DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cryo-EM structure of OSCA1.2 from <i>Oryza sativa</i> elucidates the mechanical basis of potential membrane hyperosmolality gating. Cryo-EM structure of OSCA1.2 from <i>Oryza sativa</i> elucidates the mechanical basis of potential membrane hyperosmolality gating. Proceedings of the National Academy of Sciences of the United States of America 2019 imex curation Maity K., Heumann JM., McGrath AP., Kopcho NJ., Hsu PK., Lee CW., Mapes JH., Garza D., Krishnan S., Morgan GP., Hendargo KJ., Klose T., Rees SD., Medrano-Soto A., Saier MH., Piñeros M., Komives EA., Schroeder JI., Chang G., Stowell MHB. stowell@colorado.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Maity K., Heumann JM., McGrath AP., Kopcho NJ., Hsu PK., Lee CW., Mapes JH., Garza D., Krishnan S., Morgan GP., Hendargo KJ., Klose T., Rees SD., Medrano-Soto A., Saier MH., Piñeros M., Komives EA., Schroeder JI., Chang G., Stowell MHB. stowell@colorado.edu Accepted 2021-APR-21 AT 15:55 BST AT 15:55 BST by MGT Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation Cryo-EM structure of OSCA1.2 from <i>Oryza sativa</i> elucidates the mechanical basis of potential membrane hyperosmolality gating. Cryo-EM structure of OSCA1.2 from <i>Oryza sativa</i> elucidates the mechanical basis of potential membrane hyperosmolality gating. Proceedings of the National Academy of Sciences of the United States of America 2019 imex curation Maity K., Heumann JM., McGrath AP., Kopcho NJ., Hsu PK., Lee CW., Mapes JH., Garza D., Krishnan S., Morgan GP., Hendargo KJ., Klose T., Rees SD., Medrano-Soto A., Saier MH., Piñeros M., Komives EA., Schroeder JI., Chang G., Stowell MHB. stowell@colorado.edu 24-May-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation picpa Pichia pastoris (Yeast) Yeast Pichia pastoris molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Maity K., Heumann JM., McGrath AP., Kopcho NJ., Hsu PK., Lee CW., Mapes JH., Garza D., Krishnan S., Morgan GP., Hendargo KJ., Klose T., Rees SD., Medrano-Soto A., Saier MH., Piñeros M., Komives EA., Schroeder JI., Chang G., Stowell MHB. stowell@colorado.edu Accepted 2021-APR-21 AT 15:55 BST AT 15:55 BST by MGT Proceedings of the National Academy of Sciences of the United States of America 2019 Only protein-protein interactions imex curation imex curation q5tkg1_orysj OSNPB_050594700 Os05g0594700 protein protein orysj Oryza sativa subsp. japonica (Rice) Rice MATVSDIGLSAAINVSMAVAFLLVFAFLRLQPINDRVYFPKWYLRGMRDSPVSSGAAVQKVVNLNMRSYLKFLSWMPAALKMPEDELINHAGLDSAVYLRIYLTGIKIFVPISILASLVLFPVNWTNDTLDSMKVVHSKIDKLSISNIPYGSNRFVTHLVMAYAVTFWTCYVLFREYEIITTMRLRFLASEKRRPDQFTVLVRNIPPDPDESISELVEHFFLVNHPDHYLRHQVVYNANKLADLVEKKKKLQNWLDYYQLKYERNPSKRPTTKTGFLGCFGSEVDAIEYYKAEIEKIGKEEADERQKIMKDPQSAVPAAFVSFRSRWGAAVCAQTQQTSNPTVWITEWAPEPRDVYWNNLSIPFVSLTVRRLIVAVAFFFLNFFYVIPIAFVQSLASLEGIEKALPFLKPLIKIDVIKSFIQGFLPGIALKVFLILLPTILMFMSKFEGLISQSSLERRSASKYYIFLFFNVFLGSIVTGSALDQLKAYIHQSANEIPRTIGVAIPMRATFFITYVMVDGWTGVAGEILRLRALIIFHLKNFFLVKTEKDREEAMDPGSICFDWCEPRIQLYFLLGLVYAVVTPLLLPFILVFFGLAYVVYRHQIINVYNQQYESGAQFWPSVHGRIIIALIVSQLLLIGLLSTKGFEETTPVLVVLPVLTFWFYKYCKNRFEPAFVRNPLQEAMRKDTLERAREPTFDLKAYLANAYLHPVFKGREEEDNMSISEDVGMEEVIVPTKRQSRRNTPAQSKYEGSDTLSLPETVHER 74E2967BAD837840 q5tkg1_orysj-1 1 3 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction Table 1 q5tkg1_orysj-q5tkg1_orysj-1 2 3 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Fig S1