DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science (New York, N.Y.) 2019 imex curation Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Only protein-protein interactions imex curation rat-brain Rattus norvegicus brain brain Brain [cerebrum] 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single structure determination identification by structure determination identification by structure determination structure determination Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Accepted 2021-SEP-24 AT 07:55 BST AT 07:55 BST by KALPANAP Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation IMEx MIMIx imex curation Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM. Science (New York, N.Y.) 2019 imex curation Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Only protein-protein interactions imex curation rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Accepted 2021-SEP-24 AT 07:55 BST AT 07:55 BST by KALPANAP Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation p19490-2 Glutamate receptor 1 Gria1 Glur1 AMPA-selective glutamate receptor 1 GluR-A GluR-K1 Glutamate receptor ionotropic, AMPA 1 protein protein rat Rattus norvegicus (Rat) Rat MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL 5FA3091114C0BA6B p19491-2 Glutamate receptor 2 Gria2 Glur2 AMPA-selective glutamate receptor 2 GluR-B GluR-K2 Glutamate receptor ionotropic, AMPA 2 protein protein rat Rattus norvegicus (Rat) Rat MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI DIP protein P19491-2 original sequence version: 144 DIP protein P19491-2 original sequence version: 177 DIP protein P19491-2 original sequence version: 170 DIP protein P19491-2 original sequence version: 186 ccg2_rat Voltage-dependent calcium channel gamma-2 subunit Cacng2 Neuronal voltage-gated calcium channel gamma-2 subunit Transmembrane AMPAR regulatory protein gamma-2 Stargazin Stg protein protein rat Rattus norvegicus (Rat) Rat MGLFDRGVQMLLTTVGAFAAFSLMTIAVGTDYWLYSRGVCKTKSVSENETSKKNEEVMTHSGLWRTCCLEGNFKGLCKQIDHFPEDADYEADTAEYFLRAVRASSIFPILSVILLFMGGLCIAASEFYKTRHNIILSAGIFFVSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALSFIIAEMVGVLAVHMFIDRHKQLRATARATDYLQASAITRIPSYRYRYQRRSRSSSRSTEPSHSRDASPVGVKGFNTLPSTEISMYTLSRDPLKAATTPTATYNSDRDNSFLQVHNCIQKDSKDSLHANTANRRTTPV DIP protein Q71RJ2 original sequence version: 44 DIP protein Q71RJ2 original sequence version: 53 mint AA9D475606A0FBA4 p19492-2 Glutamate receptor 3 Gria3 Glur3 AMPA-selective glutamate receptor 3 GluR-C GluR-K3 Glutamate receptor ionotropic, AMPA 3 protein protein rat Rattus norvegicus (Rat) Rat MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLSYYKWEKFVYLYDTERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMVQQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSSSSENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALGTPVNLAVLKLSEQGILDKLKNKWWYDKGECGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI DIP protein P19492-2 original sequence version: 158 ccg8_rat Voltage-dependent calcium channel gamma-8 subunit Cacng8 Neuronal voltage-gated calcium channel gamma-8 subunit Transmembrane AMPAR regulatory protein gamma-8 protein protein rat Rattus norvegicus (Rat) Rat MESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGSGSSEKKDPGGLTHSGLWRICCLEGLKRGVCVKINHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLLGGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGLSFILAEVIGVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSSSRGSSEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGVGAYGGAAGAAGGGGTGSERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPPAPAAPAPGTLSKEAAASNTNTLNRKTTPV 0FDB1461BDC780A9 gria1-8 2 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] 7 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] 3 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain S A Mon Jun 28 17:15:33 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19490-2:p.[Arg757Gly,Gly758Ala,Ser768Ala,Val772Ala,Ser778Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:07:15 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 6 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] association association Figure 2, Figure 6B gria1-gria2-1 1 5 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] 3 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain S A Mon Jun 28 16:52:59 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19490-2:p.[Arg757Gly,Gly758Ala,Ser768Ala,Val772Ala,Ser778Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:06:17 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, S3, Figure 2A gria1-gria2-2 1 3 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain S A Mon Jun 28 16:57:39 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19490-2:p.[Arg757Gly,Gly758Ala,Ser768Ala,Val772Ala,Ser778Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 16:55:08 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, Figure 2B gria1-gria2-3 1 5 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:08:14 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 6 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, Figure 2C, 3C, 3D gria2-gria3-5 1 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:10:46 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 6 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, S3, Figure 2D gria1-gria2-4 1 5 structure determination identification by structure determination identification by structure determination structure determination experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] 3 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain G A certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain S A Mon Jun 28 17:12:27 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19490-2:p.[Arg757Gly,Gly758Ala,Ser768Ala,Val772Ala,Ser778Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain T A Mon Jun 28 17:41:38 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] 6 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, Figure 2E gria2-23 1 4 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain V A Mon Jun 28 17:24:45 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p19491-2:p.[Thr765Ala,Pro766A,Ser775Ala,Val779Ala]' rat-brain Rattus norvegicus brain brain Brain [cerebrum] direct interaction direct interaction Table S1, Figure 2F