MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Scientific reports 2018 imex curation Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR., Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Only protein-protein interactions imex curation mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Accepted 2021-MAY-07 AT 07:20 BST AT 07:20 BST by ORCHARD Scientific reports 2018 Only protein-protein interactions imex curation imex curation Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Scientific reports 2018 imex curation Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR., Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Only protein-protein interactions imex curation mouse-hippocampus Mouse hippocampal neurons hippocampus Hippocampus [Brain hippocampus] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Accepted 2021-MAY-07 AT 07:20 BST AT 07:20 BST by ORCHARD Scientific reports 2018 Only protein-protein interactions imex curation imex curation Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Scientific reports 2018 imex curation Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR., Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Only protein-protein interactions imex curation human-sk-n-sh Homo sapiens, SK-N-SH Caucasian Neuroblastoma Cell Line sk-n-sh sk-n-sh anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Accepted 2021-MAY-07 AT 07:20 BST AT 07:20 BST by ORCHARD Scientific reports 2018 Only protein-protein interactions imex curation imex curation Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Scientific reports 2018 imex curation Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR., Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Only protein-protein interactions imex curation human-sk-n-sh Homo sapiens, SK-N-SH Caucasian Neuroblastoma Cell Line sk-n-sh sk-n-sh pull down pull down anti tag western anti tag western blot anti tag western Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Accepted 2021-MAY-07 AT 07:20 BST AT 07:20 BST by ORCHARD Scientific reports 2018 Only protein-protein interactions imex curation imex curation Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Loss of MeCP2 in adult 5-HT neurons induces 5-HT1A autoreceptors, with opposite sex-dependent anxiety and depression phenotypes. Scientific reports 2018 imex curation Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR., Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Only protein-protein interactions imex curation human-sk-n-sh Homo sapiens, SK-N-SH Caucasian Neuroblastoma Cell Line sk-n-sh sk-n-sh anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Philippe TJ., Vahid-Ansari F., Donaldson ZR., Le François B., Zahrai A., Turcotte-Cardin V., Daigle M., James J., Hen R., Merali Z., Albert PR. Brain Disease - Neurological Diseases & Disorders palbert@uottawa.ca Accepted 2021-MAY-07 AT 07:20 BST AT 07:20 BST by ORCHARD Scientific reports 2018 Only protein-protein interactions imex curation imex curation mecp2_human Methyl-CpG-binding protein 2 MECP2 protein protein human Homo sapiens Human MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS DIP protein P51608 original sequence version: 182 DIP protein P51608-1 original sequence version: 195 EB6A33233AEDA566 deaf1_mouse Deformed epidermal autoregulatory factor 1 homolog Deaf1 Nuclear DEAF-1-related transcriptional regulator protein protein mouse Mus musculus Mouse MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAESEAEEPVLSRDEDSEEDADSEAERETRRVTAVAVMAAESGHMDMGTEALPSPDEAAAAAAAFAEVTTVTVANVGSSADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKSRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEAGVQPPCRVGHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPSTPTKAVSPTVVSGLEMSEHRSWLYLEEMVNSLLNTAQQLKTLFEQAKQASSCREAAVTQARMQVDTERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHVCGQSASVTVQADDVHVEESVIEKVAV 6CC4B10E914EFD83 deaf1_human Deformed epidermal autoregulatory factor 1 homolog Nuclear DEAF-1-related transcriptional regulator Suppressin Zinc finger MYND domain-containing protein 5 DEAF1 SPN ZMYND5 protein protein human Homo sapiens Human MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKKDSPKNITLLPATAATTFTVTPSGQITTSGALTFDRASTVEATAVISESPAQGDVFAGATVQEASVQPPCRASHPEPHYPGYQDSCQIAPFPEAALPTSHPKIVLTSLPALAVPPPTPTKAAPPALVNGLELSEPRSWLYLEEMVNSLLNTAQQLKTLFEQAKHASTYREAATNQAKIHADAERKEQSCVNCGREAMSECTGCHKVNYCSTFCQRKDWKDHQHICGQSAAVTVQADEVHVAESVMEKVTV 3BDFEDBF6AD4BDDE mecp2_mouse Methyl-CpG-binding protein 2 Mecp2 protein protein mouse Mus musculus Mouse MVAGMLGLREEKSEDQDLQGLRDKPLKFKKAKKDKKEDKEGKHEPLQPSAHHSAEPAEAGKAETSESSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTGRPKAAASEGVQVKRVLEKSPGKLVVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVHETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESTKAPMPLLPSPPPPEPESSEDPISPPEPQDLSSSICKEEKMPRGGSLESDGCPKEPAKTQPMVATTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS DIP protein Q9Z2D6 original sequence version: 154 62FD228F0118A49F deaf1-mecp2-1 4 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig. 1b mecp2-deaf1-1 3 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig. 1b 1-183 5 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association suppl. fig 1 To confirm the Deaf1-MeCP2 interaction, IgG, Deaf1, or MeCP2 antibodies were used to immunoprecipitate endogenous proteins from extracts of SKN-SH cells, hippocampal tissue or MEFs from Deaf1 knockout or wild-type mice. MeCP2 was detected in anti-MeCP2 precipitates in all samples, and was enriched when pulling down for Deaf1 in SKN-SH, Deaf1 +/+ MEF and WT hippocampal extracts, but not in Deaf1 −/− MEFs and Deaf1 KO hippocampal tissue (Supplementary Fig. S1). It was not possible to detect Deaf1 due to cross-reactivity with IgG heavy chain, and the lack of a suitable alternate Deaf1 antibody. Taken together, these results indicate that MeCP2 and Deaf1 proteins interact. deaf1-mecp2-2 2 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association suppl fig 1 To confirm the Deaf1-MeCP2 interaction, IgG, Deaf1, or MeCP2 antibodies were used to immunoprecipitate endogenous proteins from extracts of SKN-SH cells, hippocampal tissue or MEFs from Deaf1 knockout or wild-type mice. MeCP2 was detected in anti-MeCP2 precipitates in all samples, and was enriched when pulling down for Deaf1 in SKN-SH, Deaf1 +/+ MEF and WT hippocampal extracts, but not in Deaf1 −/− MEFs and Deaf1 KO hippocampal tissue (Supplementary Fig. S1). It was not possible to detect Deaf1 due to cross-reactivity with IgG heavy chain, and the lack of a suitable alternate Deaf1 antibody. Taken together, these results indicate that MeCP2 and Deaf1 proteins interact. deaf1-mecp2-3 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association suppl fig 1