<?xml version='1.0' encoding='UTF-8'?>
<entrySet xmlns="http://psi.hupo.org/mi/mif300" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://psi.hupo.org/mi/mif300 https://raw.githubusercontent.com/HUPO-PSI/miXML/master/3.0/src/MIF300.xsd" level="3" version="0" minorVersion="0">
  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>The first transmembrane region of complement component-9 acts as a brake on its self-assembly.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="30111885" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-28178970" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-29374" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">The first transmembrane region of complement component-9 acts as a brake on its self-assembly.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
            <attribute name="publication year" nameAc="MI:0886">2018</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Spicer BA., Law RHP., Caradoc-Davies TT., Ekkel SM., Bayly-Jones C., Pang SS., Conroy PJ., Ramm G., Radjainia M., Venugopal H., Whisstock JC., Dunstone MA.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">Michelle.Dunstone@monash.edu</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-29374" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="30111885" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9606">
            <names>
              <shortLabel>human-293f</shortLabel>
              <fullName>Homo sapiens HEK293F cell line</fullName>
            </names>
            <cellType>
              <names>
                <shortLabel>293f</shortLabel>
                <fullName>embryonic kidney cell 293f</fullName>
              </names>
              <xref>
                <primaryRef db="intact" dbAc="MI:0469" id="IA:2484" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="mint" dbAc="MI:0471" id="MINT-4303051" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6922069" refType="identity" refTypeAc="MI:0356"/>
              </xref>
            </cellType>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>3d-em</shortLabel>
            <fullName>3D electron microscopy</fullName>
            <alias type="synonym" typeAc="MI:1041">3D-EM</alias>
            <alias type="synonym" typeAc="MI:1041">electron tomog</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0410" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608522" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="12160704" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>experimental particp</shortLabel>
            <fullName>experimental participant identification</fullName>
            <alias type="synonym" typeAc="MI:1041">experimental particp</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0661" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-933978" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Spicer BA., Law RHP., Caradoc-Davies TT., Ekkel SM., Bayly-Jones C., Pang SS., Conroy PJ., Ramm G., Radjainia M., Venugopal H., Whisstock JC., Dunstone MA.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">Michelle.Dunstone@monash.edu</attribute>
          <attribute name="accepted">Accepted 2021-DEC-02 AT 10:38 GMT AT 10:38 GMT by MGT</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature communications</attribute>
          <attribute name="publication year" nameAc="MI:0886">2018</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>pro_0000023602</shortLabel>
          <fullName>Complement component C9</fullName>
          <alias type="gene name" typeAc="MI:0301">C9</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P02748-PRO_0000023602" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-22117467" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P02748" refType="chain-parent" refTypeAc="MI:0951"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>QYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK</sequence>
        <attributeList>
          <attribute name="crc64">B96F7BCA3DB95987</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-29374-1">
        <names>
          <shortLabel>c9-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="wwpdb" dbAc="MI:0805" id="6DLW" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="emdb" dbAc="MI:0936" id="EMD-7773" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-28389632" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-29374-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>structure determination</shortLabel>
                  <fullName>identification by structure determination</fullName>
                  <alias type="synonym" typeAc="MI:1041">identification by structure determination</alias>
                  <alias type="synonym" typeAc="MI:1041">structure determination</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:2287" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-20597534" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="7877166" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>experimental particp</shortLabel>
                  <fullName>experimental participant identification</fullName>
                  <alias type="synonym" typeAc="MI:1041">experimental particp</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0661" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-933978" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human-293f</shortLabel>
                  <fullName>Homo sapiens HEK293F cell line</fullName>
                </names>
                <cellType>
                  <names>
                    <shortLabel>293f</shortLabel>
                    <fullName>embryonic kidney cell 293f</fullName>
                  </names>
                  <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="IA:2484" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="MINT-4303051" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6922069" refType="identity" refTypeAc="MI:0356"/>
                  </xref>
                </cellType>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="22"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">Supplementary Table 6</attribute>
          <attribute name="comment" nameAc="MI:0612">The C9 protein is stated to have an N-terminal Ig-kappa leader sequence and may also have C-terminal tags from the pSectag2a vector. These tags are not stated to have been removed.</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>