DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. eLife 2019 imex curation Walter JD., Sawicka M., Dutzler R., Walter JD., Sawicka M., Dutzler R. dutzler@bioc.uzh.ch Only protein-protein interactions imex curation human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Walter JD., Sawicka M., Dutzler R., Walter JD., Sawicka M., Dutzler R. dutzler@bioc.uzh.ch Accepted 2021-OCT-07 AT 11:42 BST AT 11:42 BST by MGT eLife 2019 Only protein-protein interactions imex curation imex curation Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport. eLife 2019 imex curation Walter JD., Sawicka M., Dutzler R., Walter JD., Sawicka M., Dutzler R. dutzler@bioc.uzh.ch Only protein-protein interactions imex curation human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Walter JD., Sawicka M., Dutzler R., Walter JD., Sawicka M., Dutzler R. dutzler@bioc.uzh.ch Accepted 2021-OCT-07 AT 11:42 BST AT 11:42 BST by MGT eLife 2019 Only protein-protein interactions imex curation imex curation s26a9_mouse Solute carrier family 26 member 9 Slc26a9 Anion transporter/exchanger protein 9 protein protein mouse Mus musculus Mouse MNQPRPRYVVDRAAYSLSLFDDEFEKKDRAYPVGEKLRNTFRCSSAKFKAFVFGLLPVLSWLPKYKIKDYIIPDLLGGLSGGCIQVPQGMAFALLANLPAVNGLYSSFFPLLTYFFLGGIHQMVPGTFAVISILVGNICLQLAPESKFQIFNNVTNETYVDTAAMEAERLHVSATLACLTAVIQMALGFMQFGFVAIYLSESFIRGFMTAAGLQILISVLKYIFGLTIPSYTGPGSIVFTFIDICKNLPHTNIASLIFALVSGVFLVLVKELNARYMHKIHFPIPTEMIVVVVATAISGSCKMPKKYHMQIVGEIRQGFPTPVAPMVSQWKGMVGTAFSLAIVGYVINLAMGRTLASKHGYDVDSNQEMIALGCSNFFGSFFKIHVICCALSVTLAVDGAGGKSQVASLCVSLVVMITMLVLGSYLYPLPKAVLGALIAVNLKNSLKQLTDPYYLWRKSKLDCCVWVVSFLSSFFLSLPYGVAVGVAFSILVVIFQTQFRNGSTLAQVMDTDIYVNPKTYNRAQEIAGVKIVTYCSPLYLANSEIFRQKVIAKTGMDPQKVLLAKQKYLRKQEKRTAIPTQQRKSLFMKTKTVSLQELQQDFESAPSTDPNNNQAPAAEAHISYITFSPDASTAAACELPASTRSPQEASDTLASVPPFVTFHTLILDMSGVSFVDLMGIKALAKLSSTYEKIGVQIFLVNIHAQVYNDISHGGVFEDGCVQRSHVFPSIHDAVLFAQANAREAPDRNFHGAPGDTEFSLYDSEEEGPSYWDLEQEMFGTMFHTETLTAL 4D473330D4D94DCD slc26a9-1 2 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 direct interaction direct interaction Table 1 slc26a9-4 1 3 unspecified role unspecified role neutral component neutral component purified purified regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 direct interaction direct interaction Figure 1—figure supplement 2C