARLKGGLILKFDSGTVSKTSDWKCKVTDVLFPGQKYSSDCNVVRYSLDGNFRTEVDPDLSAFYVKDGKYFTSEPPVTYSPATILAGSVYTNSCLVSSDGQPGGDAISLSFNNLLGFDSSKPVTKKYTYSFLPKEDGDVLLAEFDTYDPIYKNGAMYKGKPILWVNKASYDTNLNKFNRASLRQIFDVAPIELENKFTPLSVESTPVEPPTVDVVALQQEMTIVKCKGLNKPFVKDNVSFVADDSGTPVVEYLSKEDLHTLYVDPKYQVIVLKDNVLSSMLRLHTVESGDINVVAASGSLTRKVKLLFRASFYFKEFATRTFTATTAVGSCIKSVVRHLGVTKGILTGCFSFVKMLFMLPLAYFSDSKLGTTEVKVSALKTAGVVTGNVVKQCCTAAVDLSMDKLRRVDWKSTLRLLLMLCTTMVLLSSVYHLYVFNQVLSSDVMFEDAQGLKKFYKEVRAYLGISSACDGLASAYRANSFDVPTFCANRSAMCNWCLISQDSITHYPALKMVQTHLSHYVLNIDWLWFAFETGLAYMLYTSAFNWLLLAGTLHYFFAQTSIFVDWRSYNYAVSSAFWLFTHIPMAGLVRMYNLLACLWLLRKFYQHVINGCKDTACLLCYKRNRLTRVEASTVVCGGKRTFYITANGGISFCRRHNWNCVDCDTAGVGNTFICEEVANDLTTALRRPINATDRSHYYVDSVTVKETVVQFNYRRDGQPFYERFPLCAFTNLDKLKFKEVCKTTTGIPEYNFIIYDSSDRGQESLARSACVYYSQVLCKSILLVDSSLVTSVGDSSEIATKMFDSFVNSFVSLYNVTRDKLEKLISTARDGVRRGDNFHSVLTTFIDAARGPAGVESDVETNEIVDSVQYAHKHDIQITNESYNNYVPSYVKPDSVSTSDLGSLIDCNAASVNQIVLRNSNGACIWNAAAYMKLSDALKRQIRIACRKCNLAFRLTTSKLRANDNILSVRFTANKIVGG 854 2740 532E0D4BDE146A6E ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG isg15_human Ubiquitin-like protein ISG15 ISG15 G1P2 UCRP Interferon-induced 15 kDa protein Interferon-induced 17 kDa protein Ubiquitin cross-reactive protein protein protein human Homo sapiens Human MGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRVPLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQDDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGGGTEPGGRS DIP protein P05161 original sequence version: 130 DIP protein P05161 original sequence version: 142 DIP protein P05161 original sequence version: 155 DIP protein P05161 original sequence version: 96 DIP protein P05161 original sequence version: 141 DIP protein P05161 original sequence version: 168 DIP protein P05161 original sequence version: 150 B6858A15AB0FFFDE isg15-rep-24 3 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P05161:p.Cys78Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:29:33 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys78Ser' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 rep-rlrgg-3 2 6 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K9N7C7-PRO_0000422441:p.Lys697Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Mon Mar 01 11:01:04 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Ala' K9N7C7-PRO_0000422441:p.Lys697Phe mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain K F Mon Mar 01 11:18:29 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Phe' K9N7C7-PRO_0000422441:p.Arg796Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R A K9N7C7-PRO_0000422441:p.Arg796Glu mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R E Mon Mar 01 11:05:49 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg796Glu' K9N7C7-PRO_0000422441:p.His799Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Mon Mar 01 11:29:54 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Ala' K9N7C7-PRO_0000422441:p.His799Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H R Mon Mar 01 11:35:22 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Arg' K9N7C7-PRO_0000422441:p.His799Phe mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H F Mon Mar 01 10:52:03 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Phe' K9N7C7-PRO_0000422441:p.Thr800Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T R Mon Mar 01 11:18:58 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Arg' K9N7C7-PRO_0000422441:p.Thr800Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T F Mon Mar 01 11:05:48 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Phe' K9N7C7-PRO_0000422441:p.Val838Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V R Mon Mar 01 11:05:40 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Arg' K9N7C7-PRO_0000422441:p.Val838Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V K Mon Mar 01 11:06:05 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Lys' K9N7C7-PRO_0000422441:p.Val838Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V F Mon Mar 01 11:40:52 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Phe' K9N7C7-PRO_0000422441:p.Val838Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V S Mon Mar 01 11:21:48 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Ser' ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate neutral component neutral component purified purified C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range benzyloxycarbonyl modarg modified L-arginine residue ModArg n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage Figure 4 rep-ubiq_human-4 2 6 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K9N7C7-PRO_0000422441:p.Lys697Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Mon Mar 01 11:01:04 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Ala' K9N7C7-PRO_0000422441:p.Lys697Phe mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain K F Mon Mar 01 11:09:39 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Phe' K9N7C7-PRO_0000422441:p.Arg796Ala mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R A K9N7C7-PRO_0000422441:p.Arg796Glu mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain R E Mon Mar 01 11:34:39 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg796Glu' K9N7C7-PRO_0000422441:p.His799Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Mon Mar 01 11:30:13 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Ala' K9N7C7-PRO_0000422441:p.His799Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain H R Mon Mar 01 11:20:16 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Arg' K9N7C7-PRO_0000422441:p.His799Phe mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H F Mon Mar 01 11:39:26 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Phe' K9N7C7-PRO_0000422441:p.Thr800Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain T R Mon Mar 01 11:29:28 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Arg' K9N7C7-PRO_0000422441:p.Thr800Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T F Mon Mar 01 11:31:12 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Phe' K9N7C7-PRO_0000422441:p.Val838Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V R Mon Mar 01 11:40:11 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Arg' K9N7C7-PRO_0000422441:p.Val838Lys mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V K Mon Mar 01 11:16:20 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Lys' K9N7C7-PRO_0000422441:p.Val838Phe mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain V F Mon Mar 01 10:45:47 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Phe' K9N7C7-PRO_0000422441:p.Val838Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V S Mon Mar 01 10:48:28 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Ser' ecoli Escherichia coli (strain K12) 7 enzyme target enzyme target substrate substrate neutral component neutral component purified purified C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) cleavage cleavage reaction cleavage Figure 4, Table 2 isg15-rep-25 2 6 enzyme enzyme neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K9N7C7-PRO_0000422441:p.Lys697Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Mon Mar 01 11:31:04 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Ala' K9N7C7-PRO_0000422441:p.Lys697Phe mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain K F Mon Mar 01 11:07:31 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys697Phe' K9N7C7-PRO_0000422441:p.Arg796Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A K9N7C7-PRO_0000422441:p.Arg796Glu mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R E Mon Mar 01 10:50:27 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg796Glu' K9N7C7-PRO_0000422441:p.His799Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Mon Mar 01 11:39:10 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Ala' K9N7C7-PRO_0000422441:p.His799Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain H R Mon Mar 01 11:05:31 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Arg' K9N7C7-PRO_0000422441:p.His799Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H F Mon Mar 01 10:43:41 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Phe' K9N7C7-PRO_0000422441:p.Thr800Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T R Mon Mar 01 11:31:38 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Arg' K9N7C7-PRO_0000422441:p.Thr800Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T F Mon Mar 01 10:47:48 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr800Phe' K9N7C7-PRO_0000422441:p.Val838Arg mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V R Mon Mar 01 11:51:20 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Arg' K9N7C7-PRO_0000422441:p.Val838Lys mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain V K Mon Mar 01 10:55:15 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Lys' K9N7C7-PRO_0000422441:p.Val838Phe mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V F Mon Mar 01 11:51:07 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Phe' K9N7C7-PRO_0000422441:p.Val838Ser mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V S Mon Mar 01 10:46:41 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Ser' ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component purified purified C-terminus coumarin label coumarin label c-term range C-terminal range c-term range c-term range C-terminal range c-term range cleavage cleavage reaction cleavage Figure 4, Table 2 isg15-rep-26 4 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P05161:p.Cys78Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 11:03:20 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys78Ser' propargylamide modification modgly modified glycine residue ModGly c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K9N7C7-PRO_0000422441:p.His799Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H R Mon Mar 01 10:47:53 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Arg' K9N7C7-PRO_0000422441:p.Val838Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V R Mon Mar 01 11:34:34 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Arg' K9N7C7-PRO_0000422441:p.Val838Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V K Mon Mar 01 11:20:39 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Lys' ecoli Escherichia coli (strain K12) covalent binding covalent binding Figure 5 rep-ubiq_human-3 4 7 unspecified role unspecified role neutral component neutral component purified purified propargylamide modification modgly modified glycine residue ModGly c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K9N7C7-PRO_0000422441:p.His799Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain H R Mon Mar 01 11:07:52 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His799Arg' K9N7C7-PRO_0000422441:p.Val838Arg mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V R Mon Mar 01 11:20:28 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Arg' K9N7C7-PRO_0000422441:p.Val838Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain V K Mon Mar 01 11:51:15 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val838Lys' ecoli Escherichia coli (strain K12) covalent binding covalent binding Figure 5 isg15-rep-27 1 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P05161:p.Cys78Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Mon Mar 01 10:55:42 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys78Ser' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) covalent binding covalent binding Figure S1